Setiaji, Asep
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Pendugaan Nilai Heritabilitas Bobot Badan pada Sapi Bali di BPTU-HPT Denpasar-Bali Andara, Dhita Indah; Setiaji, Asep; Edy Kurnianto
Jurnal Sain Peternakan Indonesia Vol 19 No 1 (2024)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.19.1.7-10

Abstract

Estimating heritability values can be used to implement selection programs and evaluate more targeted breeding programs in Bali cattle. This study aims to estimate the heritability value of birth weight, weaning weight and yearling weight of Bali cattle at Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak (BPTU-HPT) Denpasar Bali. The research material used is the recording data of 500 Bali cows originating from 24 males  (sire)with 500 Bali cows (dam) that produce offspring of as many as 500 calves. The data was secondary data, obtained by taking records of body weight data, including birth weight, weaning weight, and yearling weight, along with pedigree data of Bali cattle raised from 2017 to 2021 at BPTU-HPT Denpasar. Estimating the heritability value is done using a one-way unbalanced layout model. The results showed that the heritability values of birth, weaning, and yearling weights were 0.25±0.09, 0.23±0.08, and 0.16±0.07, respectively. Based on these results, it can be concluded that the heritability value of birth weight, weaning weight and yearling weight in BPTU-HPT Denpasar Bali is included in the moderate category so that it can be used as a selection guide to increase the productivity of Bali cattle as local Indonesian cattle.
Genetic Diversity of Simpo and Limpo Cattle Based on Five Blood-protein Locus Polymorphism Lestari, Dela Ayu; Sutopo, Sutopo; Setiaji, Asep; Kurnianto, Edy
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 3 (2022): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v9i3.25096

Abstract

Simpo and Limpo cattle is a crossbred cattle of Indonesian local cattle and exotic cattle that popular among traditional farmers in Indonesia. The objective of this study was to identify genetic diversity of Simpo and Limpo cattle based on blood-protein polymorphism. A total of 70 blood samples were collected from unrelated 35 heads of Simpo cattle and 35 heads of Limpo cattle from Grobogan dan Kendal population. The blood plasm was used to identify the blood proteins. Blood protein analysis was performed using Sodium Dodecyl Sulfate-Polyacrilamide Gel Electrophoresis (SDS-PAGE). Observed bands were used to perform allele interpretation of five locus, namely Pre-albumin (Pe-Alb), Albumin (Alb), Post-albumin (Po-Alb), Ceruloplasmin (Cp) and Amylase-1 (Am-1). Results showed there were polymorphism in all of the five blood protein locus in both cattle; 4 of 5 and 2 of 5 of the blood-protein locus studied showed deviation from Hardy-Weimberg Equilibrium (HWE) for Simpo and Limpo cattle, respectively and the average heterozygosity value (H) was 0.478 and 0.362 for Simpo and Limpo cattle, respectively. In conclusion, the study indicated that population of Simpo and Limpo cattle has high genetic diversity. Keywords: allele frequency; genotype frequency; heterozygosity
Identification of Gene Diversity of Melanocortin 4 Receptor for Commercial Rabbit Breeds Kamila, Firda Tasya; Kamalludin, Mamat Hamidi; Sutopo, Sutopo; Lestari, Dela Ayu; Setiatin, Enny Tantini; Agustine, Ananda Dwi; Latifa, Alfa Putri; Nabilah, Zata; Philco, Syaddad Verahry; Setiaji, Asep
Buletin Peternakan Vol 49, No 1 (2025): BULETIN PETERNAKAN VOL. 49 (1) FEBRUARY 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i1.99303

Abstract

Molecular genetic markers are regularly implemented in rabbit management for better breeding by selecting for specific genotypes. This study aimed to examine the diversity of the melanocortin-4 receptor gene in rabbits in Central Java, Indonesia. A total of 35 bucks were used in this study, comprising 10 New Zealand White, 11 Hyla and 14 Hycole. The DNA was extracted by Gene JET Whole Blood Genomic DNA extraction kit. Single Nucleotide Polymorphism (SNP) was detected by Polymerase Chain Reaction (PCR) and DNA sequencing. Hot carcass weight, cold carcass weight, reference weight and cut point 1-5 weight were among the parameters that were measured. This study showed that Melanocortin 4 Receptor (MC4R) genes were polymorphic, containing missense mutations and one SNP at 519G→A SNP 519GA had two alleles, A and G, with three genotypes (AA, GG, and AG). The rabbit population did not achieve HardyWeinberg Equilibrium (HWE) and showed a low level of genetic diversity as indicated by the heterozygosity results. The result of this study concluded that the frecuency AG as a genotype was higher than either GG or AA and A had a higher allele frequency than G. We recommended improving the validity of the data across various rabbit populations
Performa Pertumbuhan Tiga Varietas Ayam Kedu Umur 0–10 Minggu Akramullah, Muh; Kurnianto, Edy; Lestari, Dela Ayu; Setiatin, Enny Tantini; Setiaji, Asep
Jurnal Sain Peternakan Indonesia Vol 20 No 3 (2025)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.20.3.123-127

Abstract

This study aimed to evaluate the growth performance of Red-Combed Kedu (RCK), Black-Combed Kedu (BCK), and White Kedu (WK) chickens based on sex during the early growth phase (0 to 10 weeks of age). A total of 136 Kedu chickens were raised under uniform management conditions, consisting of 99 RCK (65 roosters and 34 hens), 29 BCK (5 roosters and 24 hens), and 8 WK (6 roosters and two hens). Body weight was recorded weekly and analysed using a two-way General Linear Model (GLM) in SAS to assess the effects of chicken variety, sex, and their interaction. The Shapiro–Wilk test confirmed that the data were normally distributed (p > 0.05). The results showed that sex had a significant effect on body weight from weeks 3 to 10 (p < 0.05), with roosters consistently exhibiting higher weights than hens. Significant differences among chicken varieties were observed only in the hen group at weeks 9 and 10, where BCK hens had higher body weights (p < 0.05) than WK hens, while RCK hens showed intermediate values. In contrast, no significant differences were found among rooster groups at any age. These findings suggest that genetic factors play an essential role in influencing growth performance in Kedu chickens, particularly in hens. This information provides a valuable foundation for breeding selection and the conservation of local chicken genetic resources.