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Decreasing SGPT level and macrophage activity through CD68 expression in the Balb/c mice (Mus musculus) liver infected with Salmonella typhi after treating with atung seeds (Parinarium glaberimmun Hassk) Eifan Boyke Pattiasina; Pieter Kakisina; Ferymon Mahulette
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 1 No 1 (2021): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (628.827 KB) | DOI: 10.30598/bioedupat.v1.i1.pp17-23

Abstract

Normally macrophages are always in the body and spread in various body tissues such as liver tissue (Kupffer cells). Macrophages in tissue can be identified by the expression of several markers, in humans the marker is CD68. The increase and decrease in macrophage activity in the liver can also be indicated by an increase in SGPT levels so that atung seeds have the ability to inhibit the growth of S. tyhpi bacteria which contain tannin compounds which can damage microbial cell walls and form bonds with microbial functional proteins so that bacterial growth is inhibited. The purpose of this study was to determine the SGPT levels and macrophage activity. The method used is laboratory experiment. The results showed an increase in SGPT levels in the positive control (87.00 ± 2,915) and a concentration of 25% (84.20 ± 3,962) and a decrease in SGPT levels in the negative control (50.80 ± 2.168 *), 50% concentration (78.20 ± 3.114 *) and concentrations. 75% (58.20 ± 3,834), decreased macrophage activity in the liver also occurred at a concentration of 50% and at a concentration of 75%, the liver was normal, which was indicated by the resulting brown expression
Effectiveness of Chinese Ketepeng Infusion on Streptozotocin-Induced Mice Watuguly, Theopilus Wilhelmus; Pattiasina, Eifan Boyke
Jurnal Kesehatan Masyarakat Vol 19, No 2 (2023)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v19i2.42825

Abstract

Blood sugar is a metabolic disorder disease that occurs due to insufficiency of insulin hormone production or due to insulin insensitivity resulting in typical clinical manifestations in the form of increased glucose levels in the blood (hyperglycemia). This study aims to determine the effect of Chinese ketepeng leaf decoction on blood glucose levels in hyperglycemic mice. This research was carried out in the Zoology laboratory, Faculty of Mathematics and Natural Sciences, Pattimura University, Ambon. This research is an experimental laboratory with a pre and post-test research design with a control group design. This study wanted to prove whether there was an effect of giving Chinese ketepeng leaf decoction to decreasing blood sugar levels in male Balb/C mice using Chinese ketepeng leaf decoction at a dose of 3.64 mg/kg, 7.28 mg/kg, 10.92 mg/kg. The results showed that the usage of Chinese ketepeng leaves (Cassia alata L) can reduce blood sugar levels in mice (Mus musculus). The most effective dose to lower blood sugar levels is a dose of 10.92 mg/kg BW.
ALIGNMENT DAN ANALISIS KEKERABATAN RAYAP COPTOTERMES FORMOSANUS (Isoptera: Rhinotermitidae) PADA HABITAT YANG BERBEDA DENGAN SOFTWARE BIOEDIT DAN MEGA 6 (STUDI PUSTAKA DNA SEKUENSING DARI NCBI) Leiwakabessy, Fredy; Awan, Ali; Kubangun, Muhammad Tarmizi; Pattiasina, Eifan Boyke
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 10 No 1 (2023): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol10issue1page01-10

Abstract

Background: Bioinformatics is a science that can relate information which includes molecular biology, biochemical structure, enzymatic, cell biology, physiology and pathology by using a computerized system based on data that has been collected. Methods: sequence alignment and phylogenetic tree construction of DNA sequences registered at NCBI in the subterranean termite Coptotermes formosanus Shiraki that lives in different habitats, namely in Mississippi, Southeast Asia, and China using Bioedit and MEGA software. Results: Sequence alignment showed that termite C. formosanus isolated from MS12 gene COII (FJ870577.1) in the Mississippi area had a base pair of 790 bp, termite C. formosanus isolated from Dinghu Mountain gene COII (KU257993.1) in China has a base pair of 779 bp, and the termite C. formosanus isolated from CF003JP gene COII (EF379943.1) in Southeast Asia has a base pair of 676 bp. The construction of the phylogenetic tree shows that the termite C. formosanus isolated from Dinghu Mountain gene COII (KU257993.1) in the China region has a close kinship with the termite C. formosanus isolated from CF003JP gene COII (EF379943.1) in the Southeast Asia region, while The termite C. formosanus isolated from MS12 gene COII (FJ870577.1) in the Mississippi area is distantly related to C. formosanus (KU257993.1) from China and C. formosanus (EF379943.1) from Southeast Asia. Conclusion: Mississippi has a base pair of 790 bp, termite C. formosanus isolated from Dinghu Mountain gene COII (KU257993.1), China has a base pair of 779 bp, and termite C. formosanus isolated from CF003JP gene COII (EF379943.1), Southeast Asia has a base pair of 676 bp. China is closely related to the termite C. formosanus isolated from COII gene CF003JP (EF379943.1), Southeast Asia, while the termite C. formosanus isolated from MS12 gene COII (FJ870577.1), Mississippi is distantly related to C. formosanus (KU257993.1) from China and C. formosanus (EF379943.1) from Southeast Asia.
PROFIL STRUKTUR KOMUNITAS LAMUN DI PERAIRAN PANTAI KELAPA INDAH DESA MAHU KECAMATAN SAPARUA TIMUR SEBAGAI SUMBER PEMBELAJARAN BIOLOGI Tuapattinaya, Prelly; Pattiasina, Eifan Boyke; Pattipeilohy, Johana; Sahetapy, Marcelino; Wattimena, Chilf
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 10 No 1 (2023): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol10issue1page148-155

Abstract

Background: Seagrass is a higher plant (Anthophyta) that lives and grows immersed in the marine environment; have vessels, have rhizomes, have roots, and reproduce generatively (seeds) and vegetatively. Seagrass is one of the natural resource ecosystem plants that lives in shallow waters and has many benefits for biota associated with the surrounding environment. Several studies related to seagrass ecosystems have been carried out in Maluku waters, but there are no records regarding the structural profile of seagrass communities on Saparua Island. Methods: This research is descriptive research. Descriptive research is used to reveal information about hydrological conditions, species composition, species diversity. This research was conducted in the waters of Kelapa Indah Beach, Mahu Village, Saparua District Results: The conditions of environmental physical and chemical factors with a temperature of 280C, salinity 31 ‰, pH 7.3, and Dissolved Oxygen 6 mg/L in the waters of Mahu village are sufficient to support the growth and spread of seagrass. Composition of the types of seagrass found in the coastal waters of Mahu village are 5 types and Conclusion: The level of seagrass diversity in Mahu village waters is classified as moderate.
IDENTIFICATION OF MEDICINAL PLANTS AND BENEFITS IN THE VILLAGE OF NEGERI LIMA, MALUKU DISTRICT Nusaly, Windy Natalia; Watuguly, Theopilus Wilhelmus; Pattiasina, Eifan Boyke; Wael, Syahran
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 10 No 1 (2023): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol10issue1page156-170

Abstract

Backgraound: Medicinal plants are the biodiversity that exists around us, both those that grow wild and those that are deliberately cultivated. For generations, plants have been used as medicinal plants. The largest part of medicinal raw materials derived from plants is still not cultivated, while opportunities for agribusiness have the potential to increase the opportunities and possibilities open for medicinal products. Methods: The aim of the research is to find out the types of medicinal plants and find out how the community perceives the benefits of medicinal plants in the village of Negeri Lima. Maluku. The material used in this research is a questionnaire or questionnaire. The research uses the Guttman scale by distributing questionnaires to respondents which are used to find scores, percent scores, total scores, and percent ideal scores, the results of which are converted into score interpretation criteria. Results: The research results showed that the criteria distributed to respondents were very good with the highest total score being 90% and the lowest being 10%. Conclusion: As a result of the identification carried out, 20 types of medicinal plants were identified which were spread across Negeri Lima Village, Central Maluku. Analysis of questionnaires distributed to the public to measure public perceptions by distributing questionnaires used the Guttman scale from question no. 1 - 5, it is known that the highest percentage is 90% and the lowest percentage is 10%. Meanwhile, the results of the analysis of attitude question items to measure public perception from question no. 6 - 10 has the highest percentage, namely 80% and the lowest percentage, namely 20%.
Effect of Drying Duration on Water Content and Ash Content of Red Salak Fruit (Salacca edulis) Herbal Tea Smith, Alwi; Pattiasina, Eifan Boyke; Simal, Rufiati; Karepesina, Fitriah Husna; Raharusun, Ayu Ningsih; Baysi, Asmawati Rahma
Journal of Tropical Agricultural Engineering and Biosystems - Jurnal Keteknikan Pertanian Tropis dan Biosistem Vol. 12 No. 3 (2024): December 2024
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkptb.2024.012.03.02

Abstract

Negeri Riring, Taniwel District, West Seram Regency, Maluku Province, has many agricultural opportunities for red salak. Salak is unique with thick, dark yellow flesh with a red tinge, which is why it is called red snake fruit. Currently, the red salak plant is only used for its fruit flesh; the skin and other parts of the plant are immediately removed and disposed of in the environment. Red salak fruit skin, which has high antioxidant and polyphenol content, can be utilized and processed into new products, such as herbal tea. Drying is an important stage in the process of making herbal tea. Making herbal tea from red salak fruit skin is carried out in the Biology Laboratory. The aim of this research is to determine how long drying impacts water and ash content. The research method used was an experiment using a simple Completely Randomized Design (CRD), namely at the same temperature, namely 60°C and with a long drying treatment. The Ambon Industrial Standardization and Services Center tests water and ash content. Analysis of variance (ANOVA) was used to analyze research data at a confidence level of 5%. The results showed that the drying time had a significant impact on the water content, but did not have a significant impact on the ash content of the red salak fruit rind herbal tea.
INVENTARISASI KEANEKARAGAMAN TUMBUHAN LUMUT (Bryophyta) PADA HABITAT ALIRAN SUNGAI DAN HUTAN WAITATIRI, AMBON Pradana, Sendy Putra; Tuapattinaya, Prelly Marsell Jolanda; Latupeirissa, Louvenska Nona; Pattiasina, Eifan Boyke
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 2 (2025): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue2page259-265

Abstract

Background: Bryophytes (Bryophyta) are small terrestrial plants that are generally green in color. This study aims to identify the types of bryophytes (Bryophyta) and classify their presence on various substrates around the Waitatiri River and forest, Ambon. Methods: The study was conducted along the Waitatiri River, Ambon, using the exploratory survey method. Data were collected through direct observation of bryophyte species in the field. Results: The results revealed various types of bryophytes, including liverworts (Marchantiophyta) and mosses (Bryopsida). Identified species included Rhizomnium punctatum, Dicranum scoparium, Spahgnum fimbriatum, Polytrichum juniperinum, Marchantia polymorpha, and Metzgeria furcata.. The highest distribution was found on rocky substrates with two dominant species, while the lowest distribution was observed on tree bark. Conclusion: This study highlights the variation in Bryophyta species distributed on rocky substrates and tree bark within the Waitatiri ecosystem, Ambon, with the largest species distribution found on rocky substrates.
Processing and Utilization of Sago palm in Central Moluccas Mahulette, Ferymon; Matulessy, Yoin M; Pattiasina, Eifan B; Rupilu, Marselina R
Local Wisdom Jurnal Ilmiah Kajian Kearifan Lokal Vol. 13 No. 1 (2021): January 2021
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/lw.v13i1.4406

Abstract

Indigenous people in Moluccas consumed sago flour as a main food. It is extracted from the pith of the sago palm (Metroxylon sp) by traditional and modern processing. Sago flour can be processed into various local food products which are local wisdom of the Moluccan. These products are produced by a very diverse community. In addition, stalks and leaves of sago tree can also be used for building construction. This study aims to describe the processing techniques and utilization of sago palm in Central Moluccas. This research was descriptive qualitative. Data was collected from direct observations and documentation from volunteers in several villages only in Central Moluccas, i.e. Hila village (Ambon), Tuhaha and Ouw villages (Saparua), Kamarian and Iha-Luhu villages (West Ceram), and several villages in Geser and Gorom islands (East Ceram). The results showed that sago processing in Central Moluccas was done manually (traditional) or machinery. The processing technique these plants was relatively the same but the processed sago products vary greatly in each village. Sago processed products such as papeda, sagu lempeng, sagu tumbuk, serut, sagu buksona, sagu lakar and sagu fitrah. Besides it flour used as food, stalks and leaves of sago tree are used to build traditional houses, baileo and worship houses. This research was expected to conserve of the pukul sagu tradition and sago processed products to support the tourism in Central Moluccas DOI: https://doi.org/10.26905/lw.v13i1.4406
Analysis of Water Suitability for Ecotourism Development on Several Beaches in Morella Village, Central Maluku Regency, Maluku Province Leiwakabessy, Fredy; Kubangun, Muhammad Tarmizi; Pattiasina, Eifan Boyke
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 17 No. 2 (2025): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v17i2.63710

Abstract

Maluku is an archipelago whose territory is coastal and marine which is suitable for tourism. The tourism value and attractiveness of coastal areas should be developed through marine tourism. This study aims to analyze current speed, water depth, pH, DO, temperature, salinity, sigma-t, chlorophyll-a, and turbidity-m as physical, chemical, and biological indicators in the Lubang Buaya and Moki Beach ecotourism areas. Water sampling was carried out using Kemmerer bottles at different depths, namely at the surface and at a depth of 20 meters.Water samples are used to analyze chemical elements both horizontally and vertically. Sample analysis was carried out at the Oceanology Laboratory and Basic Biology Laboratory of Pattimura University.The test parameters in this study were water depth, temperature, salinity, sigma-t, chlorophyll-a, turbidity-m using conductivity, temperature, and depth (CTD), current speed using a current meter, pH using a pH meter, and dissolved oxygen (DO) using a DO meter. The waters of Lubang Buaya Beach and Moki Beach, Morella Village, Central Maluku Regency, Maluku Province have water conditions, namely pH, temperature, salinity, sigma-t, chlorophyll-a, turbidity-m, and brightness with appropriate categories, while DO with an inappropriate category based on water assessment standards. The assessment results show the waters' suitability for ecotourism development at a surface depth of 0 meters and a bottom depth of 3 to 25 meters. The minimum current speed of 0.003 m/s and the maximum current speed of 0.36 m/s are suitable for boating, banana boating and jet skiing activities.
The Role of Public Service Centers in Shaping Community Waste Management Behavior in Ambon City Alwi Smith; Ayu Christine Rahaweman; Eifan Boyke Pattiasina
Publisia: Jurnal Ilmu Administrasi Publik Vol. 11 No. 1: April 2026
Publisher : Universitas Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/pjiap.v11i1.15917

Abstract

Generally, the waste management has to involve the entire society component, not only the government but also the society itself. The government can be useful with their regulation and their productive programs related to waste management. But the regulation and the program can only be successfully applied if the society actively takes part in the grand plan designed by the government. In this paper, we analyzed the influence between the waste facility provide by the government, self-knowledge of the society about waste management and society figure who can be a role model in managing the waste to the society behavior of waste self-management in Ambon Moluccas. This research showed that only 23.83% of the society behavior of waste self-management in Ambon Moluccas can be analyzed through the variables of waste facility, self-knowledge and society figure simultaneously. Furthermore, it is also shown us that the Society behavior variable can be explained 17.53% by the waste facility variable, 14.19% by the self-knowledge and 1.63% by the society figure particularly.