Article Per Year (5 Year)
p-Index From 2020 - 2025
1.259
P-Index
This Author published in this journals
All Journal Journal of Science and Technology Research for Pharmacy Life Science Tadwin: Jurnal Ilmu Perpustakaan dan Informasi Jurnal Administrasi Karya Dharma International Journal of Cell and Biomedical Science
Prasetio, Ardi
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Library & Information Science Medicine & Pharmacology Social Sciences

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : International Journal of Cell and Biomedical Science

 1 
X-Ray Scanning Reduce Soluble Active Molecules of Mesenchymal Stem Cells Putra, Agung; Pasongka, Zenitalia; Widyatmoko, Agus; Prasetio, Ardi; Dirja, Bayu Tirta
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.14

Abstract

Mesenchymal Stem Cells secrete various anti-inflammatory and regenerative SAMs-MSCs that possess immunomodulatory properties and may accelerate wound healing. As a potential agent for therapeutic, SAMs-MSCs should be stable in any condition, including under X-Ray Scanning. The previous study reveal that X-Ray Scanning may induce protein damage. However, the investigation regarding the stability of SAMs-MSCs under X-Ray Scanning is limited, thus this study aimed to investigate the stability of SAMs-MSCs after X-Ray Scanning. Mesenchymal Stem Cell medium was filtrated using the TFF method at 300 and 5 kDa filter cut off and sterilized using 0,1 um syringe. The SAMs-MSCs underwent X-ray Scanning using public Air Port X-Ray twice. The SAMs-MSCs concentration was measured using ELISA. T-test analysis was performed for the statistical analysis with P<0,05. This study revealed that X-ray scanning reduce the concentration of SAMs-MSC. A previous study found that x-ray irradiation may damage protein at 6–18 keV caused by the energy deposited by photoelectrons that are generated by the interaction of X-ray photons and the protein leading to photoelectron-induced damage.
Effect of Time Transport on Mesenchymal Stem Cell Surface Markers: Unveiling the Influence of Cellular Translocation on Cellular Phenotype Wicaksono, Hendi; Prasetio, Ardi; Risky Chandra Satria Irawan; Naufal Ardjivani Arifin
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i4.29

Abstract

Mesenchymal stem cells (MSCs) hold great promise in regenerative medicine due to their ability to differentiate into multiple cell types and promote tissue repair. However, the effect of transportation time on the surface markers of MSCs remains understudied. This study investigated the impact of transportation time on MSC surface markers, specifically CD73, CD90, CD105, and hematopoietic lineage markers. MSCs were isolated from human umbilical cords and cultured. Flow cytometry analysis confirmed the expression of MSC surface markers. The MSCs were then subjected to simulated transportation for different time periods ranging from 0 to 24 hours at 2-8oC. After transportation, flow cytometry was used to analyze the expression of surface markers. The results showed that prolonged transportation time led to a decrease in the expression levels of CD73, CD90, and CD105, which are important markers for maintaining MSC functionality. Additionally, there was an increase in hematopoietic lineage marker expression. These findings suggest that transportation time can compromise the therapeutic potential of MSCs. Further investigation is needed to understand the underlying mechanisms responsible for the observed changes in surface marker expression. Optimization strategies, such as improved temperature control and protective agents, should be considered to mitigate the negative effects of prolonged transportation time. In conclusion, this study highlights the importance of considering transportation time and its impact on MSC surface markers in cellular therapy protocols. Understanding and addressing these effects are crucial for ensuring the quality and effectiveness of MSC-based therapies.
Priming of Mesenchymal Stem Cells for Enhanced Interleukin-10 Secretion via Conditioned Medium from Lipopolysaccharide-Activated Peripheral Blood Mononuclear Cells Prasetio, Ardi; Syafitri, Luthfiana Mifta; Prabowo, Adam; Alif, Iffan; Nurichsan, Aldan
International Journal of Cell and Biomedical Science Vol 2 No 5 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i5.39

Abstract

Background: Mesenchymal stem cells (MSCs) are known for their immunomodulatory properties, particularly their ability to secrete anti-inflammatory cytokines such as interleukin-10 (IL-10). Enhancing the secretion of IL-10 by MSCs could have significant therapeutic potential for treating inflammatory diseases. Objective: This study aimed to prime the secretion of IL-10 by MSCs through the use of conditioned medium (CM) derived from lipopolysaccharide (LPS)-induced peripheral blood mononuclear cells (PBMCs). Methods: MSCs were isolated from Wharton’s Jelly and characterized using flow cytometry and differentiation assays. PBMCs were isolated from human blood samples and stimulated with LPS to produce a pro-inflammatory environment. The conditioned medium from these LPS-induced PBMCs was collected and add to MSCs culture medium in 5% and 7.5%. After 24h and 48h incubation, IL-10 secretion by MSCs was measured using an enzyme-linked immunosorbent assay (ELISA). Results: The results demonstrated that MSCs cultured in the conditioned medium from LPS-induced PBMCs showed a significant increase in IL-10 secretion compared to control conditions in 24h exposure, but not significantly different in 48h. Conclusion: The exposure of conditioned medium from LPS-induced PBMCs may effectively enhances the secretion of IL-10 by MSCs.
Comparison of Two Tangential Flow Filtration Methods in Isolating CD63+/CD9+ Mesenchymal Stem Cell Exosome Putra, Agung; Alif, Iffan; Prasetio, Ardi; Prawitasari, Salindri
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Extracellular vesicles, particularly CD63+/CD9+ Mesenchymal Stem Cell Exosome (MSC-Exo), have emerged as crucial mediators of intercellular communication and potential therapeutic agents, including regenerative medicine and immunomodulation. However, the precise isolation and purification of MSC exosomes pose critical challenges. Tangential Flow Filtration (TFF) has gained recognition as an efficient exosome isolation method, offering scalability and versatility. In this study, we address the pressing need for standardized exosome isolation methods by comparing two distinct TFF-based protocols for isolating CD63+/CD9+ MSC exosomes based on filter size pore order. Methods: MSC-Exo were conducted from the Stem Cell and Cancer Research Laboratory (SCCR Indonesia), which were then processed through TFF using different filter sizes and orders. There are two filtration methods compared, first, MSC-Exo was filtered with 1000-5-500-300-100-50-10-5 filter order. Second procedure, MSC-Exo was filtered using 1000-500-300-100-50-10-5 filter order. Result: Flow cytometry analysis revealed variations in the percentage of CD63+/CD9+ in the MSC-Exo based on filter order. The results indicate that the choice of filter order significantly influences the size range with the highest concentration of CD63+/CD9+ MSC-Exo. Conclusion: This research underscores the importance of optimizing TFF-based isolation methods for CD63+/CD9+ MSC exosomes, especially in the order of filter pore size.
Co-Authors Agung Putra Agus Widyatmoko, Agus Alif, Iffan Antari, Arini Dewi Dirja, Bayu Tirta Hendi Wicaksono Ipah Ema Jumiati Naufal Ardjivani Arifin Ning Setiati Nugroho, Baskoro Adi Nurichsan, Aldan Pasongka, Zenitalia Prabowo, Adam Prawitasari, Salindri Reviana, Maghfira Nur Risky Chandra Satria Irawan Salsabilla, Diviani Syafitri, Luthfiana Mifta Winanda, Tri