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The Endophytic Actinobacterial Toxicity Test of Ginger (Zingiber officinale Roscoe) Used The BSLT (Brine Shrimp Lethality Test) Method Sri Rahayu; Lenni Fitri; Yulia Sari Ismail
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 1 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i1.8027

Abstract: Cancer is the biggest cause of death in Indonesia. Cancer treatment efforts have been made but it could harm cancer patients. It is necessary to find another alternative from nature as an anticancer. This study aims to see the ability of endophytic actinobacterial isolates from ginger (Zingiber officinale) as an anticancer tested using the BSLT method (Brine Shrimp Lethality Test) by calculating the values of LC50 and LT50. The best value isolates from the BSLT method, then tested using phytochemical ethanol extract. The highest toxicity value was found in AJ2 isolates. LC50 and LT50 values of AJ2 isolates were 309.358 µg/ml and 11.879 hours. Phytochemical results of ethanol extract of AJ2 isolates were detected containing steroids, terpenoids, phenols, tannins, flavonoids, alkaloids, and saponins. AJ2 isolates which derived from ginger endophytic actinobacteria have potential as an anticancer because they have high toxic values that rised rapidly.Abstrak: Kanker menjadi penyebab kematian terbesar di Indonesia. Upaya pengobatan kanker sudah dilakukan namun memberikan dampak negatif bagi penderita kanker. Perlu dilakukan penemuan alternatif lain berasal dari alam sebagai antikanker. Penelitian ini bertujuan untuk melihat kemampuan isolat aktinobakteri endofit asal jahe (Zingiber officinale) sebagai antikanker. 7 isolat aktinobakteri endofit jahe diuji menggunakan metode BSLT (Brine Shrimp Lethality Test) dengan menghitung nilai LC50 dan LT50. Isolat nilai terbaik dari metode BSLT, selanjutnya diuji menggunakan fitokimia ekstrak etanol. Nilai toksisitas tertinggi terdapat pada isolat AJ2. Nilai LC50 dan LT50 isolat AJ2 adalah 309,358 µg/ml dan 11,879 jam. Hasil fitokimia ekstrak etanol isolat AJ2 terdeteksi mengandung steroid, terpenoid, fenol, tanin, flavonoid, alkaloid dan saponin. Isolat AJ2 yang berasal dari actinobacteria endofit jahe berpotensi sebagai antikanker karena memiliki nilai toksik yang tinggi dalam waktu singkat.

Perubahan morfologi larva nyamuk akibat pemberian larvasida bakteri kitinolitik Yekki Yasmin; Lenni Fitri
Jurnal Entomologi Indonesia Vol 10 No 1 (2013): April
Publisher : Perhimpunan Entomologi Indonesia

The aim of this research is to evaluate the change of Aedes aegypti L. and Culex spp. larvae morphology effected by chitinolitic bacteria as larvacide. This research was conducted in Microbiology Laboratory, Faculty of Math and Natural Science, University of Syiah Kuala. The sample was taken from several areas in Banda Aceh and Aceh Besar. The isolation was accomplished by plating 1 ml of sample water on chitin agar medium and incubating for 48–72 hours in 30 °C temperature. In the experimental method, three concentrations of chitinolitic bacteria (0.5, 1.0, and 1.5 ml) in 7 days observation were used as a treatment. The research resulted in 6 isolates having the potential to be developed firther as chitinolitic bacteria. The result shows that chitinolitic bacteria degrades exosceleton of second instar larvae of Aedes aegypti and Culex spp. Degradation of exoskeleton started on the 4th days and occurred at the thorax region. All exoskeleton from both species are completely broken on day six.

Isolation and Characterization of Thermophilic Bacteria as Cellulolytic Enzyme Producer from the Hot Spring of Ie Seuum Aceh Besar, Indonesia RUHUL KHALILA; lenni fitri; SUHARTONO SUHARTONO
Microbiology Indonesia Vol. 14 No. 1 (2020): March 2020
Publisher : Indonesian Society for microbiology

Cellulase enzymes can be isolated from thermophile bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to recover and characterize the isolates morphologically and biochemically followed by determination of the thermophile bacterial isolates potential as cellulolytic enzyme producers,. The sampling method in this research was conducted by a purposive sampling at temperature of 70 oC, 60 oC and 50 oC. Isolation of thermophilic bacteria was carried out on nutrient agar (NA) media. There were four isolates of thermophilic bacteria isolated recovered at 70 oC, five isolates at 60 oC, and seven isolates at 50 oC. Of the 18 isolates obtained, 15 of them were able to produce cellulase enzymes. Cellulase enzyme production can be determined by the presence of clear zones around bacterial colonies on CMC media after addition of 1% congo red drops and wash with 1 M NaCl. The highest five Cellulolytic Index (CI) values â€‹â€‹were obtained from isolates ISB75; ISB64; ISB52; ISB54; ISB56 that were 1.23; 2.22; 1.39; 1.59; 1.10, respectively. Biochemical tests carried out on 5 isolates with the highest cellulolytic index values showed that the bacterial isolate were suspected to be from the genera of Bacillus sp.

Biofermentasi Urine Sapi Menjadi Pupuk Organik Cair di Kelompok Usaha Peternakan Sapi Masyarakat Desa Pasie Lamgarot Kecamatan Ingin Jaya Kabupaten Aceh Besar Firdus, Firdus; Fitri, Lenni; Salim, M. Nur; Fauziah, Fauziah
JURNAL PENGABDIAN PEMBANGUNAN PERTANIAN DAN LINGKUNGAN (JP3L) Vol 2 No 1 (2024): JURNAL PENGABDIAN PEMBANGUNAN PERTANIAN DAN LINGKUNGAN (JP3L): Volume 2 Nomor 1,
Publisher : LEMBAGA KAJIAN PEMBANGUNAN PERTANIAN DAN LINGKUNGAN (LKPPL)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62671/jp3l.v2i1.55

Indonesia merupakan negara yang tingkat kesuburan tanah memprihatinkan. Diperkiran Indonesia hanya memiliki tanah yang masih subur sekitar 30%, artinya sekitar 70% tanah di Indonesia sudah tidak subur, sehingga memperburuk usaha bercocok tanam para petani di Indonesia. Pemerintah terus memproduksi pupuk terutama pupuk anorganik, keadaan ini menambah permasalahan terhadap kesuburan tanah bila digunakan dalam waktu berkepanjangan, tanpa diimbangi pemberian pupuk organik. Karena itu diperlukan produksi pupuk organik, mengingat bahan baku sebagai sumber pupuk organik banyak tersedia di lingkungan, baik di lingkungan pertanian maupun lingkungan peternakan. Salah satu bahan baku pupuk organik dari kendang ternak adalah urine yang dapat diolah menjadi Pupuk Organik Cair (POC). Sehubungan dengan hal itu, telah dilakukan pelatihan kepada masyarakat peternak di Desa Pasie Lamgarot Kecamatan Ingin Jaya, Kabupaten Aceh Besar, Provinsi Aceh. Kegiatan tersebut berjalan dengan lancar, masyarakat dan perangkat desa menyambut baik terhadap kegiatan tersebut. Para peternak aktif dan serius mengikuti pelatihan mulai dari pembekalan materi sampai pelaksanaan pembuatan Pupuk Organik Cair dari urine sapi. Hasil analisis laboratorium pupuk organik cair urine sapi hasil pengabdian adalah Nitrogen (N) : 0,05 %, Fosfor (P2O5) : 0,0169 %, Kalium (K) : 0,0043 mg/L, pH : 4,29.

Production of Lipase Enzyme by Marine Actinobacteria With Various pH and Temperature Aulia, Muhammad Rizqi; Agustriana, Eva; Fitri, Lenni
Elkawnie: Journal of Islamic Science and Technology Vol 10, No 2 (2024)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v10i2.16724

Abstract: The demand for enzymes as biocatalysts in industry is very high. Research and development of different types of enzymes from different sources has started. One very important enzyme to study is the enzyme lipase. Lipase enzymes are enzymes of the hydrolase class that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Lipases are found in a variety of sources including animals, plants, and microorganisms. Marine microorganisms, including marine actinobacteria, cannot be separated from this enzyme source's research and development process. The purpose of this study was to obtain a test protocol and optimal pH and temperature conditions for the hydrolysis reaction by lipase enzymes from marine actinobacteria. Optimal pH and temperature conditions for hydrolysis reactions by lipase enzymes from marine actinobacteria using spectrophotometry at different pH values and different temperatures of 3, 4, 5, 6, 7, 8, 9, and 10. Temperatures of 30 °C, 40 °C, 50 °C, 60 °C, 70 °C, 80 °C and 90 °C are measured at a wavelength of 405 nm. The results showed that the activity of the lipase enzyme at pH 9 with Tris-HCl buffer was the optimum pH, and the temperature of 70°C was the optimum temperature for the lipase hydrolysis reaction of marine actinobacteria.Abstrak: Kebutuhan enzim sebagai biokatalisator dalam bidang industri sangat tinggi. Berbagai macam enzim dari beragam sumber sudah mulai diteliti dan dikembangkan. Salah satu enzim yang sangat penting untuk diteliti adalah enzim lipase. Enzim lipase merupakan enzim golongan hidrolase yang mengkatalis proses hidrolisis trigliserida menjadi gliserol dan asam lemak bebas. Lipase dapat ditemukan dalam berbagai sumber seperti pada hewan, tumbuhan, dan mikroorganisme. Mikroorganisme laut tidak terlepas dari proses penelitian dan pengembangan sumber enzim ini termasuk Actinobacteria laut. Tujuan penelitian ini adalah untuk memperoleh protokol uji dan kondisi pH dan temperatur optimum reaksi hidrolisis oleh enzim lipase dari Actinobacteria laut. Kondisi pH dan temperatur optimum reaksi hidrolisis oleh enzim lipase dari Actinobacteria laut dilakukan secara kuantitatif dengan metode spektrofotometri pada variasi pH 3, 4, 5, 6, 7, 8, 9, dan 10 dan variasi suhu 30°C, 40°C, 50°C, 60°C, 70°C, 80°C, dan 90°C diukur pada Panjang gelombang 405 nm. Hasil menunjukkan bahwa aktivitas enzim lipase pada pH 9 menggunakan buffer Tris HCl merupakan pH optimum dan temperatur 70°C merupakan temperature optimum reaksi hidrolisis enzim lipase dari Actinobacteria laut.

Utilization of fermented coffee husk feed using Aspergilus niger on histological features of tawes (Barbonymus gonionotus) Dhani, Desy Nurrahma; Fitri, Lenni; Firdus, Firdus
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 13, No 3 (2024): DECEMBER 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.3.40864

Coffee husk is one of the resources that has the potencial to be used as raw material for tawes (Barbonymus gonionotus) feed. The addition of Aspergillus niger in this study is known to increase the protein content of coffee husk from 15.99% to 21.75%. This study aims to determine the effectiveness of giving fermented coffee husk using Aspergillus niger on the histology of Tawes intestine. This study used a completely randomized design (CRD) with 5 treatments of coffee husk addition, namely 0%, 10%, 20%, 30%, and 40%, and 4 repetitions. The results showed that the addition of 30% fermented coffee husk into the feed produced the best intestinal histology picture with an average value of villi height of 243.21 40.02 m, basal width of 79.77 30.77 m and apical width of 69.09 14.61 m.Keywords:FermentationCoffee HuskBarbonymus gonionotusTawesAspergilus niger

Utilization of fermented coffee husk feed using Aspergilus niger on histological features of tawes (Barbonymus gonionotus) Dhani, Desy Nurrahma; Fitri, Lenni; Firdus, Firdus
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 13, No 3 (2024): DECEMBER 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.3.40864

Coffee husk is one of the resources that has the potencial to be used as raw material for tawes (Barbonymus gonionotus) feed. The addition of Aspergillus niger in this study is known to increase the protein content of coffee husk from 15.99% to 21.75%. This study aims to determine the effectiveness of giving fermented coffee husk using Aspergillus niger on the histology of Tawes intestine. This study used a completely randomized design (CRD) with 5 treatments of coffee husk addition, namely 0%, 10%, 20%, 30%, and 40%, and 4 repetitions. The results showed that the addition of 30% fermented coffee husk into the feed produced the best intestinal histology picture with an average value of villi height of 243.21 40.02 m, basal width of 79.77 30.77 m and apical width of 69.09 14.61 m.Keywords:FermentationCoffee HuskBarbonymus gonionotusTawesAspergilus niger

Endophytic Bacteria in Acalypha indica L. Leaves and Their Antimicrobial Activity Against Staphylococcus aureus and Candida albicans Syafitri, Aini; Fitri, Lenni; Suhartono, Suhartono
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.609

Background: The anting-anting plant (Acalypha indica L.) is used in herbal medicine in the treatment of various diseases. The leaf extract of this plant is known for its antimicrobial activity, but the antimicrobial properties of the endophytic bacteria within its leaves have never been reported. This research aims to determine the antimicrobial activity of endophytic bacteria from the leaves of the anting-anting plant. Materials and methods: The isolation of endophytic bacteria was performed using the spread plate method on nutrient agar (NA) media. Following isolation, the bacterial isolates were characterized through macroscopic and microscopic examination, as well as biochemical tests, which included indole production, hydrogen sulfide (H2S) production, motility, Simmons citrate utilization, methyl red-Voges-Proskauer (MR-VP) test, catalase test, and triple sugar iron agar (TSIA) test. Identification of the bacterial isolates was conducted according to Bergey's Manual of Systematic Bacteriology. Additionally, the antimicrobial activity of the isolates was assessed using the diffusion methodResults: Fourteen isolates of anting-anting leaf endophytic bacteria were obtained (coded as BEDA 1 to BEDA 14). The BEDA 5 isolate exhibited the largest inhibitory zone diameter against Staphylococcus aureus (31.48 mm), while BEDA 9 showed a significant inhibitory zone diameter against Candida albicans (17.84 mm). Conclusion: The two isolates (BEDA 5 and BEDA 9) exhibited significant antimicrobial activity, indicating their potential as promising candidates for alternative antimicrobial agents. These results suggest that endophytic bacteria from Acalypha indica may play an essential role in combating antibiotic resistance and in the development of new therapeutic strategies.Keywords: endophytic bacteria, characterization, antimicrobial activity, Acalypha indica

Evaluating the Economic Value of Above-Ground Biomass Carbon Stocks in the Urban Forests of Banda Aceh City Dharma, Wira; Yasmin, Yekki; Ariqah, Nada; Harnelly, Essy; Rizki, Alia; Fitri, Lenni; Amirunnas , Amirunnas; Rauzana, Anita; Zakaria, Rahmad
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.24368

Urban forests play a pivotal role in climate change mitigation by storing carbon in biomass. However, the economic valuation of this ecosystem service in Banda Aceh City remains underexplored. This study aims to quantify aboveground biomass carbon stocks and assess their economic value in six selected urban forests across the city. Non-destructive sampling was conducted using 20 × 20 m plots to measure tree diameter, estimate biomass using species-specific allometric equations, and calculate carbon content (C = 0.5 × B). A carbon price of US$50/ton was applied for economic valuation. The total carbon stock was estimated at 11,579 tons, with Putroe Phang Forest contributing the highest proportion (45%). The corresponding economic value was approximately IDR 3.42 billion. A strong positive correlation (R² = 0.9859) was observed between tree diameter and biomass. This study presents a novel economic perspective on urban forest carbon in Banda Aceh by integrating ecological data with financial valuation. The findings underscore the importance of incorporating carbon trading mechanisms into urban planning to strengthen ecosystem service assessments and climate policy implementation.

Toxicity And Antioxidant Activities of Endophytic Bacteria from Butterfly Pea (Clitoria ternatea Linn.) Zahrina, Yuni; Fitri, Lenni; Ismail, Yulia Sari; Dita, Sasmiati Farach
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.13360

Endophytic bacteria are bacteria that live in healthy plant tissues without causing damage. Several studies have reported that endophytic bacteria can produce active compounds similar to those secreted by their host and which potentially have medicinal value. Butterfly pea (Clitoria ternatea L.) was noted to be able to produce antioxidants and have toxicity potential from its compounds. Therefore, endophytic bacteria from butterfly pea have great potential to have antioxidant activity along with evaluating the toxicity level of the selected bacteria. This study aimed to determine the number of isolates, to characterize, and test the toxicity and antioxidant activities of endophytic bacteria from butterfly pea. Toxicity level was tested using the Brine Shrimp Lethality Test (BSLT) method while levels of antioxidants were tested using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. A total of fifteen endophytic bacteria were obtained and successfully purified. Based on the morphological observations, Gram staining, and biochemical test results, isolate EBT13 was determined to belong to the genus Bacillus. Isolate EBT13 was categorized as highly toxic, with the highest toxicity value with other bacterial isolates with an LC50 of 84 ppm and antioxidant activity with an IC50 value of 44.32 ppm. Based on the phylogenetic tree of 16S rRNA gene analysis, EBT13 belongs to the genus Bacillus, it forms a sister group with Bacillus pumilus with a bootstrap value of 100%. This study advances our knowledge of plant-microbe interactions by identifying a highly toxic, antioxidant-producing strain of bacteria of butterfly pea. The results have significance for the development of cytotoxic chemicals and natural antioxidants, which could advance biotechnological research and boost therapeutic purpose.

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