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Uji Aktivitas Enzim pada buah Mangga (Mangifera indica L) erwansani, enggy; Reynaldi, Muhammad Andre; Yuswar, Muhammad Akib; Najini, Robby
Journal Pharmacy Of Tanjungpura Vol 2, No 2 (2025): APRIL
Publisher : Universitas Tanjungpura

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Pendahuluan: Mangga tidak hanya dikenal karena rasanya yang manis, tetapi juga memiliki banyak manfaat bagi kesehatan manusia. Mangga mengandung enzim amilase dan enzim katalase, yang mempunyai peran dalam proses pencernaan dan reaksi biokimia pada tubuh manusia. Tujuan: Penelitian ini bertujuan mengetahui secara detail faktor-faktor yang mempengaruhi aktivasi enzim, dengan fokus pada pengaruh pH, suhu, dan konsentrasi yang berbeda-beda. Metode: Penelitian ini dilakukan di laboratorium dengan menggunakan metode eksperimental, di mana dilakukan pencampuran larutan pada berbagai tingkat pH, yaitu pH 2, pH 7,4, dan pH 9. Selain itu, suhu juga bervariasi dengan pengaturan pada 100 °C, 37 °C, 25 °C, dan 4 °C. Penelitian ini juga melibatkan penggunaan konsentrasi yang berbeda untuk melihat dampaknya terhadap aktivitas enzim. Hasil: Penelitian ini menghasilkan bukti yang jelas bahwa aktivitas enzim sangat dipengaruhi oleh ketiga faktor tersebut, yaitu pH, suhu, dan konsentrasi. Setiap variasi dalam pengaturan kondisi eksperimen menunjukkan perbedaan yang signifikan dalam tingkat aktivitas enzim yang terukur. Kesimpulan: Melalui penelitian ini, diharapkan dapat dibuktikan bahwa aktivitas dari enzim memang bisa terpengaruhi oleh pH, suhu, dan konsentrasi. Setiap uji yang dilakukan menunjukkan kompleksitas interaksi antara faktor-faktor tersebut dalam mempengaruhi kinerja enzim. Penelitian ini dapat memberikan kondisi optimal untuk memaksimalkan aktivitas enzim dalam berbagai aplikasi biokimia.

Comparison of Vitamin C Content in Fresh and Canned Mandarin Oranges (Citrus reticulata Blanco) at a Supermarket in Pontianak Erwansani, Enggy; Reynaldi, Muhammad Andre; Evifani, Dinda Silvia; Fong, Siau; Fendi, Fendi; Stevia, Tessa; Saputra, Rico; Setiawansyah, Arif
Journal of Food and Pharmaceutical Sciences Vol 13, No 3 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.20450

Abstract: Vitamin C is one of the micronutrients needed and cannot be synthesized by the human body so it needs intake from the outside such as fruits. Mandarin oranges are one of the sources of vitamin C that is widely circulated in Pontianak City. This fruit can be available in both fresh and canned form. However, the heating process during the processing of canned fruit can damage the vitamin C content in it. Therefore, an analysis is needed to compare vitamin C levels in fresh and canned mandarin fruits. The methods used were in the form of tube tests using FeCl3, KMnO4, and I2, as well as quantitative tests using uv-vis spectrophotometry. The results showed that the two positive samples contained vitamin C with a level of 78.56912152 mg/100 g in the fresh sample and 31.95655685 mg/100 g in the canned sample. Thus, it can be concluded that the vitamin C level in fresh mandarin oranges is higher than in canned mandarin oranges.

Antibacterial Activity of Crude Ethanolic Leaves Extract of Artocarpus heterophyllus Lam. Cultivated in Toba Region against Staphylococcus aureus Ayu Sibagariang; Muhammad Andre Reynaldi; Rony Sitohang
International Journal of Health Engineering and Technology Vol. 1 No. 5 (2023): IJHET - JANUARY 2023
Publisher : CV. AFDIFAL MAJU BERKAH

Jackfruit leaves (Artocarpus heterophyllus Lam.) was tested for its antibacterial activity against a human pathogenic bacterium, Staphylococcus aureus in vitro. Jackfruit leaves were collected from a forest region in the Toba Regency, North Sumatra and air-dried for five days. The dried leaves were crushed into powder to obtain simplicia and macerated using EtOH. The crude extracts were concentrated in vacuo using a rotary-evaporator and diluted into various concentrations (25, 50, 75, 100%) for antibacterial assay. The results showed that the antibacterial activity of extracts followed a concentration-dependent manner with the highest inhibition as expressed in the diameter of inhibitor zone reached 19 mm. The MIC50 value generated from a linear regression analysis between diameter of inhibition zone and concentration was determined at 10% of extract to display its effective concentration. The findings may be further investigated through phytochemical analysis and the selection of different organic solvents to maximize the bioactivity of jackfruit from this region.

DOCKING SINERGIS ALLICIN, ALLIIN, DAN DIALLYL SULFIDE TERHADAP RESEPTOR ESTROGEN ALFA PADA KANKER PAYUDARA Reynaldi, Muhammad Andre; Faradilla, Aulia; Erwansani, Enggy; Najini, Robby
JIFI (Jurnal Ilmiah Farmasi Imelda) Vol. 9 No. 1 (2025): JIFI (Jurnal Ilmiah Farmasi Imelda)
Publisher : Universitas Imelda Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52943/jifarmasi.v9i1.1982

Integration of Molecular Docking in the Identification of Natural Antioxidants: Interaction Study of Jackfruit Leaf Flavonoids with NADPH:FMN Oxidoreductase Muhammad Andre Reynaldi; Meri Ropiqa
Journal of Food and Pharmaceutical Sciences Article In Press 2026
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.26236

Oxidative stress plays a critical role in the progression of various degenerative diseases through increased production of reactive oxygen species (ROS), driven in spart by the activity of redox related enzymes such as NADPH:FMN oxidoreductase. Bioactive compounds from jackfruit leaves are known to possess antioxidant potential, yet their molecular mechanisms against specific enzymatic targets remain insufficiently elucidated. This study aimed to evaluate the potential interaction of jackfruit leaf flavonoids morin, oxyresveratrol, and artocarpin with NADPH:FMN oxidoreductase using molecular docking analysis. The 1BKJ protein structure was prepared following standard protocols, and all ligands were optimized prior to performing redocking for method validation. AutoDock Vina 1.2.7 was employed with a 20×20×20 Å grid box area. Redocking produced an RMSD of 0.1469 Å, confirming the reliability of the docking parameters. Docking results revealed that morin (–7.848 kcal/mol) and oxyresveratrol (–7.577 kcal/mol) exhibited stronger binding affinities compared with vitamin C (–5.713 kcal/mol) and artocarpin (–5.577 kcal/mol). The dominant interactions involved Arg15, Arg169, Tyr128, Tyr199, and Tyr200, residues that contribute to the stabilization of ligand protein complexes in silico and may be located near functionally relevant regions associated with redox activity. These findings suggest that jackfruit leaf flavonoids may serve as promising candidates for further investigation as potential modulators of redox related enzymes based on predictive in silico evidence.

Integration of Molecular Docking in the Identification of Natural Antioxidants: Interaction Study of Jackfruit Leaf Flavonoids with NADPH:FMN Oxidoreductase Muhammad Andre Reynaldi; Meri Ropiqa
Journal of Food and Pharmaceutical Sciences Article In Press 2026
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.26236

Oxidative stress plays a critical role in the progression of various degenerative diseases through increased production of reactive oxygen species (ROS), driven in spart by the activity of redox related enzymes such as NADPH:FMN oxidoreductase. Bioactive compounds from jackfruit leaves are known to possess antioxidant potential, yet their molecular mechanisms against specific enzymatic targets remain insufficiently elucidated. This study aimed to evaluate the potential interaction of jackfruit leaf flavonoids morin, oxyresveratrol, and artocarpin with NADPH:FMN oxidoreductase using molecular docking analysis. The 1BKJ protein structure was prepared following standard protocols, and all ligands were optimized prior to performing redocking for method validation. AutoDock Vina 1.2.7 was employed with a 20×20×20 Å grid box area. Redocking produced an RMSD of 0.1469 Å, confirming the reliability of the docking parameters. Docking results revealed that morin (–7.848 kcal/mol) and oxyresveratrol (–7.577 kcal/mol) exhibited stronger binding affinities compared with vitamin C (–5.713 kcal/mol) and artocarpin (–5.577 kcal/mol). The dominant interactions involved Arg15, Arg169, Tyr128, Tyr199, and Tyr200, residues that contribute to the stabilization of ligand protein complexes in silico and may be located near functionally relevant regions associated with redox activity. These findings suggest that jackfruit leaf flavonoids may serve as promising candidates for further investigation as potential modulators of redox related enzymes based on predictive in silico evidence.

Integration of Molecular Docking in the Identification of Natural Antioxidants: Interaction Study of Jackfruit Leaf Flavonoids with NADPH:FMN Oxidoreductase Reynaldi, Muhammad Andre; Ropiqa, Meri
Journal of Food and Pharmaceutical Sciences Vol 14, No 1 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.26236

Oxidative stress plays a critical role in the progression of various degenerative diseases through increased production of reactive oxygen species (ROS), driven in spart by the activity of redox related enzymes such as NADPH:FMN oxidoreductase. Bioactive compounds from jackfruit leaves are known to possess antioxidant potential, yet their molecular mechanisms against specific enzymatic targets remain insufficiently elucidated. This study aimed to evaluate the potential interaction of jackfruit leaf flavonoids morin, oxyresveratrol, and artocarpin with NADPH:FMN oxidoreductase using molecular docking analysis. The 1BKJ protein structure was prepared following standard protocols, and all ligands were optimized prior to performing redocking for method validation. AutoDock Vina 1.2.7 was employed with a 20×20×20 Å grid box area. Redocking produced an RMSD of 0.1469 Å, confirming the reliability of the docking parameters. Docking results revealed that morin (–7.848 kcal/mol) and oxyresveratrol (–7.577 kcal/mol) exhibited stronger binding affinities compared with vitamin C (–5.713 kcal/mol) and artocarpin (–5.577 kcal/mol). The dominant interactions involved Arg15, Arg169, Tyr128, Tyr199, and Tyr200, residues that contribute to the stabilization of ligand protein complexes in silico and may be located near functionally relevant regions associated with redox activity. These findings suggest that jackfruit leaf flavonoids may serve as promising candidates for further investigation as potential modulators of redox related enzymes based on predictive in silico evidence.

Integration of Molecular Docking in the Identification of Natural Antioxidants: Interaction Study of Jackfruit Leaf Flavonoids with NADPH:FMN Oxidoreductase Reynaldi, Muhammad Andre; Ropiqa, Meri
Journal of Food and Pharmaceutical Sciences Vol 14, No 1 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.26236

Oxidative stress plays a critical role in the progression of various degenerative diseases through increased production of reactive oxygen species (ROS), driven in spart by the activity of redox related enzymes such as NADPH:FMN oxidoreductase. Bioactive compounds from jackfruit leaves are known to possess antioxidant potential, yet their molecular mechanisms against specific enzymatic targets remain insufficiently elucidated. This study aimed to evaluate the potential interaction of jackfruit leaf flavonoids morin, oxyresveratrol, and artocarpin with NADPH:FMN oxidoreductase using molecular docking analysis. The 1BKJ protein structure was prepared following standard protocols, and all ligands were optimized prior to performing redocking for method validation. AutoDock Vina 1.2.7 was employed with a 20×20×20 Å grid box area. Redocking produced an RMSD of 0.1469 Å, confirming the reliability of the docking parameters. Docking results revealed that morin (–7.848 kcal/mol) and oxyresveratrol (–7.577 kcal/mol) exhibited stronger binding affinities compared with vitamin C (–5.713 kcal/mol) and artocarpin (–5.577 kcal/mol). The dominant interactions involved Arg15, Arg169, Tyr128, Tyr199, and Tyr200, residues that contribute to the stabilization of ligand protein complexes in silico and may be located near functionally relevant regions associated with redox activity. These findings suggest that jackfruit leaf flavonoids may serve as promising candidates for further investigation as potential modulators of redox related enzymes based on predictive in silico evidence.

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