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NITROGEN FIXING BACTERIA AZOTOBACTER AS BIOFERTILIZER AND BIOCONTROL IN LONG BEAN Reginawanti Hindersah; Marthin Kalay; Abraham Talahaturuson; Yansen Lakburlawal
Agric Vol. 30 No. 1 (2018)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2018.v30.i1.p25-32

Abstract

Azotobacter is Plant Growth Promoting Rhizobacteria through the mechanism of nitrogen fixation and phytohormon production but this rhizobacteria has a role to control plant diseases. The objective of experiment was to evaluate the activity of Azotobacter as biofertilizers as well as biocontrol on long bean cultivation in damping off endemic land in Ambon city, Maluku Province. The field experiment was arranged in completely randomized block design. Inoculation of long bean by Azotobacter has been done by seed inoculation, soil inoculation before planting, and plant inoculation. Plants treated with Azotobacter received fertilizer NPK of ¾ or ½ dosage recommendation while control plants were received 100% NPK. Research showed that no differences between yield of long bean inoculated with Azotobacter sp +reduced doses of NPK with that of control plants. Any application method of Azotobacter inoculation lowered damping off diseases incidence significantly until 10 days after planting, but no effect of inoculation on late blight at 21 days after planting. This study confirmed that Azotobacter has dual activity to reduce the dose of NPK fertilizer and control damping off.
PEMANFAATAN PUPUK HAYATI DAN BAHAN ORGANIK TERHADAP PERTUMBUHAN DAN HASIL TANAMAN JAGUNG MANIS (Zea mays saccharata) A. Marthin Kalay; Reginawanti Hindersah; Irene A. Ngabalin; Marina Jamlean
Agric Vol. 32 No. 2 (2020)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2020.v32.i2.p129-138

Abstract

Growth and production of corn plants require land with sufficient nutrients. Utilization of biological fertilizers and organic materials carried out with the aim of increasing the yield of corn. The treatments that were tried were the provision of compost, livestock manure, compost and livestock manure, each of which was added by spraying a consortium of biological fertilizer. The experiment was designed using a randomized block design with five replications. The results showed that the application of organic-based fertilizers affected plant height from 17 - 49 days after planting, weight of fruit with kelobot, weight of cob and ear length. Provision of compost or livestock manure and added with a biological fertilizer consortium by spray gives a higher effect compared to the provision of compost or livestock manure which is not added to the consortium of biological fertilizer. To increase the productivity of maize plants it is recommended to use a consortium of biological fertilizers applied by spraying at intervals of seven days until the plants are 49 days after planting.
Biopriming Dengan Agens Hayati Pada Benih Padi Terkontaminasi Fitopatogen Drechslera oryzae Kalay, A. Marthin; Patty, Jogeneis; Talahaturuson, Abraham; Marasabessy, Deasy
Jurnal Agroekoteknologi Vol 15, No 2 (2023)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v15i2.22453

Abstract

Fungi Dreschslera oryzae or Helminthosporium oryzae often found in rice seeds and causes brown spot disease (brown spot disease). Symptoms of attack can be seen in nurseries and on mature plants. The aim of the research is to determine the effect of a consortium of biological control agents and secondary metabolites Trichoderma harzianum which is used as biopriming on rice seeds contaminated with D. oryzae. The treatment tried was without biological control agents as a control, consortium T. harzianum and A. chrococcum, consortia of three isolates Bacillus sp, consortium A. chrococcum, A. vinelandi, Azospirillum sp, Pseudomaonas cepacia, Penicillium sp, Acinetobacter sp, and secondary metabolites T. harzianum, designed using a Completely Randomized Design with five replications. The experiment used two methods, namely germinating rice seeds on gauze media and on soil media. The research results found that biopriming with the biological control agents and secondary metabolites T. harzianum on rice seeds, it has the effect of increasing shoot height, shoot fresh weight, seedling fresh weight, root length, and reducing disease intensity. In general, the use of the biological control agents and secondary metabolites T. harzianum has the same effect. The presence of chitinase enzymes and siderophore compounds in biological control agents and secondary metabolites T. harzianum has an effect on reducing disease intensity, while the hormones auxin and gibberellin have an effect on increasing shoot height, shoot fresh weight, seedling fresh weight and root length. 
Peran Eksopolisakarida Azotobacter dan Bahan Organik untuk Meningkatkan Nodulasi dan Biomassa Kedelai pada Dua Ordo Tanah Hindersah, Reginawanti; Rostini, Neni; Kalay, Agustinus Marthin; Harsono, dan Arief
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 2 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.77 KB) | DOI: 10.24831/jai.v47i2.23328

Abstract

Exopolysaccharide (EPS) produced by nitrogen-fixing bacteria Azotobacter protect nitrogenase from oxygen. In legume,EPS plays a role in the immobilization of rhizobia to the roots. The objective of this experiment was to study the effect of EPSAzotobacter and organic matter on increasing number of nodules and biomass of soybeans grown in Inceptisols and Ultisols;as well as nitrogen-fixing bacteria population in soybean rhizosphere. The experiment was set up in a completely randomizedblock design with five replications to test combined treatments of two doses of crude EPS and organic matter. Nodule number,shoot dry weight and nitrogen uptake, as well as Azotobacter and Rhizobium population in soybean grown in Inceptisolsfollowing crude EPS and compost application, were higher than those grown in Ultisols. The application of EPS and compostIn Ultisols did not affect the number of nodule and other traits, but in Inceptisols, adding 6.25 g of compost and 20 mL of EPSto each plant increased the number of nodules and shoot weight at 42 days after planting. However, the highest N uptake wasdemonstrated by soybean received 10 mL and 20 mL EPS along with 12.5 g compost.