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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Amerta Nutrition The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy

Sri Estuningsih

Departemen Klinik Reproduksi Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680

Author-ID : 261079

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Veterinary Other

Published : 9 Documents Claim Missing Document

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Articles

Deteksi Dini Penyakit Tumor Sel Darah Myelosit Leukosis melalui Pemeriksaan Ulas Darah Dewi Ratih Agungpriyono; Hernomoadi Huminto; Sri Estuningsih; Aryani Sismin Satyaningtijas
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.708 KB)

Myelocytes blood cell tumor in chicken is a disease caused by retrovirus, avian leukosis virus subgroup J (ALV-J). The virus has the same group as human retrovirus (HIV) which caused AIDS, but the avian type possesses oncogenic properties, that could induce cell transformation and tumor formation. ALV-J stimulates the bone marrow's myelocytes and transforms them into tumor cell myelocytoma. The tumor cells then metastasis through the circulatory system causing myeloid leukosis and tumor cells accumulation in various internal organs or myelocytomatosis. This study was done on the base of the leukosis behavior of the tumor. The finding of the metastasis tumor cell in the blood smear examination is thought could be use as the diagnostic clue of the disease. Blood smear from sick chickens are collected and stained with some chemical staining substance such as may grunwald-giemsa, hematoxyllin eosin, periodic acid Schiff, congo red, toluidine blue, and sudan black B. Cytochemistry character of the blood cells was observed using light microscope. The result showed that myelocytes granules were best observed using hematoxyllin eosin, periodic acid Schiff, congo red and toluidine blue while may grunwald-giemsa, and sudan black B could not differentiate the granules. By this method, the field veterinarian will able to screen the suspected chicken flock for myeloid leukosis earlier than the occurrence of tumor formation.

Seleksi, Karakterisasi Morfologi, dan Identifikasi Aktinobakteri Penghasil Mananase Asal Hutan Tanah Jambi untuk Produksi Mananoligosakarida Rahayu Wulan; Rika Indri Astuti; Yaya Rukayadi; Sri Estuningsih; Anja Meryandini
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 2 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.2.279

As the world's largest producer of crude palm oil, Indonesia also produces high palm kernel cake (PKC) by-products. PKC has a high mannan content, so it can be used to produce prebiotic mannan-oligosaccharides (MOS). Enzymatic MOS production can be carried out using actinobacterial mannanase's microbiological approach. The HJ45B-1 isolate was the best isolate, with a peak enzyme production of 0,338 U/mL on the 10th day of incubation. The mannanase enzyme was stable in storage at 27°C. MOS production using 1% PKC substrate produced MOS with the best degree of polymerization (2-4) with incubation for 1-3 hours. Morphological characteristics and molecular identification based on the 16S rRNA gene indicated that the HJ45B-1 isolate was Streptomyces spp. Keywords: actinobacteria, mannanase, mannan-oligosaccharides, palm kernel cake, Streptomyces

APLIKASI MUTAN BERFLUORESENS UNTUK MEMPELAJARI KETAHANAN HIDUP, KOLONISASI DAN PENETRASI ISOLAT Cronobacter sakazakii SELAMA PENGERINGAN JAGUNG [Use of GFP Mutant to Study the Survival, Colonization and Penetration of Cronobacter sakazakii Isolates Duri Siti Nurjanah; Maggy T. Suhartono; Ratih Dewanti- Hariyadi; Sri Estuningsih
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 2 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Cronobacter sakazakii is a Gram-negative emerging pathogen regarded as causative agent of meningitis and necrotizing enterocolitis in certain groups of infants. In the previous research, thirty-two local isolates of C. sakazakii were obtained from various dried food products such as from corn starch, suggesting that they are able to survive drying. Some of the isolates were toxic. Green Fluorescent Protein (GFP) have been inserted to C. sakazakii and used as a marker for selective enumeration due to the ability of this protein to fluoresce under UV and to tolerate in ampicillin containing media. The objective of this study was to evaluate the survival, colonization and penetration of two isolates of C. sakazakii from dried food product during maize drying. The maize was challenged with mutants at a concentration of 105-106 CFU/g before drying. Maize drying was performed at temperature of 40ºC, 45ºC and 50ºC for 4, 6 and 8 days until the moisture content reached 14%. The totals of resistant drying mutants were counted every day onto ampicillin containing media by observing under UV light. The survival rate of C. sakazakii during drying was determined by the slope of linier regression from C. sakazakii survival curve. Isolates of FWHd16, the toxic strain of C. sakazakii, were more resistant to heat treatments in comparison to isolates of YRt2a, or the non toxic strain of C. sakazakii. Following fluorescence and scanning electron microscope observation, it is concluded that both isolates were colonizing on maize surface. These mutants were able to penetrate to the inner side of the grain by entering injured surface or pores at the tip cap of maize.

PAT-4 Histopathological Study of Mice (Mus musculus) Digestive Organs Treated with Alkali pH Water Sri Estuningsih; Vetnizah Juniantito; Yenny Rakhmawati
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Water is an important part of the body. As much as 50-60% of an adult's body weight consists of water. Water functions in the body include as a means of transporting substances, regulating body temperature, regulating body pH, forming body structures, solvents for the body's chemical reactions, and helping the body's mechanical functions, such as lubrication (Insel et al. 2004; Asamadi 2008). In the event of water deficiency, the body will become dehydrated which able to cause cell death to individual death (Stanfield and Hui 2008). Recently, there are alkaline pH drinking water products that are commercially popular that have pH around 8-10 (alkaline water). Some parties claim that alkaline water can help neutralize the level of acidity (pH) of blood due to free radicals, while also having micro cluster technology that can increase oxygen solubility. According to Shirahata et al. (2012) alkaline water is beneficial for health because it can suppress oxidative stress.This study aims to know the effect of alkaline pH drinking water reviewed through histopathological studies of the digestive organs of mice (stomach, intestine, and pancreatic exocrine glands).

PF-32 Radiography Assessment of Femoral Muscle and Bone Density in Rats as Response to Biodegradable Iron (Fe) Porous Implants Arlita Sariningrum; Mokhamad Fakhrul Ulum; Sri Estuningsih; Deni Noviana
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Iron (Fe) is considered as one group of metals that can be used as degradable metal implants (Schinhammer et al. 2010). Previous implantation studies have used porous Fe which purposes to increase the rate of degradation (Daud and Hermawan 2013). Changes in the condition of metal implants due to degradation and peri-implant muscle tissue reactions in the body can be observed using radiographic modality (Noviana et al. 2013). The aim of this study was to assess the radiographic density of implants, peri-implants and peri-implants-muscle as a response to Fe porous implants on the femur of the rats.

Preventing NSAID-induced Gastropathy: The Role of Mucus Cells to Prevent Aspirin-Induced Acute Gastric Mucosal Damage Chudahman Manan; Bambang Pontjo Priosoeryanto; Daldiyono Daldiyono; Sri Estuningsih; Min Rahminiwati
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 3, December 2011
Publisher : The Indonesian Society for Digestive Endoscopy

Background: Mucus is pre-epithelial gastric layer that may prevent damages due to direct contact between aspirin and gastric epithelial cells. The integrity of gastric mucosa and mucous cellular reaction may serve as primary and secondary prevention of extended aspirin-induced gastric mucosal damage. The aim of this study was to prove the function of mucus as defensive factor in rats. Method: The study was conducted in twenty white rats of the Sprague-Dawley strain at Department of Pathology and Clinical Reproduction, Bogor Agricultural University, between January and December 2008. The rat in the treatment group were given 400 mg aspirin diluted in aqua bidest through intra- gatric canules; while the control group received aqua bidest only once daily for 3 days. Necropsies, macroscopic and microscopic observation were performed by counting the number of Alcian blue- periodic acid Schiff-stained mucous cells at fundus/corpus and antrum/pylorus regions. Data analysis was performed using ANOVA and Duncan test. Results: The number of mucous cells with positive lesions in the treatment group was significantly different from the control group at both regions. There was no significant difference of negative lesions between treatment and control group at both regions. At antrum/pylorus region, there was no difference of negative lesions between treatment and control groups; however, both groups demonstrated significant difference of positive lesions in treatment group. Conclusion: In primary prevention for gastric mucosal lesions, there is no increasing number of mucous cells in normal mucosa. Increasing number of mucous cells is a secondary prevention against extended aspirin-induced gastric mucosal damage. Keywords: NSAIDs/ASA, mucus cells, gastric mucosal lesion, rat

Dyspepsia in Nonsteroidal Anti-inflammatory Drugs Gastropathy Chudahman Manan; Bambang Pontjo Priosoeryanto; Daldiyono Daldiyono; Sri Estuningsih; Min Rahminiwati
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 2, August 2011
Publisher : The Indonesian Society for Digestive Endoscopy

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) gastropathy is a common complication, which has characteristic symptoms of dyspepsia syndrome. Mostly, it includes epigastric discomfort with bloating and nausea. The aim of this study was to provide evidences that clinical symptoms of dyspepsia are related to macroscopic changes of gastric form in rats, which are expected to be applied in human. Method: The study was conducted in 20 white rats (Rattus norvegicus, Sprague-Dawley strain) at the Department of Pathology and Clinical Reproduction, Bogor Agricultural University between January and December 2008. The rats were divided to treatment group and control group and each group consisted of 10 rats. Acetyl salicylic acid (ASA/aspirin) was administered at 400 mg dose, diluted in distilled water and was given to the treatment group using gastric cannula, once daily for three days period; while the control group had received aquabidest only. Subsequently, necropsies were conducted for both groups, followed by macroscopic observation and measurement of sagittal and transversal diameter. Gastric incisions along the minor curvature were performed in both groups to recognize any macroscopic changes of gastric mucosa. ANOVA test was utilized for data analysis, which was followed by Duncan test when the results were significant. Results: Gastric diameters in treatment group with positive lesion were significantly different from the control group and the treatment group with negative lesion on anthrum/pylorus region, with p 0.05. Conclusion: Prominent gastric dilatation at anthrum/pylorus region found in the treatment group may become the initial cause and signs of dyspepsia in human. Keywords: NSAID gastropathy, dyspepsia, NSAIDs/aspirin, gastric dilatation

Kandungan Gizi dan Manfaat Air Kelapa terhadap Metabolisme Diabetes: Kajian Naratif: Nutritional Content and Benefits of Coconut Water for the Diabetes Metabolism: a Narrative Review Jeallyza Muthia Azra; Budi Setiawan; Zuraidah Nasution; Ahmad Sulaeman; Sri Estuningsih
Amerta Nutrition Vol. 7 No. 2 (2023): AMERTA NUTRITION (Bilingual Edition)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v7i2.2023.317-325

Background: Coconut water is one of the natural food products that is widely available around the world. This commodity provides nutritional content that could increase rehydration status and improve health. The application of coconut water to health, including improving type 2 diabetes metabolism, has gotten research interest in recent years. Objectives: This narrative review paper aimed to summarize several articles about the nutritional content and the mechanism of the potential bioactive component in coconut water to improve type 2 diabetes metabolism. Methods: This study design was a narrative review of several articles from five databases search: PubMed/MEDLINE, Scielo, PsycINFO, Microsoft Academic, and Google Scholar. Discussion: Coconut water includes several biologically active components such as protein, amino acid, fatty acid, mineral, vitamin, and phenolic compounds. The recent discovery of nutrient content, especially antioxidant properties, and arginine in coconut water, signifies a good potential in improving type 2 diabetes mellitus. Antioxidant and arginine altered blood glucose, glycated hemoglobin, serum creatinine, blood urea, albumin, albumin/globulin ratio, liver function enzyme, lipid profile, antioxidant status, and lipid peroxidation without any significant hepatocellular damage. Arginine enhanced the nitric oxide synthase activity in the liver and arginine levels in the plasma. In mammals, the arginine-nitric oxide system, which includes AMPK, cGMP, PGC-1α, and PI3K, can maintain blood glucose homeostasis, increase insulin sensitivity, and prevent diabetes-induced oxidative stress. Conclusion: Coconut water can help to improve metabolism in type 2 diabetes mellitus conditions through antioxidant activity and arginine content.

Potensi Penyebaran Infeksi Trichinella sp Akibat Konsumsi Daging Babi Hutan pada Karnivora Liar di Taman Margasatwa Ragunan Murtini, Sri; Satrija, Esdinawan Carakantara; Hendriana, Okta; Nugrahaeni, Mutiara; Edward, Syafri; Prasetyaningtyas, Wahono Esthi; Estuningsih, Sri; Satrija, Fadjar
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.%p

Trichinellosis merupakan penyakit zoonotik yang disebarkan oleh konsumsi daging mentah atau kurang matang yang mengandung larva cacing Trichinella sp. Keberadaan Trichinella spiralis di Indonesia telah teridentifikasi sejak masa kolonial Belanda, tetapi informasi mengenai kejadian trichinellosis pada manusia dan hewan di negara ini masih sangat terbatas. Sejumlah penelitian terkini mengindikasikan terjadinya peningkatan kasus trichinellosis pada hewan, khususnya babi dan babi hutan. Hal ini tidak hanya berpotensi mengancam kesehatan manusia, tetapi juga satwa liar karnivora yang mengonsumsi daging tersebut, termasuk satwa yang berada dalam penangkaran ex situ. Penelitian ini dilakukan untuk mendeteksi keberadaan larva Trichinella sp. pada daging babi hutan hasil buruan di Provinsi Bengkulu yang dimanfaatkan sebagai sumber pakan satwa liar karnivora di Taman Margasatwa Ragunan, Jakarta. Sebanyak 44 sampel daging babi hutan asal Bengkulu serta serum dari empat Harimau Bengala, dua Jaguar, dan satu Singa Afrika asal Taman Margasatwa Ragunan diperiksa menggunakan teknik Indirect Enzyme Linked Immunosorbent Assay (ELISA) untuk mendeteksi keberadaan antibodi terhadap Trichinella sp. Dari seluruh sampel daging yang diuji, tujuh (15,9%) menunjukkan hasil positif, 35 (79,5%) negatif, dan dua (4,5%) dubius. Pengujian serum Harimau Bengala menunjukkan satu (25%) seropositif, sedangkan sisanya dubious. Adapun pengujian pada serum Jaguar dan Singa Afrika menunjukkan hasil seronegatif. Harimau Bengala yang diuji merupakan individu yang lahir di Taman Margasatwa Ragunan dan hanya mendapat pasokan pakan berupa babi hutan asal Bengkulu. Oleh sebab itu, terdapat indikasi kuat bahwa infeksi Trichinella sp. pada babi hutan di Bengkulu dapat berpotensi memengaruhi kesehatan satwa liar karnivora yang ditangkarkan di Taman Margasatwa Ragunan, Jakarta.

Co-Authors A.S. Satyaningtijas Ahmad Sulaeman Anja Meryandini Arlita Sariningrum Bambang P. Priosoeryanto Bambang Pontjo Priosoeryanto Budi Setiawan Chudahman Manan Chudahman Manan Daldiyono Daldiyono Daldiyono Daldiyono Deni Noviana Dewi R. Agungpriyono Dewi Ratih Agungpriyono Dian Masyitha Eddy Widodo Edward, Syafri Faisal Amri Satrio Hendriana, Okta Hernomoadi Huminto Jeallyza Muthia Azra Maggy T. Suhartono Mokhamad Fakhrul Ulum, Mokhamad Ni Wayan Kurniani Karja Nugrahaeni, Mutiara Nurliana . Rahayu Wulan Rahminiwati, Min Ratih Dewanti -Hariyadi Rika Indri Astuti Robert Utji Satrija, Esdinawan Carakantara Siti Nurjanah sri murtini . Sugito Sugito Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Yaya Rukayadi Yenny Rakhmawati Zuraidah Nasution

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