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Application of Biofertilizer Consortium of Strain Bacillus sp with Different Concentrations and Aplication Methods on Growth of Nutmeg Seeds (Myristica fragrans Houtt) A. Marthin Kalay; Henry Kesaulya; Abraham Talahaturuson; Herman Rehatta; Reginawanti Hindersah
Agrologia Vol 9, No 1 (2020): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v9i1.1060

Abstract

The growth of nutmeg seeds requires adequate nutrition by mechanism of bacteria activity associated with roots and leaves. The aim of study was to determine the effect of application of bacillus strain consortium biofertilizer on nutmeg seedlings. The experiment was arranged by using completely randomized design with 0% of PBH as control, and 0.15% and 0,30% of PBH concentration by spraying to leaves and by pouring in the soil around the stem, respectivelly. The results showed that the application of 0.15% and 0.30% of PBH concentration has significant effect in plant height, shoot dry weight, dry weight root and plant dry weight, but not at the number of root, root length, stem diameter, and the ratio of shoot and root dry weight of nutmeg seedlings after four month planting in the polybag. The concentration of PBH 0.30% has a greater influence on the nutmeg seed growth, and can be a solution to increasee  seed growth. 
Aplikasi Pupuk Hayati Konsorsium Dan Inokulan Padat Trichoderma harzianum Terhadap Produktivitas Tanaman Sawi Pada Lahan Terkontaminasi Rhizoctonia solani A Marthin Kalay; M Riadh Uluputty; Juniart Leklioy; Reginawanti Hindersah; Abraham Talahaturuson
Agrologia Vol 5, No 2 (2016): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v5i2.185

Abstract

Choy sum (Brassica rapa L.) cultivation main obstacle in Maluku was soil fertility and plant diseases. Biological agent and Biofertilizers will play a significant role to reduce the intensity of disease attacks, and increase crop productivity. The objective of this experiment  was to study the effects of nitrogen fixing and phosphate solubilizing microbe (Bion-UP) and Trichoderma harzianum solid inokulan (Bokelas Plus) on choy sum productivity grown on land Rhizoctonia solani-contaminated land. The experimental treatments  were liquid  biofertilizer and Bokelas Plus which has been combined into 6 treatments: Without biofertilizer and Bokelas Plus, 50 kg Bokelas Plus/ha without biofertilizer, 15 mL biofertilizer per plant without Bokales Plus, 15 mL biofertilizer per plant + 50 Kg Bokelas Plus/ha, 15 mL biofertilizer  per plant + 25 kg Bokelas Plus/ha, and 7.5 mL biofertilizer per plant + 50 kg Bokales Plus/ha. The results showed that giving of Bokelas Plus and biofertilizer with different dose levels decreased  leaf blight intensity and increasing fresh weight of choy sum. Biofertilizer application with and without Bokelas Plus decreased the intensity of leaf blight disease by 39.14% - 46, 93%, and increase the fresh weight of choy sum by 15.19% - 36.25%. 
Effect of Biological Fertilizer and Chemical Fungicide on Fusarium Wilt Disease, Growth and Yield on Onion (Allium ascaloncum L) Rafit Syarifudin; A.Marthin Kalay; Costanza Uruilal
Agrologia Vol 10, No 2 (2021): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajibt.v10i2.1426

Abstract

Onion (Allium Ascalonicum L) are one of the leading horticultural commodities that have economic value. In cultivation, Fusarium wilt disease is often damaged caused by the fungus Fusarium oxysporum. This study aimed to examine the effect of biofertilizer application on fusarium wilt disease, growth and yield of onion. The treatments tested were Bion-up biofertilizer, KIBRT, Biostimulant, Azoto-Tricho, anthracol fungicide, and without biological fertilizer as a control. Using a randomized block design with three replications. Observation variables were the incidence of fusarium wilt disease, plant height, number and weight of tubers. The results showed that the application of biofertilizers (Bion up, KIBRT, Biostimulants and Azoto-Tricho) was effective in increasing plant height, number of bulbs, and bulb weight of onion bulbs, while controlling the development of fusarium wilt disease was more effective when using Bion up, KIBRT, and Azoto-Tricho.Keywords: Biofertilizer, Antracol, Shallots, Fusarium oxysporum
Efek Aplikasi Pupuk Hayati terhadap Populasi Mikroba dan Ketersediaan Unsur Hara Makro pada Tanah Entisol A. Marthin Kalay; Alexander Sesa; Adelina Siregar; Abraham Talahaturuson
Agrologia Vol 8, No 2 (2019): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v8i2.1011

Abstract

Penggunaan mikroba sebagai pupuk hayati untuk meningkatkan produktivitas tanaman merupakan tindakan budidaya yang efisien, ramah lingkungan dan berkelanjutan. Penelitian ini bertujuan untuk pupuk hayati konsorsium yang berasal dari akar bambu (PHK-AB), akar rumput gajah (PHK-ARG) dan pupuk hayati konsorsium Bion Up (PHK-Bion Up) dengan konsentrasi berbeda terhadap populasi total jamur dan bakteri; dan ketersediaan unsur hara makro pada tanah entisol setelah tanaman sawi.  Perlakuan yang dicobakan adalah tanpa perlakuan (kontrol); aplikasi PHK-Bion UP, PHK-AB dan PHK-ARG masing-masing dengan dengan konsentrasi 1%, 1,5%, dan 2%.  Hasil penelitian ditemukan bahwa pemberian pupuk hayati konsorsium Bion  UP, pupuk hayati asal akar bambu dan pupuk hayati konsorsium asal akar rumput gajah dapat : (1) menurunkan populasi total jamur tetapi meningkatkan populasi total bakteri; (2) pH dan kandungan N-total tanah tidak mengalami perubahan; (3) kandungan P2O5 dan K2O tersedia tanah mengalami peningkatan.  Pnelitian ini menjelaskan bahwa perbedaan konsentrasi antara pupuk hayati tidak memberikan pengaruh terhadap populasi total jamur dan bakteri, pH, kandungan N-total, dan kandungan P2O5 dan K2O tersedia tanah.Kata kunci: akar bambo, akar rumput gajah, biokontrol, pupuk hayati.
Pemberian Pupuk Hayati dengan Interval Waktu Aplikasi Berbeda terhadap Perkembangan Penyakit Rebah Semai dan Pertumbuhan Sawi (Brasica Rapa) di Pesemaian Silvia Kilian; A. Marthin Kalay; Abraham Talahaturuson
Jurnal Agroekoteknologi Vol 14, No 2 (2022)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v14i2.17506

Abstract

Seedling fall disease caused by the fungus Rhizoctonia solani is one of the limiting factors in the cultivation of mustard plants. This pathogen can attack plants in the nursery as well as after the plants in the field. The aim of this study was to determine the effect of using biological fertilizers Azoto-Trico and KIBPF with different application time intervals on the development of seedling fall disease and the growth of mustard in the nursery. The study used Azoto-Tricho and KIBPF biological fertilizers with application intervals of 2 days, 4 days and 6 days. The results showed that the application of biological fertilizers Azoto-Tricho and KIBPF with different application time intervals had an effect on controlling seedling fall disease and increasing plant height and plant fresh weight. The application of Azoto-Tricho biofertilizer and KIBPF if applied at intervals of 2 days or 4 days or 6 days had the same ability to control seedling fall disease and increase plant height and fresh weight of mustard plants in nurseries.
Effects of Soaking Rice Seeds with Biofertilizers on Brown Spot Disease Drechslera oryzae and Sprout Growth. Risanti Eka Liasaputri; A. Marthin Kalay; Hanry R. D. Amanupunyo
Agrologia Vol 12, No 1 (2023): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajibt.v12i1.1804

Abstract

Rice (Oryza sativa L.) is a staple food crop for the majority of the population in Indonesia. In rice cultivation, brown spot disease is often damaged by the fungus Drechslera oryzae. This study aims to examine the effect of applying biofertilizers on brown spot disease and growth of rice sprouts. The treatments tried were Bion-up, KIBRT, Azoto-Tricho, biofertilizers and propineb fungicide as a comparison. The experimental design used a completely randomized design with four replications. The observed variables were disease intensity, shoot height, root length, and fresh weight of sprouts. The results showed that the application of biological fertilizers Bion up, KIBRT and Azoto-Tricho was effective in controlling brown spot disease and increasing growth in shoot height, root length and sprout fresh weight.
Selection and Bioassay of Azotobacter sp. Isolates to Improve Growth of Chili (Capsicum annum L.) on Entisols in Ambon REGINAWANTI HINDERSAH; PRIYANKA PRIYANKA; WILHELMINA RUMAHLEWANG; A MARTHIN KALAY
Microbiology Indonesia Vol. 10 No. 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (677.784 KB) | DOI: 10.5454/mi.10.4.2

Abstract

Leafy vegetables contributes to the inflation rate in Ambon City due to low productivity in rainy season. Some vegetables are imported from other islands while importantvegetables such as local petsai (Brassica chinensis L.) and chili (Capsicum annum L.) are cultivated in low nitrogen soil, Entisols. Lack of nitrogen could be overcome by using inorganic fertilizeras well as biofertilzer. The soil can be inoculated with rhizobacteria, such as Azotobacter, to increase  the nitrogen uptake and improve the quality of vegetables. This research was conducted to isolate and select Azotobacter from rhizosphere of vegetables and to examine the effect of Azotobacter inoculation on chili-seedling growth and nitrogen uptake by using bioassay method. Azotobacter sp. was isolated in nitrogen-free Ashby’s Media. The bioassay was held in the green house with randomized block design experiment, which examined the combination of isolates and population of Azotobacter sp. on chili. Two best isolates which was selected based on pH, nitrogen content and cell viability were s2a10 (from petsai's rhizosphere) and c2a9 (from chili’s rhizosphere). Bioassay showed that Azotobacter inoculation followed by reduced NPK fertilizer doses had no effect on transplant dry weight and nitrogen uptake. All Azotobacter 8 -1inoculation except  10 CFU mL s2a10 maintain soil nitrogen although Azotobacter population in soil was slightly reduced. This showed that Azotobacter sp. potentially reduce the use of inorganic biofertilizer.
The potential of legume cover crops and soil microbes for gold mine tailings revegetation Triyani Dewi; Sukarjo Sukarjo; C O Handayani; Reginawanti Hindersah; Edwen D Waas; A M Kalay; Hidayatuz Zu’amah
Journal of Degraded and Mining Lands Management Vol 10, No 4 (2023)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2023.104.4593

Abstract

Mercury (Hg) is commonly utilized in artisanal gold mining on Buru Island; the Hg-contaminated tailing possibly contaminates the agricultural land nearby. In general, tailings contain very low organic carbon and plant nutrients but are high in mercury and have extreme soil acidity. The objective of this study was to observe the growth of various legume cover crops (LCC) and the change of Hg in tailing inoculated with Azotobacter-Trichoderma. The field trial was conducted on Buru Island of Maluku by using a split-plot design with three replications. The main plot was LCC species, composed of Centrosema pubescens (CP), Mucuna sp. (MC), and Crotalaria sp. (CR); the subplots were microbial inoculants composed of two formulations of Azotobacter-Trochoderma inoculants. The results showed that the Mucuna sp. and consortia Azotobacter-Trichoderma (2:1) had the highest survival rate in the tailings and Hg uptake by 8.83 mg kg-1 per plant. Consortia Azotobacter-Trichoderma inoculant with the composition of 2:1 (v:v) was able to increase soil pH, total bacterial population, LCC biomass, and Hg uptake by LCC plants. The highest Hg removal effectivity was observed in the plot treated with Crotalaria sp. and in a plot with Azotobacter-Trichoderma (2:1), which was 34.0% and 33.6%, respectively.
Efek Penggunaan Metabolit Sekunder Trichoderma harzianum terhadap Penyakit Busuk Buah Phomopsis, Hama Perusak Daun Epilachna, dan Hasil Tanaman Terung A. Marthin Kalay; Jeffij Hasinu; Widia Eka Putri; Abraham Talahaturuson
Jurnal Agroekoteknologi Vol 15, No 1 (2023)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v15i1.20003

Abstract

Upaya peningkatan produktivitas terung seringkali terkendala kerusakan tanaman oleh serangan fitopatogen dan hama. Pemanfaatan metabolit sekunder agens hayati untuk pengendalian fitopatogen dan hama perusak pada tanaman, merupakan cara pengendalian yang ramah lingkungan. Penelitian ini bertujuan untuk mendapatkan konsentrasi metabolit sekunder Trichoderma harzianum yang efektif untuk mengendalikan penyakit busuk buah Phomopsis vexans dan hama perusak daun Epilachna, serta untuk meningkatkan produksi terung. Perlakuan yang dicobakan adalah metabolit sekunder T. harzianum dengan konsentrasi 0%, 10%, 20% dan 30%. Hasil penelitian menunjukkan bahwa perlakuan metabolit sekunder T. harzianum menekan penyakit busuk buah Phomopsis, tetapi tidak berpengaruh terhadap kerusakan daun Epilachna. Pengobatan dengan konsentrasi 10%, 20% dan 30% masing-masing dapat menurunkan intensitas penyakit 51.65%, 64.25% dan 69.63%. Kerusakan daun oleh Epilachna sp tergolong ringan untuk semua tingkat konsentrasi yakni berkisar 3.65-4.32%. Perlakuan metabolit sekunder T. harzianum dengan berbagai tingkat konsentrasi berpengaruh terhadap panjang buah tetapi terhadap jumlah buah, diameter buah, dan berat buah tidak berpengaruh
PERAN AGEN HAYATI AZOTOBACTER-TRICHODERMA TERHADAP PERTUMBUHAN DAN HASIL CABAI MERAH (Capsicum annuum L.) PADA PERCOBAAN POT Reginawanti Hindersah; Priyanka Asmiran; June Putinella; Wilhelmina Rumahlewang; Marthin Kalay
Agric Vol. 29 No. 2 (2017)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2017.v29.i2.p137-146

Abstract

Mikroba tanah dapat digunakan sebagai input pertanian. Rizobakteri Azotobacter pemfiksasi nitrogen menjaga ketersediaan unsur hara nitrogen dan pertumbuhan tanaman sedangkan Trichoderma mampu menurunkan kejadian penyakit tular tanah melalui aktivitas antagonistik. Percobaan pot ini bertujuan untuk mendapatkan dosis dan waktu aplikasi inokulan Azotobacter- Trichoderma yang dapat meningkatkan ketersediaan nitrogen tanah, pertumbuhan dan hasil tanaman cabai (Capsicum annuum L.). Bibit cabai ditanam di tanah dengan kesuburan rendah yang dicampur dengan pupuk kotoran sapi. Rancangan percobaan adalah rancangan petak terbagai yang menguji empat taraf dosis inokulan dan tiga waktu aplikasi. Hasil percobaan memperlihatkan bahwa tidak ada pengaruh agen biologi terhadap tinggi tanaman pada tiga dan enam minggu setelah tanam. Inokulasi 7,5 g pot-1 inokulan padat Azoto-Tricho pada saat tanam secara signifikan meningkatkan kadar NO3 - tanah jika diikuti dengan penyiraman inokulan cair Azotobacter ke tanah pada 10 hari setelah tanam. Inokulan padat Azoto-Tricho meningkatkan hasil tanaman. Hasil tertinggi, 290 g per tanaman diperoleh dari tanaman yang diberi 7,5 g Azoto-Tricho pada saat tanam diikuti aplikasi inokulan cair Azotobacter. Percobaan pot ini menjelaskan bahwa inokulan padat Azotobacter-Trichoderma berpotensi untuk digunakan sebagai agen hayati pada produksi cabai.