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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Pengendalian Hayati Berkala Ilmiah Pertanian Indonesian Journal of Biotechnology UNEJ e-Proceeding JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ALCHEMY Jurnal Penelitian Kimia Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Proteksi Tanaman Tropis
HARDIAN SUSILO ADDY
Faculty Of Agriculture,University Of Jember, Jl. Kalimantan No. 37, Jember 68121
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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PENGARUH SUMBER MINERAL TERHADAP PENEKANAN Erwinia carotovora OLEH PSEUDOMONAS PENDAR-FLUOR SECARA IN VITRO Hardian Susilo Addy .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 2 (2007): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.84 KB) | DOI: 10.23960/j.hptt.27117-124

Abstract

Antimicrobial Stimulation of Fluorescent Pseudomonad to Inhibit Soft-rot Pathogen Caused by Erwinia carotovora subsp. Carotovora. This research was conducted to study effect of mineral sources on inhibition Erwinia carotovora by fluorescent pseudomonad. We used several mineral sources to stimulate antimicrobial substances from fluorescent pseudomonad that responsible to inhibit E. carotovora subsp. carotovora in vitro. The results showed that zinc 0,5 mM were the best to increase antagonistics of fluorescent psudomonad againts E. carotovora. Zinc were increased antimicrobial substances twohold compared with control without stimulant agent. Detection of antimicrobial substance using TLC showed that only one antimicrobial was detected with retention factor (Rf) of 0,68 – 0,72. However, identification and characterization of that substance is still needed.
Pengaruh Sumber Karbon Terhadap Daya Antagonistik Bakteri Pseudomonas Pendar Fluor Terhadap Erwinia Carotovora Hardian Susilo ADDY
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.794 KB)

Abstract

This research was conducted to study antimicrobial stimulation of fluorescentpseudomonad to inhibit Erwinia carotovora. We used several carbon sources to stimulateantimicrobial substances from fluorescent pseudomonad that available to inhibit E.carotovora in vitro.The results shown that mannitol 10% was the best stimulant agent to increase antagonistics offluorescent psudomonad againts E. carotovora. Also, mannitol increased antimicrobial substancestwohold compared with control without stimulant agent. Detection of antimicrobial substance usingTLC showed that only one antimicrobial was detected with retention factor (Rf) of 0,68. However,identtification and characterisation of that substance is needed.
Aktivitas Pseudomonas Pendar Fluor Dalam Mengendalikan Penyebab Penyakit Patik (Cercospora nicotianae) Pada Tembakau Hardian Susilo Addy
Jurnal Pengendalian Hayati Vol 1 No 2 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

This study was conducted for tenth month in laboratory and greenhouse of Plant protectionDepartment of Agriculture Faculty Jember university. This research was coonducted by two step invitro including identification and screening in laboratory,and in vivo including induce systemicresistant and leaf interaction of antagonist against patogen. The result show that activities offluorescent pseudomonad in vitro against C. nicotianae was by production antibiotic, proteases andsiderophore. Antagonistic invitro was effective under 20-30OC temperatures. Influence of ironconcentration at 5, 50 and 100 μM was affect on antagonist by siderophore, more high concentrationless ability to suppress C. nicotianae growth. In greenhouse test, all isolate was shown ability tocontrol C. nicotianae by induce plant resistant and by antibiosis activities.
Karakterisasi fisiologis dan biokimia penyebab penyakit bakteri pembuluh kayu pada tanaman cengkeh (Syzygium aromaticum L.) di PT Tirta Harapan Mohammad Khoirul Amrulloh; Hardian Susilo Addy; Wiwiek Sri Wahyuni
Jurnal Proteksi Tanaman Tropis Vol 2 No 1 (2021): January
Publisher : Department of Plant Protection, Faculty of Agriculture, Universitas Jember - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jptt.v2i1.17919

Abstract

Bacterial clove wood vessel disease is one of the diseases that affect clove production. The symptoms of the attack are characterized by yellowing of the leaves which then fall, branches and dead branches followed by sudden wilting with insect vectors namely Hindola sp. BPKC disease in 1990 was initially identified as Pseudomonas syzygii, but in 2004 it was re-identified as Ralstonia syzygii. The research method used is physiological characterization and biochemical characterization. The results of physiological characterization in the form of gram test, hypersensitivity test, NaCl level test and growth have the exact same character as the existing literature, while the results in biochemical characterization are glucose, sucrose, maltose, mannitol, dulsitol and sorbitol. only different in the dulsitol and sorbitol test. Keywords: characterization, bacterial wilt, identification
Pengaruh Konsentrasi Asam Terhadap Sifat Fisik dan Muatan Permukaan Selulosa Termodifikasi Agus Wedi Pratama; Bambang Piluharto; Dwi Indarti; Tanti Haryati; Hardian Susilo Addy
ALCHEMY Jurnal Penelitian Kimia Vol 15, No 2 (2019): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.15.2.33756.315-328

Abstract

Selulosa merupakan salah satu biopolimer melimpah yang banyak digunakan dalam berbagai bidang seperti kertas, energi dan material komposit. Hidrofilisitas, dapat diperbaharui, ramah lingkungan dan aman adalah sifat-sifat selulosa yang dapat berpotensi menjadi material maju. Berdasarkan sifat-sifatnya, selulosa dapat dimodifikasi untuk menghasilkan sifat fungsional yang sesuai dengan aplikasinya. Dalam penelitian ini, selulosa mikrokristalin (MCC) dimodifikasi melalui metode hidrolisis asam. Prinsip metode ini adalah penghilangan bagian amorf pada selulosa oleh asam, meninggalkan bagian kristal. Selain itu, ketika asam digunakan sebagai agen hidrolisis, maka akan menghasilkan muatan permukaan pada selulosa. Dalam penelitian ini, pengaruh berbagai konsentrasi asam pada struktur kimia, kristalinitas, morfologi dan muatan permukaan telah dikaji. Perubahan struktur selulosa dianalisis menggunakan Fourier Transform Infra Red (FTIR), kristalinitas menggunakan X-ray Diffraction (XRD), morfologi menggunakan Scanning Electron Microscopy (SEM) dan muatan permukaan menggunakan titrasi konduktomteri. Hasil analisis FTIR menunjukkan masuknya gugus sulfat pada struktur selulosa. Analisis XRD menunjukkan peningkatan kristallinitas dalam selulosa termodifikasi seiring bertambahnya konsentrasi asam. Hasil analisis morfologi menunjukkan partikel dalam selulosa termodifikasi (CM) lebih tersebar daripada MCC. Analisis titrasi konduktometri menunjukkan bahwa mengalami peningkatan muatan permukaan pada CM seiring dengan bertambahnya konsentrasi asam. Oleh karena itu, dapat disimpulkan bahwa pengaruh konsentrasi asam sulfat pada hidrolisis selulosa memberikan dampak yang signifikan pada sifat fisik dan muatan permukaan.Effect of Acid Concentration on Physical Properties and Surface charge of Modified Cellulose. Cellulose is one of abundant biopolymer that many widely used in various applications such as paper, energy and composite material. Hydrophilicity, renewable, biodegradable, and safety are cellulose properties that can became potential of advance materials. In the utilization, cellulose can be modified its properties for different purposes. In this work, microcrystalline cellulose (MCC) was modified by acid hydrolysis method. The principle of this method is removed amorphous region of cellulose by acid and leaving crystalline phase. Moreover, when acid was used as hydrolyzing agent, it produce the surface charge on cellulose. In this research, the effect of various concentration of acid on the chemical structure, crystallinity, morphology and surface charge have studied. The chemical structures were analyzed using Fourier Transform Infra Red (FTIR), crystallinity using X-ray Diffraction (XRD), morphology using Scanning Electron Microscopy (SEM), and surface charge using titration conductometric. The FTIR analysis result has successfully showed the entry of sulfate groups on the cellulose structure. The XRD analysis showed increasing crystallinity in Cellulose Modified (CM) with increase acid concentration. By morphology analysis, particles in CM more disperse than MCC. Analysis of conductometric titration shows that there is an increase in surface charge in CM as acid concentration increases. Thus, the effect of sulfuric acid concentration on hydrolysis of cellulose has a significant impact on physical properties and surface charge.
Insidensi dan Keparahan Penyakit Penting Tanaman Padi di kabupaten Jember Rachmi Masnilah; Wiwiek Sri Wahyuni; Suhartiningsih Dwi N; Abdul Majid; Hardian Susilo Addy; Ali Wafa
AGRITROP Vol 18, No 1 (2020): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v18i1.3103

Abstract

Jember merupakan salah satu Kabupaten penghasil padi terbesar di jawa timur, Indonesia. Sayangnya berdasarkan Badan Pusat Data Statistik (BPS) pada tahun 2015-2016, produksi padi di Kabupaten Jember Menurun hingga 20.000 ton per tahun. Masalah hama dan penyakit menjadi penyebab utama turunnya produktifitas padi di Jember. Di ketahui tanaman padi di Jember dapat diserang oleh beberapa penyakit dalam kurun waktu yang sama dengan insidensi dan keparahan yang berbeda. Penelitian dilakukan guna mengetahui tingkat insidensi dan keparahan penyakit penting di beberapa kecamatan di Kabupaten Jember. Lokasi pengambilan data diperoleh berdasarkan metode pengacakan bertingkat dan dilakukan di musim kering. Sampel diambil dari empat desa per kecamatannya dan per desa diambil minimal empat titik lahan. Hasil menunjukkan Beberapa penyakit diketahui menyerang semua lokasi penyakit seperti Penyakit hawar bakteri (Kresek) dan Blast. Umumnya masing-masing kecamatan berbeda jenis penyakit yang menyerang. Sebagai contoh Tungro dan penyakit lain yang diebabkan oleh virus hanya menyerang pada beberapa lokasi dengan insidensi dan keparahan yang kecil. Data hasil penelitian ini dapat digunakan untuk dasar peramalan terjadinya epidemi penyakit di kemudian hari dan mempersiapkan metode pengendalian penyakit tanaman padi yang bijaksana pada musim yang sama.
EKSPLORASI Bacillus spp., DARI PERAKARAN KUBIS SEBAGAI AGEN ANTAGONIS Xanthomonas campestris pv. campestris Fajar Dwi Agustina Wati; Suhartiningsih Dwi Nurcahyanti; Hardian Susilo Addy
AGRITROP Vol 15, No 2 (2017): Agritrop : Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agr.v15i2.1178

Abstract

Isolasi Bacillus spp diambil dari tanah rhizosfer kubis sehat di oven dengan suhu 80○C selama 1 jam dan ditumbuhkan pada media YPGA.Sebanyak 24 isolat Bacillus spp merupakan gram positif dan hipersensitif bersifat negatif. Isolat tersebut dilakukan uji zona penghambatan terhadap Xanthomonas campestris pv. campestris, terdapat 12 isolat yang membentuk zona hambatan. Ke 12 isolat Bacillus spp tersebut memiliki perbedaan ukuran daam membentuk zona hambatan dan memiliki mekanisme penghambatan bakteriostatik, isolat tersebut masing-masing diambil 2 perwakilan untuk kategori zona hambatan besar, sedang dan kecil. Terdapat 6 isolat terpilih, kemudian dilakukan karakterisasi yang terdiri dari uji oksidase, katalase, dan hidrolisa pati dan memiliki kesamaan dalam hasil karakeristiknya.
Resistance gene expression in selected Indonesian pigmented rice varieties against infection by Xanthomonas oryzae pv. oryzae Nur Elia Nadhira; Ali Wafa; Wahyu Indra Duwi Fanata; Hardian Susilo Addy
Indonesian Journal of Biotechnology Vol 27, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.70445

Abstract

Rice (Oryza sativa L.) production is limited by bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo). For decades, researchers have attempted to control this disease by growing plants with blight‐resistant Xa genes. Genetic resources often vary between rice varieties, and there is little information about the genetic resources of the pigmented rice varieties widely grown in Indonesia and their resistance genes against Xoo. The purpose of this study was to determine the expression of Xa genes in pigmented rice such as Inpari 24 and Cempo Merah (red‐pigmented) along with Hitam Bantul (black‐pigmented) and white rice varieties IR64 and Ciherang, and to evaluate their resistance to BLB. All varieties carried the Xa4, Xa10 and xa13 genes but varied in the Xa1, Xa7 and Xa21 genes. The rice varieties expressed some of these genes only after inoculation with Xoo. Disease assessment categorised the three different pigmented rice varieties as resistant (Ciherang, Cempo Merah and Hitam Bantul), while IR64 (white) and Inpari 24 (red) were moderately resistant. There was no specific pattern of Xa genes possession, quality of expression or resistance level to X. oryzae pv. oryzae. Therefore, when breeding plants, the selection of parental variety must be considered in terms of the possession and expression of Xa genes such as Xa10 as a molecular marker for resistance.
Design and construction of single guide RNA for CRISPR/Cas9 system based on the xa13 resistance gene in some varieties of rice (Oryza sativa) Hardiyani, Wulan Arum; Wafa, Ali; Fanata, Wahyu Indra Duwi; Addy, Hardian Susilo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 1 (2023): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12347-55

Abstract

The xa13 gene is a recessive resistance gene against Xanthomonas oryzae pv. oryzae (Xoo) found in several rice varieties. Activation of this gene will trigger the formation of sucrose as a nutrient supply to Xoo for their growth in the plant. The disruption of this recessive gene expression in the plant can affect the negative impact of the gene, and recently can be created using clustered regularly interspaced short palindromic repeats (CRISPR) system using CRISPR-associated protein-9 (CRISPR/Cas9) technology that requires gRNA to recognize the targeted-sequence. This study aimed to design and construct the gRNA-targeting xa13 gene in rice using bioinformatics tools. CHOPCHOP was used for generated the gRNA candidates according to the target gene sequence. Two candidates of gRNA-targeted xa13 have been selected based on the analysis of bioinformatics data. Each candidate of gRNA consisted of 20 nucleotides (nt) of the target sequence upstream 3 nt of the protospacer adjacent motif (PAM) sequence (5’-NGG) targeting two exons in the xa13 gene. The gRNA1 will target exon 1 and the gRNA2 will target exon 2, with an efficiency of 52.51% and 44.63% respectively. Data showed that the GC content of all gRNA candidates ranged from 55–70% with no target-off location in the whole genome of rice. The transformation and confirmation test based on the physiological and genomic characteristics of transformants confirmed that the design has been successfully constructed.
The Metarhizium anisopliae Metsch. (Moniliales: Moniliaceae) and Aglaia odorata Lour. (Sapindales: Maliaceae) leaf extract: a potent biopesticide cocktail for cabbage leaf caterpillar management Prastowo , Sigit; Wardhani, Widi Kusuma; Pradana, Ankardiansyah Pandu; Wafa, Ali; Addy, Hardian Susilo; Hadi, Zul Fauzi Nugroho
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 2 (2024): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.224212-222

Abstract

Plutella xylostella Linnaeus (Lepidoptera: Plutellidaae) is an important pest on Brassicaceae plants, especially cabbage. The combination of entomopathogenic fungi and botanical pesticides has been widely developed to increase effectiveness effectiveness against P. xylostella . This research aimed to determine the potential of Metarhizium anisopliae and Aglaia odorata Leaf Extract (Ao-LE) as a combined control strategy for P. xylostella. The parameters observed in this research were colony growth, viability of conidia of M. anisopliae, antifeedant activity, mortality, and lethal time 50 (LT50) of P. xylostella . The findings revealed that Ao-LE addition maintained colony growth comparable to that of the control treatment, while significantly enhancing the conidial viability of M. anisopliae. Notably, 0.80% and 1.00% Ao-LE in the M. anisopliae suspension resulted in maximum antifeeding activity. Moreover, incorporating 0.20-1.00% Ao-LE demonstrably improved P. xylostella mortality compared with M. anisopliae alone. The combination of M. anisopliae 107 conidia/mL and 1.00% Ao-LE exhibited the lowest LT50 value of 2.16 days, highlighting the synergistic effect of the combined treatment. In conclusion, this study provides compelling evidence for the compatibility and synergistic potential of M. anisopliae and Ao-LE, presenting a promising strategy for the sustainable management of P. xylostella infestations.
Co-Authors .,, Rasmiyana Abdul Majid Abdul Majid Agus Wedi Pratama Agustina Wati, Fajar Dwi Ali Wafa Ankardiansyah Pandu Pradana Ariestya Ayu Meda Sallytha Ariestya Ayu Meda Sallytha Bambang Piluharto Bambang Sugiharto BUDI ARMAN Budi Kriswanto Budi Kriswanto, Budi Dwi Indarti Erlia Narulita Fajar Dwi Agustina Wati Fanata, Wahyu Indra Duwi Hadi, Zul Fauzi Nugroho Hardiyani, Wulan Arum Mohammad Khoirul Amrulloh N, Suhartiningsih Dwi Nur Elia Nadhira Nurul Rama Dhany Paniman Ashna Mihardjo Paniman Ashna MIHARDJO Prastowo , Sigit Rachmi Masnilah Restanto, Didik Pudji Retnosari Apriasti Slameto . Slameto Slameto Suhartiningsih Dwi N Suhartiningsih Dwi Nurcahyanti Tanti Haryati TRI CANDRA SETYOWATI Tri Handoyo TRI HANDOYO Wahyu Indra Duwi Fanata Wardhani, Widi Kusuma Weny Nailul Hidayati WIWIEK SRI WAHYUNI