Ida Bagus Windia Adnyana
Fakultas Kedokteran Hewan Universitas Udayana

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Journal : Jurnal Veteriner

The Differences of Ultrasonography Imaging Between Green Turtles (Chelonia mydas) and Olive Ridley Turtles (Lepidochelys olivacea) in Bali (PERBEDAAN CITRA ULTRASONOGRAFI ANTARA PENYU HIJAU (CHELONIA MYDAS) DAN PENYU LEKANG (LEPIDOCHELYS OLIVACEA) DI BALI I Wayan Nico Fajar Gunawan; Archie Leander Maslim; Ida Bagus Windia Adnyana; Anak Agung Gde Oka Dharmayudha; Luh Made Sudimartini
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.388 KB)

Abstract

Ultrasonography is a rapid and non-invasive method for assessing internal organs. The purpose of this study is to determine the differences in ultrasonographic imaging of internal organs between green turtle (Chelonia mydas) and olive ridley turtles (Lepidochelys olivacea). This study used two female green turtles (C. mydas) and two olive ridley turtle (one male and one female) (L. olivacea) with body condition index ranging from Average-Very Good. Micro-convex transducer ultrasonography with frequencies 4.5-8 MHz was used in this study. Acoustic windows were used in this study from cervical dorsal, cervical ventral, cervicobrachial, sinister-dexter, axillary sinister-dexter, prephemoral sinister-dexter and postphemoral sinister-dexter. The necropsies were performed to provide reference data. The results of ultrasonography imaging showed that the jugular vein of the olive ridley turtles (L. olivacea) was wider than the jugular vein of the green turtles (C. mydas). The ultrasonography imaging also showed that the stomach of green turtles (C. mydas) was smooth folds but the stomach of olive ridley turtles (L.olivacea) was sharp folds according to necropsy. There were no differences in the heart, stomach, liver, small intestine, large intestine, and kidneys. Vitellogenic follicles ultrasonography imaging was found from the olive ridley turtles (L. olivacea) which had shown in the period of premating and mating.
Studi Kasus: Ascariosis Disertai Migrasi Larva pada Hati dan Paru-paru Babi Landrace (CASE STUDY: ASCARIOSIS ACCOMPANIED BY MIGRATION OF LARVAE IN LIVER AND LUNGS OF LANDRACE PIG) I Ketur Berata; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Made Kardena; Ida Bagus Windia Adnyana
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.797 KB) | DOI: 10.19087/jveteriner.2019.20.4.603

Abstract

Cases of ascariosis due to infection with Ascaris suum are still prevalent in the world, including Indonesia. Apart from causing economic losses, ascariosis can also threaten human health because it is zoonotic. Case studies of ascariosis with typical white spot lesions in the landrace pig liver is described in this paper. Pigs aged 60 days old, sick for 10 days, thin and coughing gathered from the village of Suwung, Denpasar City. After necropsy and post mortem examination were done, tens of A. suum worms were found in the intestine and white spot lesions observed on the livers. Tissues of small intestine, large intestine, liver, lungs, kidney, spleen, urinary vesicles and brain were taken and put into 10% of formalin neutral buffer before they then processed in making histopathological preparations. Histopathological preparation was carried out using paraffin block method and hematoxylin eosin (HE) staining technique. The results of histopathological examination of the small intestine, large intestine, liver and lungs were found to be infiltrated by eosinophil cells. Eosinophil cells infiltration indicates that there are parasites A. suum or the larvae migrant in the tissues. Some of the tissues in livers were also found to have fibrosis, which is suspected that the infection has been chronic. It can be concluded that cases of the landrace pigs were chronically infected by A.suum and accompanied by migration of the larvae to the livers and lungs. More attention is needed to prevent the increase of ascariosis in pigs in order to minimize economic losses and transmission to humans.
Perubahan Patologi Sistem Hepatobiliari Sapi Bali Terinfeksi Fasciola gigantica Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; I Made Kardena; Ida Bagus Windia Adnyana; Ida Bagus Kade Suardana
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.416 KB) | DOI: 10.19087/jveteriner.2020.21.4.654

Abstract

Liver fluke infections is an important parasitic disease that common attack cattle, buffalo and others ruminant. The disease in the tropics is caused by Fasciola gigantica trematodes. Fasciolosis found in Bali cattle besides causing high economic loss also has the opportunity infect humans. This study aims to determine the pathology changes of Bali cattle hepatobiliary system infected by F. gigantica. In January2019 were examined a hundred of liver tissue Bali cattle. Based on the sex as many as 75 bali cattle are cows and 25 are bulls. On macroscopic examination was found fifteen bali cattles in gallbladder containing of F. gigantica. In positive Fasciolosis the surface of liver look uneven with enlargement of lymph nodes. The tissue of Bali cattle liver infected with F. gigantica is cut into 1x1x1 cm and put in a pot that has been filled with 10% neutral buffered formaline. The fixed tissue then processed in a tissue processor and stained with hematoxylin-eosin (HE). Microscopic examination was found bile ducts proliferation and blocked of the lumen accompanied by cholangio cirrhosis. Found longitudinal pieces of Fasciola giganticaliver fluke surrounded by fibrous connective tissue and multifocal coagulative necrosis with fibroblast proliferation. In some location the infiltration of neutrophil cells around the bile ducts can also be seen. Congestion, bleeding, and neutrophil infiltration are also seen in areas of necrosis. In the bile duct wall bleeding, adenomatus hyperplasia, infiltration of mononuclear cells with mild intensity and foci ofcalcification are found. It can be concluded that there was a change with severe intensity accompanied by chronic inflammation in the hepatobiliary system of Bali cattle with Fasciolosis.
Gambaran Darah Penyu Hijau (Chelonia mydas) Dengan Atau Tanpa Fibropapillomatosis Kadek Intan Dwityanti Devi; Ida Bagus Windia Adnyana; I Gede Soma
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.661 KB) | DOI: 10.19087/jveteriner.2021.22.2.278

Abstract

Penyu merupakan salah satu hewan yang keberadaannya terancam punah. Di Bali temuan kasus penyelundupan penyu sering terjadi, beberapa dari penyu hijau ini mengalami fibropapillomatosis (FP) yang merupakan tumor yang ditemukan pada semua spesies penyu, tetapi paling banyak ditemukan pada penyu hijau. FP ditandai dengan epizootic tumor kulit, flippers, jaringan periokular, karapas, dan plastron. Pemeriksaan hematologi menjadi metode diagnostik, dengan melihat adanya kenaikan atau penurunan jumlah sel darah. Penelitian ini menggunakan sampel penyu hijau dari sitaan Polsek Kuta dengan jumlah penyu hijau dengan fibropappilomatosis 5 ekor dan tanpa fibropapillomatosis 5 ekor. Pengumpulan data pada penelitian ini dilakukan dengan pemeriksaan hematologi. Pemeriksaan hematologi meliputi total eritrosit, nilai hematokrit, dan kadar hemoglobin. Data yang telah diperoleh dianalisis dengan analisis kuantitatif dan dijelaskan secara deskriptif. Hasil gambaran darah penyu hijau dengan fibropapillomatosis dengan rata-rata total eritrosit sebesar 0,55 x 106/µL ± 0,17, PCV 31,4% ± 2,3, Hb 7,2 g/dL ± 1, serta indeks eritrosit, meliputi MCV 616,7 fl ± 176,8, MCH 140,4 pg ± 31,3, MCHC 23,1 g/dL ± 1,9. Gambaran darah dari penyu hijau tanpa fibropapillomatosis memiliki rata-rata total eritrosit 0,46 x 106/µL ± 0.18, PCV 29% ± 0,7, Hb 7,1 g/dL ± 0,7, serta indeks eritrosit meliputi MCV 784,7 fl ± 495,1, MCH 191,9 pg ± 123,8, MCHC 24,4 g/dL ± 1,9. Tidak ada perbedaan yang signifikan terhadap gambaran darah penyu hijau dengan atau tanpa fibropapillomatosis.
Hematological Profiles of Green Sea Turtle (Chelonia mydas) Rehabilitated at Turtle Conservation and Education Center, Serangan Island, Denpasar, Bali Leoni Arifah Agustina; Nyoman Sadra Dharmawan; Ida Bagus Windia Adnyana
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.066 KB)

Abstract

Threat of extinction results in the increasing sea turtle conservation efforts through scientific research in recent years. During rehabilitation process, the non-invasive blood analysis is considered to be highly valuable to determine sea turtle’s health. The objective of this study was to assess the hematological profiles of green sea turtles (Chelonia mydas) under rehabilitation at Turtle Conservation and Education Center (TCEC) Serangan Island, Denpasar, Bali. Three mililiters of peripheral blood from healthy green sea turtles were collected from cervical dorsal sinuses and kept in heparinized vacutainer tube before proceeded with hematological evaluation. PCV was determined by microhematocrit method. Diff count and thrombocyte percentage were determined using Giemsa-stained blood smear under the microscope. RBC and WBC total count were determined using hemocytometer. Hemoglobin (Hb) value was determined using Sahli method. Each hematological values were then analyzed using descriptive analysis. The correlation between body condition index (BCI) and hematological values were analyzed using Spearman’s rank correlation test. The mean values evaluated was 7.28 g/dL in Hb, 31.35% in PCV, 0.67 x 106/?L in total RBC count, 479.37 fl in MCV, 111.38 pg in MCH, 23.42 g/dL in MCHC, 4.01 x 103/?L in total WBC count, 1.20 x 103/?L in lymphocyte, 0.21 x 103/?L in monocyte, 0.10 x 103/?L in eosinophil, 2.48 x 103/?L in heterophil and 0.01 x 103/?L in basophil. No significant correlation was found between the BCI and hematological profiles evaluated.
Dermatitis Suppurative Mengikuti Infeksi Tungau Demodeks dan Sarcoptes pada Anjing Kampung di Jalanan Kota Denpasar Ida Bagus Oka Winaya; Ida Bagus Windia Adnyana; I Ketut Berata; Ida Ayu Pasti Apsari
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.328

Abstract

Tujuan penelitian ini adalah untuk mengetahui penyebab dermatitis dan kerontokan rambut pada anjing jalanan di Kota Denpasar. Dilakukan pencatatan terhadap identitas 75 ekor anjing yang meliputi umur, ras, tipe rambut dan lokasi lesi pada permukaan tubuh. Sampel biopsi kulit dengan ukuran 6 mm diambil dari lesi kulit yang paling parah dengan tanda klinis hiperemia, gatal, adanya krusta, bersisik/scaling, kebotakan/alopecia. Pengambilan sampel biopsi dilakukan dengan terlebih dulu memberikan injeksi anestetik lokal dan dilanjutkan dengan pemberian spraying antiseptik setelah pengangkatan jaringan biopsi. Sampel jaringan kulit kemudian difiksasi dalam larutan neutral buffered formalin 10%. Setelah 24-48 jam dalam cairan NBF jaringan diembedding dalam blok paraffin dengan metode standar. Sedian dengan ketebalan 5 mikron selanjutnya diwarnai dengan hematoksilin-eosin (HE). Pemeriksaan histopatologi terhadap tujuh ekor anjing penderita dermatitis dan kerontokan rambut umur di bawah satu tahun ditemukan kumpulan tungau demodek di beberapa folikel bulu. Eksudat radang netrofil dan eritrosit juga ditemukan pada permukaan epidermis yang nekrosis. Pada pemeriksaan terhadap 26 ekor anjing penderita dermatitis dan kerontokan bulu umur di atas satu tahun ditemukan 24 ekor anjing murni terinfeksi tungau demodeks dan dua ekor terinfeksi tungau demodeks dan sarcoptes. Ditemukan juga adanya larva tungau demodeks di luar folikel rambut. Larva tungau perifolikel terlihat dikelilingi oleh netrofil pada area dermis nekrosis, sedangkan pada anjing yang terinfeksi oleh dua jenis tungau hanya ditemukan respons radang ringan di sekitar folikel rambut.