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Journal : IJOBI

THE EFFECTIVITY OF EM4 MADE FROM BACTERIA Bacillus sp. FOR COMPOSTING HOUSEHOLD WASTE AND ON THE GROWTH OF CAYENNE PEPPER PLANT (Capsicum frutescens L) Saputri, Maulin Otto; Suhandoyo, Suhandoyo
Indonesian Journal of Bioscience (IJOBI) Vol. 2 No. 1 (2024): Indonesian Journal of Bioscience (IJOBI)
Publisher : Department of Biology Education FMIPA Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v2i1.620

Abstract

This study aims to determine the influence of differences in EM4 dosage made from Bacillus sp. and cow dung against composting household waste, to find the best dose to speed up household waste composting, and to test the resulting compost on the growth of cayenne pepper plants (Capsicum frutescens L). This is a 2-stage experiment. The first stage is the effectiveness of composting using a Completely Randomized Factorial design, and the second stage is a biological test by looking at the growth of chili plant seeds using a Completely Randomized Design. The object of this research is household waste that has not undergone decomposition, incubated in the same tub with 4 different dosage levels ( 0%, 5%, 10%, and 15%), and 3 different treatments with cow dung (1:2 and 1:4). Each treatment was repeated 3 times. This treatment lasted 41 days, followed by the second stage, where the resulting compost was used for cayenne pepper. Each treatment was carried out 5 times over 15 days with 4 observations. The parameters observed were the physical results of the compost (color, odor, and structure), temperature, composting pH, plant height, number of leaves, wet weight, and dry weight of cayenne pepper plants. Data were analyzed descriptively in stages one and two using One-way ANOVA, then continued with the Duncan Multiple Range Test (DMRT). The results showed that using EM4 made from Bacillus sp. mixed with cow dung affects the quality of the composting results. The optimal concentration for making household waste fertilizer is 10%. Household waste fertilizer significantly affects the growth of cayenne pepper plants, as seen from all observation parameters, namely plant height, number of leaves, fresh weight, and dry weight.
Tolerance of Earthworms (Eudrilus eugeniae) to 2,4-D Dimethyl Amine Herbicide Suhandoyo; Aji, Gesang Putranto Dwi
Indonesian Journal of Bioscience (IJOBI) Vol. 2 No. 2 (2024): Indonesian Journal of Bioscience (IJOBI)
Publisher : Department of Biology Education FMIPA Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v2i2.1055

Abstract

The purpose of this study was to determine (1) the tolerance of earthworms (Eudrilus eugeniae) to the herbicide 2,4-D dimethyl amine, and (2) the response of increased biomass, behavior, and morphology of earthworms (Eudrilus eugeniae) when exposed to subletal doses of the herbicide 2,4-D dimethyl amine. This research is an experimental research conducted in two stages of research. The first stage to look at herbicide toxicity, includes LD50, upper threshold, and lower threshold. The second stage is a follow-up test study using a one-factor Complete Random Design (RAL) to determine the effect of subletal dose of LD50-48 hours on the increase in biomass, behavior, and morphology of earthworms (Eudrilus eugeniae). The object of the study is the earthworm (Eudrilus eugeniae) which already has a clitelum with a biomass of 25 grams for each treatment tank. Data analysis was carried out with Proit Analysis to determine the upper threshold, lower threshold, and LD50 value while the analysis of the difference in earthworm biomass from the treatment group was carried out using  the One-Way ANOVA test  at a significance level of 95%. The average treatment was further tested using the DMRT test in the SPSS version 24.0 program. The results showed that: (1) earthworm tolerance to the herbicide 2,4-D dimethyl amine, at the  upper threshold value of 9996.014 ppm, the lower threshold of 3409.972 ppm, the LD50-48 hours of 9015.858 ppm which is practically non-toxic, (2) the response of earthworms (Eudrilus eugeniae) when exposed  to subletal doses of the herbicide 2,4-D dimethyl amine herbicideIt is characterized by a reduced earthworm appetite, a shrinking body shape, and a decrease in biomass.