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All Journal Berkala Ilmiah Pendidikan Biologi (BioEdu) BIOTROPIA - The Southeast Asian Journal of Tropical Biology International Journal of Applied Biology JURNAL PENGABDI Communications in Science and Technology Indonesian Journal of Educational Science (IJES) Sains dan Matematika Jurnal Kependidikan: Jurnal Hasil Penelitian dan Kajian Kepustakaan di Bidang Pendidikan, Pengajaran dan Pembelajaran
Mahanani Tri Asri
Jurusan Biologi Universitas Negeri Surabaya
Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Veterinary Other

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Aktivitas Antibakteri Ekstrak Etanol Lichen Usnea sp. terhadap Pertumbuhan Bakteri Ralstonia solanacearum Silviana, Silviana; Asri, Mahanani Tri
Sains dan Matematika Vol. 7 No. 1 (2022): April, Sains & Matematika
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/sainsmat.v7n1.p20-25

Abstract

Ralstonia solanacearum merupakan bakteri penyebab penyakit layu pada budi daya tanaman hortikultura dan dapat menurunkan hasil produksi hingga 90%.Pengendalian R. solanacearum biasanya dilakukan menggunakan pestisida sintetik, tetapi penggunaan berlebih dan dalam jangka waktu yang panjang dapat menyebabkan bakteri menjadi resisten. Perlu adanya pengendalian penyakit yang ramah lingkungan menggunakan pestisida nabati dengan memanfaatkan senyawa aktif lichen Usnea sp. Penelitian ini bertujuan untuk mendeskripsikan pengaruh ekstrak etanol lumut kerak dengan berbagai konsentrasi dalam menghambat pertumbuhan R. solanacearum dan untuk menentukan konsentrasi optimal ekstrak etanol lumut kerak dalam menghambat pertumbuhan bakteri R. solanacearum secara in vitro. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan variasi konsentrasi ekstrak yang digunakan, yaitu 8%, 6%, 4%, 2%, dan kontrol positif (kloramfenikol 1%), serta kontrol negatif (akuades), masing-masing dengan 4 kali ulangan. Pengujian aktivitas antibakteri dilakukan dengan metode difusi cakram (disk diffusion).Data yang diperoleh berupa diameter zona hambat yang dianalisis dengan ANOVA satu arah dan dilanjutkan uji Duncan. Hasil penelitian menunjukkan bahwa ekstrak lichen Usnea sp. dapat menghambat pertumbuhan bakteri R. solanacearum. Perlakuan dengan konsentrasi 8% merupakan konsentrasi yang optimal dalam menghambat pertumbuhan R. solanacearumyang termasuk dalam kategori sedang, dengan diameter zona hambat sebesar 6,00 ± 0,82 mm.
Lactic Acid Bacteria (LAB) from Fermented Milk Leftover for Wastewater Treatment of Soybean-Based Industries Trimulyono, Guntur; Isnawati; Asri, Mahanani Tri; Khaleyla, Firas
International Journal of Applied Biology Vol. 7 No. 1 (2023): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tempeh and tofu are widely consumed by Indonesians and easily made using simple tools and method, thus numerous home industries producing are emerging. However, soybean processing in these industries produces high volume of wastewater. This study provides a simple wastewater treatment instrument design that could be used by soybean-based home industries by applying a series of physical, chemical, and biological treatment. Physical treatment was performed by filtration, chemical by addition of alum salts, while biological treatment was done by supplementing with lactic acid bacteria (LAB) from leftover container of fermented milk. Wastewater was recorded by its parameters (temperature, pH, dissolved oxygen/DO, biological oxygen demand/BOD, turbidity) at every phase of treatment after incubation for two various of periods; 1 hour and 5 days. Results showed that wastewater treated using instrument design had changes in all of its parameters observed during the course of treatment.  Wastewater treated in all phase of instrument incubated for 1 hour showed parameters approaching wastewater quality standards, thus it was safer to be disposed to environment compared to wastewater incubated for 5 days. Wastewater treatment as designed in the current study can be applied by soybean-based home industries with short incubation time to remediate wastewater produced during soybean processing before disposal.
Pengembangan E-LKPD Interaktif untuk Melatihkan Kemampuan Literasi Digital Peserta Didik Kelas X SMA pada Materi Virus Simamora, Amelia Angelina; Asri, Mahanani Tri
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol. 13 No. 2 (2024)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/bioedu.v13n2.p339-355

Abstract

Abstrak Pandemi mengakibatkan beberapa aspek kehidupan harus beradaptasi dengan pemanfaatan teknologi, salah satunya pada proses pembelajaran. Penggunaan teknologi pada pembelajaran tentunya membutuhkan keterampilan literasi digital yang baik, dengan harapan peserta didik dapat bersikap bijak dalam mengambil dan menanggapi suatu informasi yang didapatkan. Keterampilan literasi digital memiliki kedudukan yang sama pentingnya dengan keterampilan lain, akan tetapi hasil data PISA 2022 dan hasil survei OECD mengatakan bahwa Indonesia memiliki skor relatif rendah dibidang literasi, sehingga dibutuhkan media pembelajaran yang sesuai dengan kemajuan teknologi saat ini, salah satunya yaitu E-LKPD interaktif. Penelitian ini bertujuan untuk menghasilkan E-LKPD interaktif untuk melatihkan kemampuan literasi digital peserta didik pada materi virus yang valid, efektif, dan praktis ditinjau dari empat indikator yang digunakan, yaitu searching, hypertextual navigation, content evaluation dan knowledge assembly dengan menggunakan model 4D. Metode yang digunakan yaitu validasi, tes, observasi dan angket respon. Data dianalisis menggunakan teknik deskriptif kuantitatif dengan hasil validitas sebesar 96,99% (sangat valid), keefektifitasan dari ketuntasan hasil belajar peserta didik sebesar 100% dengan skor N-Gain sebesar 0,5 (kategori sedang) dan angket respon positif sebesar 97,61% (sangat baik), serta hasil kepraktisan dari observasi aktivitas peserta didik dengan persentase sebesar 92,5% (E-LKPD 1) dan 93,3% (E-LKPD 2) dengan kategori keduanya sangat baik. Berdasarkan hasil tersebut, E-LKPD interaktif yang dikembangkan dapat dikatakan valid, efektif dan praktis untuk digunakan dalam pembelajaran. Kata Kunci: E-LKPD interaktif, virus, literasi digital, hasil belajar, keterampilan literasi. Abstract The pandemic has caused several aspects of life to adapt to the use of technology, one of which is the learning process.. The use of technology in learning certainly requires good digital literacy skills, so that students can make informed decision about how to taking and responding the information obtained. Digital literacy skill has the same important position as other skills, but the results of the PISA 2022 data and OECD survey, Indonesia has a relatively low score in the field of literacy. Thus, learning media is needed in accordance with current technological advances, one of which is interactive E-LKPD. This study aims to produce interactive E-LKPD to train students' digital literacy skills on virus material that is valid, effective, and practical in terms of the four indicators used, namely searching, hypertextual navigation, content evaluation and knowledge assembly using the 4D model. The methods used are validation, tests, observation and questionnaire responses. Data were analyzed using quantitative descriptive techniques with validity results of 96.99% (very valid), the effectiviness of the completeness of student learning outcomes of 100% with an N-Gain score of 0.5 (medium category) and positive response questionnaires was 97.61% (very good), as well as the results of practicality from observing student activities with a percentage of 92.5% (E-LKPD 1) and 93.3% (E-LKPD 2) with both categories being very good. Based on the results, interactive E-LKPD developed can be said to be valid, effective and practical for use in learning. Keywords: interactive E-LKPD, virus, digital literacy, learning outcomes, literacy skills.
BIODIVERSITY OF CELLULOLYTIC BACTERIA ISOLATED FROM FERMETODEGE FOR RUMINANTS Isnawati, Isnawati; Lisdiana, Lisa; Asri, Mahanani Tri; Trimulyono, Guntur
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.1904

Abstract

ARTICLE HIGLIGHTS- Fermetodege as a source of cellulolytic bacteria that degrade cellulosic materials.- Enhances ruminant feed by improving palatability and digestibility.- Utilizes water hyacinth, reducing invasive plant spread and supporting ecosystems.- Bioprospecting reveals new bacteria for effective fermentation starter development.ABSTRACTFermetodege is a name of fermented ruminant feed produced from free heavy metals water hyacinth, rice bran, and corn cobs. Its quality, as well as the fermentation process, are affected by numerous factors, such as the diversity, evenness, and dominance of indigenous cellulolytic bacterial species. A proper understanding of these influential factors is needed to improve the quality of the fermented feed. Therefore, this study aims to evaluate the diversity, evenness, and dominance of cellulolytic bacteria isolated from fermetodege. The cellulolytic activity of the isolates was tested by observing their growth on the carboxymethylcellulose (CMC) media. Isolates with cellulolytic activity formed a clear zone after the colonies were soaked with 0.1% Congo red and rinsed with 1 M NaCl. The Shannon-Weiner's diversity and evenness indexes, as well as Simpson's species dominance, were then calculated. The result showed that the Shannon-Weiner’s diversity index ranged from 0.6849 to 1.8173, and it was categorized as medium. The evenness index was between 0.7778 and 0.9983, which indicates a stable distribution of species. Meanwhile, the Simpson’s species dominance ranged from 0.1835 to 0.5082, which implies that none of the species was dominant. These results show that fermetodege is potentially a source of bacterial isolates and can be used as a fermentation consortium starter.
Creating an E-Module on Environmental Pollution Based on Tegal Deso–Okol Local Wisdom: Can It Enhance Students’ Creative Thinking Skills? Firnanda, Diah Dwi; Asri, Mahanani Tri; Faizah, Ulfi; Rakhmawati, Ismi
Jurnal Kependidikan : Jurnal Hasil Penelitian dan Kajian Kepustakaan di Bidang Pendidikan, Pengajaran, dan Pembelajaran Vol. 11 No. 4 (2025): December (IN PROGRESS)
Publisher : LPPM Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jk.v11i4.18177

Abstract

This study aims to develop a valid, practical, and effective learning e-module based on the local wisdom of Gresik (Tegal Deso–Okol) for environmental pollution content to enhance students’ creative thinking skills. The research employed a Research and Development (R&D) method integrating the ADDIE model (Analysis, Design, Development, Implementation, Evaluation) with a project-based learning (PjBL) framework. Sixty students from a senior high school in Gresik tested the e-module. Evaluation instruments included validation sheets, student questionnaires, performance rubrics, and learning outcome tests. The validation sheets assessed the feasibility of the e-module, the questionnaires measured practicality, and the performance rubrics evaluated project implementation and presentation. Data were analyzed using descriptive statistics, supported by qualitative feedback to refine the final product. The e-module demonstrated high reliability (92.70) and strong validity (3.77). Its practicality reached 91.67% in Class A and 95.83% in Class B. Students’ creative thinking skills improved substantially, with mean scores increasing from 43.23 to 86.97 in Class A and from 50.47 to 90.01 in Class B. The student response rate (97.5%) and the N-gain score (0.80) also indicate high effectiveness. Overall, integrating cultural values into learning activities through this local-wisdom-based e-module effectively supports the development of students’ creative thinking skills.
Co-Authors Afifah, Yeni Nur Ahmad Bashri Al Hafidl, Achmad Naufal Aprillia, Isna Nadia Ashabul Kahfi, Ashabul Dzulkarnain, Shod Abdurrachman Evie Ratnasari Firnanda, Diah Dwi Guntur Trimulyono ISNAWATI Isnawati Isnawati Isyrak, Luthfiyah Judianti, Oki W. D. Khaleyla, Firas Lisa Lisdiana Maria Monica Sianita Nabila, Mutiara Azfa Ningtyas, Listya Eka Nita Kusumawati Nur Ducha Purnama, Erlix Rakhma Rahmawati, Khofifatul Rakhmawati, Ismi Ramadhani, Astri Retno Dwi Setiawan, Ferdiansyah Silviana Silviana Simamora, Amelia Angelina Tianah, Mega Setiyalis Ulfi Faizah