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PENGARUH DURASI RADIASI UVC TERHADAP NILAI TOTAL PLATE COUNT (TPC) CILOK (EFFECT OF UVC RADIATION DURATION ON THE TOTAL PLATE COUNT (TPC) OF CILOK) Safari, Wulan Fitriani; Fariska, Dania Febriani; Syafaat, Mohamad
Journal of Medical Laboratory Research Vol. 4 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Cilok is a popular street food enjoyed by various groups, but it carries a high risk of microbial contamination. One of the parameters used to assess food safety is the Total Plate Count (TPC), with a maximum limit of 1 × 10⁵ CFU/g according to BPOM standards. UVC irradiation can be applied to reduce microbial contamination in cilok. The effectiveness of UVC radiation in inactivating bacteria is influenced by several factors, such as wavelength, distance from the light source, and exposure duration. This study aims to determine whether the duration of UVC radiation affects the TPC value of cilok. Cilok samples were collected from street vendors around Binawan University and exposed to UVC radiation for 0 minutes (control), 30 minutes, and 60 minutes. TPC testing was conducted using the Pour Plate method. The results showed that UVC radiation treatment reduced the TPC value of cilok. The highest TPC value was found in the untreated sample (1.8 × 10⁶ CFU/g), followed by samples exposed for 30 minutes (4.5 × 10⁵ CFU/g) and 60 minutes (2.1 × 10⁴ CFU/g). One-way ANOVA analysis indicated a significant difference among treatment groups (p < 0.05), suggesting that UVC radiation duration has a significant effect in reducing microbial counts in cilok.
Optimasi Suhu dan Waktu SSF untuk Produksi Bioetanol dari Sampah Daun Menggunakan Trichoderma Viride dan Zymomonas Mobilis Wulan Fitriani Safari
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

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Abstract

Foliage from gardens or parks is biomass whose organic fraction can be decomposed. Foliage can be utilized by converting it into bioethanol. Various studies have shown that Trichoderma viride and Zymomonas mobilis play a role in the production of bioethanol from biomass, but until now there has been no report on the production of bioethanol from foliage using a mixture of  T. viride and Z. mobilis. The purpose of this study was to determine the optimal SSF temperature and time for bioethanol production from foliage using T. viride and Z. mobilis. The fermentation process used the Simultaneous Saccharification and Fermentation (SSF) method with a mixture of T. viride and Z. mobilis with a composition of 5% : 5% (w/v). SSF was carried out at several variations of time and temperature, 60 hours, 72 hours, 80 hours and 96 hours for times and temperatures used are 32oC, 35oC and 38oC. The fermented bioethanol was purified by distillation and finally, the ethanol content was tested with GC. Bioethanol was successfully produced from foliage using T. viride and Z. mobilis with the highest ethanol content obtained in SSF with a temperature of 35oC and a time of 72 hours, which is 0.2151%.