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All Journal HAYATI Journal of Biosciences SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Microbiology Indonesia Biotropika Science and Technology Indonesia Manuju : Malahayati Nursing Journal Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Biomedika dan Kesehatan East Asian Journal of Multidisciplinary Research (EAJMR) Jurnal Jaminan Kesehatan Nasional Makara Journal of Science Indonesian Journal of Biomedicine and Clinical Sciences
SJATHA, FITHRIYAH
Departement Of Microbiology, Faculty Of Medicine, Universitas Indonesia, Jakarta, DKI Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Journal : Microbiology Indonesia

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Levels of TNF-α in PBMC (Peripheral Blood Mononuclear Cells) Induced by Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in vitro FITHRIYAH SJATHA; OKTIVIA CHANDRA MUSTIKA; BETI ERNAWATI DEWI; TJAHJANI MIRAWATI SUDIRO
Microbiology Indonesia Vol. 13 No. 2 (2019): June 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (709.517 KB) | DOI: 10.5454/mi.13.2.4

Abstract

Dengue infection is a global health problem with an increasing incidence every year and now endemic in more than 100 WHO countries. Dengue infection is caused by dengue virus (DENV) which is an RNA virus with positive single strand, with ±11kb genome size encoding 3 structural proteins, 7 non-structural proteins, and two Untranslated Region (UTR). NS1 protein is known to have a very important role in the development of severe DENV infection, by the direct effect causing host cells damage and indirect effect by activating immune response to induce the secretion of excess cytokines. This study aims to evaluate whether recombinant pcNS1 plasmids which have been proven expressing recombinant NS1 proteins in previous studies is able to induce cytokine secretion from Peripheral Blood Mononuclear Cells (PBMC). Transfected Chinese Hamster Ovary-K1 (CHO-K1) cells with recombinant pcNS1 plasmid was co-cultured with PBMC from healthy donor. After 48 h post co-cultured, cell supernatant was collected and TNF-α levels and NS1 recombinant were measured by ELISA. The results showed that recombinant NS1 protein was expressed in CHO-K1 mammalian cell line and able to induce TNF-α with higher levels compared to control.
The qPCR Assay for Detecting The Presence and Relative Abundance of Pseudomonas aerugionosa and Antibiotic Resistance Gene aadA2 in Hospital Wastewater of National Reference Hospital Dr. Cipto Mangunkusumo (RSCM) Rida Tiffarent; Rosdiana Irawati; Conny Riana Tjampakasari; Fithriyah Sjatha; Windi Muziasari; Anis Karuniawati
Microbiology Indonesia Vol. 16 No. 2 (2022): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.977 KB) | DOI: 10.5454/mi.16.2.24-30

Abstract

Antimicrobial resistance is one of the top 10 global health threats. The hospital wastewater (HWW) potentially becomes the reservoir and dissemination of antibiotic resistance gene (ARG) and bacterial pathogens. In Indonesia, the protocol to monitor the ARGs form HWW has not been established. This study aimed to detect the presence and find the relative abundance of P. aeruginosa and aadA2 genes from Dr. RSUPN. Cipto Mangungkusumo (RSCM) inlet and outlet wastewater through qPCR assay. The primers used were supported by Resistomap. The study revealed that the qPCR assay was able to detect the Ct value of P. aeruginosa and aadA2. The aadA2 gene was found in all waste water samples, meanwhile P. aeruginosa was only found in some of inlet samples. aadA2 had the highest relative abundance and this gene’s mobility uses plasmids and integrons that potentially enhance the acquired antimicrobial resistance (AMR) mechanism. This study implicated that qPCR assay was capable to detect pathogenic bacteria and ARG, and ARG could be released to the environment even though the wastewater samples have been proceeded in wastewater treatment plants (WWTP). The qPCR assay can be used as the method to monitor the AMR status in a hospital and the spreading potency to the environment using the HWW.
Co-Authors . Andriansjah Abas, Ghina Mutiara Adiyaksa, Jongga Andi Yasmon Anis Karuniawati Aya Yuriestia Arifin, Aya Yuriestia Beti Ernawati Dewi Clara Riski Amanda Engel, Yulia Fadilah Fadilah, Fadilah Hanifah Oswari Hansur, Lismayana Mardhiyah, Azzahrah Khairunnisa Marintan, Marchia Marthalena Mifa Nurfadilah Muhamad Rizqy Fadhillah Nur Azizah Nur Azizah Nurani, Dita Arifa Nurul Gusti Khatimah OKTIVIA CHANDRA MUSTIKA Purnomosidi, Muhammad Abhi Rida Tiffarent Rizky Clarinta Putri Rosa, Yulia Rosdiana Irawati Safrullah, Muhamad Iqbal Sariman Sariman Supardi, Lulut Azmi Tjahjani Mirawati Sudiro Tjampakasari, Conny Riana Windi Muziasari Yeva Rosana Yulia Rosa Saharman Yuni Krisyuningsih Krisnandi, Yuni Krisyuningsih Zain, Paisal