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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Zuriat Jurnal Agro Agrin : Jurnal Penelitian Pertanian Jurnal Agrotek Indonesia Jurnal Ilmiah Wahana Pendidikan
Untung Susanto
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QTL Mapping of Yield, Yield Components, and Morphological Traits in Rice (Oryza sativa L.) Using SSR Marker Untung Susanto; Hajrial Aswidinnoor; Jajah Koswara; Asep Setiawan; Vitaliano Lopena; Lina Torizo; Virk S. Parminder
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (406.523 KB) | DOI: 10.24831/jai.v36i3.1376

Abstract

The experiment was aimed at identifying QTL (quantitative trait loci) controlling ten traits of yield, yield component and plant morphology of rice based on BC1F1 of IR75862-206-2-8-3-B-B-B//IR64 mapping population consisted of 115 plants.  It was arranged in Completely Randomized Design with three replicates.  Ninety three SSR markers spread across the twelve rice chromosomes were used to map the QTL.  These markers were mostly segregated according to Mendel Law except for fourteen markers.  There were eleven QTL detected in eight traits, i.e., heading date, flag leaf length, plant height, panicle length, panicle weight, seed set, weight of 100 grains, and grain weight per plant, meaning that one or two QTL were detected in each trait.  These QTL were located at chromosome 2, 3, 4, 6, 11, and 12.  Some QTL were located at the same chromosome even at the same location indicating the close association of the traits.  It also indicated that there were common QTL which were found across genetic background and specific QTL which were found at specific genetic background.  Further study was prospective for the molecular marker application in rice improvement.   Key words: QTL, agronomic traits, SSR, rice
Kandungan Fe dan Zn pada Beras Pecah Kulit dan Beras Sosoh dari Galur-Galur Padi Toleran Wereng Batang Cokelat Wage Ratna Rohaeni; Edi Supriadi; Untung Susanto; Tina Dewi Rosahdi
Jurnal Ilmu Pertanian Indonesia Vol. 21 No. 3 (2016): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.859 KB) | DOI: 10.18343/jipi.21.3.172

Abstract

In addition to carbohydrates, rice contains other minerals that function to growth and development of vital human organs. On the other hand, the content is influenced by genetic and milling processes. Indonesian Centre of Rice Research (ICRR) has genetic collection resistant to strains of the brown plant hopper (BPH). Genotypes were studied to assemble the pest-resistant varieties with high iron (Fe) and zinc (Zn) content. This study aims to determine the BPH-resistant lines that had high and stable Fe and or  Zn content. The study was conducted in XRF Laboratory, ICRR Sukamandi, West Java in 2015. Fe and Zn were determined by using XRF machine with a system of performance-based X-ray. Total 20 rice lines that resistant to BPH was used Fe and Zn content on brown and polished rice were evaluated duplo. The results showed that milling generally decreased the content of Fe and Zn at rate 56.31% and 20.54%, respectively. The highest Fe content was found in BP19564b-WBC-1-1-4-3 (12.25 ppm), while the highest Zn content in BP19564b-WBC-1-9-8-3 (26.35 ppm). Genotype BP19564b-WBC-1-9-4-2 and BP19564b-WBC-1-9-8-3 exhibited the lowest in reduction Fe and Zn content, respective, from brown to polished rice.
PENGUJIAN TOLERANSI PLASMA NUTFAH PADI TERHADAP CEKAMAN SUHU RENDAH PADA AGROEKOSISTEM GOGO Rina Hapsari Wening; Untung Susanto
Zuriat Vol 23, No 2 (2012)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v23i2.6873

Abstract

Penelitian ini bertujuan mengevaluasi plasma nutfah koleksi BB Padi terhadap cekaman suhu rendah pada agroekosistem gogo untuk mendapatkan genotipe yang toleran. Percobaan dilaksanakan di Desa Sikumpul Kecamatan Kalibening Kabupaten Banjarnegara (1100 mdpl) pada MK 2010. Materi yang diuji sebanyak 86 aksesi plasma nutfah padi koleksi BB Padi dengan varietas pembanding yaitu Sarinah, Barito, Tejo, Ciherang dan IR 64. Percobaan ditata sesuai dengan rancangan augmented 4 blok dengan petak percobaan berukuran 1 x 2,5 m2.  Benih ditanam secara langsung (tabela) pada jarak tanam 20 x 20 cm dengan 1 benih per lubang pada agroekosistem gogo. Hasil penelitian menunjukkan bahwa aksesi Cere Beureum, RUTTST858B-5-2-2-2-0-J dan Padi Gunung/Huma diindikasikan toleran terhadap cekaman suhu rendah karena memiliki umur bunga yang nyata lebih genjah dan sama dengan Tejo yang diduga tidak escape dari cekaman suhu rendah. Aksesi Cere Manggu, Ringgit, dan Bereum Tomang memiliki sifat adaptif di dataran tinggi karena memiliki nilai yang nyata lebih baik dibanding cek terbaik Tejo pada karakter bobot gabah per rumpun, gabah isi per malai, fertilitas malai, skore fertilitas malai, dan eksersi malai. Aksesi Ase Andele, Kantong dan Randa Kaya juga dapat dikatakan adaptif di dataran tinggi karena memiliki beberapa sifat komponen hasil yang lebih baik dibanding cek terbaik Tejo. 
Variabilitas Genetik dan Daya Gabung Umum Galur-Galur Murni Jagung Melalui Analisis Topcross Untung Susanto; Achmad Baihaki; Ridwan Setiamihardja; Totok Agung Dwi Haryanto
Zuriat Vol 12, No 1 (2001)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v12i1.6679

Abstract

Merakit sebuah varietas jagung hibrida memerlukan jumlah galur murni yang sangat besar, padahal hanya sedikit sekali galur yang memiliki daya gabung yang tinggi, untuk dapat digunakan sebagai tetua hibrida superior. Topcross adalah metode seleksi daya gabung umum galur murni yang efisien. Tujuan penelitian ini adalah untuk mengevaluasi variabilitas genetik dan daya gabung umum masing-masing galur murni untuk menyeleksi galur-galur murni terbaik yang akan dilanjutkan dalam uji dialel. Bahan dalam percoabaan ini adalah galur-galur murni populasi 3 dan populasi 4 diambil dari koleksi galur murni laboratorium Pemuliaan Tanaman Fakultas Pertanian Universitas Padjadjaran. Penelitian ekperimental dilaksanakan dalam tiga musim tanam. Musim pertama dilaksanakan untuk melakukan selfing terhadap galur-galur murni, musim kedua untuk melakukan persilangan dengan tester dan yang ketiga untuk menganalisa hibrida hasil topcross. Sejumlah 142 hibrida dianalisa untuk mengevaluasi penampilan galurgalur murni. Hasil percobaan mengindikasikan bahwa hampir seluruh karakter yang diamati memiliki variabilitas genetic yang luas (bobot panen kering, bobot pipilan, jamlah baris biji per tongkol, bobot 100 biji, jumlah daun, panjang batang dan buku tongkol produktif). Sepuluh galur murni direkomendasikan untuk uji lebih lanjut dalam persilangan dialel. Kesepuluh galur murni tersebut adalah : nomor kode 296, 58, 118b, 53, 207, 82, 20, 108, 301, and 276.
Seleksi dan indeks sensitivitas cekaman kekeringan galur-galur padi sawah tadah hujan Wage Ratna Rohaeni; Untung Susanto
Jurnal Agro Vol 7, No 1 (2020)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/3654

Abstract

Cekaman kekeringan hampir terjadi setiap tahun pada sawah tadah hujan. Salah satu teknologi untuk mengatasinya adalah dengan penanaman padi toleran kekeringan. Perakitan varietas toleran kekeringan terus dilakukan dengan mengevaluasi galur-galur yang ditujukan untuk stress kekeringan. Penelitian ini bertujuan untuk menyeleksi, mengevaluasi penurunan hasil, dan mengetahui indeks sensitivitas cekaman kekeringan pada galur-galur tadah hujan hasil seleksi generasi lanjut. Percobaan seleksi cekaman kekeringan dilakukan pada Maret – November 2016 dengan materi genetik sebanyak 135 galur dan Cek Mekongga yang ditanam pada kondisi stress kekeringan (61-72 centibar/Kpa) dan evaluasi galur – galur terseleksi dilakukan pada Februari – Juli 2017 dengan menggunakan rancangan acak kelompok 3 ulangan. Hasil kegiatan seleksi memperoleh sebanyak 19 galur terbaik memiliki toleransi terhadap stres kekeringan untuk dilanjutkan pada kegiatan evaluasi lebih lanjut di kondisi sawah tadah hujan kondisi optimum. Hasil penelitian evaluasi 19 galur menunjukkan sebanyak 4 galur toleran yaitu BP17586-2-0-JK-3-IND-2-SKI-10-PWK-1-SKI-2 (ISK 0.49), BP18354-1-2-JK-3-IND-1-SKI-3-PWK-1-SKI-1 (ISK 0.49), BP18360-2-3-JK-1-IND-1-SKI-7-PWK-2SKI-1 (ISK 0.16), dan BP18406c-JK-1-IND-0-SKI-3-PWK-2-SKI-1 (ISK 0.1). Galur BP18354-1-2-JK-3-IND-1-SKI-3-PWK-1-SKI-1 (Y = 4,77 ton ha-1) adalah galur terbaik yang memiliki potensi hasil tinggi lebih baik dari cek Inpari 38 Tadah Hujan dan memiliki toleransi terhadap cekaman kekeringan. Galur ini potensial untuk diuji lebih lanjut dan dikembangkan di sawah tadah hujan.                                                     ABSTRACTDrought stress almost occurs every year in rainfed rice fields. One of the technologies to overcome is by planting drought tolerant varieties rice. Therefore, the assembly of drought tolerant varieties is carried out continuously by evaluating lines intended for drought stress. This study aimed to select, evaluate the decline in yield, and determine the drought tolerance index of rainfed lines resulting from advanced generation selection. Study-1 of drought stress selection experiment was carried out in WS 1 2016 (March – November) with 135 genetic lines and Mekongga as susceptible checks and Inpari 38 as resistant check planted in drought stress conditions (61-72 centibar / Kpa), and study-2 was an evaluation of selected strains carried out in WS 1 2017 (February – July) with 19 selected lines and 3 checks (Mekongga and Ciherang as susceptible check and Inpari 38 as resistant checks) by using a randomized block design with 3 replications. The results of the selection activities (Study-1) obtained as many as 19 of the best lines which had tolerance to drought stress and these lines were continued in further evaluation activities in rainfed lowland with optimum conditions (Study-2). The results showed that 4 of 19 lines were tolerant namely BP17586-2-0-JK-3-IND-2-SKI-10-PWK-1-SKI-2 (DSI - drought stress sensitivity index = 0.49), BP18354-1 -2-JK-3-IND-1-SKI-3-PWK-1-SKI-1 (DSI 0.49), BP18360-2-3-JK-1-IND-1-SKI-7-PWK-2SKI-1 (DSI 0.16), and BP18406c-JK-1-IND-0-SKI-3-PWK-2-SKI-1 (DSI 0.1). The BP18354-1-2-JK-3-IND-1-SKI-3-PWK-1-SKI-1 (Y = 4.77 tons ha-1) line was the best strain that had the potential for high yield better than the Inpari 38 Rainfed Check and had tolerance to stress. This strain has the potential to be further tested and developed in rain-fed rice fields.
VISUALISASI GEL AKRILAMIDA SIDIK JARI DNA 49 GENOTIPE PADI (Oryza sativa L) MENGGUNAKAN MARKA SSR (Simple Sequence Repeat) Carolin Sonia Saiallagan; Muhammad Syafi’i; Muhammad Yamin Samaullah; Untung Susanto; Estria Furry Pramudyawardani; Desy Prastika
Jurnal Ilmiah Wahana Pendidikan Vol 8 No 8 (2022): Jurnal Ilmiah Wahana Pendidikan
Publisher : Peneliti.net

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.219 KB) | DOI: 10.5281/zenodo.6605393

Abstract

Micronutrient deficiency remains a major concern in Indonesia, demonstrating how critical it is to meet micronutrient requirements, particularly for Zn micronutrients. Zn can boost a child’s IQ and lessen the risk of bleeding during childbirth (Kennedy et al, 2003). Inpara 1, Inpara 2, Inpara 3, Inpara 4, Inpara 5, Inpara 7, Inpara 9, Aek Sibundong, Bahbutong, Baroma, Batu tei, Ciasem, Cibodas, Cibogo, Cigeulis, Ciherang, Cialamaya Muncul, Cisokan, Conde, Fatmawati, IR 64, and Ketonggo) and 10 SSR markers with high Zn content were used as genetic material (RM475, RM3322, RM441, RM5607, RM335, RM300, RM162, RM3331, RM8007, and RM243). Contamination and the leftover solution used in the DNA extraction and isolation method can generate smears on some rice genotyping samples
Penampilan Karakter Agronomi Galur-Galur Padi (Oryza sativa L.) Kandungan Zn Tinggi di Dataran Medium Savira Mayani; Elia Azizah; Yamin Samaullah; Untung Susanto
Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech) Vol 7 No 1 (2022): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture University of Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33661/jai.v7i1.5995

Abstract

The formation of high-zinc rice varieties that have a high zinc is still done conventionally namely by crossing rice line that have a high zinc. The study aimed to determine the agronomic performance, growth and genotype yield of several rice lines with high Zn content. The research was conducted in October 2020 to March 2021 at IP2TP Kuningan. The research using Augmented Design with 188 lines and 6 comparison varieties, namely A (Inpari IR Nutri Zinc), B (Inpari 45 Dirgahayu), C (Inpari 32 HDB), D (Ciherang), E (Jeliteng), F (Inpago Fortiz), then if there is real influential treatment carried out advanced analysis using LSI 5%. The result of the experiment showed that there variation in agronomic characters and genotypes significantly affect the production of some rice plants. Line 143 (IR 105947-B-59-1-1-0-1) showed the highest plant height, line 26 (IR 105739-B-26-1-2-SKI-1-SG-20-SG-1) showed the highest number of productive tillers, line 14 (IR 105739-B-26-1-2-SKI-1-SG-14-SG-1) showed the fastest flowering and harvesting age, line 176 (IR 123818-B-235-2-1-B-3) showed the highest panicle length, line 70 (IR 105730-B-79-1-4-SKI-1-2-3-2) showed the highest percentage number of filled grains per panicle, line 176 (IR 123818-B-235-2-1-B-3) showed the highest percentage number of empty grains per panicle, line 184 (IR15M1349 -SKI-2) showed the highest weight of 1000 grains, line 42 ((BP29762d-PWK-3-SKI-2-MR-7-1-1) showed the highest grain weight. Milled dry grain of 8 (IR 105774-B-46-2-1-SKI-2-SG-15-SG-G) lines is 3.09 t/ha, higher than all the comparison varieties.
KERAGAAN AGRONOMIS GALUR-GALUR PADI SAWAH TADAH HUJAN GREEN SUPER RICE (GSR) DI INDONESIA Untung Susanto; Umi Barokah
Agrin Vol 20, No 1 (2016): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2016.20.1.314

Abstract

This research was aimed to initially test 40 rainfed lowland dedicated GSR lines along with 3 checks, i.e.PSBRC68, Situbagendit, and Silugonggo. The trial was conducted in ICRR experimental station in Sukamandiwith irrigation only until 2 weeks after transplanting and during flowering. The trial was conducted during DS2012 following Randomized Complete Block Design of three replication in 1 m x 1 m plot size and planting spaceof 20 cm x 20 cm. Transplanting was conducted to 21 days old seedings. The results showed that identified fiveline that have higher yields than the best check Silugonggo ( 4.22 t/ha ), which Luyin 46 ( 5.18 t/ha ), 926 ( 5.12t/ha ), SACG - 7 ( 4.46 t/ha ), LH1 ( 4.36 t/ha ) and Weed Tolerant Rice ( 4.30 t/ha ). A total of three lines , namelyZX788 ( 84 HSS ), 08FAN4 ( 89 HSS ) and D100 ( 91 HSS ) has a ripe age is significantly more early maturity ofthe check is very early maturing Silugonggo ( 95 HSS ). GSR lines tested had similar agronomic characters withexisting varieties, among others, from 46.67 to 100.2 cm plant height, number of productive tiller 6-10 fruit,flowering age 56-86 HSS, or physiological maturity round 84 -102 HSS, filled grain 47-185 grains per panicle,1000 grain weight 17.94 to 32.34 g, and the results ranged from 0.95 to 5.18 t/ha.Key words: GSR, rainfed lowland, agronomic performance, yield ABSTRAKPenelitian ini bertujuan untuk menguji awal daya adaptasi 40 galur GSR untuk padi sawah tadah hujan(GSR-Rainfed Lowland/GSR-RFLL) yang diintroduksi dari IRRI sebagai salah satu set pengujian dalam INGER(International Network for Rice Genetic Evaluation) beserta 3 varietas cek, yaitu PSBRC68, Situbagendit, danSilugonggo. Pengujian dilakukan pada kondisi sawah irigasi di Kebun Percobaan BB Padi di Sukamandi, namundengan perlakuan kering fase vegetatif, yaitu pengairan diberikan hingga dua minggu setelah tanam dan pada saattanaman berbunga, sebagai simulasi kondisi kering di lahan tadah hujan. Penelitian dilakukan pada MK 2012menggunakan rancangan acak kelompok tiga ulangan pada plot berukuran 1 m x 1 m dan jarak tanam 20 cm x 20cm. Tanam pindah dilaksanakan pada saat bibit berumur 21 HSS. Hasil pengujian mengidentifikasi lima galuryang memiliki daya hasil lebih tinggi daripada cek terbaik Silugonggo (4,22 t/ha), yaitu Luyin 46 (5,18 t/ha), 926(5,12 t/ha), SACG-7 (4,46 t/ha), LH1 (4,36 t/ha) dan Weed Tolerant Rice (4,30 t/ha). Sebanyak tiga galur, yaituZX788 (84 HSS), 08FAN4 (89 HSS) dan D100 (91 HSS) memiliki umur masak yang secara nyata lebih genjahdari cek sangat genjah Silugonggo (95 HSS). Galur-galur GSR yang diuji memiliki karakter agronomi setaradengan varietas unggul yang telah ada, antara lain tinggi tanaman 46,67-100,2 cm, jumlah anakan produktif 6-10buah, umur berbunga 56-86 HSS, atau masak fisiologis sekitar 84-102 HSS, gabah isi per malai 47-185 butir,bobot 1000 butir 17,94-32,34 g, dan hasil berkisar 0,95-5,18 t/ha.Kata kunci: GSR, sawah tadah hujan, keragaaan agronomis, hasil
sidik jari DNA 30 genotipe padi (oryza sativa L) Berdasarkan marka SSR yang terpaut dengan kandungan Zn Kinasih Sekar Pamungkas; Muharram Muharram; Rommy Andhika Laksono; Untung Susanto
Jurnal Ilmiah Wahana Pendidikan Vol 8 No 10 (2022): Jurnal Ilmiah Wahana Pendidikan
Publisher : Peneliti.net

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.275 KB) | DOI: 10.5281/zenodo.6791677

Abstract

This study to determine the PIC (polymorphic information content), gene diversity, and rice genotype groupings tested based on SSR markers reported to be associated with high Zn content traits in 30 genotypes. used the SSR Marking Application (Simple Sequence Repeats The research was carried out from May 2021 to September 2021 at the Center for Rice Research in Sukamandijaya Village, Ciasem District, Subang Regency, West Java Province. The study used descriptive analysis by observing the DNA banding pattern using 9 SSR primers. The results showed that 50 alleles were detected in 30 rice genotypes with an average number of marker alleles of 5.55 and a range of 3-10 alleles per locus, the average frequency of major alleles was 0.46 with the lowest value of 0.20 at the RM234 marker and the highest value of 0. 67 at the RM8226 mark. Heterozygosity values ranged from 0.0 at RM137 to 0.73 at the RM234 rice SSR marker with a mean of about 0.29. The value of diversity is between 0.50 (RM 8226) to 0.86 (RM 234, RM223) with an average of 0.67. The PIC (polymorphic information content I) value ranged from 0.44 (RM 152) to 0.85 (RM337) with an average of 0.62. Phylogenetic analysis showed that 3o rice genotypes were divided into 6 main groups with a coefficient of 0.45. The first cluster consists of 8 genotypes of rice, the second cluster has 1 genotype of rice, the third cluster has 13 genotypes of rice, the fourth cluster has 3 genotypes of rice, the fifth cluster has 1 genotype and the sixth cluster consists of 4 genotypes of rice.
Co-Authors Achmad Baihaki Carolin Sonia Saiallagan Desy Prastika Edi Supriadi Elia Azizah Estria Furry Pramudyawardani HAJRIAL ASWIDINNOOR JAJAH KOSWARA Kinasih Sekar Pamungkas Lina Torizo Muhammad Syafii Muhammad Yamin Samaullah Muharram Muharram Ridwan Setiamihardja Rina Hapsari Wening Rommy Andhika Laksono Savira Mayani Setiawan, Asep S Tina Dewi Rosahdi, Tina Dewi Totok Agung Dwi Haryanto Umi Barokah Virk S. Parminder Vitaliano Lopena Wage Ratna Rohaeni Wage Ratna Rohaeni Yamin Samaullah