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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Communications in Science and Technology Jurnal Ilmu Kefarmasian Indonesia ASEAN Journal of Chemical Engineering International Journal of Social Service and Research Makara Journal of Technology Journal of Life Science and Technology
SAHLAN, MUHAMAD
Department Of Chemical Engineering, Faculty Of Engineering, Universitas Indonesia, Kampus UI Depok 16424, Indonesia. Research Center For Biomedical Engineering, Faculty Of Engineering, Universitas Indonesia, Kampus UI Depok 16424, Indonesia
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Journal : Makara Journal of Technology

 1 
Expression and the Functional Study of Fusion Proteins α-Amylase and Hemolysin- αas an Application in Biofilm Polysaccharide Degradation Sugiarta, Gede Yuda; Wiseso, Anggoro; Sari, Siska Yuliana; Kamila, Etri Dian; Geraldine, Vanessa; Christina, Diana; Hanifi, Muhammad; Satyapertiwi, Dwiantari; Hertanto, Robby; Bela, Budiman; Yohda, Masafumi; Sahlan, Muhamad
Makara Journal of Technology Vol. 20, No. 3
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Biofilm is an aggregate of consortium bacteria that adhere to each other on a surface. It is usually protected by the exopolysaccharide layer. Various invasive medical procedures, such as catheterization, endotracheal tube installation, and contact lens utilization, are vulnerable to biofilm infection. The National Institute of Health (NIH) estimates 65% of all microbial infections are caused by biofilm. Periplasmic α-amylase (MalS) is an enzyme that hydrolyzes α-1, 4- glicosidic bond in glycogen, starch, and others related polysaccharides in periplasmic space. Another protein called hemolysin-α (HlyA) is a secretion signal protein on C terminal of particular peptide in gram negative bacteria. We proposed a novel recombinant plasmid expressing α-amylase and hemolysin-α fusion in pSB1C3 which is cloned into E.coli to enable α-amylase excretion to extracellular for degrading biofilm polysaccharides content, as in starch agar. Microtiter assay was performed to analyze the reduction percentage of biofilm by adding recombinant E.coli into media. This system is more effective in degrading biofilm from gram positive bacteria i.e.: Bacillus substilis (30.21%) and Staphylococcus aureus (24.20%), and less effective degrading biofilm of gram negative i.e.: Vibrio cholera (5.30%), Pseudomonas aeruginosa (8.50%), Klebsiella pneumonia (6.75%) and E. coli (-0.6%). Gram positive bacteria have a thick layer of peptidoglycan, causing the enzyme to work more effectively in degrading polysaccharides.
Encapsulation Process of Propolis Extract by Casein Micelle Improves Sunscreen Activity Sahlan, Muhamad; Dienayati, Dara; Hamdi, Darul; Zahra, Soraya; Hermansyah, Heri; Chulasiri, Malyn
Makara Journal of Technology Vol. 21, No. 1
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Propolis, a wax bee glue, have antioxidant activity and effective as broad spectrum UVB and UVA photoprotection sunscreens agent. To improve photoprotection activity in sunscreen cream, the propolis was encapsulated by casein micelle. The Indonesian propolis was extracted by ethanol and separated the wax by freeze precipitation. The extract was encapsulated by casein and reduced their size by ball mill homogenizer with the encapsulation efficiency about 80% and size of particles about 80 nm. The particles was applied in sunscreen cream, evaluate their stabillity and photoprotection activity Sun Protection Factor (SPF) spectrophotometer (Optometric 290s). The results shows that the selected creams were stable and have moderate photoprotection activities higher than the controls. The encapsulation process of propolis by casein micelle can improve their photoprotection activity.
Isolation and Molecular Weight Characterization of Tetragonula laeviceps Honey Protein Sahlan, Muhamad; Azizah, Nurul; Hakamada, Kazuaki; Noguchi, Keiichi; Yohda, Masafumi
Makara Journal of Technology Vol. 22, No. 1
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The concentration of protein in honey is lower than polysaccharide. However, recently the honey’s protein is intensively studied and it showed that protein also have several biological activities such as antibacterial activity. The purpose of this research is to isolate and characterize Tetragonula laeviceps honey protein by determining the molecular weight. Honey protein of Trigona laeviceps was isolated using ultra-filtration with the membrane’s size of 30 kDa, then concentrated using tube membrane size 10 kDa. Molecular weight was analyzed by SDS PAGE. From the analysis, there was major protein band in honey produced by Tetragona laeviceps identified. The produced molecular weight of major protein bands were about 87 and 65 kDa. Determining of the molecular weight of this protein could be used to detect the originality of Tetragonula laeviceps honey from Indonesia.
Determination of the Kinetic Parameters of Cholesterol Oxidation using Cholesterol Oxidase from Streptomyces sp. Perdani, Meka Saima; Hermansyah, Heri; Sahlan, Muhamad; Putri, Dwini Normayulisa; Pambudi, Teguh; Hasibuan, Anggi Khairina Hanum
Makara Journal of Technology Vol. 28, No. 3
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Cholesterol oxidase (CO) was successfully produced from Streptomyces sp. via the submerged fermentation method, and 69 U/mL enzyme activity was obtained. This study aimed to determine cholesterol oxidation kinetics and the production of CO as a catalyst. The enzyme was diluted to 0.15, 0.075, and 0.00375 U/mL for the oxidation reaction. The substrate was also prepared in three concentrations: 3.23, 6.46, and 12.93 mM. The optimization of conditions for enzymatic cholesterol oxidation was investigated through measurement of the effect of initial cholesterol and enzyme concentrations. Cholesterol concentration was rapidly measured via high-performance liquid chromatography (HPLC). The kinetics of CO were modeled using the first-order irreversible reaction. An enzymatic kinetic model was derived, and it was verified using experimental data and sensitivity analysis. Based on the experiment, the highest enzyme concentrations of crude and commercial CO can oxidize the substrate up to 84% within 240 min. However, the oxidation reaction showed a slightly different behavior in the early 60 min, and crude CO exhibited a slower substrate oxidation. The kinetic rate constant obtained by Euler’s method reached 1.0 x 10−3/min and 1.41 x 10−3/min for 0.15 U/mL crude and commercial CO, respectively.
Co-Authors Abdul Mun’im Adra Amalia Nur Ahlina Alya Fadhila Ismail AMARILA MALIK ANGGI KHAIRINA HANUM HASIBUAN Anondho Wijanarko Araz Meilin Asep Bayu Budiman Bela Christina, Diana Chulasiri, Malyn Deti Purwanti Diah Kartika Pratami Diana, Akso Dienayati, Dara Dwi Anggar Yusika Dwiranti, Astari Geraldine, Vanessa Hakamada, Kazuaki Hamdi, Darul Hanif Yuliani Hanifi, Muhammad Heri Hermansyah Hertanto, Robby Hiroshi Hashimoto Istikomah, Iis izzul islam Kamila, Etri Dian Kaysa Faradis Mahira Khayrani, Apriliana Cahya Kuntadi Kuntadi, Kuntadi Lischer, Kenny MUHAMMAD IQBAL Nadhifa Tazkia Ramadhani Nidaan Khafian Noguchi, Keiichi NUNUK WIDHYASTUTI Nur Elistiani Eksadita NURUL AZIZAH Perdani, Meka Saima Pujianto, Paksi Purwanti, Lucia Purwanto, Didik B. Putri, Dwini Normayulisa Putrika, Afiatry Ratna Yuniati RIKA RAFFIUDIN Ryo Miyata Sari Honda Sari, Siska Yuliana Satyapertiwi, Dwiantari Shigenori Kumazawa SISWA SETYAHADI Siti Farida Sri Rahayu, Amy Yayuk Sugiarta, Gede Yuda Teguh Pambudi Teti Indrawati TRI BASTUTI PURWANTINI Wiseso, Anggoro Yelin Adalina Yohda, Masafumi Yoshinobu Ishikawa YUKI DESIANDINI Zahra, Soraya