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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Veteriner Microbiology Indonesia Indonesian Journal of Tropical and Infectious Disease UNEJ e-Proceeding Dental Journal (Majalah Kedokteran Gigi) Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Respirologi Indonesia J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Respirasi (JR) JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Abdi Insani Jurnal Layanan Masyarakat (Journal of Public Service) Indian Journal of Forensic Medicine & Toxicology Berkala Penelitian Hayati JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Journal of Halal Science and Research Journal of Clinical Microbiology and Infectious Diseases Jurnal Widya Medika Folia Medica Indonesiana
Ni Made Mertaniasih
Departement Of Medical Microbiology, Faculty Of Medicine Universitas Airlangga, Dr. Soetomo General Hospital Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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THE ANTI-TB DRUG SENSITIVITY OF Mycobacterium tuberculosis FROM CEREBROSPINAL FLUID AND BONE TISSUE BIOPSY SPECIMENS OF PATIENTS SUSPECTED TUBERCULOUS MENINGITIS AND SPINAL TB IN Dr SOETOMO HOSPITAL INDONESIA Mertaniasih, Ni Made; Kusumaningrum, Deby; Koendhori, Eko Budi; Harijono, Sugeng; Arky, Catur Endra; Putri, Jayanti; Urifah, Hanik
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 3 (2014)
Publisher : Institute of Topical Disease

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Abstract

Tuberculous meningitis (TBM) is an infection of meningens which potentially life threatening with significant morbidity and mortality. Spinal TB has the same problem with TBM, infection in bone and joint, the delayed diagnosis worsens the prognosis. The rapid and accurate diagnosis plus promt adequate treatment is essential for the good outcome. The aim of this research is to study thefirst line drug sensitivity of Mycobacterium tuberculosis isolated from specimens of cerebrospinal fluid from suspected tuberculous meningitis patients and bone tissue biopsy from suspected spinal TB patients. The method of this research is TB Laboratory examination in Department of Clinical Microbiology – Dr. Soetomo General Hospital, Indonesia, using the gold standard liquid culture method MGIT 960 System (Becton Dickinson) and solid culture method with Lowenstein-Jensen medium. The specimens CSF from 50 TBM patients at January 2013 until May 2014. Positive isolate detection of Mycobacterium tuberculosis complex were 11 isolates (22%), which sensitivity 100% (11/11 isolates) to Rifampin (R), Pyrazinamide (Z), Ethambutol (E), and Streptomycin (S); one isolate resistant to Isoniazid, sensitivity to Isoniazid 90,90% (10/11); and received 21 specimens of bone tissue biopsy which positive 5 isolates(23%), all isolates sensitive 100% (5/5 isolates) to Rifampin and Pyrazinamide, and 1 isolates resistant to Isoniazid, Ethambutol, and Streptomycin, in which sensitivity 80% (4/5 isolates) to Isoniazid, Ethambutol, and Streptomycin. The conclusion of this research is positivity detection 22% of CSF specimens, and 23% of bone tissue biopsy were low. All isolates sensitive 100% to Rifampin and Pyrazinamide, and 80-90% sensitive to Isoniazid.
CELLULAR IMMUNITY ACTIVATION METHOD BY STIMULATING RD1 COMPLEX PROTEINS AS VIRULENCE MARKER ON Mycobacterium tuberculum TO ESTABLISH DIAGNOSIS ON TUBERCULOSIS AND LATENT TUBERCULOSIS INFECTION Setiabudi, Rebekah; Mertaniasih, Ni Made; Didik Handijatmo, Didik; Setyoningrum, Retno Asih
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 7 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v5i7.1205

Abstract

This study was intended to invent a simpler and more affordable method to establish diagnosis on Tuberculosis (TB) and Latent Tuberculosis infection (LTBI). Similar to “Quantiferon TB Gold In Tube” (QFT-GIT) and T.SPOT.TB methods, the researchers also utilized “early secreted antigenic target 6kDa” (ESAT-6) and “cultur filtrate protein 10kDa” (CFP-10) proteins to be induced on the specimen. ESAT-6 and CFP-10 are commercial products used to induce interferon gamma (INF-γ) which were to be read using sophisticated and expensive equipment. This study was intended to conduct an analysis on effective cocktail protein modification, i.e. ESAT-6, CFP-10 and Ag85A/B/C, with high validity to detect cellular immunity activity through in vitro examination on peripheral blood monocyte cells of Tuberculosis-suspected patients or patients with latent tuberculosis infection. Peripheral Blood Monocyte Cells (PBMCs) activity on children tuberculosis patient or Latent Tuberculosis Infection (LTBI), adult tuberculosis patient or LTBI, which induced by cocktail protein modification and not induced, were analyzed microscopically. The activity of PBMCs on children and adult tuberculosis patient or LTBI induced by RD1 secretory proteins: ESAT-6, CFP-10, Ag85A/B/C was higher compared to PBMCs which had not been induced by the secretory proteins. Cellular debris and monocyte cells with abnormal shapes were found on PBMCs which had been induced by RD1 secretory proteins at 8 th day after culture.
SUSCEPTIBILITY OF RIFAMPICIN-ISONIAZID RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATES AGAINST LEVOFLOXACIN Kurniawan, Alvin Hartanto; Mertaniasih, Ni Made; Soedarsono, S.
BALI MEDICAL JOURNAL Vol 5 No 1 (2016)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: Tuberculosis (TB) is a high burden disease in Indonesia with multidrug-resistant (MDR) TB incidence started to increase. Treatment success of MDR-TB globally was low in number than it was targeted which was especially caused by fluoroquinolone resistance. One of the fluoroquinolone is levofloxacin, an antibiotic that has been widely used irrationally as antimicrobial treatment. Therefore, this study investigated the sensitivity and MBC of MDR Mycobacterium tuberculosis isolates against Levofloxacin. Method: The susceptibility test for MDR -Mycobacterium tuberculosis on levofloxacin by standard method with levofloxacin were on concentrations 0,5 ?g/ml, 1 ?g/ml, and 2 ?g/ml. Sample of 8 strains MDR-Mycobacterium tuberculosis were cultured with each concentrations on Middlebrook 7H9 for 1 week incubation. Next, each of the incubated concentration was subcultured on solid media Middlebrook 7H10 for 3 weeks incubation. Colonized agar plates after 3 weeks incubation were confirmed with acid-fast stain. Results: On MB 7H10 with levofloxacin concentration 2 ?g/ml showed bactericidal effect 100% by no MDR Mycobacterium tuberculosis colony grew (0/8) while the MB 7H10 with levofloxacin concentration 1 ?g/ml and 0,5 ?g/ml showed the bactericidal effect 37,5% and 25% respectively. The colonized agar plate implied that the MDR Mycobacterium tuberculosis with levofloxacin concentration 1 ?g/ml (5/8) and 0,5 ?g/ml (6/8) grew well. Conclusion: Levofloxacin concentration 2 ?g/ml was susceptible on MDR Mycobacterium tuberculosis. The concentration 2 ?g/ml of levofloxacin could be considered as MBC.
SPECIFIC GYRB SEQUENCE OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATED FROM SPUTUM OF PULMONARY TUBERCULOSIS PATIENTS IN INDONESIA Mertaniasih, Ni Made; Wiqoyah, Nurul; Kusumaningrum, Deby; Soedarsono, S.; Perwitasari, Agnes Dwi Sis; Artama, Wayan Tunas
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: Indonesia have many different geographic areas which could be various on the variant strains of Mycobacterium tuberculosis. The gyrB gene codes GyrB protein as sub unit compound of Gyrase enzyme that functioning in multiplication of bacteria. Detection of gyrB gene could be a marker of active multiplication of viable bacteria in the specimen from patients; and some of the DNA sequence regions were conserved and specific in the strain of Mycobacterium tuberculosis that would be a marker for identification. This research aims to analyze the sequence of gyrB gene of Mycobacterium tuberculosis clinical isolates from sputum of pulmonary TB patients in Indonesia, and determine the specific region. Method: Mycobacterium tuberculosis clinical isolates have been collected from sputum of the patients with pulmonary TB that live in some area in Indonesia. Isolation and identification of Mycobacterium tuberculosis clinical isolates using standard culture method; sequence analysis using PCR-direct sequencing of the part bases region of gyrB. Results: this study revealed that nucleotide sequence on a fragment 764 bases of gyrB gene Mycobacterium tuberculosis strains among clinical isolates almost identically to a wild type strain Mycobacterium tuberculosis H37Rv and subspecies member of Mycobacterium tuberculosis complex (MTBC), with a little difference of SNPs; there are many difference nucleotide sequence with MOTT and Gram positive or negative bacteria, except Corynebacterium diphtheria identically with MTBC. Conclusion: the gyrB sequence in Mycobacterium tuberculosis strains among these clinical isolates from sputum of pulmonary TB patients in Indonesia have the conserved specific DNA region that almost identically with wild type strain H37Rv and MTBC.
Comparative Evaluation of Microscopy and Loop-Mediated Isothermal Amplification (Lamp) Assay for the Diagnosis of Tuberculosis Ni Njoman Juliasih, Rajesh Kumar Das2 , Ni Made Mertaniasih3 , Prabin Neupane4, , Reny Mareta Sari5
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10749

Abstract

Tuberculosis (TB) is one of the major global health problem which affects millions of people each year. For the routine diagnosis of TB, microscopic technique is used but it has lower sensitivity and specificity. This study was carried out to evaluate the efficacy of loop mediated isothermal amplification (LAMP) over smear microscopy to detect Mycobacterium tuberculosis. Out of 84 processed samples processed in this study, the diagnostic tests showed varying results. Using smear microscopy, 16(19.05%) and with the LAMP assay, 17(20.24%) cases were found positive for M. tuberculosis. The sensitivity of the LAMP assay (87.5%) was greater than that of microscopy (82.35%) while the specificity of both methods was comparable (>95%). Being more sensitive than microscopy, LAMP assay is more likely to show the positive result and solve the errors in diagnosis of the TB cases. Thus, LAMP assay can be an important and cost-effective tool for appropriate and timely diagnosis of TB patients. This can further assist to implement intervention programs against TB.
Modern Mycobacterium Tuberculosis Strain in Bronchoalveolar Lavage from Tuberculosis Patients Associated with Lung Tissue Damage Severity Budi Yanti1 , Muhammad Amin2 , Ni Made Mertaniasih2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11593

Abstract

Background: The Beijing sublineage modern Mycobacterium tuberculosis strain is the most dominantstrain in regards to causes of disease progression, extensive lung tissue damage, drug resistance and highoutbreak rates.Methods: Mycobacterium tuberculosis isolates were obtained from Bronchoalveolar lavage patients withactive pulmonary Tuberculosis before obtaining anti-tuberculosis drug treatments. The degree of severity ofparenchymal lung damage is classified by the NICE Scoring System. PCR was performed on DNA extractedfrom bronchial lavage, using primers targeting gene TbD1.Result: 30 active pulmonary tuberculosis patients were analyzed in this study. 13 isolates of modern strainsand 17 isolates of ancient strains were detected. In modern strains, 4 (30.8%) subjects had mild lung degreedamage, while 9 (69.2%) subjects had severe lung damage. In ancient strains, 12 (70.6%) subjects hadmild lung damage and 5 (29.4%) subjects had severe lung damage. Mycobacterium tuberculosis of modernstrains correlated with the degree of lung damage, p <0.05. Odds Ratio = 5.4 CI 95% (1,12-116,99).Conclusion: In Surabaya, modern strains of Mycobacterium tuberculosis were detected in BAL oftuberculosis patients. Radiograph evaluations revealed severe lung tissue damage. The risk of severe lungdamage with modern strains is 5.4 times higher than compared to ancient strains.
A Woman with Tuberculosis Multidrug Resistance and QTc Prolongation Repetitive Interval: A Case Report Tutik Kusmiati; Ni Made Mertaniasih; Johanes Nugroho Eko Putranto; Budi Suprapti; Soedarsono; Abdul Rahman Bahmid
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17119

Abstract

Background : Prolonged QTc interval is one of the side effects of the short-regimen MDR-TB. QTcprolongation is a risk factor for Torsades de pointes and is influenced by many factors. Case : 47-year-oldwoman who was diagnosed with MDR-TB through GeneXpert examination and received short-regimenMDR-TB. This patient experienced repeated QTc prolongation, with peak QTc interval 600 msec occurringat 4th month with mild hypokalemia without clinical symptoms. The patient completed 9 months of shortregimentherapy with improve chest x ray followed by negative sputum culture. Conclusion : Short-regimenMDR-TB contains several drugs that cause QTc prolongation. Clinical evaluation is required in patientswith QTc prolongation before changing the regiment.
Perbedaan Gambaran Histopatologi Granuloma Paru Mencit Setelah Diinfeksi Mycobacterium tuberculosis dan atau Intervensi Silika (THE INFLUENCES OF TIME IN THE HISTOPATHOLOGY OF LUNG GRANULOMA IN MICE AFTER INFECTION OF MYCOBACTERIUM TUBERCULOSIS AND SILI Ni Made Linawati; I Gusti Ngurah Mayun; I Gusti Nyoman Sri Wiryawan; Nyoman Sri Budayanti; Ni Made Mertaniasih; Fedik Abdul Ratam; I Nyoman Wande; I Gusti Ayu Dewi Ratnayanti; Ida Ayu Ika Wahyuniari; I Wayan Sugiritama; I Gusti Kamasan Arijana
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The characteristics of lung tuberculosis is granuloma, which is consisted of lymphocytes andmacrophages that show the interaction between immune cells and M.tb. Granuloma is the organizationprocess which is depend on lymphocytes invasion, adhesion molecules and chemokine fasilitation. Silicosiswhich is caused by silica, can influence granuloma in the lung. The features of granuloma is variationdepend on the elicited agent and immune reaction. The main purpose of this study was to prove thehistopathology differences of  mice lung granuloma caused by M.tb infection,  silica intervention and bothin 3th  and 7th weeks. It was 45 mice Balb-c strain, divided into 3 groups;  P1 got  M.tb infection with H37Rvstrain 105  perml,P2 got silica intervention with 60 micro litre and, P3 got both of M.tb infection and  silica intervention. Termination of each group were held on 3 and 7 weeks of intervention, continued byhistopathology examination. In the histopathology feature, we done semi-quantitative prosedure to measurelung damage by using Dormans scores; perivasculitis, peribronchiolitis, alveolitis and granuloma. Oneway anova to analysis the differences of histopathologycal result among these groups (P< 0,05).  Resultshowed the significant differences  among these group.  In the 3th weeks, we found  mild lung damage werehappened in all groups with granuloma, without necrosic (P1 and P2). In the 7th weeks we found  severe lungdamage in P3 with necrotic and fibrotic granuloma sign, with necrosis in P1, with fibrotic in P2.  Weconcluded the worst lung damage happened in 7th weeks in group which are got M.tb infection and silicaintervention, with granuloma characterictic of necrosic and fibrotic.
CLINICAL MANIFESTATION OF ORAL TUBERCULOSIS Atik Kurniawati; Ni Made Mertaniasih; Mangestuti Agil
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Tuberculosis (TB) is chronic infectious disease caused by Mycobacterium tuberculosis. According to WHO report, it infected almost one third people and Indonesia has been 5th position in the world. Tuberculosis is classified clinically as Pulmonary and Extra Pulmonary. Extra pulmonary tuberculosis can occur in the lymph nodes, meninges, kidneys, bone, skin and even oral cavity that we called oral tuberculosis. The oral tuberculosis may manifest in various form : ulcer, gingivitis, nodules, granulomatous, tuberculoma and osteomyelitis. The purpose of this paper to explain the clinical manifestation of oral tuberculosis.
The Relatedness between Hepatitis B Virus from Non-Papuan Blood Donors in Jayapura and the Papuan Clusters VICTOR EKA NUGRAHAPUTRA; MOCHAMAD AMIN; NI MADE MERTANIASIH; MARIA INGE LUSIDA
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.069 KB) | DOI: 10.5454/mi.3.2.7

Abstract

The genotypes (A-H) and subtypes (adw2, adw4, adrq-, adrq+, ayw1-4, ayr) of HBV show distinct geographical distributions, which have been associated with anthropological history. The novel finding of the HBV subgenotypes C6 and D6 from Papuans formed a specific cluster distinct from the previous HBV subgenotypes C1-C5 and D1-D5. In this study we determined the most recent genotype-subtype patterns of the HBV from non-Papuan blood donors who live in Jayapura and their phylogenetic relatedness, especially with the Papuan clusters. Fifteen HBsAg-positive serums were obtained from non-Papuan blood donors including from people in Java (46.7%), Maluku (26.7%), Sulawesi (20%) and East Nusa Tenggara (6.7%). S gene of all HBV serum isolates were partially sequenced and analyzed. Most HBV isolates (53.3%) were classified as genotype B, followed by genotype C(26.7%) and D (20.0%). The subtype adw2 (33.3%) was predominant, followed by adrq+ (26.7%) and ayw1/ayw2 (20.0%). All HBV isolates with subtype adw2 and ayw1 belonged to genotype B, while adrq+ belonged to genotype C and ayw2 belonged to genotype D. The most predominant HBV genotype-subtype (B/adw2) was consistent with the ethnic background (mostly from Java people). Nevertheless, based on the phylogenetic relatedness, many non Papuan isolates (40%) were classified into HBV/C6 and HBV/D6 of the Papuan clusters. Other isolates were classified into HBV/C1, HBV/B3 and HBV/B7. In conclusion, many HBV isolates from non-Papuans in Jayapura belonged to the Papuan clusters, but others had different genotype-subtype patterns with frequencies dependent on ethnicity.
Co-Authors , Atika Abdul Rahman Bahmid Agnes Dwi Sis Perwitasari, Agnes Dwi Sis Aisah, Nurul Akirasena, Mayoori Alimsardjono, Lindawati Alvin Hartanto Kurniawan, Alvin Hartanto Ananda, I Gede Yogi Prema Andy Setiawan Asri Darmawati Atik Kurniawati Atika Ayu Lidya Paramita Ayu Lidya Paramitha Bagus Soebadi Budi Suprapti Budi Yanti Budi Yanti Catur Endra Arky, Catur Endra Deby Kusumaningrum Diah Savitri Ernawati Didik Didik Handijatmo, Didik Dimas Firman Hidayat Eko Budi Koendhori, Eko Budi Endraswari, Pepy Fedik Abdul Ratam Hanik Urifah, Hanik Hidayat, Dimas Firman I Gusti Ayu Dewi Ratnayanti I Gusti Kamasan Arijana I Gusti Ngurah Mayun I Gusti Nyoman Sri Wiryawan I Nyoman Wande I Wayan Agus Gede Manik Saputra I Wayan Sugiritama Idha Kusumawati Ikhwani, Irfan Arif Irfan Arif Ikhwani Irwanto Irwanto Isnaeni Isnaeni Isnaeni Isnin Anang Marhana Jayanti Putri, Jayanti Juita, Liza Puspa Senja Asmara Junus, Herisa Nataliana Kadariswantiningsih, Ika Kawilarang, Arthur Pohan Kusmiati, Tutik Kusmiati, Tutik Lestari, Aprilia Dwi Lyndia Effendy Maharani, Sekar Mahdani, Wilda Mangestuti Agil ManikRetno Wahyunitisari Maria Inge Lusida Marsha Maritsa, Olivia Mega Ferdina Suwito Mochamad Amin Mochammad Afif Ziaulhaq Muhammad Amin Muhammad Amin Mulyadi Mulyadi Ni Made Linawati Ni Nengah Dwi Fatmawati Ni Nyoman Sri Budayanti Nurrosyidah, Iif Hanifa Nurul Wiqoyah, Nurul Nuswantoro, Djohar Paramitha, Ayu Lidya Pepy Dwi Endraswari, Pepy Dwi Permatasari, Ariani Priyo Hadi Purwanta, Marijam Putranto, J.Nugroho Eko Raharjo, Dadik Rarome, Berlian Beatrix Ratna Kusumawati Ratna Kusumawati Rebekah Setiabudi, Rebekah Reiska Kumala Bakti Resti Yudhawati Retno Asih Setyoningrum Risa Etika, Risa S. Soedarsono Sakran, khawla Abdullah Salsabila, Sabila Sampurna, Mahendra Tri Arif Saputra, I Wayan Agus Gede Manik Sari, Trias Kusuma Sekar Maharani Setiawan, Firman Silvia Sutandhio Soedarsono Soedarsono SOETJIPTO . Soetjipto Soetjipto Sugeng Harijono, Sugeng Sulistyowati, Titiek Titiek sulistyowati Titiek Sulistyowati Titiek Sulistyowati VICTOR EKA NUGRAHAPUTRA Wayan Tunas Artama Widya, Alicia Margaretta Wilda Mahdani Wiwin Retnowati Yelvi Levani Yitijuatni Yudayanti, Elprania Credo