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Pertumbuhan Jaringan Stipe dari Jamur Sagu (Volvariella sp) Endemik Papua dalam Kultur in vitro Abbas, Barahima; Listyorini, Florentina Heningtyas; Martanto, Eko Agus; Renwarin, Yanuarius
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Sago mushrooms are edible fungus which it grew naturally on decaying sago pith. The objectives of the research were defined an appropriate formulation medium for growth and development of stipe tissue of sago mushroom in order to result spawn stages. The parent of the tissues used as explants were selected from the best performance of sago mushroom which grew naturally. The research divided into three experiments: 1) Initiation of growth hyphae from stipe tissue; 2) hyphae proliferation; and 3) spawn formation. Murashige and Skoog (MS) and Knudson-C (KN) medium enriched 15 and 30% coconut water are the best medium for growth initiation and proliferation of hyphae. Both of these medium induced the explant grew rapidly and resulted vigorous hyphae. No different effect among concentration treatment of coconut water was observed for initiation and proliferation hyphae. The fact that all of the formulation medium used for spawn formation were able to induce spawn development become spawn stage. Even so, the best mediums for spawn formation were medium formulation 9 and 10.

Karakteristik Jamur Sagu (Volvariella sp.) Endemik Papua Abbas, Barahima; Listyorini, Florentina Heningtyas; Martanto, Eko Agus
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Sago mushrooms (Volvariella sp.) were known as edible mushroom which grow sporadically on decay of sagopith waste in Papua. Local people in Papua always hunting and harvesting sago mushrooms which are growingwild on decayed sago pith waste. People were enjoyed consumption of sago mushrooms. The aims of this researchwere to study nutrient contents of sago mushrooms as well as to distinguish the difference of morphologicalcharacters between sago mushroom and paddy straw mushroom which popular in Indonesia. Sago mushroomswere collected from Yapen regency, Papua Province and measured for their nutrient contents and morphologicalcharacteristics. The results of this research showed that morphological characteristic of sago mushroom differfrom paddy straw mushroom and other edible mushroom. Nutrient contents of Sago mushroom per 100 gramsfresh weigh were follows: protein 4.00 g, carbohydrate 2.99 g, lipid 0.19 g, calcium 11.53 mg, phosphorous 0.31 g,and potassium 165.05 mg. Nutrient contents of sago mushroom were very different from that of paddy strawmushroom and others mushrooms of the genus Volvariella. Based on morphological characteristic and nutrientcontents of Sago mushrooms, we suggest that sago mushrooms is a new species.

Hirarki dan Diferensiasi Genetik Tanaman Sagu di Indonesia Berdasarkan Penanda RAPD Barahima Abbas; Muhammad Hasyim Bintoro; , Sudarsono; Memen Surahman; Hiroshi Ehara
Zuriat Vol 20, No 1 (2009)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v20i1.6643

Penelitian ini bertujuan untuk mengungkapkan hirarki dan diferensiasi genetik tanaman sagu berdasarkan penanda molekuer RAPD. Keragaman genetik tanaman diungkapkan dengan menggunakan 10 jenis primer RAPD yang diamplifikasi dengan menggunakan mesin PCR. Hasil penelitian menunjukkan bahwa ditemukan sebanyak 86 lokus dari 10 primer RAPD yang digunakan pada 100 individu sampel tanaman sagu. Hirarki genetik tanaman sagu menunjukkan bahwa pada level antar individu dan populasi berbeda sangat nyata, sedang pada level pulau berbeda nyata. Diferensiasi genetik sampel tanaman sagu menunjukkan bahwa populasi tanaman sagu mengalami diferensiasi berdasarkan nilai probabilitas Fst dan exact test (chisquare). Populasi dari Pontianak berbeda nyata untuk semua populasi begitu pula populasi dari Selat Panjang berbeda nyata dengan semua populasi kecuali populasi dari Bogor. Hasil kalkulasi diferensiasi genetik menunjukkan bahwa sampel telah mengalami diferensiasi pada level individu, populasi. Berdasarkan observasi, tiap-tiap populasi sagu di tiap-tiap pulau perlu dipertimbangkan sebagai sumber plasma nutfah untuk program kegiatan koleksi plasma nutfah sagu.

Kloning dan karakterisasi daerah promoter gen penyandi ADP glucose pyrophosphorylase dari Metroxylon sagu rendemen pati-tinggi dan -rendah [Cloning and characterization of promoter region of ADP glucose pyrophosphorylase-encoding gene from Metroxylon sagu with high- and low-starch content] Asmini BUDIANI; Riza Arief PUTRANTO; Hayati MINARSIH; Imron RIYADI; . SUMARYONO; Barahima ABBAS
E-Journal Menara Perkebunan Vol 84, No 1: Oktober 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v84i1.200

ADP-glucose pyrophosphorylase (AGPase) is one of the key enzymes in the starch biosynthesis. In many plants, the activity of this enzyme was reported to affect the yield and composition of the produced starch. This research is a part of an effort to develop molecular markers for early selection of high starch-yielding of sago palm. The purpose of the research was to isolate promoters of AGP gene and to analyze the differences in their DNA sequences between sago palm with high starch content (MsHS) and low starch content (MsLS). DNA was isolated and purified from the leaves of the two sago palm. The promoter region of AGP was amplified by Genome Walking technique. The specific primers were designed by Primer3 program based on the information of DNA sequence of AGP genes of sago palm from previous studies. Selected DNA fragments resulted from Genome Walking were isolated from the gel, cloned into E. coli, and analyzed its DNA sequence. DNA sequence analysis showed that one DNA fragment from MsHS (± 1500 bp) and one DNA fragment from MsLS (> 2000 bp) were confirmed as a 5’ upstream of the AGP gene. Further in silico analysis using MEME program identified various DNA motifs of cis-acting elements, which confirmed that those DNA fragment were promoter region of the gene. Preliminary analysis showed the differences in DNA sequences and motives of cis-acting elements in the promoter region of the two samples which might influence or indirectly associated with the character of the starch yield in sago palm.

Ekspresi dan kloning gen penyandi ADP-Glucose Phyrophosphorylase dari tanaman sagu (Metroxylon sagu Rottb.) Expression and cloning of gene encoding ADP-Glucose Phyrophosphorylase from sago palm (Metroxylon sagu Rottb.) Asmini BUDIANI1; Riza Arief PUTRANTO; Hayati MINARSIH; Imron RIYADI; . SUMARYONO; Barahima ABBAS
E-Journal Menara Perkebunan Vol 83, No 2: Desember 2015
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractSago palm (Metroxylon sagu Rottb.) is a potential food and energy resources becouse it is the highest starch producing plant. Breeding of sago palm should be directed to produce elite genotype with superior characters such as high starch content, wider pith diameter, without spine and high starch quality. However, research on sago palm in Indonesia so far is limited espescially in the field of cultivation and breeding, and attempt to produce such elite would take long time. Availability of molecular marker for starch content would be beneficial to shorten the length period of breeding. ADP-Glucose Phyrophosphorylase is one of the important enzymes in starch biosynthesis. Therefore its gene is an interesting subject in order to develope molecular marker of high starch content. This research was aimed to study the expression of gene encoding AGP in the sago palm with high starch content versus low starch content, and to clone the full cds of the gene. RNA was isolated from leaf and pith of both palms. Exspression analysis and amplify-cation of full cds were conducted by Reverse Transcryptase-Polymerase Chain Reaction (RT-PCR) using specific primers. The results showed that sago palm with higher starch content expressed AGP higher than that of sago palm with lower starch content. Expression of AGP in the full developing leaf was higher than in the young leaf, and there was no expression detected in the pith. The full cds of AGP was successfully amplified and cloned. Even though the DNA sequence showed high homology with DNA sequence of the same gene that has been deposited in GenBank, there were differences in severall nucleotide including that in the active domain of the enzyme.AbstrakTanaman sagu merupakan sumber pangan dan energi yang sangat potensial untuk dikembangkan karena merupakan tanaman penghasil karbihidrat tertinggi. Pemuliaan tanaman sagu mestinya diarah-kan untuk menghasilkan bibit sagu yang selain memiliki rendemen pati tinggi, juga memiliki diameter empulur besar, tidak berduri dan memiliki cita rasa pati yang enak. Namun, sampai saat ini riset mengenai sagu di Indonesia masih sangat terbatas, sehingga pemuliaan sagu untuk menghasilkan bibit unggul demikian akan memerlukan waktu lama. Ketersediaan penanda rendemen pati akan sangat membantu mempercepat pemuliaan tanaman tersebut. ADP-Glucose Pyrophosphorylase adalah salah satu enzim yang berperan penting dalam biosintesis pati, sehingga gene penyandinya merupakan subjek yang menarik dalam pengembangan marka kandungan pati tinggi. Sebagai bagian dari upaya untuk mendapat-kan penanda rendemen pati tinggi pada tanaman sagu, penelitian ini bertujuan untuk mempelajari ekspresi gen penyandi AGP. RNA diisolasi dari daun tanaman sagu rendemen pati rendah dan tanaman sagu rendemen pati tinggi. Perbedaan tingkat ekspresi gen penyandi AGP dari tanaman sagu rendemen pati tinggi vs rendemen pati rendah, dianalisis dengan teknik Reverse-Transcryptase PCR menggunakan primer spesifik. Hasil penelitian menunjukkan bahwa tanaman sagu rendemen pati tinggi mengekspresikan AGP lebih tinggi dibandingkan dengan tanaman sagu rendemen pati rendah. Ekspresi gen tersebut pada daun tua (full developing leaf) lebih tinggi di-bandingkan dengan pada daun muda, dan pada empulur tidak dideteksi ekspresi gen tersebut. Daerah penyandi lengkap AGP subunit kecil telah diklon. Meskipun memiliki homologi yang tinggi dengan sekuen DNA gen yang sama yang telah dideposit pada GenBank, namun terdapat perbedaan beberapa nukleotida termasuk pada daerah domain aktif dari enzim tersebut.

Filogenetik 5 kultivar sagu dan spesies palmae lainnya berdasarkan penanda molekuler Mat-K Wibawati, Zarima; Abbas, Barahima; Mustamu, Yohanis; Mawikere, Nouke Lenda; Noya, Alce Ilona
Cassowary Vol 7 No 1 (2024): Januari
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v7.i1.202

ABSTRACT: Indonesia is one of the largest producers of sago in the world which is rich in genetic diversity. Improving superior sago varieties can be done through the plant breeding process to determine their genetic characteristics. Genetic diversity can be determined in several ways, namely morphological markers, molecular markers and cytological markers. Molecular markers can be done using several markers, namely nuclear markers (cell nucleus) and cytoplasmic markers. Chloroplast genome markers consist of rbcL and Mat-K. The gene most widely used in research on plant DNA barcoding throughout the world is the Mat-K gene. Analysis of sequencing results for this gene can use the MEGA program to produce kinship relationships. The method used in this experiment is a descriptive method. Based on the research results, it can be concluded that the resulting nucleotide sequence is 578 bp and has 192 amino acids. The genetic distance between the 5 sago cultivars and other palmae species ranged from 0.000-0.003. The phylogenetic tree shows that there are 2 groups with a boostrap value of 75, where in group 1 there is the species Metroxylon sago isolate Sago 15 which has a close relationship with Metroxylon warburgii when compared to other palmae species

Efek aktivator EM4 dan RJ37 formula turbo neo aktiv terhadap laju pengomposan dan kualitas kompos ampas sagu Boari, Marsalina; Musaad, Ishak; Noya, Alce Ilona; Abbas, Barahima
Cassowary Vol 7 No 1 (2024): Januari
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v7.i1.208

ABSTRACT: This research aims to determine the composting rate and quality of sago dregs compost using EM4 and RJ37 Formula Turbo Neo Aktiv (FTNA) activators. The sago dregs composting process with EM4 and RJ37 FTNA activator treatment has an effect on the composting rate and quality of the sago dregs compost. The research used a single factor Randomized Block Design. Parameters observed include C-Organic content, N, P, K, C/N ratio, plant height, number of leaves and wet weight at harvest. The research results showed that EM4 and RJ37 FTNA with doses of 100 ml and 150 ml could accelerate the composting rate of sago dregs for 8 weeks so as to obtain compost quality according to the SNI 19-7030-2004 criteria

Daya hasil genotipe jagung merah Unipa pada beberapa ame-lioran di Distrik Manokwari Utara Lenda Mawikere, Nouke; Kilala, Ary; Ilona Noya, Alce; S. Sarungallo, Amelia; Purbokurniawan; Abbas, Barahima
Cassowary Vol 7 No 2 (2024): Juni
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v7.i2.222

ABSTRACT: This research aims to find out the types of ameliorant and the local West Papua genotypes that have the best influence on corn productivity, identify the yield of several promoting genotypes of the local West Papua red corn using several types of ameliorant in North Manokwari District, and know the right type of the ameliorant for increasing the yield of several promoting genotype of the local West Papua red corn. The study used a split-plot design with the combination treatments of ameliorant types and corn plant genotypes. The types of ameliorant as the first treatment factor which was allocated as a main plot consisted of Control or without ameliorant (Ao), Chicken Manure (A1), and Goat Manure (A2). The second factor (G) as a subplot consisted of 8 types of corn genotypes (AMP-UNIPA 1, AMP-UNIPA 2, AMP-UNIPA 3, AMP-UNIPA 4, AMP-UNIPA 5, AMP-UNIPA 6, AMP-UNIPA 7, and AML-UNIPA (local varieties). The results showed that the combination of ameliorant and corn genotypes treatments produced a different influence on the growth and yield components of corn. Several important variables in the growth component as well as the corn yield component showed the best results as an influence of goat manure ameliorant application.

Ekspresi dan kloning gen penyandi ADP-Glucose Phyrophosphorylase dari tanaman sagu (Metroxylon sagu Rottb.) Expression and cloning of gene encoding ADP-Glucose Phyrophosphorylase from sago palm (Metroxylon sagu Rottb.) Asmini BUDIANI1; Riza Arief PUTRANTO; Hayati MINARSIH; Imron RIYADI; . SUMARYONO; Barahima ABBAS
Menara Perkebunan Vol. 83 No. 2: 83 (2), 2015
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v83i2.4

AbstractSago palm (Metroxylon sagu Rottb.) is a potential food and energy resources becouse it is the highest starch producing plant. Breeding of sago palm should be directed to produce elite genotype with superior characters such as high starch content, wider pith diameter, without spine and high starch quality. However, research on sago palm in Indonesia so far is limited espescially in the field of cultivation and breeding, and attempt to produce such elite would take long time. Availability of molecular marker for starch content would be beneficial to shorten the length period of breeding. ADP-Glucose Phyrophosphorylase is one of the important enzymes in starch biosynthesis. Therefore its gene is an interesting subject in order to develope molecular marker of high starch content. This research was aimed to study the expression of gene encoding AGP in the sago palm with high starch content versus low starch content, and to clone the full cds of the gene. RNA was isolated from leaf and pith of both palms. Exspression analysis and amplify-cation of full cds were conducted by Reverse Transcryptase-Polymerase Chain Reaction (RT-PCR) using specific primers. The results showed that sago palm with higher starch content expressed AGP higher than that of sago palm with lower starch content. Expression of AGP in the full developing leaf was higher than in the young leaf, and there was no expression detected in the pith. The full cds of AGP was successfully amplified and cloned. Even though the DNA sequence showed high homology with DNA sequence of the same gene that has been deposited in GenBank, there were differences in severall nucleotide including that in the active domain of the enzyme.AbstrakTanaman sagu merupakan sumber pangan dan energi yang sangat potensial untuk dikembangkan karena merupakan tanaman penghasil karbihidrat tertinggi. Pemuliaan tanaman sagu mestinya diarah-kan untuk menghasilkan bibit sagu yang selain memiliki rendemen pati tinggi, juga memiliki diameter empulur besar, tidak berduri dan memiliki cita rasa pati yang enak. Namun, sampai saat ini riset mengenai sagu di Indonesia masih sangat terbatas, sehingga pemuliaan sagu untuk menghasilkan bibit unggul demikian akan memerlukan waktu lama. Ketersediaan penanda rendemen pati akan sangat membantu mempercepat pemuliaan tanaman tersebut. ADP-Glucose Pyrophosphorylase adalah salah satu enzim yang berperan penting dalam biosintesis pati, sehingga gene penyandinya merupakan subjek yang menarik dalam pengembangan marka kandungan pati tinggi. Sebagai bagian dari upaya untuk mendapat-kan penanda rendemen pati tinggi pada tanaman sagu, penelitian ini bertujuan untuk mempelajari ekspresi gen penyandi AGP. RNA diisolasi dari daun tanaman sagu rendemen pati rendah dan tanaman sagu rendemen pati tinggi. Perbedaan tingkat ekspresi gen penyandi AGP dari tanaman sagu rendemen pati tinggi vs rendemen pati rendah, dianalisis dengan teknik Reverse-Transcryptase PCR menggunakan primer spesifik. Hasil penelitian menunjukkan bahwa tanaman sagu rendemen pati tinggi mengekspresikan AGP lebih tinggi dibandingkan dengan tanaman sagu rendemen pati rendah. Ekspresi gen tersebut pada daun tua (full developing leaf) lebih tinggi di-bandingkan dengan pada daun muda, dan pada empulur tidak dideteksi ekspresi gen tersebut. Daerah penyandi lengkap AGP subunit kecil telah diklon. Meskipun memiliki homologi yang tinggi dengan sekuen DNA gen yang sama yang telah dideposit pada GenBank, namun terdapat perbedaan beberapa nukleotida termasuk pada daerah domain aktif dari enzim tersebut.

Kloning dan karakterisasi daerah promoter gen penyandi ADP glucose pyrophosphorylase dari Metroxylon sagu rendemen pati-tinggi dan -rendah [Cloning and characterization of promoter region of ADP glucose pyrophosphorylase-encoding gene from Metroxylon sagu with high- and low-starch content] Asmini BUDIANI; Riza Arief PUTRANTO; Hayati MINARSIH; Imron RIYADI; . SUMARYONO; Barahima ABBAS
Menara Perkebunan Vol. 84 No. 1 (2016): 84 (1), 2016
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v84i1.200

ADP-glucose pyrophosphorylase (AGPase) is one of the key enzymes in the starch biosynthesis. In many plants, the activity of this enzyme was reported to affect the yield and composition of the produced starch. This research is a part of an effort to develop molecular markers for early selection of high starch-yielding of sago palm. The purpose of the research was to isolate promoters of AGP gene and to analyze the differences in their DNA sequences between sago palm with high starch content (MsHS) and low starch content (MsLS). DNA was isolated and purified from the leaves of the two sago palm. The promoter region of AGP was amplified by Genome Walking technique. The specific primers were designed by Primer3 program based on the information of DNA sequence of AGP genes of sago palm from previous studies. Selected DNA fragments resulted from Genome Walking were isolated from the gel, cloned into E. coli, and analyzed its DNA sequence. DNA sequence analysis showed that one DNA fragment from MsHS (± 1500 bp) and one DNA fragment from MsLS (> 2000 bp) were confirmed as a 5’ upstream of the AGP gene. Further in silico analysis using MEME program identified various DNA motifs of cis-acting elements, which confirmed that those DNA fragment were promoter region of the gene. Preliminary analysis showed the differences in DNA sequences and motives of cis-acting elements in the promoter region of the two samples which might influence or indirectly associated with the character of the starch yield in sago palm.

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