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ISOLASI DAN KARAKTERISASI BAKTERI PENDEGRADASI PESTISIDA DICOFOL DARI TANAH SAWAH DI KABUPATEN KARAWANG Anindita Ayu Pratiwi; Agung Suprihadi; Budi Raharjo; Priyo Wahyudi; Sih Parmiyatmi
Jurnal Akademika Biologi Vol. 1 No. 1 Oktober 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Karawang was the center of rice production, but rice production in paddy fields in Karawawang often faces obstacle hopper pest that caused crop failure. These condition prompted the farmers to using dicofol pesticides excessively, thus caused pollution of pesticides. The purpose of this research were get the isolates bacteria that could degrade the pesticide dicofol in Karawang paddy fields and discovered characteristics of bacteria that have potential to degrade pesticides. The research was conducted with the isolation and characterisation of bacteria from Karawang paddy soil, the characterisation of isolates bacteria by observation morphological and phenotypic include staining Gram and biochemical tests, test isolates bacteria resistence of dicofol, measuring the growth curve and High Performance Liquid Chromatography (HPLC) for discovering of decreased dicofol concentration during 24, 48, and 72 hours of incubation time. The result is six isolates, that are AA1, AA2, AA3, AA4, AA5, and AA6. Isolates AA1 is suspected as genus Phenylobacterium, isolates AA3 and AA6 are suspected as genus Pseudomonas, AA4 isolates is suspected as genus Bacillus, isolates AA5 is suspected as genus Alcaligenes, but isolates AA2 not yet be identified. The results indicate that isolate AA6 has most resistent to dicofol and most number of cells bacteria, that is 7.9x108. Isolate AA6 is also the most effective in degrading dicofol as much as it can reduce the concentration in amount of 76.0 ppm (84.45%) in MSM medium + 90 ppm dicofol. Keywords : Isolation, Characterisation, Bacteria, Resistention, Degradation, Dicofol Pesticide

EKPLORASI BAKTERI SELULOLITIK DARI CAIRAN RUMEN SAPI PERANAKAN FRIES HOLLAND (PFH) DAN LIMOUSINE PERANAKAN ONGOLE (LIMPO) Sekar Ayu Yogyaswari; MG. Isworo Rukmi; Budi Raharjo
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Cellulose is the largest component of plant materials which is the most abundant organic compound in nature. The degradation of cellulose carried out by cellulase enzymes which is consisting of three components, i.e. , endoglucanase, exoglucanase, and β-glucosidase with glucose as the end product. Cellulase is very useful in industry and agriculture, such as paper, beer and brewing industries, for improving the quality of forages, organic material decomposer, and play an important role in the bioconversion of cellulose into various chemical commodities. The aims of this study was to get cellulolytic bacteria from cow’s rumen fluid which has high cellulolytic activity. Isolation of cellulolytic bacteria from cow’s rumen fluid were done by direct plating on CMC media. The cellulolytic activity values of the bacterial isolates were examined by determining cellulolytic index. The determination of cellulase activity were carried out by DNS method while the total protein contents by Lowry method. Identification of the bacteria isolates were done by observation of colony morphology, microscopic observation, and biochemical tests. Six potential cellulolytic bacterias were obtained in this study and all isolates identified as Bacillus. The highest cellulase specific activity shown by Fh-9 and Lo-8 isolates in 8 hours incubation.Keywords: cellulolytic bacteria, cellulose, cellulase.

PERTUMBUHAN ISOLAT RHIZOBAKTERI PELARUT FOSFAT DARI TANAMAN PADI DI MAYONG, JEPARA PADA MEDIA LIMBAH RUMAH PEMOTONGAN HEWAN DAN AIR KELAPA Rutty Wulandari; Agung Suprihadi; Budi Raharjo
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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The farmers are now turning towards the use of biofertilizer. The biofertilizer is living microbes applied to the soil in order to help facilitate or provide certain nutrients for plants. Previous study found that B4 isolate has proven capable of solubilizing phosphate so that it could be used as an agent that was inoculated in biofertilizer. The fertilizer can be formulated by modifying the alternative growth media in the liquid form that has potential, which is slaughterhouse waste and coconut water. Both media contain organic matters which support the growth of bacteria. This study aimed to test the potential of slaughterhouse waste and coconut water as a medium for the growth of B4 isolate. Methods of research conducted by Randomized Block Design, in which consists of five treatments namely P1 (100% coconut water), P2 (25% slaughterhouse waste & 75% coconut water), P3 (50% slaughterhouse waste & 50% coconut water), P4 (75% slaughterhouse waste & 25% coconut water), and P5 (100% slaughterhouse waste). The measured variable was the number of bacteria in the organic waste media during the 48 hours incubation period. Data on the number of bacteria was analysed by ANOVA test then continued with Duncan and LSD further tests. The results showed that P4 treatment significantly different from other treatments, with the highest number of bacteria (1,9 x 1011CFU/mL) at 18 hours incubation. The density of bacterial population in the five formula medias every 3 hours during the 48 hours incubation period showed significantly different, except at 0, 3, 12, and 45 hours incubation. Key words: biofertilizer, phosphate, slaughterhouse waste, coconut water

AKTIVITAS SPESIFIK SELULASE Serratia marcescens DENGAN VARIASI KONSENTRASI AMONIUM SULFAT ((NH4)2SO4) DAN pH Prawatya Cahyani; W Wijanarka; Budi Raharjo
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Cellulose is a component found in the cellular structure in almost all plant matter, its existence considered to be the most abundant on earth, and even excreted by some bacteria. Cellulose degradation is performed by cellulase enzymes consisting of three components, namely, endoglucanase, exoglucanase, and β-glucosidase enzyme with glucose as the final product. Cellulase utilization is often used in the textile, food and paper industries, whereas in the field of pharmaceuticals, cellulase enzymes are used to maintain optimal digestive health or produce substances that act as binding tablets such as methylcellulose, ethylcellulose, and hydroxypropylcellulose. The purpose of this study is to determine the concentration of ammonium sulfate and optimum pH for cellulose specific activity of Serratia marcescens. Determination of cellulase activity was done by DNS method, while determination of protein content was done by Lowry method. This research uses Randomized Block Design (RAK) factorial pattern with two factors. The first factor was variation of ammonium sulfate concentration which consisted of (0%, 0,75% and 1%). The second factor is the variation of pH consisting of 6, 7, and 8. Each factor is repeated 3 times. The data obtained were analyzed using Analysis Of Variance (ANOVA). The results showed that the combination of ammonium sulphate concentration variation with pH was not optimum to increase cellulose specific activity of S. marcescens. Keywords: Cellulase, Ammonium sulfate, pH, Serratia marcescens

Biodegradasi Senyawa Hidrokarbon Oleh Strain Bacillus cereus(VIC) Pada Kondisi Salinitas Yang Berbeda Reza Auliarahman Bhaktinagara; Agung Suprihadi; Budi Raharjo
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Bacillus cereus has been noted as hydrocarbonoclastic microbe that has ability to degrade hydrocarbons in non-saline conditions and some often to be found on high salinity environtment conditions. The purpose of this research is to determine the ability of strains Bacillus cereus (VIC) were isolated from non-saline environment to degrade hydrocarbons in crude oil on the different salinity condition. Bacillus cereus (VIC) was inoculated on the medium that has contaminated by crude oil with salinity level of 0,3 , 9,4 , dan 19,6 and incubated for 15 days. Determination of microbial growth is by using Total Plate Count (TPC) method along with determination of Total Petroleum Hydrocarbon (TPH) percentage using gravimetry method for every 5 day. The determination of microbial growth showed that Bacillus cereus (VIC) has the tolerancefor salinity level up to 19,6 because it is able to grow to a density of 6.9 x 106 CFU/ml on the 15th day. Bacillus cereus (VIC) is also able to degrades hydrocarbons on crude oil pollutants that indicated from degradation of TPH percentage in the medium up to 21% during 15 days incubation period on the medium with salinity level of 19,6 . Biodegradation using Bacillus cereus (VIC) can increase TPH degradation on the medium up to 19,8% than TPH degradation because of weathering. Keywords: Biodegradation, Bacillus cereus(VIC), Total Petroleum Hydrocarbon (TPH), Salinity.

EVALUASI KEPATUHAN DAN RESPON MUAL MUNTAH PENGGUNAAN ANTIEMETIK PADA PASIEN KANKER PAYUDARA YANG MENJALANI KEMOTERAPI DI RSUD PROF. DR. MARGONO SOEKARJO Wahyu Utaminingrum; Lukman Hakim; Budi Raharjo
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 10 No. 02 Desember 2013
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pji.v10i2.797

ABSTRAK Kanker payudara merupakan salah satu kanker dengan insidensi terbanyak, terutama pada wanita. Perkembangan terapi banyak dilakukan untuk meningkatkan survival dan prognosis pasien kanker payudara. Variasi pilihan terapi kanker payudara diberikan dengan mempertimbangkan banyak faktor, meliputi usia, status menopausal, komorbid, stadium kanker, faktor biologis dan riwayat kemoterapi. Optimasi kualitas hidup selama terapi merupakan hal yang sangat penting. Chemotherapy Induced Nausea Vomitting (CINV) merupakan efek samping yang sering terjadi dan dapat mempengaruhi kualitas hidup pasien kanker yang melakukan kemoterapi. Penelitian ini bertujuan untuk Mengetahui respon mual muntah, kepatuhan pasien terhadap obat anti mual muntah yang diberikan dan hubungan antara kepatuhan penggunaan anti mual muntah dan respon mual muntah pasien kanker payudara di RSUD Prof. Dr. Margono Soekarjo terhadap obat anti mual muntah. Penelitian ini merupakan penelitian observasional yang dilakukan dengan rancangan studi deskriptif dan analitik melalui penelusuran data secara prospektif terhadap pasien kanker payudara di RSUD Prof. Dr. Margono Soekardjo. Hasil penelitian yang diperoleh menunjukkan bahwa Respon mual muntah merupakan respon terbanyak yang ditimbulkan oleh pemberian agen kemoterapi baik pada fase acute emesis (80%) dan delayed emesis (90%). Sebanyak 79% pasien patuh terhadap regimen antiemetik yang diberikan. Tidak ada hubungan antara kepatuhan penggunaan antiemetik dan respon mual muntah pada pasien kanker payudara di RSUD Prof. Dr. Margono Soekarjo. Kata kunci: kanker payudara, CINV, respon mual muntah, kepatuhan. ABSTRACT Breast cancer is one of the cancers with the highest incidence, especially in women. The development of therapy has been implemented to improve the survival and prognosis of breast cancer patients. Breast cancer treatment is given by considering many factors, including age, menopausal status, comorbidities, stage of cancer, biological factors and history of chemotherapy. Optimization of quality of life during treatment is very important. Chemotherapy Induced Nausea vomitting (CINV) is a common side effect and can affect the quality of life of cancer patients. The aims of this study are to know response of nausea vomitting, patients adherence, relationship between adherence and response of nausea vomitting. This study is an observational study conducted by descriptive and analytic study design in Prof. Dr. Margono Soekarjo hospital. The results of this study are the nausea and vomitting response are the most in the acute emesis phase (80%) and delayed emesis (90%). A total of 79% were adherent to antiemetics therapy. There is no relationship between adherence and the response of nausea vomitting in Prof. Dr. Margono Soekarjo hospital. Key words: breast cancer, chemotherapy induced nausea and vomitting, antiemetics response, adherence.

Kombinasi Obat Antituberkulosis Pasien Anak Rawat Jalan Askes di RSUD Prof.Dr.Margono Soekarjo Moeslich Hasanmihardja; Iskandar Sudirman; Budi Raharjo; Riris Nurmila D.
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 05 No. 01 April 2007
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

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Telah dilakukan penelitian tentang kombinasi obat antituberkulosis yang paling banyak digunakan pada pasien anak rawat jalan Askes di RSUD Prof.Dr. Margono Soekarjo Purwoketo. Tujuan penelitian ini adalah untuk mengetahui apakah kombinasi obat yang paling banyak digunakan tersebut berinteraksi. Data kombinasi obat diteliti dengan mengamati rekan medik pasien anak rawat jalan Askes. Data yang diambil meliputi pengamatan resep, tabulasi data yang kemudian dianalisis secara deskripstif non analitik. Hasil analisis menunjukan bahwa kombinasi obat antituberkulosis yang paling banyak digunakan adalah isoniazida dan rifampisin. Pasien yang diteliti sebanyak 179 pasien. 87,71% pasien potensial terjadi interaksi obat yang signifikan secara klinis, 49,04% mengalami kenaikan SGPT dan atau perbandingan SGOT terhadap SGPT akibat kombinasi obat, 1,29% manifes dan 1,29% meninggal. Dari hasil tersebut ternyata isoniazida dan rifamfisin mempunyai efek samping kombinasi obat hepatotoksik.

PENGGUNAAN ASSOSIATION RULE MINING DALAM PENETAPAN HARGA PROMOSI, STOK, DAN PENATAAN PRODUK PADA ETALASE Budi Raharjo
E-Bisnis : Jurnal Ilmiah Ekonomi dan Bisnis Vol 13 No 1 (2020): Jurnal Ilmiah Ekonomi dan Bisnis
Publisher : STEKOM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51903/e-bisnis.v13i1.282

In the first quarter of 2017, retail in Indonesia recorded a growth of 2.5%, while in 2018 the growth was only in the range of 1% -1.5%. The cause of the slow growth is the change in the consumption pattern of the people and it will continue at the beginning of 2018. In addition, the decreasing productivity of the community at the lower middle level. As a retailer, Anterah store also faces the same thing, so anticipating a decline in sales requires an analysis of the best-selling products and how to find out the relationship between the products purchased by consumers. The association relationship between these products will be used as the basis for product arrangement, so that the frequency of products that consumers often buy can be arranged closely together so that consumers do not have to look for them longer. Market basket analysis to determine the relationship between products sold simultaneously is used to explore association rules (Association Rule Mining) which will produce products that are purchased simultaneously as a consideration for product arrangement in Anterah Retail storefront. Meanwhile, the best-selling products will be explored using the Frequent Pattern Growth method in order to obtain a ranking list of the most purchased products by consumers. This analysis is used as a basis for considering product promotion. The test results on the sales sample data obtained an average value of minimum support = 0.0025, minimum confidence = 0.610, LaPlace = 0.9985, Gain = -0.006, p-s = 0.003, Lift = 103.82, Convicting 2.5285 with a processing time of 41.456 seconds.

Prototype Aplikasi Berbasis Mobile Pada Sistem Administrasi Tata Tertib Peserta Didik Menggunakan SMS Gateway Sri Handoko; Budi Raharjo
Jurnal Publikasi Ilmu Komputer dan Multimedia Vol 2 No 3 (2023): September : Jurnal Publikasi Ilmu Komputer dan Multimedia
Publisher : Pusat Riset dan Inovasi Nasional

The system used for the administrative management of the code of conduct on SMA Swadaya Surabaya are still semi-manual record-keeping and reporting that is used is still manual and only as Ms. Office only. And the notification of student violations to parents only by post. This has resulted in frequent errors in recording violations of conduct learners that is unsynchronized between the daily record-keeping violations committed students and the monthly reports made by BK. In addition, the creation of reports requires a long time. And also the difficulty of communication between the school with parents about violations of students. Based on the description of the conditions, then the research aims to design the system of the administration of the code of conduct learners using the SMS Gateway. The system is designed with PHP as programming language, and MySQL Database Server. The method development of the system that is used for the design of the system i.e. SDLC (System Development Life Cycle) consisting of planning systems, analysis systems, design systems, and implementation. The results obtained from this research that is the system of administration code of conduct learners using the SMS Gateway with Mobile Web-based that can help the process of recording and be reporting quick and accurate. This system can also be used as an intermediary of communication between the school and parents regarding violations were done by learners in school.

PROTOTYPE APLIKASI BERBASIS MOBILE PADA SISTEM ADMINISTRASI TATA TERTIB PESERTA DIDIK MENGGUNAKAN SMS GATEWAY Sri Handoko; Budi Raharjo
Jurnal Teknik Mesin, Elektro dan Ilmu Komputer Vol. 1 No. 3 (2021): November : Jurnal Teknik Mesin, Elektro dan Ilmu Komputer (TEKNIK)
Publisher : Pusat Riset dan Inovasi Nasional

The system used for the administrative management of the code of conduct on SMA Swadaya Surabaya are still semi-manual record-keeping and reporting that is used is still manual and only as Ms. Office only. And the notification of student violations to parents only by post. This has resulted in frequent errors in recording violations of conduct learners that is unsynchronized between the daily record-keeping violations committed students and the monthly reports made by BK. In addition, the creation of reports requires a long time. And also the difficulty of communication between the school with parents about violations of students. Based on the description of the conditions, then the research aims to design the system of the administration of the code of conduct learners using the SMS Gateway. The system is designed with PHP as programming language, and MySQL Database Server. The method development of the system that is used for the design of the system i.e. SDLC (System Development Life Cycle) consisting of planning systems, analysis systems, design systems, and implementation. The results obtained from this research that is the system of administration code of conduct learners using the SMS Gateway with Mobile Web-based that can help the process of recording and be reporting quick and accurate. This system can also be used as an intermediary of communication between the school and parents regarding violations were done by learners in school. Keywords: code of conduct, SMS Gateway, Web-Based. ABSTRAK Sistem yang digunakan untuk administrasi pengelolaan tata tertib pada SMA Swadaya Surabaya masih bersifat semi-manual yaitu pencatatan dan pembuatan laporan yang digunakan masih manual dan hanya sebatas Ms. Office saja dan pemberitahuan pelanggaran siswa ke wali murid hanya sebatas via pos. Hal ini mengakibatkan sering terjadi kekeliruan dalam pencatatan pelanggaran tata tertib peserta didik yaitu ketidaksingkronan antara pencatatan harian pelanggaran yang dilakukan siswa dengan laporan yang dibuat oleh BK. Selain itu, pembuatan laporan per bulan memerlukan waktu yang lama. Dan juga sulitnya komunikasi antara pihak sekolah dengan wali murid mengenai pelanggaran siswa. Berdasarkan uraian kejadian tersebut, maka penelitian ini bertujuan untuk merancang sistem administrasi tata tertib peserta didik menggunakan SMS Gateway. Sistem ini dirancang dengan menggunakan PHP sebagai bahasa pemprograman, dan basis data MySQL Server . Tahapan pengembangan sistem yang digunakan untuk merancang sistem ini yaitu SDLC (System Development Lyfe Cycle) yang terdiri dari perencanaan sistem, analisis sistem, desain sistem, dan implementasi. Hasil yang diperoleh dari penelitian ini yaitu sistem administrasi tata tertib peserta didik menggunakan SMS Gateway berbasis Web Mobile yang dapat membantu proses pencatatan dan pembuatan laporan yang cepat dan akurat. Sistem ini juga dapat digunakan sebagai perantara komunikasi antara pihak sekolah dan wali murid mengenai pelanggaran yang dilakukan oleh peserta didik di sekolah. Kata Kunci: tata tertib, SMS Gateway, Web Mobile

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