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All Journal AL KAUNIYAH Teknosains: Media Informasi Sains dan Teknologi Prosiding Seminar Biologi BIOTROPIC The Journal of Tropical Biology Biota Journal of Islam and Science TROPICAL GENETICS Filogeni: Jurnal Mahasiswa Biologi Tropical Genetics
Isna Rasdianah Aziz
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Social Sciences

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Journal : Tropical Genetics

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Extraction of DNA on human bone powder Muhammad Edhil Akbar Bur; Muhammad Tasri Hidayat; Isna Rasdianah Aziz; Setia Betaria Aritonang
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Bone is a dynamic network that has a complex cellular regeneration system. A forensic examination is closely related to examining the physiology and anatomy of the living body, including bone as the evidence in DNA testing. This study aims to analyze the DNA extraction on human bone powder using PrepFiler® BTA Forensic DNA Extraction Kit. DNA was isolated from compact and cancellous bone tissue from decomposed human bodies. The bones were soaked in NaOCl for 5 min, then rinsed with nuclease-free water. The bone was sawn into bone powder and then extracted using 220 µl of BTA lysis, 7 µl of Prot-K, and 3 µl of DTT. PrepfilerTM Lysis Buffer was added as much as 300 µl then homogenized by vortex and spin using a centrifuge. Spectrophotometry was performed to measure the DNA concentration using an absorbance from 230 nm to 320 nm. The results showed that the DNA purity values of the three samples of compact and cancellous bone powder used were close to good quality: 2.08, 2.06, and 1.71, respectively. Low concentration values obtained from compact bone samples were 14.2 ng/µL and 11.9 ng/µL respectively, which inversely proportional to cancellous bones by 59 ng/µL.
First record of characterization C-KIT mutation in buccal swab DNA of piebaldism suspect balo from balo community, Pujananting South Sulawesi Muthiadin, Cut; Arisuryanti, Tuty; Aziz, Isna Rasdianah; Hajrah, Hajrah; Khatimah, Khusnul; Hidayat, Muhammad Tasri
Tropical Genetics Vol. 4 No. 1 (2024): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v4i1.67

Abstract

ABSTRACT Piebaldism is a disorder in the area of ​​the body caused by an autosomal genetic predominance of the development of melanocytes.The method used in this study is a qualitative method which aims to determine the variation of the KIT-c gene present in the To Balo mucosa samples.The results of this study indicate thatThe change is caused by a point mutation process where in patients with piabeldism the nucleotide bases of sequence 1391 and 1392 undergo changes, where the normal nucleotide base adenine (A) changes to cytosine (C) and cytosine (C) changes to adenine (A). So it can be concluded that the To Balo community in Bulo-Bulo village, Pujananting District, Barru Regency, South Sulawesi suffers from an autosomal dominant hereditary disease or piebaldism.
Co-Authors Alfian Alfian Alifah Zul Amnah Alir, Rahmat Fajrin Amnah, Alifah Zul Arwan Sugiharto Auriga, Fitriani Baiq Farhatul Wahidah Bestari, Zamra Burhan, Eliazar Cut Muthiadin Fanny Dhea Suryafly FATIMA, SITI Firdayana Firdayana Hafsan, Hafsan Hajrah Hajrah Hajrah, Hajrah Hermawan, Isna Ainun Hidayat, Muhammad Tasri Ipha Ridhayanie Ismail Ismail Khatimah, Khusnul Mahfut Masse, Irmawati Mayangsari, Tirta Miftahuljanna, Miftahuljanna Muh. Khalifah Mustami Muhammad Edhil Akbar Bur Muhammad Tasri Hidayat Nur Adinda Irtiyah Jaenuddin Nurfaizah Nurfaizah, Nurfaizah Nurlailah Mappanganro Rahmani, Tara Puri Ducha Rais, Evi Erviani Safiah, Andi Sitti Setia Betaria Aritonang Sri Musdalifa Suharpina Suharpina Surdianah, Sitti Suryafly, Fanny Dhea Tuty Arisuryanti, Tuty Upreti, Brij Mohan