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All Journal Nusantara Medical Science Journal Alami Journal (Alauddin Islamic Medical) Journal Jurnal Biomedika Jurnal Midwifery JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan)
Rizalinda Sjahril
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Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Prevalence of Listeria spp. with PRS Genes in Fresh Vegetables from Traditional Markets A. Kaisar Adiwijaya Putra; Rizalinda Sjahril; Arif Santoso; Dianawaty Amiruddin; Andi Alfian Zainuddin; Firdaus Hamid
Biomedika Vol 13 No 2 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i2.842

Abstract

Listeriosis is a foodborne infectious disease caused by Listeria monocytogenes and is considered a serious health problem, due to the severity of symptoms and a high mortality rate in worldwide This study aims to identify and determine the prevalence of Listeria species through prs gene screening of fresh vegetables distributed in several markets of Makassar City. A total of 57 fresh vegetable samples were collected from February to May 2019 in four traditional markets Makassar. The isolates is examines to phenotypically and genotypically Vitek and Multiplex PCR with prs and lmo1030 primer. Phenotype analysis did not show the presence of Listeria species, but the results of genotypic was found 8 positive prs gene samples (14.03%), consisting of 31.2% long beans, 18.2% cabbage, and 9.1% cucumber and. All Listeria species found in this study is Listeria monocytogenes. This study also provide information and additional data that prs genes can be used as screening genotype for identify Listeria species in fresh vegetables.
DETECTION OF NONTUBERCULOUS MYCOBACTERIA (NTM) IN ISOLATES FROM SUSPECTED TUBERCULOSIS USING PCR IS6110 GENE Andi Nur Asrinawaty; Muh Nasrum Massi; Rizalinda Sjahril
Nusantara Medical Science Journal Volume I No. 1 Januari-Maret 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v0i1.2203

Abstract

ABSTRACT  Introduction: NTM is a group of mycobacterium other than M.tuberculosis complex and M.Leprae, which found in the environment and can be pathogenic. Objectives: This study aim was to detect NTM isolates using PCR on target genes IS 6110. Methods: This study was conducted in Laboratory of Microbiology, Faculty of Medicine, Hasanuddin University and Laboratorium microbiology in Hasanuddin University Hospital. 14 sample isolates of NTM from sputum of suspected tuberculosis patients. The method using PCR IS6110 gene. Results: The results showed that of 14 samples, 8 positive samples with M. tuberculosis and 6 samples negative. Conclusions: IS6110 PCR can be used to detect NTM isolates from sputum of suspected tuberculosis patients. Keywords : NTM, PCR, IS6110, M.tuberculosis
Isolasi Antimikroba Baru dari Bakteri Tanah Evi Damayanti; Firdaus Hamid; Rizalinda Sjahril
JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) Vol 7, No 2 (2022)
Publisher : Prodi Kesehatan Masyarakat Fakultas Kesehatan Masyarakat UIN Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.444 KB) | DOI: 10.30829/jumantik.v7i2.11266

Abstract

Antimicrobial resistance has become a problem and a threat to health both in Indonesia and in the world. Resistance is the ability of bacteria to neutralize and weaken the action of antibiotics. The aim of this study was to isolate a novel antimicrobial from soil bacteria. The method used in this research is exploratory. The test bacteria used were Staphylococcus aureus and Pseudomonas aeruginosa. The results showed that there were two isolates that had zones of inhibition against the test bacteria staphylococcus aureus, namely isolates T2.2 and T2.18. The results of the first antimicrobial test for isolate T2.2 showed that the inhibition zone formed was 14.05 mm and isolates T2.18 the inhibition zone formed was 11.96 mm. The results of the second antimicrobial test were carried out by treating the unheated T2.2 isolate which showed an inhibition zone formed of 15.53 mm and the heated one 15.46 mm, while the unheated T2.18 isolate showed an average inhibition zone of 12.46 mm and heated 12.21 mm. The conclusion of this study is that the inhibition zone formed on isolates T2.2 and T2.18 is still relatively small and both isolates are spesies of Pseudomonas aeruginosa.
The Turbidity, pH, Direct Gram as Predictors of Symptomatic Urinary Tract Infections in Pregnant Women Dhian Karina Aprilani Hattah; Rizalinda Sjahril; Sriwijaya Sriwijaya
Nusantara Medical Science Journal Volume 7 Issue 2, July - December 2022
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v7i2.20028

Abstract

Introduction: Urinary tract infections (UTIs) are common in the community, including among pregnant women. This study investigates turbidity, urine pH, and direct gram as predictors of symptomatic urinary tract infection in pregnant women. Methods:  A total of 177 pregnant women with suspected UTIs based on clinical and laboratories at the community health center in Makassar were collected from September to December 2021. The Urine sample was assessed for turbidity, urine pH, direct gram, and urine culture. Results: Urine turbidity was found in 108 (75.5%) patients who had positive cultures and PPV of 94.7%. The mean pH of the urine from UTI patients was 6.42 and without UTIs was 6.45. Urine that grows Gram-positive bacteria showed the most alkaline pH (mean pH=6.71) and was significantly less acidic than urine with UTI negative. In contrast, urine with Gram-negative bacteria had the most acidic pH (mean PH=6.30). The gram direct test shows a significant association with UTIs.  Conclusions: Turbid urine can predict urinary tract infections in pregnant women that provide clinical symptoms. In addition, leukocytes esterase test further selected the samples. Urine pH showed no significant correlation with UTI. Less acidic urine pH is particularly associated with Gram-positive bacterial infections. Furthermore, direct gram can predict the event of UTI.
Bahasa Indonesia Air Quality Parameters in the Maternity Room at Stella Maris Hospital Makassar: Parameter Kualitas Udara dalam Ruangan Bersalin di Rumah Sakit Stella Maris Makassar Palulun, Prajayanti; Yoeke Dewi Rasita; Nasrum Massi; Ilhamjaya Pattelongi; Sudirman Katu; Rizalinda Sjahril; Meutiah Ilhamjaya; Nadyah
Jurnal Midwifery Vol 6 No 1 (2024): FEBRUARY
Publisher : Prodi Kebidanan Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jmw.v6i1.41913

Abstract

Pendahuluan: Kualitas udara dalam lingkungan fasilitas kesehatan termasuk ruangan bersalin menggambarkan kondisi kesehatan lingkungan rumah sakit dan dianggap memiliki patogenisitas yang dapat menyebabkan infeksi yang berkaitan dengan rumah sakit. Angka kejadian infeksi banyak ditemukan dalam ruangan yang memiliki mikroorganisme di udara yang lebih tinggi seperti ruang bersalin. Metode: Penelitian ini menggunakan studi dekstriptif observasional dengan rancangan studi cross-sectional dengan melakukan pengamatan, pengukuran kualitas mikrobiologi udara dan identifikasi terhadap bakteri aerob di udara. Identifikasi koloni bakteri aerob yang tumbuh pada media blood agar menggunakan alat Matrix-Assisted Laser Desorption Ionization-Time Of Fight Mass Spectrometry (Maldi-Tof MS). Data yang diperoleh kemudian dilakukan analisis dengan menggunakan aplikasi Statistical for Social Science (SPSS). Analisis hubungan antara suhu dan kelembaban dalam ruangan terhadap nilai konsentrasi mikroorganisme di udara menggunakan uji korelasi Rank Spearman’s. Hasil: konsentasi mikroorganisme di udara dalam ruangan bersalin melebihi nilai standar baku berdasarkan Permenkes RI No.1204/Menkes/SK/X/ tahun 2004. Bakteri-bakteri aerob yang teridentifikasi di udara antara lain Staphylococcus arletae, Staphylococcus gallinarum, Bacillus cereus grup, dan Brevibacillus spp. Kesimpulan: Kelembaban udara sangat mempengaruhi pertumbuhan mikroorganisme di udara. Suhu ruangan pada nilai standar maksimal dan kelembaban dalam nilai standar minimal akan menjaga konsentrasi bakteri udara dalam ruangan memenuhi nilai standar mikroorganisme dalam ruangan.
Positive Blood Culture Results Predominantly Unrevealed among Adults Patients with Sepsis Hursepuny, Valentine; Baedah Madjid; Firdaus Hamid; Mochammad Hatta; Muh. Nasrum Massi; Rizalinda Sjahril; Yoeke Dewi Rasita; Lisa Tenriesa; Andi Rofian Sultan
Alami Journal (Alauddin Islamic Medical) Journal Vol 8 No 1 (2024): JANUARY
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/alami.v8i1.41777

Abstract

Sepsis is increasing every year and can be life-threatening. In the diagnosis of sepsis, it is challenging to identify the causative agent of infection. This research aims to determine the proportion of positive blood culture in sepsis patients at Hasanuddin University Hospital, Makassar. Blood cultures were collected from 98 adult patients with sepsis manifestations. This research design is cross-sectional with a descriptive observation approach. The data analysed in descriptive statistics were utilised to summarise patient demographics, treatments, and outcomes. Thirteen of 98 samples were positive for microbial pathogens (13.26%). The elderly group had the highest percentage (76.5%); eighty-seven patients (88.77%) were treated with antibiotic therapy prior to blood culture collection. Cephalosporins are the most administered antibiotics (72.4%). Administration of empirical therapy prior to blood culture collection may decrease the sensitivity of culture results. The culture of the infectious focus should be done to assist in the consideration of antimicrobial treatment.
Co-Authors A. Kaisar Adiwijaya Putra Andi Alfian Zainuddin Andi Nur Asrinawaty Andi Rofian Sultan Arif Santoso Baedah Madjid Dhian Karina Aprilani Hattah Dianawaty Amiruddin Evi Damayanti Firdaus Hamid Hursepuny, Valentine Ilhamjaya Pattelongi Lisa Tenriesa Meutiah Ilhamjaya Mochammad Hatta Muh. Nasrum Massi Nadyah Palulun, Prajayanti Sriwijaya Sriwijaya Sudirman Katu Yoeke Dewi Rasita