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Endoparasitic Infestation Case on Buffalo (Bubalus bubalis) in West Praya District of Central Lombok Muhammad Hipzul Mursyid; Anwar Rosyidi; Wayan Wariata; Made Sriasih
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 6 No 1 (2020): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.222 KB) | DOI: 10.29303/jitpi.v5i2.66

Abstract

The purpose of this study was to determine endoparasitic infestation on Buffaloes in Praya Barat district, Central Lombok. Stools samples were taken from 61 buffaloes from 5 villages and examined at Banyumulek Animal Health Laboratory of Animal Husbandry and Animal Health Service of NTB Province using the floating and sedimentation methods to perceive the presence of parasitic eggs. The data obtained were analyzed descriptively. The results show that from 61 fecal samples analyzed, 20 samples (32.7%) were positively infected with endoparasites with a single type of infection and multi-species infection. A total of 13 samples (21.3%) were infected with Nematodes, 2 samples (3.27%) were infected with Cestode, 2 samples (3.27%) were infected with Trematode and 11 samples (18.03%) were infected with Protozoa. The degree of endoparasitic infection in buffaloes in West Praya district is included in the category of mild infection. Endoparasites that infect buffaloes in Praya Barat are coming from family Toxocaridae, Trichostrongylidae, Cooperidae, Anoplocephalidae, Strongylidae, Strongyloididae, Chabertidae, Fasciolidae and Eimeriidae. The highest prevalence for worm parasite was Toxocaridae with the percentage of 11.46%, whilst for the protozoa was the family of Eimeriidae accounted for 18.01%.
Identifikasi Keragaman Genetik Gen Growth Hormon Receptor Dengan Enzim Restriksi Mbo Ii (Ghr|Mbo Ii) pada Sapi Bali Nurul Huda; Made Sriasih; Maskur Maskur
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 1 No 1 (2015): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.407 KB) | DOI: 10.29303/jitpi.v1i1.7

Abstract

Growth Hormone Receptor (GHR) gene is a member of the super familycytokine/hematopoietin receptor. GHR plays a role in mediating the biological activity of growth hormone on target cells that lead to the affection of the nature ofgrowth in beef cattle carcasses and the nature of milk production in dairy cows. This study was designed to identify the genetic diversity of GHR genes exon 10 in Bali cattle. Genotype variation of gene candidates was identified using PCR-RFLP technique. The results showed that mutation at position of 755bp exon 10 of GHR gene as determined by Maskur (2012) can be identified using MBO II restriction enzymes at position of 125bp or at position of 282bp base nucleotidescaused by transition mutation of C/T and substitution of amino acid threonine to isoleucine.Genotype identification of GHR gene exon 10 resulted in three individual genotypes in Bali cattle population namely CC, TT and CT with frequency of 0.1931; 0.5455 and 0.2614respectively. The result of X² test and heterozygosity observationas well as heterozygosity expectation indicate that the genotypedistribution on Bali cattle population in this study were not in Hardy-Weinberg equilibrium (Hardy-Weinberg Equilibrium/ HWE). The value of GHR|MBO II gene Polymorphic Informative Content was 0.342051, might be used for genetic identifier, and was quite informative as gene identifier for linkage analysis in the population.
Analisis Tingkat Pengetahuan, Sikap dan Perilaku Peternak Unggas Terhadap Upaya Pencegahan Penyakit Flu Burung Rusman Effendi; Adji Santoso Dradjat; Made Sriasih
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 2 No 1 (2016): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.527 KB) | DOI: 10.29303/jitpi.v2i1.21

Abstract

The aim of this research was to describe and to correlate knowledge, attitude and practice (KAP) of thepoultry breeders in preventing Avian Influenza (AI). This research used face-to-face interview, cross sectionaldesign study with a questionnaire for 100 respondentsand data were analyzed with Rho Spearman. The resultshowed that 82% of respondents washed their hands with soap after handling poultry but only 5% ofrespondents used mask when contacting with poultry. Chickens were kept by respondents most. The cagelocation near to the house accounted for 71%, most of respondents cleaned the cage routinely but there were11% of respondents who did not keep their poultry into the cage. All respondents revealed that the poultrywere not vaccinated periodically. It was about 37.3% of respondents burned dead poultry, and 55.2% and 22.4% buried and throw away to the river respectively. They disagreed with stamping out accounted for 48% andonly 16% of respondents agreed to sell their unhealthy poultry. No respondents informed to the authority ofsub-village when suddent death of the chickens was found. Respondent’s knowledge, attitude and practicewere suffient in preventing Avian Influenza and there was significant correlation between knowledge, attitudeand practice (P<0,05).
Identifikasi Keragaman Gen Leptin pada sapi Bali dan kambing Kacang Nining Syarifulaya; Made Sriasih; Maskur Maskur
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 1 No 1 (2015): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.699 KB) | DOI: 10.29303/jitpi.v1i1.9

Abstract

The purpose of this research was to identify polymorphism of leptin gene in Bali cattle and Kacang goat by PCR-RFLP technique. Material used was blood samples collected from 50 head of Bali cattle and 50 head of Kacang goat. Identification procedure was consisted of three stages namely extraction of DNA genom, PCR amplication of leptin gene (552bp on intron 2-exon 3) and RFLP method using enzyme restriction (BsaA1). Primer sequences used were forward primer: 5'GTCTGGAGGCA AAGGGCAGAGT-3'and reverse primer: 5'CCACCACCTCTGTGGAGTAG-3'. Data analyzed were frequency of allele, genotype frequency, heterozygosity value, and value of Polymorphic Informative Content (PIC). The results of this study indicate that three genotypes (AA, AG and GG) were found on Bali cattle whilst only two genotypes (AA andAG) were on Kacang goat. The values of heterozygosity observation (Ho) and expectation heterozygosity (He) on Bali cattle were 0.08 and 0.48 respectively. Kacang goat showed the value of 0,14 (Ho) and 0.14 (He). The PIC value of Leptin-BsaA1gene on Bali cattle was 0.43 (moderate high), and on Kacang goat considered as low (0.13). These results showed than Leptin-BsaA1 in Bali cattle and Kacang goat may be used as a genetic marker for selection.
Infection and Distribution of Liver Fluke (Fasciola Sp) Zoonotic Parasite on Cattle in Central Lombok and East Lombok Districts Wayan Wariata; Made Sriasih; Anwar Rosyidi; Muhamad Ali; Sulaiman N. Depamede
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 5 No 2 (2019): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.317 KB) | DOI: 10.29303/jitpi.v5i1.55

Abstract

Salah satu faktor yang sangat mempengaruhi populasi dan produksi ternak adalah masalah penyakit dan parasit. Gejala serangan parasit seringkali tidak tampak dan kadang-kadang petani kurang menyadarinya. Cacing hati (Fasciola sp.) merupakan parasit zoonosis yang dapat menginfeksi berbagai macam hewan ternak ruminansia terutama sapi. Fasciolamemiliki dua spesies utama yaitu hepatica dan gigantica. Penyebaran Fasciola sp. adalah daerah beriklim tropis basah yaitu di Asia dan Afrika. Fasciola sp. yang sering dijumpai di Indonesia adalah spesies gigantica, sedangkan spesies hepatica sering terdeteksi pada sapi-sapi impor. Untuk mengetahui infeksidan tingkat penyebaran Fasciola giganticadan Fasciola hepaticapada ternak sapi di kabupaten Lombok Tengahdan Lombok Timurmaka dilakukan survei, pengamatan dan pengambilan sampel organ hati sapi di pasar-pasar daging yang ada pada dua daerah tersebut. Pada masing-masing daerah diambil 3 lokasi pasar daging, yaitu di kabupaten Lombok Tengah: pasar Praya, pasar Mantangdan pasar Kopang; di kabupaten Lombok Timur: pasarSelong, pasarMasbagikdan pasarTerara. Pada masing-masing pasar, pengamatan dan pengambilan sampel diulang sebanyak 3 kalipada 3 orang pedagang.Selanjutnya sampel diamati di laboratorium untuk mengetahui adanya cacing hati dan kerusakan hati sapi. Hasil penelitian menunjukkan bahwa, sapi-sapi dan daging sapi yang diperjual belikan di pasar-pasar Lombok Tengah dan Lombok Timur terinfeksi parasit cacing hati (Fasciola sp.), dengan tingkat infeksi yang masih rendah, yaitu antara 1,3 sampai 2,3. Di samping itu, warna dan tekstur hati sapi yang diperjual belikan di pasar-pasar Lombok Tengah dan Lombok Timur relatif masih baik dan layak dikonsumsi.
Effects of Adding Vitamin C in Commercial Feed to Reduce Heat Stress in Joper (Java Super) Chickens Kept in Open Cage Beny, Radius; Mohammad Hasil Tamzil; Made Sriasih
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 10 No 1 (2024): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Ani
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mendapatkan informasi mengenai efek penambahan vitamin C dalam pakan komersial sehingga mampu mereduksi stres panas pada ayam Joper. Penelitian dilaksanakan pada bulan Agustus sampai November 2023 di Laboratorium Terapan (Teaching Farm) Fakultas Peternakan Universitas Mataram. Sebanyak 100 ekor ayam Joper dikelompokkan dalam 4 kelompok yaitu P0 (kelompok kontrol); P1-P3 (kelompok dengan pemberian vitamin C masing-masing dengan dosis 100 mg/kgBB, 200 mg/kgBB dan 300 mg/kgBB). Rancangan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) pola searah menggunakan 4 perlakuan dan 5 ulangan. Darah sampel diambil kemudian diperiksa di Laboratorium Dinas Peternakan dan Kesehatan Hewan Nusa Tenggara Barat. Variabel yang diamati adalah gambaran hematologi darah yang meliputi jumlah eritrosit, hemoglobin, nilai hematokrit, leukosit dan diferensiasi leukosit, serta rasio heterofil/limfosit (H/L). Hasil penelitian menunjukkan bahwa tidak adanya pengaruh (P>0,05) dari pemberian vitamin C terhadap gambaran eritrosit, hemoglobin, dan hematokrit. Pemberian vitamin C terhadap total leukosit berpengaruh nyata (P<0,05) Hasil pemeriksaan profil hematologi eritrosit, leukosit, hemoglobin dan hematokrit menunjukkan kondisi normal. Suhu lingkungan yang berkisar antara 26,4°C-31,9°C berpengaruh nyata (P<0,05) terhadap peningkatan rasio H/L. Penambahan vitamin C sebesar 100 mg/kg pakan, 200 mg/kg pakan, 300 mg/kg pakan mampu mengurangi tingkat kerusakan yang diakibatkan oleh stres panas.