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All Journal Majalah Kedokteran Sriwijaya Bioscientia Medicina : Journal of Biomedicine and Translational Research Sriwijaya Journal of Surgery Bioscientia Medicina : Journal of Biomedicine and Translational Research
Mgs Irsan Saleh
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Identifikasi Polimorfisme Insersi/Delesi Gen Angiotensin Converting Enzym Intron 16 Pada Pasien Preeklampsia di RS. Dr. Muhammad Hoesin Palembang Muthmainnah Arifin; Mgs Irsan Saleh; Subandrate Subandrate
Majalah Kedokteran Sriwijaya Vol 46, No 3 (2014): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/mks.v46i3.2709

Abstract

Preeklampsia adalah penyakit kehamilan dengan gejala hipertensi dan proteinuria yang timbul pada usia kehamilan diatas 20 minggu. Preeklampsia terkait dengan polimorfisme insersi/delesi (I/D) gen angiotensin converting enzym (ACE) intron 16. Polimorfisme ini dapat meningkatkan aktivitas ACE yang mengakibatkan gagalnya remodelling arteri spiralis sehingga terjadi preeklampsia. Namun, penelitian ini belum pernah dilakukan sebelumnya pada pasien preeklampsia di RSMH Palembang. Oleh karena itu, penelitian ini bertujuan untuk mengidentifikasi polimorfisme insersi/delesi gen ACE intron 16 pada penderita preeklampsia di RSMH Palembang.Penelitian ini merupakan penelitian deskriptif observasional dengan sampel sebanyak 32 orang pasien preeklampsia. Identifikasi polimorfisme insersi/delesi (I/D) gen ACE intron 16 dilakukan dengan teknik PCR amplifikasi yang ditentukan dengan menggunakan primer spesifik. Primer tersebut menggunakan teknik mutagenesis situs terarah yang mengintroduksi mutasi titik ke suatu situs tertentu.Genotipe mutan homozigot (DD) sebanyak 0%, genotipe mutan heterozigot (ID) sebanyak 40,6%, dan genotipe wild type (II) sebanyak 59,4%. Jumlah alel I dan D gen ACE yang ditemukan masing-masing sebanyak 79,7% dan 20,3% pada pasien preeklampsia di RSMH Palembang.Genotipe wild type lebih banyak ditemukan pada pasien preeklampsia di RSMH Palembang (59,4%).
CORRELATION BETWEEN ETHAMBUTOL THERAPY DURATION AND ZINC SERUM LEVEL WITH COLOUR VISION DEFICIENCY IN TUBERCULOSIS PATIENTS Devi Azri Wahyuni; Mira Retna Tetiana; Mgs Irsan Saleh; Ahmad Rasyid
Majalah Kedokteran Sriwijaya Vol 52, No 2 (2020): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/mks.v52i2.11975

Abstract

Tuberculosis is one of the most common disease in Indonesia. Ethambutol is one of the main regiment therapy for patient with tuberculosis. Duration of ethambutol therapy depends on what category of tuberculosis patient. Ethambutol intake can cause optic neuropathy with colour vision deficiency as an early symptom. Ethambutol therapy can also cause decrease of zinc serum level and it is cause by zinc chelate that can be found in ethambutol. The purpose of this study was to determine the correlation between duration of ethambutol therapy and zinc serum level with colour vision deficiency which examined with Farnsworth Munsell 100 Hue in tuberculosis patients. This is an observational explorative study. Colour vision is xamined with Farnsworth Munsell 100 Hue and zin serum level is detected by blood sample. Data was analyzed by correlation study. Correlation statistic between ethambutol therapy duration with colur vision using Spearman coreelation study found that r = 0,5 and p = 0,00, whereas in correlation between zinc serum level with colour vision found that r = 1,68 and p = 0,32. There is significant correlation and positive direction between ethambutol therapy duration with colour vision deficiency. Even though there is no significant correlation between zinc serum level with colour vision deficiency, there is a strong correlation.
Antifungi Effect of Keghenyat Leaves Fraction (Acmella uliginosa) In Vitro Study Agustina Septi Hijir; Salni Salni; Joko Marwoto; Triwani Triwani; Sri Nita; Mgs Irsan Saleh; Ziske Maritska; Nita Parisa; Evi Lusiana; Nia Savitri Tamzil; Ayeshah Augusta Rosdah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 1 No. 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v1i1.5

Abstract

Introduction Candida is a yeast fungus classes most commonly found in the oral cavity, gastrointestinal tract, reproductive tract and skin, especially the species Candida albicans. Alternative medicines such as herbs are easily available and affordable by the community, such as keghenyat leaves (Acmella uliginosa). Aim of StudyTo determine the activity of Keghenyat leaves fraction (Acmella uliginosa) compared to Nystatin against Candida albicans in vitro. MethodsIn vitro study was held in Laboratory of Genetics and Biotechnology Department of Biology, Faculty of Science and Medical Faculty, Sriwijaya University in December 2015 and January 2016. Research stages: extraction, fractionation, sensitivity test using Nystatin and Candida albicans, fractions antifungal activity test, the determination of minimum inhibitory concentration (MIC), bioautografi test and determination of compound classes. One final stages of testing the active fraction Keghenyat leaves (Acmella uliginosa) with Nystatin. Data were analyzed using ANOVA and Post Hoc Duncan test and linear regression using SPSS 20. ResultsHexane faction of Keghenyat leaves (Acmella uliginosa) is active against the fungus Candida albicans, has a Minimum Inhibitory Concentration (MIC) of 0.625% (6250μl) against the fungus Candida albicans antifungal contain active compounds that terpenoids and flavonoids, amounting to 84.68 mg / ml equivalent to 1 mg / ml Nystatin against the fungus Candida albicans. ConclusionThere is a significance different MIC between hexane fraction of Keghenyat leaves (Acmella uliginosa) and Nystatin, Nystatin have more high activity.
Telmisartan Prevents Myocardial Fibrosis via Decreasing Fraction of Colagen Type 1 Volume in Myocardial Tissue in Wistar Rats-Induced High Salt Intake Radiyati Umi Partan; Rachmat Hidayat; Mgs Irsan Saleh; Nita Parisa; Evi Lusiana; Nia Savitri Tamzil; Ayeshah Augusta Rosdah; Muhammad Reagen
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 1 No. 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v1i1.6

Abstract

Myocardial fibrosis is a pathological condition that responsible for initiation of heart failure. Neurohormonal endogen, angiotensin II, has a potential role to activate endothelin I, TGF-β1, myocardial fibroblast, extracelullar matrix deposition, structural changes and decreasing of cardiac function. Fibrotic process is also influenced by PPAR γ. Telmisartan has a potential effect to inactivate angiotensinergic system and to activate PPAR γ. It is expected that telmisartan has optimal effect to protect myocardial fibrosis. To know the role of variation dose of telmisartan to decrease collagen type 1 fraction volume in cardiac tissue of Wistar rats. Ten-week-old male Wistar Rat (n = 30) were randomized into five groups, and each group consisted of 6 rats. Group 1 : negative control. Group 2 : rats were induced by intake Nacl 8% doses 2% body weight for eight weeks. Group 3 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 3 mg/kgBB for eight weeks. Group 4 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 6 mg/kgBB for eight weeks. Group 5 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 12 mg/kgBB for eight weeks. Collagen volume fraction was assessed by immunohistochemistry and ImageJ program. ANOVA test followed pos hoc test was used to analyzed each variable. Collagen volume fraction significantly decreased in group 3, 4 and 5 compared in group 2. Telmisartan decreases collagen type 1 volume fraction of myocardial tissue .
The Development of Prototype Polyclonal Antibody of Receptor Advanced Glycation of Endproducts (RAGE) Mgs Irsan Saleh; Nita Parisa; Ziske Maritska
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.16

Abstract

Abstract Background Receptor for advanced glycation endproducts (RAGE) is a transmembrane protein that belongs to the immunoglobulin superfamily. As its name implicates, it can bind to advanced glycation endproducts, the resulting product of non-enzymatic glycosylation, and it also has the ability to interact with multiple ligands having common motifs as a so-called multi-ligand receptor. The ligands include high-mobility group protein (B)1 (HMGB1), S-100 calcium-binding protein, amyloid-β-protein, Mac-1, and phosphatidylserine. Interaction between RAGE and its ligands activates various cellular processes, including inflammation, proliferation, apoptosis, autophagy, and migration. Methods The proccess of isolation protein of RAGE was initiated with extraction protein and purification of RAGE protein. After that, the immunization of Rats was be done to produce Anti-RAGE. The confirmation of Anti RAGE was be done by SDS PAGE and Immunobloting. Results The production of Anti RAGE was enough pure compared by Anti RAGE commercial. Anti RAGE was protein that have molecular weight around 35 kD. Conclusion The methods that used in this study effective to develop production of anti-RAGE. Keywords : Anti RAGE – Isolation – Production Antibody
The Efficacy of Combination Extract Andrographis peniculata and Syzygium polyanthum on Glucose Uptake in Skletal Muscle in Diabetic Rats Fatmawati Karim; Ester Gonzales Panserga; Mgs Irsan Saleh
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 4 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i4.63

Abstract

Insulin resistance is impaired insulin signaling cascade in target cells to respond normal or elevated circulating insulin to the final cellular effect, such as translocation of vesicles containing GLUT4 glucose transporters, which is the major mediator of glucose removal from the circulation and a key regulator of whole-body glucose homeostasis. Among the several plants, Andrographis peniculata (sambiloto) and Syzygium polyanthum (Daun salam). In the present study, a combination was made, Andrographis peniculata (sambiloto) and Syzygium polyanthum (Daun salam), and to determine the effects of combination on tretament insulin resistance. Male Wistar rats (weight, 200-300 g) were randomized into five groups (6 rats/group). Group 1: negative group. Group 2: positive group (metformin 63 mg/kgBW ). Group 3,4 and 5: treatment with extract combination, each group 250 mg/kgBW, 500 mg/kgBW and 1000 mg/kgBW. Rats were induced by high fat diet-glucocorticoid for insulin resistance. Insulin and GLUT-4 were assayed by ELISA. Treatment with extract combination (250, 500 and 1000 mg/kgBW) and metformin for 2 weeks showed a sigificant decrease in fasting plasma insulin compare with the negative control rats with a reduction of 11,2%, 33,6%, 20% and 19,4%, respectively. Two weeks treatment either extract combination or metformin in diabetic rats, significantly increased GLUT 4 level (p<0,05) with a percentage increase of 6,68%, 15,21%, 12,76% and 1,77%.
Antifungi Effect of Keghenyat Leaves Fraction (Acmella uliginosa) In Vitro Study Agustina Septi Hijir; Salni Salni; Joko Marwoto; Triwani Triwani; Sri Nita; Mgs Irsan Saleh; Ziske Maritska; Nita Parisa; Evi Lusiana; Nia Savitri Tamzil; Ayeshah Augusta Rosdah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 1 No. 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v1i1.5

Abstract

Introduction Candida is a yeast fungus classes most commonly found in the oral cavity, gastrointestinal tract, reproductive tract and skin, especially the species Candida albicans. Alternative medicines such as herbs are easily available and affordable by the community, such as keghenyat leaves (Acmella uliginosa). Aim of StudyTo determine the activity of Keghenyat leaves fraction (Acmella uliginosa) compared to Nystatin against Candida albicans in vitro. MethodsIn vitro study was held in Laboratory of Genetics and Biotechnology Department of Biology, Faculty of Science and Medical Faculty, Sriwijaya University in December 2015 and January 2016. Research stages: extraction, fractionation, sensitivity test using Nystatin and Candida albicans, fractions antifungal activity test, the determination of minimum inhibitory concentration (MIC), bioautografi test and determination of compound classes. One final stages of testing the active fraction Keghenyat leaves (Acmella uliginosa) with Nystatin. Data were analyzed using ANOVA and Post Hoc Duncan test and linear regression using SPSS 20. ResultsHexane faction of Keghenyat leaves (Acmella uliginosa) is active against the fungus Candida albicans, has a Minimum Inhibitory Concentration (MIC) of 0.625% (6250μl) against the fungus Candida albicans antifungal contain active compounds that terpenoids and flavonoids, amounting to 84.68 mg / ml equivalent to 1 mg / ml Nystatin against the fungus Candida albicans. ConclusionThere is a significance different MIC between hexane fraction of Keghenyat leaves (Acmella uliginosa) and Nystatin, Nystatin have more high activity.
Telmisartan Prevents Myocardial Fibrosis via Decreasing Fraction of Colagen Type 1 Volume in Myocardial Tissue in Wistar Rats-Induced High Salt Intake Radiyati Umi Partan; Rachmat Hidayat; Mgs Irsan Saleh; Nita Parisa; Evi Lusiana; Nia Savitri Tamzil; Ayeshah Augusta Rosdah; Muhammad Reagen
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 1 No. 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v1i1.6

Abstract

Myocardial fibrosis is a pathological condition that responsible for initiation of heart failure. Neurohormonal endogen, angiotensin II, has a potential role to activate endothelin I, TGF-β1, myocardial fibroblast, extracelullar matrix deposition, structural changes and decreasing of cardiac function. Fibrotic process is also influenced by PPAR γ. Telmisartan has a potential effect to inactivate angiotensinergic system and to activate PPAR γ. It is expected that telmisartan has optimal effect to protect myocardial fibrosis. To know the role of variation dose of telmisartan to decrease collagen type 1 fraction volume in cardiac tissue of Wistar rats. Ten-week-old male Wistar Rat (n = 30) were randomized into five groups, and each group consisted of 6 rats. Group 1 : negative control. Group 2 : rats were induced by intake Nacl 8% doses 2% body weight for eight weeks. Group 3 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 3 mg/kgBB for eight weeks. Group 4 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 6 mg/kgBB for eight weeks. Group 5 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 12 mg/kgBB for eight weeks. Collagen volume fraction was assessed by immunohistochemistry and ImageJ program. ANOVA test followed pos hoc test was used to analyzed each variable. Collagen volume fraction significantly decreased in group 3, 4 and 5 compared in group 2. Telmisartan decreases collagen type 1 volume fraction of myocardial tissue .
The Development of Prototype Polyclonal Antibody of Receptor Advanced Glycation of Endproducts (RAGE) Mgs Irsan Saleh; Nita Parisa; Ziske Maritska
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.16

Abstract

Abstract Background Receptor for advanced glycation endproducts (RAGE) is a transmembrane protein that belongs to the immunoglobulin superfamily. As its name implicates, it can bind to advanced glycation endproducts, the resulting product of non-enzymatic glycosylation, and it also has the ability to interact with multiple ligands having common motifs as a so-called multi-ligand receptor. The ligands include high-mobility group protein (B)1 (HMGB1), S-100 calcium-binding protein, amyloid-β-protein, Mac-1, and phosphatidylserine. Interaction between RAGE and its ligands activates various cellular processes, including inflammation, proliferation, apoptosis, autophagy, and migration. Methods The proccess of isolation protein of RAGE was initiated with extraction protein and purification of RAGE protein. After that, the immunization of Rats was be done to produce Anti-RAGE. The confirmation of Anti RAGE was be done by SDS PAGE and Immunobloting. Results The production of Anti RAGE was enough pure compared by Anti RAGE commercial. Anti RAGE was protein that have molecular weight around 35 kD. Conclusion The methods that used in this study effective to develop production of anti-RAGE. Keywords : Anti RAGE – Isolation – Production Antibody
The Efficacy of Combination Extract Andrographis peniculata and Syzygium polyanthum on Glucose Uptake in Skletal Muscle in Diabetic Rats Fatmawati Karim; Ester Gonzales Panserga; Mgs Irsan Saleh
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 4 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i4.63

Abstract

Insulin resistance is impaired insulin signaling cascade in target cells to respond normal or elevated circulating insulin to the final cellular effect, such as translocation of vesicles containing GLUT4 glucose transporters, which is the major mediator of glucose removal from the circulation and a key regulator of whole-body glucose homeostasis. Among the several plants, Andrographis peniculata (sambiloto) and Syzygium polyanthum (Daun salam). In the present study, a combination was made, Andrographis peniculata (sambiloto) and Syzygium polyanthum (Daun salam), and to determine the effects of combination on tretament insulin resistance. Male Wistar rats (weight, 200-300 g) were randomized into five groups (6 rats/group). Group 1: negative group. Group 2: positive group (metformin 63 mg/kgBW ). Group 3,4 and 5: treatment with extract combination, each group 250 mg/kgBW, 500 mg/kgBW and 1000 mg/kgBW. Rats were induced by high fat diet-glucocorticoid for insulin resistance. Insulin and GLUT-4 were assayed by ELISA. Treatment with extract combination (250, 500 and 1000 mg/kgBW) and metformin for 2 weeks showed a sigificant decrease in fasting plasma insulin compare with the negative control rats with a reduction of 11,2%, 33,6%, 20% and 19,4%, respectively. Two weeks treatment either extract combination or metformin in diabetic rats, significantly increased GLUT 4 level (p<0,05) with a percentage increase of 6,68%, 15,21%, 12,76% and 1,77%.
Co-Authors Afifurrahman Agustina Septi Hijir Ahmad Rasyid Akbar, Hersusiad Ayeshah A Rosdah Devi Azri Wahyuni Ester Gonzales Panserga Fatmawati Jayawarsa, A.A. Ketut Joko Marwoto Mira Retna Tetiana Muhammad Reagen Mukmin, Sugianto Muthmainnah Arifin Nita Parisa Novadian Nur Riviati, Nur RACHMAT HIDAYAT Radiyati Umi Partan Salni Salni Sri Nita Subandrate Triwani Triwani Wiria Aryanta Yundari, Yundari Ziske Maritska