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All Journal VALENSI JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia The Journal of Pure and Applied Chemistry Research Jurnal Kimia Riset ALCHEMY Jurnal Penelitian Kimia Molekul: Jurnal Ilmiah Kimia Dinamika Journal : Pengabdian Masyarakat Jurnal Serambi Abdimas Jurnal Natural Darma Sabha Cendekia Molekul: Jurnal Ilmiah Kimia
Zusfahair Zusfahair
Jurusan Kimia Fakultas MIPA Universitas Jenderal Soedirman Purwokerto
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Other

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Development of Urea Biosensor Based on Immobilized Urease in Chitosan Cryogel Zusfahair Zusfahair; Dian Riana Ningsih; Elok Dwi Putri Lestari; Amin Fatoni
Molekul Vol 14, No 1 (2019)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.474 KB) | DOI: 10.20884/1.jm.2019.14.1.523

Abstract

The development of biosensors using biological components has an important role in detecting the disease early because it has good selectivity and accuracy. In this study, a biosensor which made is a urea biosensor, based on immobilization urease in chitosan using adsorption techniques, to measure urea levels by colorimetric analysis with bromothymol blue (BTB) as an indicator. The purpose of this study was to find out how to measure urea levels using biosensors based on urease immobilization in chitosan and find out the biosensor performance including optimum enzymatic reaction time, linearity, the limit of detection, repetition, and determination of disrupting compounds. The study began with the making of an immobilization supporting matrix using chitosan which was made in the form of cryogel through an ionic gelation process which adsorbs the urease enzyme. Cryogel urease catalyzes the hydrolysis of urea into NH4+ and CO2-. The reaction product was added with the BTB indicator, and the color change formed was measured using a spectrophotometer. The results showed that the performance of urea biosensors was good enough for urea level detection systems by producing enzymatic reaction times at 15 minutes, linearity at 0.9951, detection limit at 0.018 mM, not affected by the addition of 0.05 mM ascorbic acid and 0.4 mM uric acid. This urea biosensor can be used up to 5 repetitions.
Immobilization and Characterization of Bacillus Thuringiensis HCB6 Amylase in Calcium Alginate Matrix Zusfahair Zusfahair; Dian Riana Ningsih; Dwi Kartika; Amin Fatoni; Indah Permatawati
Molekul Vol 12, No 1 (2017)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.968 KB) | DOI: 10.20884/1.jm.2017.12.1.249

Abstract

Free enzyme in solution react with substrates to result in products which cannot be recovered for reuse. These problems can be overcome to a certain extent by the use of enzyme immobilization method. Immobilized enzymes are more robust and more resistant to condition changes. More importantly, the heterogeneous immobilized enzyme systems allow an easy recovery of both enzymes and products, multiple re-uses of enzymes, and continuous operation of enzymatic processes. Entrapment of enzymes in Ca-alginate is one of the simplest methods of immobilization. The aim of this research was to obtain the optimum condition of the making of immobilized amylase beads using a Ca-alginate bead and to determine its characteristics. The optimization of immobilized amylase beads includes variation of sodium alginates and variations of enzyme contact time with CaCl2. The characterization of immobilized amylase includes determination of optimum substrate concentration, optimum pH, and optimum incubation time as well as amylase stability test. Amylase activity was determined by using dinitro salicylic (DNS) method. The results showed that the optimum immobilized amylase obtained at alginate concentrations of 5% (w/v), contact time of 60 minutes and immobilization efficiency of 67.5%. Furthermore, immobilized amylase showed optimum substrate concentration of 1.5-2.5% (w/v), optimum pH of 6, an optimum incubation time of 20 minutes with the activity of 179.8 U/mL. The KM value for free amylase and immobilized amylases were 0.3 mM and 0.12 mM respectively. Vmax value for free amylase and immobilized amylases were 105.3 U/mL and 10.1 U/mL respectively. Immobilized Amylase can be used up to six times with the residual activity of 52.7%.
AMOBILISASI PROTEASE DARI Bacillus sp. BT 1 MENGGUNAKAN POLIAKRILAMIDA PROTEASE IMMOBILIZATION FROM Bacillus sp. BT 1 USING POLYACRYLAMIDE Zusfahair Zusfahair; Amin Fatoni
Molekul Vol 6, No 2 (2011)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.917 KB) | DOI: 10.20884/1.jm.2011.6.2.96

Abstract

Penggunaan protease pada umumnya, dalam bentuk enzim bebas yang hanya sekali pakai, sehingga biaya produksi yang melibatkan enzim ini menjadi mahal. Amobilisasi enzim dapat mengatasi masalah ini, yang memungkinkan penggunaan enzim berulang kali. Dalam penelitian ini, protease dari Bacillus sp. BT 1, yang diperoleh dari sumber air panas, diamobilisasi dengan jebakan menggunakan poliakrilamida. Ekstrak kasar dalam bentuk enzim protease bebas dan enzim amobil dikarakterisasi termasuk suhu optimum, pH optimum, waktu inkubasi dan stabilitas enzim amobil pada penggunaan berulang. Aktivitas protease diukur dengan menggunakan metode Kunitz yang modifikasi. Hasil penelitian menunjukkan waktu produksi optimum protease adalah 36 jam yang berada pada akhir fase eksponensial pertumbuhan bakteri. Amobilisasi ekstrak kasar protease Bacillus sp BT 1 dapat menjebak 47,18% dari protease. Suhu optimum protease bebas 60 oC dan meningkat menjadi 70 oC pada penggunaan protease amobil. Protease bebas dan protease amobil memiliki pH optimum yang sama yaitu 11. Protease amobil tidak kehilangan aktivitas secara signifikan sampai empat kali penggunaan.
EKSTRAK DAUN MANGGA (Mangifera indica L.) SEBAGAI ANTIJAMUR TERHADAP JAMUR Candida albicans DAN IDENTIFIKASI GOLONGAN SENYAWANYA Dian Riana Ningsih; Zusfahair Zusfahair; Diyu Mantari
Jurnal Kimia Riset Vol. 2 No. 1 (2017): Juni
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (667.616 KB) | DOI: 10.20473/jkr.v2i1.3690

Abstract

AbstrakCandida albicans adalah salah satu jamur yang dapat menyebabkan infeksi candidiasis. Salah satu bahan obat alami dari ekstrak tanaman yang berpotensi sebagai antijamur adalah ekstrak daun mangga (Mangifera indica L.). Penelitian ini bertujuan untuk mengetahui aktivitas antijamur daun mangga terhadap C. albicans, penentuan konsentrasi hambat tumbuh minimum (KHTM) dan mengidentifikasi golongan senyawa kimia dari ekstrak tersebut yang berpotensi sebagai antijamur. Daun mangga diekstraksi secara maserasi menggunakan pelarut metanol. Ekstrak metanol daun mangga yang dihasilkan dilakukan uji aktivitas antijamur terhadap C. albicans dengan menggunakan metode difusi. Setelah diketahui aktivitasnya, ekstrak metanol daun mangga kemudian ditentukan konsentrasi hambat tumbuh minimum (KHTM) dan diuji kandungan metabolit sekundernya dengan uji fitokimia. Hasil ekstraksi daun mangga dengan pelarut metanol menghasilkan ekstrak metanol dengan rendemen 10,55% (b/b) dan menghasilkan aktivitas antijamur dengan zona hambat terbesar pada konsentrasi 1000 ppm dengan zona hambat 8,12 mm. KHTM ekstrak metanol daun mangga terhadap C. albicans yaitu pada konsentrasi 65 ppm dengan zona hambat sebesar 0,64 mm. Berdasarkan hasil uji fitokimia ekstrak metanol daun mangga menunjukkan adanya senyawa golongan alkaloid, flavonoid, stereoid, polifenol, tanin, dan saponin. Kata kunci : antijamur, Candida albicans, KHTM, Mangifera indica L. 
Immobilization of urease from Phaseolus vulgaris L. seeds using calcium alginate as a support matrix ZUSFAHAIR ZUSFAHAIR; DIAN RIANA NINGSIH; AMIN FATONI; ELY SETIAWAN
Jurnal Natural Volume 22 Number 3, October 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (858.124 KB) | DOI: 10.24815/jn.v22i3.26056

Abstract

Urease is an enzyme that functions as a catalyst in the hydrolysis of urea into ammonia and carbon dioxide. The industrial sector has made extensive use of urease. To date, enzymes are used in free form, deemed less effective. Therefore, enzymes are used in immobilized form because they can be utilized repeatedly. This research aimed to isolate urease from kidney bean (Phaseolus vulgaris L.) seed and immobilize it using a Ca-alginate support matrix and a trapping technique. Eight days were devoted to germinating kidney bean seeds to begin the investigation. Isolation of crude urease extract from kidney beans was carried out using phosphate buffer pH 7. It was then immobilized with Ca-alginate at different concentrations of Na-alginate and contact times The crude free and immobilized urease extract was further characterized including pH, temperature and stability of repeated use. The urease activity was determined using the Nessler method using a spectrophotometer. The results demonstrated that urease immobilization from kidney bean seeds with a Ca-alginate matrix was most effective at a concentration of 5% Na-alginate and a contact period of 60 minutes, yielding a value of 5.92 U/mL. The optimal pH of free and immobilized urease was 7 and 8, respectively, and temperatures of 35 and 40 °C, respectively. The immobilization of urease from kidney bean seeds using a Ca-alginate support matrix increased the stability of recurrent use by fivefold, while the relative urease activity remained at 52%.
Pengenalan Elektrokimia untuk Analisis Vitamin C bagi Siswa SMA Amin Fatoni; Zusfahair Zusfahair; Dadan Hermawan
Darma Sabha Cendekia Vol 2 No 2 (2020): Darma Sabha Cendekia - Desember 2020
Publisher : Pasca Sarjana | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.405 KB) | DOI: 10.20884/1.dsc.2020.2.2.3721

Abstract

Mata pelajaran kimia telah dikenalkan ke siswa Sekolah Menengah Atas (SMA) maupun Universitas, namun demikian, tidak semua teori kimia tersebut dipraktekkan di laboratorium karena berbagai keterbatasan. Metode analisis kimia merupakan salah satu topik dalam mata pelajaran kimia yang diaplikasikan secara luas pada bidang kesehatan. Salah satu metode kimia yang menarik dan banyak aplikasinya adalah metode elektrokimia dalam mendeteksi suatu senyawa. Metode ini jarang dilakukan praktek langsung karena keterbatasan instrumen yang mahal. Kegiatan pengabdian kepada masyarakat bertujuan untuk mengenalkan siswa SMA teori dan praktek metode analisis kimia menggunakan instrumen potentiostat untuk analisis senyawa elektroaktif, misalnya kadar vitamin C. Kegiatan ini dilaksanakan dalam suasana pandemi Covid-19, sehingga jumlah siswa yang terlibat dibatasi yaitu 15 siswa setiap hari, dan dilaksanakan selama 2 hari. Hasil pengabdian kepada masyarakat menunjukkan peningkatan pengetahuan dan keterampilan dari para siswa yang dilatih.
Pelatihan Uji Formalin Menggunakan Smartphone dan Scanner untuk Siswa SMA Amin Fatoni; Mekar Dwi Anggraeni; Anung Riapanitra; Zusfahair Zusfahair
Darma Sabha Cendekia Vol 3 No 3 (2021): Darma Sabha Cendekia - November 2021
Publisher : Pasca Sarjana | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (438.058 KB) | DOI: 10.20884/1.dsc.2021.3.3.5000

Abstract

Sekolah Menengah Atas (SMA) sudah mengajarkan pembelajaran kimia, namun demikian, belum semua melaksanakan praktikum untuk lebih memahami ilmu kimia, karena keterbatasan saran terutama alat dan petunjuk praktikumnya. Keterbatasan instrumentasi yang mahal memicu para peneliti untuk memanfaatkan alat-alat sederhana disekitar kita seperti kamera smartphone, kamera poket, kamera DSLR dan alat pemindai dokumen (scanner) sebagai alternatif instrumentasi kimia. Kegiatan Pengabdian masyarakat ini dikenalkan teori untuk meningkatkan pengetahuan siswa dan praktik untuk meningkatkan keterampilan siswa tentang penggunaan kamera smartphone dan scanner sebagai alat analisis kadar formalin. Hasil kegiatan pengabdian kepada masyarakat menunjukkan pemberian materi menunjukkan peningkatan pengetahuan dari skor rata-rata 68 menjadi 80, sedangkan praktikum dikelas memberikan peningkatan pengetahuan alat, bahan dan prosedur analisis kimia menggunakan smartphonedan scanner dari skor rata-rata 54,7 menjadi 86
Ointment Formulation of Arumanis Mango (Mangifera indica L.) Leaf Extract with Chitosan Tripoliphosphate Matrix as Antibacterial Dian Riana Ningsih; Anung Riapanitra; Zusfahair Zusfahair; Uyi Sulaeman; Istinganatun Khoeriyah
Molekul Vol 18 No 1 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.1.5725

Abstract

This report presented the synthesis of Arumanis mango (Mangifera indica L.) leaf extract with chitosan tripolyphosphate matrix and its antibacterial activity. This research aimed to obtain an ointment formulation from mango leaf extract with chitosan tripolyphosphate matrix, to figure out the characteristics, including the particle morphology, and to determine the optimum formulation and the characterization of the antibacterial ointment. The research showed that extract morphology with chitosan tripolyphosphate was uneven-edge aggregates. Antibacterial tests were conducted on P. acnes and E. coli bacteria. The formula giving the greatest antibacterial activity was further utilized for the ointment preparations and then was characterized for 16 days. Formula C (chitosan and NaTPP 1: 0.0992(%)) gave the most excellent inhibition zone for P. acnes and E. Coli bacteria, at 7.94 mm and 10.02 mm, respectively. The obtained ointment preparation was white color homogeneous semi-solid with protective properties. The spreading power of the ointment was 5.25 – 6.25 cm, with the adhesive power of 1 – 5 seconds and pH of 6.0 – 6.4. The ointment's antibacterial activity was tested against P. acnes and E. coli bacteria using the formation of inhibition zone method. The activity of ointment prepared on day one against P. acnes and E. coli was at 14.03 mm and 14.24 mm, respectively, while the activity on day 16 against P. acnes and E. coli was at 9.33 mm and 9.98 mm, respectively.
Pemurnian Parsial dan Karakterisasi Urease dari Biji Kacang Panjang (Vigna unguiculata subsp sesquipedalis L.) Zusfahair Zusfahair; Dian Riana Ningsih; Amin Fatoni; Darul Santri Pertiwi
ALCHEMY Jurnal Penelitian Kimia Vol 14, No 1 (2018): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.14.1.13000.72-83

Abstract

Urease merupakam enzim yang digunakan dalam hidrolisis urea menjadi amoniak dan asam bikarbonat dan telah banyak digunakan dalam proses industri. Tujuan penelitian adalah isolasi dan pemurnian urease dari kacang panjang serta karakterisasinya. Penelitian dimulai dengan melakukan perkecambahan biji kacang panjang selama 8 hari. Kecambah biji kacang panjang selanjutnya diekstraksi dengan menggunakan buffer fosfat pH 7 dan dipisahkan menggunakan sentrifugasi sehingga diperoleh ekstrak kasar urease. Ekstrak kasar urease selanjutnya difraksinasi menggunakan aseton pada tingkat konsentrasi 20, 40, 60 dan 80%. Fraksi yang mempunyai aktivitas spesifik paling tinggi selanjutnya dianalisis menggunakan metode SDS-PAGE untuk menentukan berat molekulnya dan dikarakterisasi lanjut meliputi: pengaruh suhu, pH, konsentrasi substrat dan penambahan ion logam terhadap aktivitas urease. Aktivitas urease ditentukan dengan metode Nessler. Hasil penelitian menunjukkan aktivitas spesifik urease dari kacang panjang paling tinggi ditemukan pada fraksi aseton (FA) 20. Hasil analisis berat molekul dengan metode SDS-PAGE diperoleh beberapa pita protein yang diduga berukuran sekitar 25 KDa dan 17 KDa. Kondisi optimum dari aktivitas urease diperoleh pada suhu 30 ºC, pH 7 dan konsentrasi urea 16,6 mM dengan nilai aktivitas 407,62 U/mL. EDTA dan ion logam dalam CaCl2, NaCl, NiCl2 dan CuCl2 pada variasi konsentrasi 10-3, 10-4  dan 10-5 M merupakan inhibitor urease FA 20 dari kacang panjang.Partial Purification and Characterization of Urease from Asparagus Bean (Vigna unguiculata subsp sesquipedalis L.). Urease is an enzyme used in urea hydrolysis to ammonia and bicarbonate acid and has been widely used in industrial processes. The study focused on isolation and purification of urease from asparagus beans and its characterization. The study was started with germination of asparagus beans for 8 days. Germinated asparagus beans were further extracted using phosphate buffer pH 7 and separated by centrifugation to obtain a crude extract of urease. The crude extract of urease was further fractionated using acetone at concentrations of 20, 40, 60 and 80%. The fraction with highest specific activity was then analyzed using SDS-PAGE method to determine its molecule weight and characterized further including the influence of temperature, pH, substrate concentration, and metal ion addition to urease activity. The urease activity was determined by the Nessler̕ s method. The results showed that the specific activity of urease from asparagus beans was found with highest activity in fraction of acetone (FA) 20. Analytical result using SDS-PAGE method was obtained some protein bands having molecular weights about 25 KD and 17 KDa. The optimum conditions of urease activity was obtained at 30 °C, pH 7, incubation time 20 min and urea concentration 16.6 mM with activity value 407.62 U/mL. EDTA and metal ions contained in CaCl2, NaCl, NiCl2 and CuCl2 at concentrations of 10-3, 10-4 and 10-5 M were FA 20 urease inhibitors.
The Characteristics of Urease Enzyme of Green Bean Seeds (Vigna radiata L.) and Its Activity as An Antifungal Against Candida albicans Niken Istikhari Muslihah; Zusfahair Zusfahair; Dian Riana Ningsih; Fajar Nuradha
Jurnal Kimia Sains dan Aplikasi Vol 27, No 3 (2024): Volume 27 Issue 3 Year 2024
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.27.3.145-150

Abstract

Urease is an enzyme responsible for catalyzing the hydrolysis reaction of urea into CO2 and NH3. The urease isolated in this study came from green bean seeds. The urease enzyme was then tested for its antifungal activity against Candida albicans. The research aims to extract and characterize the urease enzyme from green bean seeds and explore its potential use as an antifungal agent. Green bean seeds were smoothed with a mortar and pestle, followed by homogenization using a stirrer and cold centrifugation. The crude extract of the urease enzyme was assessed for its activity through the Nessler method and measured by employing a UV-Vis spectrophotometer at a wavelength of 500 nm. The well-diffusion method was conducted to determine the antifungal activity of rough enzyme extracts against C. albicans. Positive controls were 100% ketoconazole, and negative controls used a pH buffer of 7. The characterization of the urease enzyme from green bean seeds revealed the optimum urease activity at a concentration of 0.25 M, pH level of 7, and an incubation temperature of 35°C with a value of 32.115 U/mL. Maximum reaction rate (Vmax) and Michaelis-Menten constant value (KM) were obtained at 56.497 U/mL and 0.215 M, respectively. Antifungal tests of C. albicans resulted in strong inhibitory activity at a concentration of 100% crude urease extract of 12 mm inhibition zone. The inhibitory concentration value grows at least 0.5% by 0.25 mm and positive control of 19.802 mm.
Co-Authors A.A. Ketut Agung Cahyawan W Ahmad Nurdin AMIN FATONI Anung Riapanitra Apriliadina, Inessa Putri Ari Asnani Aryanti, Eva Bilalodin Bilalodin Dadan Hermawan Darul Santri Pertiwi Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih DIAN RIANA NINGSIH Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Diyu Mantari Dwi Kartika Elok Dwi Putri Lestari ELY SETIAWAN Fajar Nuradha Febrina Nur Habibah Indah Permatawati Irmanto Irmanto Istinganatun Khoeriyah Luthfia, Adilla Mardiyah Kurniasih Mardiyah Kurniasih Mei Lianasari Mekar Dwi Anggraeni Muslihah, Niken Istikhari Niken Istikhari Muslihah Nuraini, Aprilia Nafi Puji Lestari PUJI LESTARI Puji Lestari Puji Lestari Purwati Purwati Purwati Purwati Purwati Purwati Purwati Purwati Purwati Purwati Senny Widyaningsih Senny Widyaningsih Senny Widyaningsih Setiawan, Ely Sulistyowati, Aris Suyata Suyata Tien Setyaningtyas Uyi Sulaeman Vika Aprilia Puspitarini