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UJI AKTIVITAS ANTI BAKTERI EKSTRAK ETANOLIK UMBI AKAR BATU (Coccinia grandis L.Voight) TERHADAP BAKTERI SALMONELLA SP Rukmana, Rizal Maarif; Nugroho, Rahmat Budi; Wisnumurti, Dwi Admani
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8390

AbstractSalmonella sp are bacteria that often infect human and animal. This type of bacteria can cause Salmonellosis in human. Previous research has been carried out on secondary metabolites of plants as medicinal ingredients. One of the plants that has potential as a medicine is stone Akar Batu (Coccinia grandis L Voight). This study aims at examining the antibacterial activities of ethanolic extract of stone Akar Batu on Salmonella sp. The extraction method was carried out by maceration and the solvent used was ethanol 96%. Antibacterial activity was conducted using diffusion method. Identification of compound groups of extracts was done by reacting extracts with various chemical reagents. The results showed that stone of Akar Batu powder had a moisture content of 5.99% and a yield of 1.76%. The identification results of ethanolic extract of stone Akar Batu chemical group indicated that the extract contained flavonoids, alkaloids, saponins and tannins. The results of antibacterial activity test have revealed that ethanolic extract of stone Akar Batu can inhibit Salmonella sp.

Exploring the Anti-Photoaging Potential of Bacillus cereus Superoxide Dismutase in New Zealand Rabbit Skin Indrayati, Ana; Nurfitriyawatie; Rukmana, Rizal Maarif
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.357-366

Bacillus cereus is a heat-resistant bacteria that produces the enzyme superoxide dismutase (SOD). SOD is a metalloenzyme that can eliminate free radicals as the primary cause of photoaging. The point of this study is to find out how well B. cereus works as an anti-photoaging agent on the skin of rabbits’ backs. The SOD enzyme was isolated from B. cereus using the colorimetric method and characterized for its activity. It was encapsulated by the liposome method, while the activity was measured on the rabbit’s back skin exposed to ultraviolet-A (UV-A) radiation with the parameters of moisture, elasticity, and collagen levels using a skin analyzer. At the end of the treatment, a histological test for the animal skin was performed. The particle size analysis (PSA) results on the SOD in liposomes showed nanoparticles with an adsorption efficiency of 85.0% and a zeta potential of 28.0±11.7 mV. SOD encapsulated in the liposomes also indicated anti-photoaging activity potential by increasing collagen, elasticity, and moisture levels. In addition, histological tests revealed that the collagen density in the SOD liposome treatment was higher than the negatively of control and normally of control but lower than the liposome collagen.

ANTIMALARIAL MEDICINAL PLANTS USED BY TRADITIONAL HEALERS IN BENGKULU PROVINCE OF INDONESIA Susanti, Dian; Putra, Aditya Dwi Permana; Safrina, Devi; Wijaya, Nur Rahmawati; Adi, M Bakti Samsu; Mujahid, Rohmat; Rukmana, Rizal Maarif; Subositi, Dyah; Haryanti, Sari; Siswanto, Usman; Widiyastuti, Yuli
BIOTROPIA Vol. 31 No. 3 (2024): BIOTROPIA Vol. 31 No. 3 December 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.3.2318

Article Highlights- This research provides information on the use of medicinal plants for treating malaria among ethnic groups in Bengkulu Province.- This research was part of RISTOJA (Research on Medicinal Plants), national research conducted by the Ministry of Health of the Republic of Indonesia.- Several compounds found in medicinal plants used by traditional healers in the Bengkulu Province have been scientifically proven to be effective as antimalarial medication.AbstractMalaria is a disease with a high mortality rate and is still a significant problem in Indonesia and globally. One of the provinces in Indonesia that uses a diversity of medicinal plants to treat malaria is Bengkulu Province. This research aims to inventory and evaluate the use of medicinal plants as malaria drugs in Bengkulu Province. The research methods used were observation, interviews, and the collection of samples. Respondents were traditional healers from seven ethnic groups in Bengkulu. Plant species, use value (UV) parameters, fidelity level (FL), family use value (FUV), relative frequency of citation (RFC), and plant parts value (PPV) were analyzed. This study found 32 specific herbal concoctions and identified the use of 47 plant species in 28 families used by 20 healers in 6 ethnic groups of Bengkulu. The species Carica papaya, Peronema canescens, and Tinospora crispa have the highest use value, fidelity level, and relative frequency of citation. The Lamiaceae and Caricaceae families have the highest family use value (0.15). The leaves (43.9%) and barks (13.6%) were the most frequently utilized parts of medicinal plants to overcome malaria. Decoction was the method most widely used by traditional healers in Bengkulu to prepare conventional medicine formula.

Phytochemical Profile, Antioxidant Activity and Anticancer Activity of Gamma-Irradiated Black Rice Bran (Oryza sativa L.) Ethanolic Extract: In-Vitro and In-Silico Study Rukmana, Rizal Maarif; Silfarohana, Rantika; Putra, Aditya Dwi Permana; Devi Safrina; Dian Susanti; Nur Rahmawati Wijaya; M Bakti Samsu Adi; Muhammad Evy Prastiyanto; Yanuar Ashari Cahyaningrum; Rina Nurmaulawati; Weka Sidha Bhagawan
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.628-643

Black rice is a food crop with black pigments, which helps reduce the risk of various diseases and improve health. One way to improve food quality and avoid food contaminants is by irradiating. This study evaluated the phytochemical profile, antioxidant activity, and anticancer activity of irradiated black rice bran extract (IBRBE) in vitro and in silico. Black rice was irradiated with a Gamma cell 220 type irradiator at a 7.5 kGy/hour dose rate. Extraction of irradiated black rice bran was carried out using the maceration method. Phenolic and flavonoid components were quantified using the Folin–Ciocalteau and AlCl3 methods, respectively. Phytochemical compounds were identified by liquid chromatography-high-resolution mass spectrometry (LC HRMS). The antioxidant activity of IBRBE was carried out against 2,2-diphenyl-1picrylhydazyl (DPPH). The cytotoxic activity of IBRBE against WiDr cells (colorectal cancer) and Vero (nonhuman cell lines) used the MT test method. Prediction of the inhibitory mechanism of compounds in the extract against target proteins EGFR and GPX7 was carried out in silico. Total phenolics and flavonoids were 2.57 ± 0.28 mg GAE/g and 19.12 ± 0.18 μg QE/ml, respectively. Twenty-four types of active compounds were obtained in IBRBE. The results of antioxidant activity obtained an IC50 value of 1198.45 ± 92.86 μg/ml. IC50 in WiDr cells and Vero cells were obtained at 36.08 ± 11.71 μg/ml and 570.58 ± 130.25 μg/ml, respectively. In silico results, the compound 4 Dodecylbenzene sulfonic acid has the highest binding affinity to the EGFR protein, with a value of -5.9. Meanwhile, the Monoolein compound has the highest binding affinity to the GPX7 protein, with a value of -5.4.

Analysis of Non Specific Parameters of Simplicia Bloodleaf Plant (Iresine herbstii Hook) During Storage Putra, Aditya Dwi Permana; Safrina, Devi; Susanti, Dian; Adi, M. Bakti Samsu; Wijaya, Nur Rahmawati; Rukmana, Rizal Maarif
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2024: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Bloodleaf plant, scientifically known as Iresine herbstii Hook, is an ornamental shrub with possible medicinal properties. Storage of simplicia is necessary to guarantee the accessibility of raw materials from I. herbstii. Prolonged storage of simplicia can impact its quality, including many non-specific factors. Indeterminate parameters suggest a relationship between the characteristics of Hook's I. herbstii simplicia during extended storage. This study seeks to ascertain the impact and correlation between the duration of storage of simplicia and the non-specific characteristics of I. herbstii simplicia. Moisture content, extractive value, and ash content were examined in samples with shelf lives of 0 month (IH0), 4 months (IH4), 10 months (IH10), 20 months (IH20), and 32 months (IH32). The acquired data were analyzed using DMRT (Duncan's Multiple Range Test) and MFA (Multiple Factor Analysis). The analysis results indicated that the storage duration impacted all parameters examined except acid-insoluble ash concentration. Prolonged storage of I. herbstii simplicia decreased the concentration of water-soluble and alcohol-soluble extractive value. At the same time, the water content and total ash content increased. The MFA analysis revealed a strong link between non-specific parameters and the duration of storage of I. herbstii simplicia, except for acid-insoluble ash concentration.

UJI AKTIVITAS ANTI BAKTERI EKSTRAK ETANOLIK UMBI AKAR BATU (Coccinia grandis L.Voight) TERHADAP BAKTERI SALMONELLA SP Rukmana, Rizal Maarif; Nugroho, Rahmat Budi; Wisnumurti, Dwi Admani
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8390

AbstractSalmonella sp are bacteria that often infect human and animal. This type of bacteria can cause Salmonellosis in human. Previous research has been carried out on secondary metabolites of plants as medicinal ingredients. One of the plants that has potential as a medicine is stone Akar Batu (Coccinia grandis L Voight). This study aims at examining the antibacterial activities of ethanolic extract of stone Akar Batu on Salmonella sp. The extraction method was carried out by maceration and the solvent used was ethanol 96%. Antibacterial activity was conducted using diffusion method. Identification of compound groups of extracts was done by reacting extracts with various chemical reagents. The results showed that stone of Akar Batu powder had a moisture content of 5.99% and a yield of 1.76%. The identification results of ethanolic extract of stone Akar Batu chemical group indicated that the extract contained flavonoids, alkaloids, saponins and tannins. The results of antibacterial activity test have revealed that ethanolic extract of stone Akar Batu can inhibit Salmonella sp.

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