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All Journal JETT (Jurnal Elektro dan Telekomunikasi Terapan) THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST BIOTROPIC The Journal of Tropical Biology Jurnal Elementer (Elektro dan Mesin Terapan) Jurnal Teknologi Biota JURNAL TEKNIK Medika Kartika : Jurnal Kedokteran dan Kesehatan Jaringan Laboratorium Medis Jurnal Biosense BIOLOVA Binawan Student Journal Biofaal Journal Public Health and Safety International Journal (PHASIJ) Journal of Medical Laboratory Research Jurnal Ecotipe (Electronic, Control, Telecommunication, Information, and Power Engineering) Prosiding Seminar Nasional Pemberdayaan Masyarakat (SENDAMAS) Jurnal Analis Kesehatan Kendari
Wulan Fitriani Safari
Universitas Binawan
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Kelimpahan Mikroba Tanah Asal Rizosfer Tanaman Sukulen Zulaika, Aidha; Wijayanti, Dian Rachma; Safari, Wulan Fitriani
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.340

Abstract

Plant host symbiosis is close related to soil microbial abundance. Soil microbial abundance will affect soil quality and fertility, thus will improve plant growth. Studies on soil microbial abundance in succulent plant rhizosphere, especially in Indonesia, are scarce. This study aims to observe soil microorganisms' existence and their abundance on succulent plant rhizosphere. This research used two primary methods to isolate Arbuscule Mycorrhizal Fungi (AMF) and actinomycetes. The spore extraction (soil separating) method was used to obtain AMF spores, followed by the root staining method to observe AMF infection on the plant roots. Serial dilution and pour plate method were used for isolation of Actinomycetes. The isolation results showed a high number of Actinomycetes distribution up to 3.3 x 106 Actinomycetes CFU/g from the plant Echinocactus grusonii, while AMF spores displayed the most elevated number up to 47 spore/25g from the plant Deuterocohnia sp. The percentage of AMF root infection is covered by 27,9% median. AMF identification was based on spore. The result showed an abundance presentation of Actinomycetes in each soil sample. AMF and Actinomycetes existence displayed symbiotic interaction between succulent plants and soil microorganisms. AMF and actinomycetes play the role of endophytes that help the growth of cacti, generally have a dry growing environment, and limited nutrition by this symbiotic interaction. Actinomycetes distribution in rhizospheric soil will increase plant growth-promoting factors activity. Eventually, the implications of research results are to explore the abundance and biodiversity of soil microorganisms from succulent plant rhizosphere that lacked exploration.
Identifikasi Bakteri Dari Sampel Darah Pasien COVID-19 Di RSUD Ciawi Kabupaten Bogor Safari, Wulan Fitriani; Wardhiati, Sri; Dewi, Almatin Puspa
Journal of Medical Laboratory Research Vol. 2 No. 2 (2024): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36743/jomlr.v2i2.712

Abstract

Coronavirus Disease 2019 (COVID-19) is a disease caused by the Severe Acute Respiratory Syndrome Corona Virus 2 virus called SARSCov-2, this virus attacks the respiratory tract and creates a risk of infection in COVID-19 patients. Bacterial infections are known to complicate viral infections of the respiratory tract and are associated with worsening conditions in COVID-19 patients. The aim of this research is to identify bacteria from blood samples of COVID-19 patients at Ciawi Hospital, Bogor Regency. This type of research is quantitative descriptive which describes the types of bacteria from blood samples from COVID-19 patients. The samples in this study were patients who were confirmed (PCR) positive for COVID-19 who underwent blood culture microbiology examinations at Ciawi Regional Hospital. Data collection was carried out using the documentation study method. The research results showed that of the 243 COVID-19 patients who underwent blood culture examination, 63 samples showed positive results. In blood culture from 63 positive samples, 51 samples (80.9%) found Gram positive bacteria consisting of: S. hominis, S. haemolyticus, S. epidermidis, S. cohnii ureal, S. aureus, S. warneri and E. faecalis. There were 12 Gram Negative bacteria in blood culture (19.1%) consisting of A. baumannii, E. coli, Pseudomonas sp, K. pneumoniae, S. paucimobilis and E. aerogenes.
NILAI TOTAL PLATE COUNT (TPC) BUBUR BAYI HOME INDUSTRY Sumantri, Janan Salma Nabilah; Safari, Wulan Fitriani; ., Septiani
Biofaal Journal Vol 5 No 2 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i2pp123-129

Abstract

Complementary foods can be divided into two groups based on how they are made, namely instant and processed complementary foods. Instant complementary foods are products that are mass-produced and packaged by factories, such as instant baby porridge, and processed complementary foods are types of complementary foods that are prepared by mothers themselves at home with various creations and variations. Total plate count (TPC) is one of the parameters of microbial contamination in food. The aim of the study was to determine the total plate count value of home industry baby porridge samples (processed complementary food) in order to ensure the safety of consumption. This study was an analytical observational study. The TPC value was determined by counting the number of bacterial colonies that grew on PCA media with dilutions of 10-1 to 10-5. The collected data were tabulated and compared with the SNI 01-7111.4-2005 standard. The results of the study obtained the average TPC values as follows: sample A (1.15 × 104 colonies/gram); sample B (1.25 × 104 colonies/gram); sample C (2.32 × 104 colonies/gram); sample D (1.84 × 104 colonies/gram) and sample E (7.95 × 103 colonies/gram). It was concluded that all baby porridge samples tested had TPC values that exceeded the threshold set by SNI 01-7111.4-2005, which is 1×102 colonies/gram.
PERBANDINGAN SUHU PENYIMPANAN SUSU SAPI SEGAR TERHADAP TOTAL PLATE COUNT (TPC) Ajeng, Retno Ajeng Sugiyani; Wulan Fitriani Safari
JURNAL BIOSENSE Vol 7 No 02 (2024): Edisi Desember 2024
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v7i02.4439

Abstract

Abstrak Salah satu parameter pemeriksaan untuk menentukan cemaran bakteri pada susu adalah Total Plate Count (TPC). Banyaknya jumlah sampel yang harus diperiksa, kedatangan sampel yang terlambat, kerusakan pada alat dan keterbatasan jumlah tenaga laboratorium dapat menyebabkan tejadinya penundaan pemeriksaan TPC. Penundaan pemeriksaan dapat mempengaruhi kualitas susu sapi segar, terutama jika suhu penyimpanan tidak terjaga. Suhu yang terlalu tinggi mempercepat pertumbuhan bakteri sedangkan suhu terlalu rendah dapat menyebabkan pembekuan, memengaruhi hasil. Penting menjaga suhu optimal untuk meminimalkan pertumbuhan mikroorganisme hingga pemeriksaan dilakukan. Tujuan penelitian ini adalah untuk mengetahui perbandingan suhu penyimpanan susu sapi segar terhadap TPC pada pemeriksaan langsung, disimpan selama 24 jam pada suhu -11ºC, dan disimpan selama 24 jam pada suhu 5ºC. Penelitian ini merupakan penelitian eksperimental dengan pendekatan cross sectional. Penelitian dilakukan di Laboratorium Mikrobiologi Universitas Binawan menggunakan 9 sampel susu sapi segar. Hasil penelitian menunjukan hasil pemeriksaan TPC disimpan selama 24 jam pada 5ºC lebih tinggi dibandingkan pemeriksaan langsung dan disimpan selama 24 jam. Nilai TPC susu sapi segar pada pemeriksaan langsung sebesar 5,63×106 CFU/mL, disimpan selama 24 jam pada suhu -11ºC sebesar 5,56×106 CFU/mL, dan disimpan selama 24 jam pada 5ºC sebesar 8,33×106 CFU/mL. Uji statistik Paired T Test diperoleh nilai signifikansi yaitu 0,970; 0,471; dan 0,218 yang berarti sig > 0,05 yang berarti tidak terdapat perbedaan nilai TPC susu sapi segar pemeriksaan langsung, disimpan selama 24 jam pada suhu -11ºC, dan disimpan selama 24 jam pada suhu 5ºC. kata kunci: susu sapi segar; suhu penyimpanan; total plate count; dan waktu penundaan. ABSTRAK Banyaknya sampel yang harus diuji, keterlambatan kedatangan sampel, malfungsi peralatan, dan keterbatasan tenaga laboratorium dapat menyebabkan keterlambatan pengujian TPC. Keterlambatan tersebut dapat mempengaruhi kualitas susu sapi segar, terutama jika suhu penyimpanan tidak dijaga dengan baik. Suhu yang tinggi mempercepat pertumbuhan bakteri sedangkan suhu yang rendah dapat menyebabkan pembekuan sehingga mempengaruhi hasil. Sangat penting untuk menjaga suhu yang optimal untuk meminimalkan pertumbuhan mikroba sampai pengujian selesai. Tujuan dari penelitian ini adalah untuk membandingkan suhu penyimpanan susu sapi segar terhadap TPC saat diuji secara langsung, disimpan selama 24 jam pada suhu -11ºC, dan disimpan selama 24 jam pada suhu 5ºC. Penelitian ini merupakan penelitian eksperimental dengan pendekatan cross sectional, yang dilakukan di Laboratorium Mikrobiologi Universitas Binawan dengan menggunakan 9 sampel susu sapi segar. Hasil penelitian menunjukkan bahwa hasil pemeriksaan TPC yang disimpan selama 24 jam pada suhu 5ºC lebih tinggi dibandingkan pemeriksaan secara langsung dan disimpan selama 24 jam. Nilai TPC susu sapi segar pada pemeriksaan langsung adalah 5,63×106 CFU/mL, setelah 24 jam penyimpanan pada suhu -11ºC adalah 5,56×106 CFU/mL, dan setelah 24 jam penyimpanan pada suhu 5ºC adalah 8,33×106 CFU/mL. Analisis statistik Uji T Berpasangan menghasilkan nilai signifikansi 0,970; 0,471; dan 0,218 yang menunjukkan bahwa sig > 0,05, artinya tidak ada perbedaan signifikan nilai TPC antara pemeriksaan langsung, penyimpanan selama 24 jam pada suhu -11ºC, dan penyimpanan selama 24 jam pada suhu 5ºC. Kata kunci : susu sapi segar; suhu penyimpanan; Total Plate Count; dan waktu tunda.
Hubungan Antara Kadar HbA1c dengan Kadar Trigliserida terhadap Pasien Prolanis Diabetes Melitus Tipe 2 di Laboratorium Klinik Kimia Farma Depok Putri, Riantika Lutfiana; Aryani, Desi; Safari, Wulan Fitriani
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.12189

Abstract

Diabetes Mellitus (DM) is a group of metabolic disorders characterized by elevated blood glucose levels resulting from abnormalities in insulin secretion, insulin action, or both. Patients with Type 2 DM are at a higher risk of developing cardiovascular diseases, often indicated by dyslipidemia, which is characterized by elevated triglyceride levels, increased Low-Density Lipoprotein (LDL) levels, and decreased High-Density Lipoprotein (HDL) levels. This study employs an analytical observational design. The data obtained shows that controlled and uncontrolled HbA1c levels with normal triglyceride levels were found in 24.7% of the patients, while 75.3% had high triglyceride levels. The data were further analyzed using the Statistical Package for the Social Sciences 25 (SPSS 25) with a non-parametric test, specifically the Chi-Square continuity correction test, with a significance level of p 0.05. The Chi-Square test results indicated that H0 was rejected and Ha was accepted, with a p-value of 0.036 for the relationship between HbA1c and triglycerides. These results suggest a correlation between HbA1c levels and triglyceride levels in Prolanis Type 2 DM patients at Kimia Farma Clinical Laboratory in Depok. These findings can serve as an evaluation material for Prolanis DM Type 2 patients to control their glycemic levels by routinely monitoring their HbA1c and triglyceride levels.
Candida spp. Profile in the Oral Cavity of Infants and Children at the Cawang Integrated Health Post Tofiq, Hamim; Safari, Wulan Fitriani; Ritonga, Ahmad Fitra
Journal of Medical Laboratory Research Vol. 3 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36743/jomlr.v3i1.848

Abstract

Kandidiasis oral adalah infeksi kandidiasis yang cukup sering terjadi pada bayi dan anak. Agen penyebab kandidiasis bagian rongga mulut di antaranya yaitu Candida albicans, Candida parapsilosis Candida tropicalis, Candida glabrata, Candida krusei. Beberapa faktor dilaporkan mempengaruhi terjadinya kandidiasis pada bayi dan anak. Tujuan penelitian ini yaitu untuk mengetahui profil Candida pada rongga mulut bayi dan anak. Penelitian ini merupakan penelitian deskriptif dengan mengamati hasil kultur swab rongga mulut bayi dan anak pada media Hicrome Candida Differential Agar. Responden pada penelitian ini yaitu bayi dan anak yang melakukan pelayanan kesehatan di Posyandu Cawang pada periode bulan Mei 2023 sebanyak 30 responden. Hasil kultur swab rongga mulut bayi terdapat 4 Spesies Candida yaitu C. albicans, C. krusei, C. glabrata, C. parapsilosis. Hasil kultur swab rongga mulut anak terdapat 5 spesies Candida yaitu C. albicans, C. krusei, C. glabrata, C. parapsilosis, C. tropicalis. Spesies Candida pada rongga mulut anak lebih banyak dibandingkan pada rongga mulut bayi.
Rancang Bangun Alat Deteksi Salmonella sp. pada Susu Berbasis Electronic Nose Safari, Wulan Fitriani
Jurnal ELEMENTER (Elektro dan Mesin Terapan) Vol 11 No 1 (2025): Jurnal Elektro dan Mesin Terapan (ELEMENTER)
Publisher : Politeknik Caltex Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35143/elementer.v11i1.6447

Abstract

Contamination of pathogenic bacteria in milk can cause milk spoilage and become a gateway for disease transmission to humans. Salmonella sp. is one of the pathogenic bacteria that contaminates milk and can be transmitted to humans who consume the milk. The safety of milk in relation to foodborne diseases is an important thing to consider. Therefore, it is necessary to examine the contamination of pathogenic bacteria in milk. The purpose of this study was to design a Salmonella sp. detection tool in milk based on Electronic Nose (E-nose). The research was carried out through several stages, namely making block diagrams, mechanical design, Arduino design and tool testing. The design of the tool made using the MQ-136 sensor which will detect Hydrogen Sulfide (H2S) gas. The results of the tool test showed the reading results of the MQ-136 sensor for milk without Salmonella sp. culture of 92-118 ppm while the reading of the MQ-136 sensor for milk given Salmonella sp. culture was 126-166 ppm. The design of the Salmonella sp. detection tool in milk based on the electronic nose was successfully made. The test results showed that there was a difference in the reading results of the MQ-136 sensor.
Perbedaan Kadar Glukosa Darah Puasa Antara Sampel Serum Dan Sampel Plasma Heparin: Differences In Fasting Blood Glucose Levels Between Heparin Plasma Samples And Serum Samples Putri, Dian Eka; Indrayani, Anisa; Safari, Wulan Fitriani; Wirahkusuma, Dhinasty Armenia
Binawan Student Journal Vol. 7 No. 1 (2025)
Publisher : Direktorat Penelitian, Pengabdian Masyarakat, Dan Kerjasama Universitas Binawan (DPPMK Universitas Binawan)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54771/sbhzh716

Abstract

Sampel serum paling umum digunakan di praktek klinis, dibandingkan sampel plasma heparin yang lebih direkomendasikan. Kadar glukosa turun selama waktu pemrosesan serum. Penelitian ini bertujuan mengetahui perbedaan antara kadar glukosa darah puasa (GDP) sampel serum dan plasma heparin. Penelitian analitik cross sectional, melibatkan  40 subjek dewasa non-diabetes di Laboratorium Sunter Sisma Medical pada Desember 2023- April 2024. Pengukuran kadar GDP menggunakan metode GOD-PAP. Uji beda rerata kadar glukosa serum dan plasma heparin menggunakan T-paired test. Nilai bias (d) antara sampel dihitung. Kadar GDP serum lebih rendah dibandingkan plasma (p=0,000). Nilai bias glukosa serum 5,2% terhadap sampel plasma heparin; melebihi batas variasi biologis glukosa yang diperbolehkan (<2,2%). Terdapat penurunan kadar GDP serum dibandingkan GDP plasma heparin bahkan tanpa penundaan pemrosesan. Penelitian ini membuktikan penggunaan sampel plasma heparin lebih unggul mencegah penurunan glukosa secara invitro. Sampel serum seharusnya tidak digunakan untuk menggantikan sampel plasma pada pemeriksaan GDP.
Nilai Total Plate Count (TPC) pada Jajanan Pasar di Pasar Pondok Gede Kota Bekasi Yoelianto, Yassmin Athaya; Wulan Fitriani Safari; Achamadi
Jurnal Analis Kesehatan Kendari Vol. 7 No. 2 (2025): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 7 (2) Juni 2025)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v7i2.346

Abstract

Market snacks are food processed by food processors at the point of sale, whether it is sold directly or served as ready-to-eat food to the public. Market snacks can usually be found in traditional markets such as Pondok Gede Market in Bekasi City. Some of the factors that contribute to food contamination include food sources and marketing processes. This contamination can cause foodborne disesases. One of the parameters for food contamination test is Total Plate Count (TPC). The purpose of this study was to determine the TPC value of risol, dadar gulung, and lemper market snacks sold at Pondok Gede Market. The method in this study was analytic observational. The TPC test was carried out following SNI 01-2897-1992 with a modification in the diluent solution to 0.9% NaCL. The test used the pour plate method on Plate Count Agar (PCA) media incubated for 24-48 hours. The TPC value was determined by counting the number of colonies that grew on the media with dilutions of 10-1 to 10-5. The TPC value of sample 1 was 1.6 x 104 colonies/g, sample 2 was 5.1 x 107 colonies/g, sample 3 was 6 x 105 colonies/g, sample 4 was 1.1 x 104 colonies/g, sample 5 was 4.4 x 107 colonies/g and sample 6 was 1 x 106 colonies/g. The results of the study can be concluded that of the 6 samples tested, 4 samples exceeded the threshold and the other 2 samples were below the threshold set by BPOM RI No. 13 of 2019, namely with a maximum limit of 1 x 105 colonies/g for lemper samples and 5 x 104 colonies/g for dadar gulung and risol samples.
Hubungan Antara Kadar HbA1c dengan Kadar Trigliserida terhadap Pasien Prolanis Diabetes Melitus Tipe 2 di Laboratorium Klinik Kimia Farma Depok Putri, Riantika Lutfiana; Aryani, Desi; Safari, Wulan Fitriani
Jaringan Laboratorium Medis Vol. 6 No. 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.12189

Abstract

Diabetes Mellitus (DM) is a group of metabolic disorders characterized by elevated blood glucose levels resulting from abnormalities in insulin secretion, insulin action, or both. Patients with Type 2 DM are at a higher risk of developing cardiovascular diseases, often indicated by dyslipidemia, which is characterized by elevated triglyceride levels, increased Low-Density Lipoprotein (LDL) levels, and decreased High-Density Lipoprotein (HDL) levels. This study employs an analytical observational design. The data obtained shows that controlled and uncontrolled HbA1c levels with normal triglyceride levels were found in 24.7% of the patients, while 75.3% had high triglyceride levels. The data were further analyzed using the Statistical Package for the Social Sciences 25 (SPSS 25) with a non-parametric test, specifically the Chi-Square continuity correction test, with a significance level of p < 0.05. The Chi-Square test results indicated that H0 was rejected and Ha was accepted, with a p-value of < 0.036 for the relationship between HbA1c and triglycerides. These results suggest a correlation between HbA1c levels and triglyceride levels in Prolanis Type 2 DM patients at Kimia Farma Clinical Laboratory in Depok. These findings can serve as an evaluation material for Prolanis DM Type 2 patients to control their glycemic levels by routinely monitoring their HbA1c and triglyceride levels.
Co-Authors ., Septiani Achamadi Achmadi Achmadi Achmadi Achmadi Aidha Zulaika Ajeng, Retno Ajeng Sugiyani Almatin Puspa Dewi Anisa Marselina desi aryani Dewi, Almatin Puspa Dian Eka Putri, Dian Eka Dian Rachma Wijayanti Indrayani, Anisa Intan Kurniawati Pramitaningrum Mohamad Syafaat Putri, Riantika Lutfiana Ritonga, Ahmad Fitra Septiani Septiani Sumantri, Janan Salma Nabilah Syafrima Wahyu Tia Humaira Zahrah Tofiq, Hamim Trianto Haryo Nugroho Wardhiati, Sri Wirahkusuma, Dhinasty Armenia Wisnu Murti Yoelianto, Yassmin Athaya