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Evaluasi Penggunaan Biosaliva Dalam Deteksi Sars-Cov-2 Metode RT-PCR Suryanata Kesuma; Suparno Putera Makkadafi
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.11280

Abstract

SARS CoV-2 infection, which has affected the world since late 2019, can cause serious lower respiratory tract infections that may be fatal in some patients. This infection causes the disease Covid-19. The diagnosis of SARS-CoV-2 infection was carried out by NAAT (Nucleic Acid Amplification Test) such as RT-PCR examination. The sample needed for the identification of SARS-COV-2 is a nasopharyngeal/oropharyngeal swab. Nasopharyngeal/oropharyngeal swab sampling requires trained personnel. Taking a nasopharyngeal/oropharyngeal swab is invasive, causing discomfort in its implementation. The convenience of sampling specimens can be an alternative option for the identification of SARS-CoV-2, such as with newly developed biosaliva specimens. The use of this biosaliva sample can be a practical option in the examination of the identification of SARS-CoV-2. However, the use of these specimens needs to be evaluated first because of the possible relationship with clinical findings and so that the results of the SARS-CoV-2 examination are valid and reliable. The purpose of this study was to evaluate the use of biosaliva specimens to detect SARS-CoV-2 infection with the RT-PCR method. Evaluation of the use of biosaliva in the detection of SARS-COV-2 RT-PCR method with paired T test and diagnostic test with the gold standard using nasopharyngeal/oropharyngeal swabs. The target genes for the detection of SARS-CoV-2 are the RdRp gene and the E gene with control of the HRP gene. RT-PCR was carried out with 40 cycles and Tm 62 °C. The results of this study are Sig. (2-tailed) paired T test was 0.106, sensitivity was 64.86% and specificity was 90.92%. The conclusion of this study is that there is no statistical difference in the results of the SARS-CoV-2 RT-PCR method between the use of biosaliva specimens and nasopharyngeal/oropharyngeal swabs, and the evaluation results show that reliable biosaliva specimens are used as samples in the examination of SARS-COV-2 infection.
IDENTIFIKASI BAKTERI PATOGEN PADA MINUMAN SUSU FORMULA 2 JAM SETELAH DISEDUH Rizqi Fakhrial Husain; Suparno Putera Makkadafi; Ganea Qorry Aina
Jambura Journal of Health Sciences and Research Vol 5, No 3 (2023): JULI: JAMBURA JOURNAL OF HEALTH SCIENCES AND RESEARCH
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/jjhsr.v5i3.20478

Abstract

Susu formula adalah susu yang terbuat dari susu sapi atau kedelai yang telah dimodifikasi. Pemberian susu formula bayi harus disesuaikan dengan umur, kondisi bayi, dan sesuai dengan takaran saji. Menurut World Health Organization (WHO) atau Food and Agriculture Organization (FAO) pada tahun 2007, bubuk susu formula bayi tidak steril. Bahan tersebut mengandung bakteri yang dapat menyebabkan penyakit serius pada bayi. Hal ini didukung penemuan dari badan POM Amerika yang menemukan 14% susu formula terkontaminasi Enterobacter sakazakii (Cronobacter spp.). Bakteri pada susu mampu memperbanyak diri setiap 20 menit maka dianjurkan jangan mengkonsumsi susu yang sudah disiapkan lebih dari 2 jam. Kebaruan peneitian ini karena meneliti tentang bakteri pathogen pada minuman susu formula 2 jam setelah diseduh. Tujuan penelitian ini adalah untuk mengetahui cemaran bakteri patogen pada minuman susu formula 2 jam setelah diseduh. Penelitian ini adalah penelitian deskriptif. Jumlah sampel sebanyak 5 sampel susu formula 2 jam setelah diseduh. Data dianalisa dengan univariat yaitu dengan pengamatan secara makroskopis dan mikroskopis lalu dihitung menggunakan rumus persentase dengan rumus frekuensi jumlah sampel yang positif bakteri patogen dibagi jumlah sampel. Hasil dari penelitian ini ditemukan adanya bakteri patogen dengan persentase 100% yang terdiri dari jenis Bacillus cereus dengan persentase 60% dan Bacillus licheniformis dengan persentase 40%. Kesimpulan penelitian ini adalah ditemukan adanya pertumbuhan bakteri patogen (100%) dari 5 sampel yang diidentifikasi.
PENGARUH VARIASI WAKTU PENYIMPANAN TERHADAP STABILITAS REAGEN KERJA PADA HASIL PEMERIKSAAN KADAR KREATININ Silvi Marshela; Suryanata Kesuma; Suparno Putera Makkadafi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 11 No. 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3809

Abstract

Kidney is one of the organs of the body whose role is very important to maintain the stability of volume, osmolarity of extracellular fluid, and electrolyte composition. One of the parameters to determine kidney function that is usually done is creatinine examination. Preparation of working reagents is very important for accurate results in an examination. The purpose of this study was to determine the effect of storage on the stability of working reagents on creatinine examination. This type of research is a pseudo-experiment, to determine the difference between working reagents that are directly used with working reagents that are stored for several days. The research variables are the length of storage of work reagents, creatinine levels and storage temperature. The sample used in this study was normal creatinine control. The results were analyzed using Microsoft Excel and using the Kruskal Wallis Non Parametric test. The results of the study showed that the average reagent immediately and 5 days was 0.8 mg/dl and at 8 days 1.2 mg/dl, the accuracy obtained was -3.2% and 0.4%, the precision value obtained was 35.1% and 31.0%, in statistical tests significant results <0.05, meaning that there are differences in the results of examinations using working reagents that are used immediately and those stored for 5 and 8 days.
PENGARUH VARIASI WAKTU PENDINGINAN CARBOL FUCHSIN TERHADAP HASIL PEMERIKSAAN BASIL TAHAN ASAM Amelia Maulida; Tiara Dini Harlita; Suparno Putera Makkadafi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 11 No. 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3906

Abstract

Microscopic examination with Ziehl Neelsen staining is one way to confirm the diagnosis of tuberculosis. Ziehl Neelsen staining has 3 reagents, namely carbol fuchsin 1%, alcohol acid 3%, and methylene blue 0.1%. Based on Standard Operating Procedures, the carbol fuchsin cooling process is carried out for 5-7 minutes. The aim of this research was to determine the effect of varying carbol fuchsin cooling times of 6 minutes, 8 minutes, 10 minutes and 12 minutes on the results of BTA microscopic examination. This type of research is an experiment with a completely randomized design (CRD). The samples used were BTA (+3) sputum specimens from RSUD. Abdoel Wahab Sjahranie 3 pots. The quality assessment of BTA preparations was carried out by the East Kalimantan Provincial Health Laboratory. BTA preparations were given 4 treatments varying in carbol fuchsin cooling time, namely 6 minutes, 8 minutes, 10 minutes and 12 minutes, then repeated 6 times and analyzed using the Chi-Squared test. The results of this study were that all BTA preparations that were treated with variations in carbol fuchsin cooling time were of good quality based on 6 criteria for good preparations with an average scoring of 100% in the 6 minute treatment, 98.3% in the 8 minute treatment, 96.6% in the 8 minute treatment. 10 minutes and 95% in the 12 minute treatment. Based on the results of the Chi-Squared test analysis of cleanliness criteria, a p value of 0.000 was obtained, indicating that there was no significant influence between variations in carbol fuchsin cooling time on the results of BTA microscopic examination. Thus, during the cooling stage, carbol fuchsin can be used for a duration of 5-7 minutes to produce a good quality preparation.
GAMBARAN JAMUR Candida albicans PADA SALIVA PENDERITA DM TIPE 2 DI PUSKESMAS HARAPAN BARU SAMARINDA Puspitasari, Deanita Rabiatul Zhadiah; Makkadafi, Suparno Putera; Azahra, Sresta
Jurnal Ilmu Kedokteran dan Kesehatan Vol 10, No 11 (2023): Volume 10 Nomor 11
Publisher : Prodi Kedokteran Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jikk.v10i11.11821

Abstract

Abstrak: Gambaran Jamur Candida albicans Pada Saliva Penderita Diabetes Melitus Tipe2 Di Puskesmas Harapan Baru Samarinda. Diabetes melitus (DM) adalah penyakit kronis yang dapat diderita seumur hidup yang disebabkan oleh gangguan metabolisme. Penderita DM dengan hiperglikemia dapat mempengaruhi akumulasi saliva yang berhubungan dengan asam basa mulut (pH). Flora normal saliva dalam rongga mulut terdiri dari bakteri seperti Streptococcus sp dan jamur seperti Candida albicans. Tujuan penelitian untuk mengetahui adanya jamur C.albicans pada saliva penderita DM tipe 2 di Puskesmas Harapan Baru. Jenis penelitian ini deskriptif dengan desain cross sectional. Jumlah sampel sebanyak 33 sampel saliva penderita DM tipe 2 di Puskesmas Harapan Baru Samarinda dengan teknik purposive sampling. Sampel diidentifikasi secara makroskopis dan mikroskopis. Data akan dianalisis secara univariat. Karakteristik responden berdasarkan usia penderita DM persentase tertinggi positif jamur C.albicans pada rentang usia 45-59 tahun sebanyak 12  (52%),  pada rentang usia 60-70 tahun 5 (50%); jenis kelamin         penderita DM persentase tertinggi positif jamur C.albicans pada perempuan yaitu 15 (58%), laki-laki 2 (29%). Hasil kadar glukosa darah sewaktu penderita DM yang terinfeksi C.abicans didapatkan berada diatas normal ≥ 200 mg/dl yaitu 33 (100%). Hasil penelitian negatif jamur C.albicans 48% dengan pertumbuhan jamur Candida non albicans 12 responden dan tidak ditemukan pertumbuhan jamur sebanyak 4 responden. Dari hasil penelitian ini didapatkan hasil positif jamur C.albicans 52%.
GAMBARAN ANGKA LEMPENG TOTAL BAKTERI PADA JAJANAN PENTOL BAKAR YANG DIJUAL DI WILAYAH KECAMATAN SUNGAI KUNJANG KOTA SAMARINDA Aryanti E., Fadillah; Makkadafi, Suparno Putera; Saputri, Maulida Julia
Jurnal Ilmu Kedokteran dan Kesehatan Vol 10, No 10 (2023): volume 10 Nomor 10
Publisher : Prodi Kedokteran Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jikk.v10i10.11981

Abstract

Abstrak: Gambaran Angka Lempeng Total Bakteri Pada Jajanan Pentol Bakar Yang Dijual Di Wilayah Kecamatan Sungai Kunjang Kota Samarinda. Pentol bakar adalah produk pangan yang bahan utamanya terbuat dari olahan daging dan bahan lainnya. Batas maksimum cemaran mikroba pada bakso daging menurut SNI 3818:2014 untuk nilai ALT adalah 1 × 105 koloni/g. Faktor terjadinya kontaminasi pada pentol bakar dapat melalui udara, debu jalan, penyiapan, pengolahan serta penyajian. Penelitian ini bertujuan untuk mengetahui jumlah koloni bakteri pada jajanan pentol bakar yang dijual di wilayah Kecamatan Sungai Kunjang Kota Samarinda. Penelitian ini menggunakan desain deskriptif observasi. Teknik pengambilan sampel yang digunakan adalah secara accidental dengan jumlah sampel dari 7 pedagang. Sampel pentol bakar diambil lalu dilakukan pemeriksaan Angka Lempeng Total dengan metode tuang menggunakan media PCA. Analisis data yang digunakan adalah analisis univariat dan hasil dalam berbentuk tabel. Berdasarkan hasil penelitian didapatkan hasil dari 7 sampel tidak memenuhi syarat sesuai SNI 3818:2014 dengan hasil tertinggi pada kode sampel S1 (2,8 x 107 koloni/g). Persentase Angka Lempeng Total bakteri pada pentol bakar yang dijual di wilayah Kecamatan Sungai Kunjang Kota Samarinda yang tidak memenuhi syarat sesuai SNI 3818;2014 yaitu 100% sesuai SNI nomor 3818 tahun 2014. Dapat disimpulkan bahwa jajanan pentol bakar yang dijual di wilayah Kecamatan Sungai Kunjang Kota Samarinda tidak memenuhi syarat sesuai SNI nomor 3818 tahun 2014.
Gambaran Kadar Sgot dan Sgpt pada Pasien Tuberkulosis Paru Aktif Stephanie, Graciela; Maulida Julia Saputri , Maulida Julia Saputri; Suparno Putera Makkadafi , Suparno Putera Makkadafi
Jurnal Sains & Kesehatan Vol 3 No 1 (2024): Jurnal Sains dan Kesehatan
Publisher : Politeknik Baubau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57151/jsika.v3i1.261

Abstract

Tuberculosis (TB) is a global problem with around half a percent of the world's population infected. The number of TB cases in Samarinda City in 2021 will reach 1,464 cases. TB treatment can damage the liver, and the SGOT and SGPT enzymes are related to liver function. SGPT is more commonly found in the liver, while SGOT is found in the liver, heart, skeletal muscles, kidneys, brain and blood cells. The purpose of this study was to determine the levels of SGOT and SGPT in patients with active pulmonary tuberculosis at the Sidomulyo Health Center in Samarinda. This type of research is a descriptive observational study with 30 samples of pulmonary TB patients who are currently undergoing anti-tuberculosis drug (OAT) treatment. Sampling was carried out using total sampling technique. The variables observed were SGOT and SGPT levels. The results of measurements using spectrophotometry showed that normal AST levels were mostly found in 100% female patients, while abnormal AST levels were mostly found in male patients (53%). The highest normal SGPT levels were found in male patients (100%), while the highest abnormal SGPT levels were found in female patients (27%). The examination results also showed that the adult age group with an age range of 20-59 years had normal SGOT levels (33%) and high normal SGPT levels (96%). It can be concluded that the results of SGOT levels based on sex are abnormal in male patients, whereas based on age the results of abnormal SGOT levels in adult patients. Furthermore, SGPT levels based on sex were abnormal in female patients while based on age SGPT levels were abnormal in adolescent patients.
Gambaran Jamur Candida Sp Pada Handuk Anak Kos Pagayang, Rektoviana Andi Randa; Suparno Putera Makkadafi; Nurul Anggrieni
Jurnal Sains & Kesehatan Vol 3 No 2 (2024): Jurnal Sains dan Kesehatan
Publisher : Politeknik Baubau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57151/jsika.v3i2.421

Abstract

Towels are a necessity for everyone to dry their body and hair. Moist towels without sunlight cause mold and bacteria growth. Candida sp is an infection of the skin that usually develops on nails and body parts. This study aims to identify and determine the percentage of Candida sp fungi on towels of boarding children in Loa Jananan Ilir District conducted in March 2024, this type of research is descriptive with a Cross Sectional design. The number of samples in this study with 44 samples taken randomly Spada towels boarding children. From the identification obtained 47% of boarding towels contained Candida sp. It is necessary to pay more attention to personal hygiene, especially for boarding school children, so that health problems, especially those caused by the Candida Sp fungus, can be minimized.
Perbedaan Penggunaan Alas Kaki Terhadap Prevalensi Jamur Penyebab Onikomikosis Pada Petani Di Kelurahan Handil Bakti Palaran Nita, Khairun; Azahra, Sresta; Makkadafi, Suparno Putera
Borneo Journal of Medical Laboratory Technology Vol. 7 No. 2 (2025): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v7i2.8238

Abstract

Onikomikosis atau dikenal sebagai infeksi jamur kuku ditemukan di seluruh dunia dengan angka kejadian cukup tinggi di Indonesia. Infeksi jamur pada kuku dapat disebabkan oleh jamur dermatofita, non dermatofita dan yeast. Petani merupakan pekerjaan yang berisiko terjadinya onikomikosis karena lingkungan kerja yang berair mendukung terjadinya infeksi jamur pada kuku. Penggunaan alas kaki yang tertutup dalam waktu lama juga akan merangsang tumbuhnya jamur. Tujuan penelitian ini untuk mengetahui perbedaan penggunaan alas kaki terhadap prevalensi tumbuhnya jamur penyebab onikomikosis pada petani. Penelitian ini menggunakan studi observasional analitik dengan pendekatan cross sectional. Sampel berjumlah 60 kuku kaki petani dengan teknik purposive sampling. Pengambilan data menggunakan lembar observasi dan pengambilan kuku kaki pada petani di Kelurahan Handil Bakti Palaran. Metode pemeriksaan dilakukan pengamatan makroskopis dengan kultur jamur pada media SDA (Sabouraud Dextrose Agar) dan pengamatan mikroskopis dengan pembuatan slide kultur. Analisa data menggunakan uji chi square. Hasil penelitian didapatkan jamur yang paling banyak menyebabkan onikomikosis dari golongan non dermatofita yaitu Aspergillus sp (60,0%) dan golongan dermatofita yaitu Trichophyton sp (10,0%). Jamur penyebab onikomikosis pada petani yang menggunakan alas kaki sebesar 36,7% dan yang tidak menggunakan alas kaki sebesar 48,3% dengan analisis statistik p-value sebesar 0,026 (<0,05), maka disimpulkan terdapat perbedaan signifikan terhadap alas kaki dengan kejadian onikomikosis. Petani diharapkan dapat meningkatkan kesadaran akan pentingnya penggunaan alas kaki dan selalu menjaga pola hidup bersih dan sehat saat bekerja.
Pembelajaran Berbasis Proyek Berbentuk Terarium Dalam Botani Tingkat Rendah: Persepsi Mahasiswa Pendidikan Biologi: Project-Based Learning in the Form of a Terrarium in Lower Botany: Perceptions of Biology Education Students Makkadafi, Suparno Putera; Turista, Dora Dayu Rahma
BIODIK Vol. 11 No. 03 (2025): September 2025
Publisher : Program Studi Pendidikan Biologi FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biodik.v11i03.45997

Abstract

Lower-level botany learning has been carried out using conventional lecture methods. Learning becomes less meaningful for students because students tend to be passive. The purpose of this study was to see how students perceive the application of the project-based learning (PjBL) model in lower-level botany courses. This research was conducted using a quantitative research model with a survey method. Data collection was carried out by giving questionnaires to students. As many as 85.7% of students stated that they felt that their understanding of lower-level botany had improved and 80% expressed interest in lower-level botany learning with PjBl. As many as 82.9% stated that they were able to design a terrarium well and 85.7% were able to state that PjBl helped them identify the morphology of cryptogamae. As many as 74.3% of students stated that they were motivated by group cooperation and 71.4% of them were able to link theory and practice. The final result was that 88.6% of students felt that this learning method improved their understanding of cryptogamae. Overall, low-level botany learning with PjBL for biology education students at Mulawarman University can improve students' understanding and interest in low-level botany courses. Key words: Project-Based Learning (PjBL), Lower-Level Botany, Terrarium, Student Perception
Co-Authors Adinda Devi Nofalia Ainy Pebriani Akhmad Akhmad Akhmad Akhmad Akhmad Akhmad Akhmad Akhmad Akhmad Akhmad Aloysius Duran Corebima Aloysius Duran Corebima Aloysius, Duran Corebima Amelia Maulida Amelia Tya Nabila Amelia Tya Nabila Angellya Kristin Sapan Anggrieni, Nurul Apri Prananda Saputra Apria Rizki Pratiwi Arifai Arifta Amelia, Regita Heddy Aryanti E., Fadillah Aulia Ayu Oktavia Aulia Rizqina Azahra, Sresta Damayanti, Sulpinah Defitri Afira Dewi Suffah Anggraeni Diah Pratiwi Dinda Davanya Dora Dayu Rahma Turista Dora Dayu Rahma Turista Dora Dayu Rahma Turista Dora Dayu Rahma Turista Ehva Pahria Ummul Khaer Elsje Theodora Maasawet Elsje Theodora Maasawet Elsje Theodora Maasawet Elsje Theodora Maasawet Elsje Theodora Maasawet Endah Puji Lestari Erniyanti Erniyanti Fatchur Rohman Fatchur Rohman Ganea Qorry Aina Herliani Herliani Herliani Herliani Herliani Herliani Jailani Jailani Jailani Jailani Jailani Jailani Jailani Jailani Jailani Kesuma, Suryanata Khairida Kanaya Utami Khairunna Shofieyah Lastri, Sulastri Margaretha Dhea Kristiani Maryani, Pira Masitah - Masitah Masitah Masitah Masitah Masitah Masitah Masitah Masitah Masitah Masitah Maulida Julia Saputri , Maulida Julia Saputri muhammad, muhammad ali Nafisahtul Huda Nelda Anasthasia Serena Nita, Khairun Novia Nabila Ramadhana Novia Nur Anita Syafitri Nurhayati Nurhayati Nurhidayah Eka Widyaningrum Nursalinda Kusumawati Nurul Anggraeni Nurul Risma Widyawati Oktavianus Tae B.N Pagayang, Rektoviana Andi Randa Panca Selfi Agustina Puji Setia Wati Puspitasari, Deanita Rabiatul Zhadiah Putriyanni Putriyanni Rahmanisa Ananda Regia Dwi Anggreni Riska Rahmawati Utami Rita Purbianti Rizqi Fakhrial Husain Robiyatul Sakdiah Chesarani Rochman, Fatcur Rosinta Rosinta Rosyad, Muhammad Naufal Ruqoyyah Nasution Salma Azzahra Salsabila Desvika Nurfadhila Saputri, Maulida Julia Selvi Novita Sari Selvia Teodora Br Ginting Shafira Salsabila Indipravita Silvi Marshela Siti Maria Mawar Dinata Sonja V. T. Lumowa Sonja V. T. Lumowa Sonja Verra Tinneke Lumowa Sonja Verra Tinneke Lumowa Sonja Verra Tinneke Lumowa Sonja Verra Tinneke Lumowa Sri Purwati Sri Purwati Sri Purwati, Sri Stephanie, Graciela Suci Rahmawati Suhartini Suhartini Tiara Dini Harlita Tiara Dini Harlita Turista, Dora Dayu Rahma Vandalita Maria Magdalena Rambitan Vandalita Maria Magdalena Rambitan Vandalita Maria Magdalena Rambitan Vandalita Maria Magdalena Rambitan Vandalita Maria Magdalena Rambitan Vandalita Maria Magdalena Rambitan Zenia Lutfi Kurniawati