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Fermentasi Etanol Jangka Panjang Khamir IS258 Terimobilisasidalam Beads Matriks Alginat Metode Fed-Batch untuk Pengurangan Penggunaan Kultur Starter Ida Ayu E. P. Wulandari; Indah S. R. Naibaho; Desi N. I. Izzabilah; I M. Mahaputra Wijaya; Ida Bagus W. Gunam; I W. Arnata
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p9

Abstract

Imobilisasi sel menggunakan matrik penjerat (matrix entrapment) merupakan suatu proses untuk menghentikan pergerakan dari sel pada tempat tertentu dalam suatu ruang reaksi dan digunakan sebagai katalis, umumnya digunakan dalam bioteknologi seperti fermentasi etanol karena lebih efisien dan efektif dibandingkan dengan menggunakan kultur bebas. Isolat khamir IS258 adalah isolat unggul yang diambil dari industri arak di Karangasem Bali berfungsi sebagai agen fermentasi bioetanol. Pada penelitian ini, imobilisasi sel khamir IS258 dibuat dengan menggunakan natrium alginat yang berfungsi sebagai bahan penjerat atau pemerangkap (entrapment) sel khamir IS258 untuk membentuk matriks alginat-IS258. Matriks alginat-IS258 yang dibuat kemudian digunakan untuk fermentasi etanol berulang dari analog nira kelapa yang ditujukan untuk mengganti penggunaan starter baru setiap fermentasi baru dilakukan. Pada penelitian ini dilakukan analisis bagaimana kemampuan kondisi fermentasi awal dan kondisi fermentasi berjalan oleh khamir IS258 selama fermentasi analog nira berlangsung. Hasil yang diperoleh bahwa semakin lama khamir IS258 dalam beads matriks alginat digunakan maka ukuran struktur butiran (beads) matriks semakin mengembang. Ini dikarenakan populasi sel khamir IS258 yang meningkat di dalam beads matriks, sehingga ukuran beads membesar dan kemungkinan kekuatan struktur pada alginat semakin melemah. Hasil perhitungan jumlah khamir IS258 menggunakan metode total plate count menunjukkan pada beads baru sebanyak 1,6 x 105 CFU/g, dan beads matriks alginat-IS258 setelah pemakaian berulang selama 140 hari sebanyak 6,7 x 105 CFU/g, sel khamir yang mengendap sebanyak 1,5 x 106 CFU/g, dan cairan hasil fermentasi sebanyak 1,1 x 106 CFU/g. Konsentrasi etanol yang diproduksi semakin lama semakin meningkat konsentrasinya dan imobilisasi IS258 pada kalsium alginat dianggap mampu menggantikan penambahan starter IS258 dan menggantikan metode fermentasi sistem batch.
Analisis Produktivitas Produksi Body Scrub Di PT. XYZ Tinambunan, Nurcahaya; Yoga, I Wayan Gede Sedana; Arnata, I Wayan
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 13 No 4 (2025): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JRMA.2025.v13.i04.p14

Abstract

Productivity is an important indicator in assessing the efficiency and competitiveness of a company. PT XYZ, a SPA product production company established in 2000, experienced an annual decrease in productivity ranging from 1% to 5% during the 2019-2024 period. This decline is influenced by a variety of factors, with the increasing number of defective products being the main causative factor. This study aims to measure the productivity level of body scrub production, identify the causes of low productivity index, and provide recommendations for strategies to increase productivity. The Production, Organization, Sales, Product, Arbeiter, Capital (POSPAC) method is used to measure productivity levels; Fishbone diagrams to analyze the causes of the decline; and the Analytic Hierarchy Process (AHP) to determine the best solution. The results of the analysis show that the elements of production, sales, products, and capital experienced a significant decline during 2020–2024, with the highest decline in 2024 of 5.32%. The main factors that cause this include: high employee absenteeism, raw material inconsistency, broken machinery, less strict QC system, weak safety stock management. The recommended strategies include the implementation of incentive and monitoring systems, preventive maintenance, layered QC, standardization of material composition and moisture level analysis, as well as the formation of a Quality Assurance (QA)team.
Analisis Kualitas Sabun Cair Dengan Bahan Pengikat Dari Filter Puntung Rokok Purba, Ira Sylvia; Arnata, I Wayan; Antara, Nyoman Semadi
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 12 No 4 (2024): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JRMA.2024.v12.i04.p02

Abstract

Waste is an issue of considerable concern in Indonesia. One form of hazardous waste that is difficult to degrade by the environment is cigarette butt filters. Therefore, there is a need for further handling to provide added value to cigarette butt filters by utilizing them as a binder in soap making. This study aims to analyze the quality of soap with binders from cigarette butt filters and determine the weight of liquid soap and soaking time that produces the best soap. This study used two factors: liquid soap weight (35, 40, and 45 g) and soaking time (10, 20, and 30 minutes). The results showed that soaking time and its interaction had a significant effect on foam stability, while the weight of liquid soap did not show a significant effect. In addition, the weight of liquid soap significantly effected moisture content and density, but not pH and antibacterial ability. The effectiveness index shows that the best treatment to produce soap is the ratio of liquid soap weight of 45 g and soaking time of 10 minutes. The overall acceptance test (hedonic) showed that panelists preferred the soap with the ratio W2B2 (40 g liquid soap, 20 minutes soaking), which had a value of 51 ± 133.
Aktivitas Antibakteri Escherichia coli Pada Berbagai Konsentrasi Lignin Dari Serat Serabut Kelapa (Cocos nucifera L) Wahyudiyono, Aditya Ivan; Anggreni, Anak Agung Made Dewi; Arnata, I Wayan
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 12 No 4 (2024): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JRMA.2024.v12.i04.p04

Abstract

Lignin as one of the phenolic compounds can be used as an antibacterial substance that has renewable properties, is easily decomposed by the environment, and its availability is abundant in nature. The purpose of this study is to determine the effect of lignin concentration from coconut fiber on the antibacterial activity of Escherichia coli and determine the concentration of lignin from coconut fiber that produces the highest antibacterial activity of Escherichia coli. This study used a completely randomized design (CRD) with lignin concentration treatment (K) consisting of 6 levels, namely antibiotics, 0 percent, 5 percent, 10 percent, 15 percent, and 20 percent. The variable observed was the diameter of the inhibition zone against E. coli bacteria. The results of the analysis of variance showed that the concentration of lignin had a very significant effect on the diameter of the inhibition zone of E. coli bacteria. Lignin concentration of 20 percent produced the highest inhibition zone diameter of 7,11 mm.
Pengaruh Konsentrasi Minyak Kayu Manis (Cinnamomum Burmanii) Terhadap Daya Hambat Pertumbuhan Jamur Tuti Herlina Br. Naibaho; Nyoman Semadi Antara; I Wayan Arnata
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 11 No 2 (2023): Juni
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JRMA.2023.v11.i02.p11

Abstract

Cinnamon oil (Cinnamomum burmanii) has anti-bacterial and anti-fungal activity because it contains cinnamaldehyde. One of the toxigenic fungi is Saccharomyces cerevisiae which can damage the quality of legen drinks, and Aspergillus niger which causes otomycosis. This study aimed to determine the effect of cinnamon oil concentration as a growth inhibitor for S. cerevisiae and A. niger and determine the best concentration of cinnamon oil to inhibit S. cerevisiae and A. niger fungi.This research is an experimental study using cinnamon oil concentration as a treatment consisting of three concentrations, namely 0.5%, 1%, and 1.5%. Each treatment was tested for its inhibitory activity on two types of fungi, namely Saccahromyces cerevisiae and Aspergillus niger, Each treatment was repeated 2 times. Furthermore, the data obtained is calculated the average value and the standard deviation value, then the data is presented in a graph. The variables observed in this study were the anti-fungal effectiveness of cinnamon oil. Fungal growth inhibition test showed that the concentration cinnamon oil affictedthe growth of Saccharomyces cerevisiae and Aspergillus niger at a concentration of 0.5%; 1%; and 1.5%. Cinnamon oil concentration of 1,5% had a stronger potential to inhibit the growth of Saccharomyces cerevisiae and Aspergillus niger with each power diameters of 10.33 mm and 12.35 mm, and categorized as having strong inhibition activity according to.
Produksi Asinan Cabai Jalapeno (Capsicum Annum) Dengan Variasi Metode Proses Dan Inokulasi Isolat Pr.6.15.2 Lilis Banne Paseru; Nyoman Semadi Antara; I Wayan Arnata
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 11 No 4 (2023): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/

Abstract

Jalapneo peppers contain minerals, fiber, calcium, vitamins and bioactive compounds such as folate, capsaicin, and manganese that are beneficial for health. Pickled is a processed product of vegetables and fruit with the addition of salt, acid or starter culture. This study aims to determine the effect of process method and inoculation of lactic acid bacteria PR. 6.15.2 in the fermentation of jalapeno chili pickles on the best characteristics of the resulting jalapeno chili pickles. The experimental design of this study was a two factorial group randomized design, namely the process method (F) consisting of 2 levels, namely wet fermentation (F1), dry fermentation (F2), Second, namely starter culture inoculation (I) with concentrations of 0%, 2% and 4%. The results showed that the effect of process method and inoculation of PR 6.15.2 isolate and their interaction had a very significant effect on total chlorophyll, total lactic acid bacteria (LAB), total acid, vitamin C content, color, aroma, texture, taste and overall acceptance. Based on overall acceptance, the best treatment combination was wet fermentation with 4% starter culture inoculation with characteristics of total chlorophyll 15.31 mg/g, total LAB 9.41 Log CFU/ml, total acid 4.46%, vitamin C content 2.50%, color 4.00, aroma 4.00, texture 4.01, taste 4.15 (liked).
Pengaruh Konsentrasi Hidrogen Peroksida Dan Waktu Proses Bleaching Terhadap Karakteristik Selulosa Serat Sabut Kelapa (Cocos Nucifera L.) I G. A. A. Utami Andari; I W. Arnata; A. A. M. Dewi Anggreni
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 10 No 3 (2022): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JRMA.2022.v10.i03.p01

Abstract

Indonesia is the largest coconut producing country in the world. The abundant potential of coco fiber has not been utilized optimally. The purpose of this study were to determine the effect of H2O2 concentration and time of the bleaching process on the cellulose’s characteristics from coco fiber, as well as to determine the concentration of H2O2 and time of the bleaching process to produce cellulose from coco fiber with the best characteristics. This study used a factorial Randomized Block Design with two factors, that were concentration of H2O2 (K) (20%, 30% and 40%) and time of the bleaching (W) (60 minutes and 120 minutes). Variables observed were yield, whiteness index, cellulose, hemicellulose and lignin. The concentration of hydrogen peroxide and time of the bleaching process had a very significant effect on all observed variabels. The interaction between treatments had a very significant effect on cellulose, hemicellulose and lignin, a significant effect on the whiteness index, but no significant effect on the yield of coco fiber. The best treatment for the production of cellulose was obtained with a concentration of 40% H2O2 and a bleaching time of 120 minutes. The characteristics of the cellulose produced were 38.45±2.51% yield, 76.23±1.54% whiteness index, 90.19±0.66% cellulose, 5.53±0.58% hem icellulose and 3.66 ±0.18% lignin.
Analisis Faktor-Faktor Bauran Pemasaran Yang Menentukan Keputusan Konsumen Dalam Memilih Minimarket Untuk Berbelanja Di Desa Ungasan I Made Ari K. Jagatkirana; Cokorda Anom B. Sadyasmara; I Wayan Arnata
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 10 No 3 (2022): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JRMA.2022.v10.i03.p05

Abstract

The advantages of minimarkets on marketing mix implementation in Ungasan Village causing the consumers of grocery store switch their shoping choice to minimarkets, that makes grocery store owners should implement a marketing mix strategy to be competitive, by studying the behavior of minimarket consumers. The purpose of this research were to analyze the marketing mix factors that determine consumer decisions in choosing a minimarket to shop in Ungasan Village and to find out the dominant factors, which it can be used as references by grocery stores. Research carried in some stages, started by attribute identification based on marketing mix factors, questionnaire generating, reliability and validity test, deploy the questionnaire to the consumers of grocery store, data gathering, and data analyzing. The sampling method used was purposive method with 154 respondents. The data obtained was then analyzed by using factor analysis. The results showed that there are five marketing mix factors that determine consumer decisions in choosing minimarkets for shopping in Ungasan Village namely accessibility factor, employee performance factor, promotion factor, physical evidence factor, and price factor. The accessibility factor is the dominant factor that determines consumer decisions in choosing a minimarket for shopping, with the attribute that has the highest correlation value is that the distance of the mini market is close to the dwelling.
Pengaruh Jenis dan Konsentrasi Bahan Pemlastis terhadap Bioplastik Glukomanan Aditya Nandika A. J; Bambang Admadi Harsojuwono; I Wayan Arnata
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 9 No 1 (2021): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JRMA.2021.v09.01.p07

Abstract

This research aims to determine the effect of plasticizer types and concentrations on the characteristics of glucomannan bioplastics, and to determine the types and concentrations of plasticizers that can produce glucomannan bioplastics with the best characteristics. This experimental design used a completely randomized design method. Factor I is a type of plasticizer consisting of glycerol, sorbitol, propanol-2, and polyethylene glycol. The second factor is the concentration of plasticizers which consists of 4 levels, namely 0.5%: 1.5%: 2.5%: 3.5%. The experiment resulted in 16 treatment combinations and grouped into 2 groups to obtain 32 experimental units. The data were analyzed for their diversity and continued with the Duncan multiple comparison test. The results showed that the type and concentration of plasticizers had a very significant effect on tensile strength, elongation at break, modulus young, and swelling. The interaction has a very significant effect on tensile strength and expansion and has a significant effect on the elasticity of glucomannan bioplastics. Meanwhile, the type and concentration of plasticizers had no significant effect on the length of biodegradation. The best glucomannan bioplastic was obtained in the treatment of glycerol plasticizers with a concentration of 1.5 % with a tensile strength value of 6.17 MPa, elongation at break of 21.50 %, elasticity 28.72 MPa development 25.84 %, and degradation time of 8 days. Bioplastics produced in this study have meet the SNI 7188.7:2016 standards in the elongation test at break and standards ASTM 5336 in the degradation time variables. The resulting bioplastic has not met SNI on the tensile strength, modulus young, and swelling variables.
Pengaruh Suhu dan Waktu Maserasi terhadap Aktivitas Antioksidan Ekstrak Kulit Buah Kakao (Theobroma cacao L.) Dimas Anggi Ananta; G.P. Ganda Putra; I Wayan Arnata
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 9 No 2 (2021): Juni
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JRMA.2021.v09.02.p04

Abstract

Cocoa pod husk is a by-product of cocoa processing which is quite abundant and has not been used optimally. Cacao pod husk can be used more optimally by extracting, its content of polyphenol compounds which can be used as natural antioxidants. The aim of this study were to determine the effect of temperature and maceration time of cocoa pod husk extract as a source of antioxidants and to determine the best type of temperature and maceration time to produce cocoa pod husk extract as a source of antioxidants. This experiment was designed by using factorial randomized block design. The first factor was type of maceration temperature consisting of 30±2°C, 45±2°C and 60±2°C. The second factor was maceration time, which were done for 24, 36 and 48 h. The data were analyzed with analysis of variance and continued with the Tukey test. The results showed that the temperature and time of maceration had a very significant effect on yield, total phenolic and antioxidant capacity of cocoa pod husk extract. Interactions between treatments had a very significant effect on total phenolic and antioxidant capacity but did not significantly affect the yield of cocoa pod husk extract. The best treatment for producing cocoa pod husk extract as a source of antioxidants was using maceration temperature 60±2°C and maceration time for 36 h with yield characteristics 5,28±0,15%, total phenolic at 168.16±0,06 mg GAE/g and capacity antioxidant 130.94±0.84 mg GAEAC/g.
Co-Authors A. A. M. Dewi Anggreni A.A.S. A. Sukmaningsih Aditya Nandika A. J Aditya Nandika A.J Amirrulloh, Muchammad Rizqi Amna Hartiati Anak Agung Made Dewi Anggreni ANAK AGUNG MADE DEWI ANGGRENI Anak Agung Sagung Inten Mahasari Putri Arnita Adriani Purba Bambang Admadi Bambang Admadi H Bambang Admadi Harjosuwono Bambang Admadi Harsojuwono Bambang Admadi Harsojuwono Bangkit Dwi Satryo Bara, Sari Natalia Batu Cokorda Anom B. Sadyasmara Desi Lusiani Pasaribu Desi N. I. Izzabilah Dewi Sartika Dimas Anggi Ananta Dimas Anggi Ananta Dwi Setyaningsih G. W. Wirayuda G.P. Ganda Putra G.P. Ganda Putra Gede Gora Adrista Gusti Ayu Gayatri Amasdenia Sukarno I G. A. A. Utami Andari I Gusti Agung Ayu Mirah Pradnya Dewi I Gusti Agung Ayu Utami Andari I Gusti Putu Ganda Putra I Kadek Punia Atmaja I Ketut Satriawan I Komang Putra Adnyana I Komang Rai Wisnawa Putra I M. Mahaputra Wijaya I Made Adi Parimartha I Made Ari K. Jagatkirana I Made Ari Kharismananda Jagatkirana I Made Arif Sukmawan I Made Mahaputra Wijaya I Made Mahaputra Wijaya I Made Mahaputra Wijaya i made mateo nautillus I Made Sugiastawa I Made Sugiastawa I Made Topan Wira Aristya I W.G. SEDANA YOGA I Wayan Angga Sukma I Wayan Rai Widarta I. Setyawati Ida Ayu Adi Ida Ayu E. P. Wulandari Ida Ayu Gede Satwika Candra Praba Sari Ida Ayu Sinta Citra Pertiwi Ida Bagus Ananta Wibawa Ida Bagus Wayan Gunam Ida Bagus Wiryasuta Yudiantara Indah S. R. Naibaho Intan Tiar Malau K.G. Govardhana Sutha Karina Novita Dewi Lilis Banne Paseru Lilis Banne Paseru Luh Putu Wrasiati Lutfi Suhendra MADE AYU DHAVA VAGISVARI GIRI PUTRI MADE PHARMAWATI Made Puspa Aridona Manika Santhi Marchela Milenia Rindany Monica Rusiyantoro Monica Rusiyantoro Muhammad Iqbal N.L. Yulianti Ni Made Heni Epriyanti Ni Made Wartini Ni Made Wartini Ni Putu Mega Triasswari Ni Wayan Anik Satria Dewi Ni Wayan Anita Ni Wayan Nadia Martaningsih Sutarsa Ninik Indah Purwati Nur Azizah Diniari Nur Richana NYOMAN SEMADI ANTARA Pranayasa, Wayan Angga Pupung Ferdiansyah Purba, Ira Sylvia Putra, Ferdi Prayoga Rian Hakim Rian Hakim Richard Howard Patty Sadyasmara, Cokorda Anom Bayu Sri Mulyani Sri Mulyani Tinambunan, Nurcahaya Tuti Herlina Br. Naibaho Tuti Herlina Br.Naibaho Wahyudiyono, Aditya Ivan Wijaya, I Made Mahaputra