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Journal : Nusantara Medical Science Journal

DETECTION OF NONTUBERCULOUS MYCOBACTERIA (NTM) IN ISOLATES FROM SUSPECTED TUBERCULOSIS USING PCR IS6110 GENE Andi Nur Asrinawaty; Muh Nasrum Massi; Rizalinda Sjahril
Nusantara Medical Science Journal Volume I No. 1 Januari-Maret 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v0i1.2203

Abstract

ABSTRACT  Introduction: NTM is a group of mycobacterium other than M.tuberculosis complex and M.Leprae, which found in the environment and can be pathogenic. Objectives: This study aim was to detect NTM isolates using PCR on target genes IS 6110. Methods: This study was conducted in Laboratory of Microbiology, Faculty of Medicine, Hasanuddin University and Laboratorium microbiology in Hasanuddin University Hospital. 14 sample isolates of NTM from sputum of suspected tuberculosis patients. The method using PCR IS6110 gene. Results: The results showed that of 14 samples, 8 positive samples with M. tuberculosis and 6 samples negative. Conclusions: IS6110 PCR can be used to detect NTM isolates from sputum of suspected tuberculosis patients. Keywords : NTM, PCR, IS6110, M.tuberculosis
The Turbidity, pH, Direct Gram as Predictors of Symptomatic Urinary Tract Infections in Pregnant Women Dhian Karina Aprilani Hattah; Rizalinda Sjahril; Sriwijaya Sriwijaya
Nusantara Medical Science Journal Volume 7 Issue 2, July - December 2022
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v7i2.20028

Abstract

Introduction: Urinary tract infections (UTIs) are common in the community, including among pregnant women. This study investigates turbidity, urine pH, and direct gram as predictors of symptomatic urinary tract infection in pregnant women. Methods:  A total of 177 pregnant women with suspected UTIs based on clinical and laboratories at the community health center in Makassar were collected from September to December 2021. The Urine sample was assessed for turbidity, urine pH, direct gram, and urine culture. Results: Urine turbidity was found in 108 (75.5%) patients who had positive cultures and PPV of 94.7%. The mean pH of the urine from UTI patients was 6.42 and without UTIs was 6.45. Urine that grows Gram-positive bacteria showed the most alkaline pH (mean pH=6.71) and was significantly less acidic than urine with UTI negative. In contrast, urine with Gram-negative bacteria had the most acidic pH (mean PH=6.30). The gram direct test shows a significant association with UTIs.  Conclusions: Turbid urine can predict urinary tract infections in pregnant women that provide clinical symptoms. In addition, leukocytes esterase test further selected the samples. Urine pH showed no significant correlation with UTI. Less acidic urine pH is particularly associated with Gram-positive bacterial infections. Furthermore, direct gram can predict the event of UTI.