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All Journal Jurnal Sain Veteriner Zuriat Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Aceh Journal of Animal Science Jurnal Sains Teknologi dan Lingkungan (JSTL) JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology)

Maskur Maskur

Fakultas Peternakan Universitas Mataram

Author-ID : 2444001

Agriculture, Biological Sciences & Forestry Computer Science & IT Earth & Planetary Sciences Education Public Health Veterinary

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Identifikasi Mutasi FecX Pada Gen BMP15 dan Pengaruhnya Terhadap Sifat Prolifik pada Kambing Lokal di Kabupaten Lombok Barat Rahmat Agus Hidayat; Sulaiman Ngongu Depamede; Maskur Maskur
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 1 No 1 (2015): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

The aims of this study were to identify the mutations of FecX gene in the local goats and to analyze its polymorphism as well as its influence on the prolific nature of the local goats in West Lombok Regency, Indonesia. The study was conducted in the Immunobiology Laboratory of Mataram University, using DNA isolated from 100 blood samples of local female goats which have given birth of once to three times. The methods used were PCR-RFLP method and the PCR products were digested with HinfI restriction enzyme (G|ANTC) then analyzed visually based on DNA banding patterns on 2% agarose gels. The frequency of allele and genotype obtained, were then analyzed through a comparison with the secondary data of litter size obtained from the local goat keepers information. The results showed that the gene mutation of FecXG produced two alleles: "wild-type" (+) sized of 110 bp and 31 bp, and the mutant allele (G) of 141 bp with the allele frequency of 0,965 and 0,035 respectively. Combinations of alleles in the gene BMP15 produced two genotypes, namely (a) genotype ++ (110 bp/110 bp) with a frequency of 0.93, with the average litter size of 1.59 Â± 0.319, and (b) genotype G + (141bp/110 bp), with a frequency of 0.07 and with the average litter size of 1.65 Â± 0.202. The results of this study indicated that mutation occurred in BMP15 gene, i.e. FecXG gene, the gene responsible for the prolificacy of animals studied. Furthermore there was a correlation between polymorphism of FecXG gene and the prolific nature of the local goats, which was predicted to lead the divergence in litter size of each local goat genotype

Evaluasi Penyimpanan Spermatozoa Ayam Pada Suhu 5⁰C, 26⁰C Dengan Pengencer Infuse NaCl, Glukosa 5% dan 10% Asnawi Asnawi; Maskur Maskur; Adji Santoso Dradjat
JURNAL SAINS TEKNOLOGI & LINGKUNGAN 2021: Special Issue, Oktober 2021
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v0i0.242

The purpose of this study were to compare the quality of spermatozoa stored at 26⁰C, 5⁰C using diluents of NaCl, 10% glucose and 5% glucose. The spermatozoa of a rooster was collected and divided into 6 parts, each 2 tubes diluted in a ratio of 1:1 using NaCl, Glucose5% and Glucose 10%, then each 3 tubes with different diluents were stored at 26⁰C and 5⁰C. Observations of motility, viability and abnormalities of spermatozoa were carried out half an hour, 1 hour after dilution, followed every 2 hours until the ninth hours. The results showed that spermatozoa stored for 9 hours at a temperature of 26⁰C with a physiological diluent of NaCl, 10% Glucose and 5% Glucose each were different (P, < 0.05) with motility 50 ± 0.0%, 42 ± 10.95. % and 34±8.94%, respectively. At storage temperature of 5⁰C for 9 hours, physiological NaCl, 10% glucose and 5% glucose were significantly different (P<0.05) with motility 58.00±10.95%, 46.00±8.94% and 38.00±, respectively. 10.95% in a row. The viability of spermatozoa at 26⁰C storage with 5% glucose diluent was better than 10% glucose and physiological NaCl (P<0.05), 58.93±1.27%, 42.93±1.48% and 33.43±1.27% , while the physiological NaCl diluent and 10% glucose were not significantly different (P>0.05). At 5⁰C storage the viability of spermatozoa in the three diluents was not significantly different, with values of Glucose 10%, Glucose 5% and physiological NaCl 52.57±5.15%, 52.21±5.02% and 48.14±8.09%, respectively. Spermatozoa abnormalities at storage temperature 26⁰C and 5⁰C for 9 hours using physiological NaCl diluent, 5% glucose and 10% glucose, were not significantly different and varied between 5 to 10%. Finally, it can be concluded that at room temperature storage less than 4 hours the quality of spermatozoa was better with 5% glucose diluent, while for cold storage beyond 4 hours the quality of spermatozoa with NaCl diluent was higher

KARAKTERISASI GEN -LAKTOGLOBULIN DAN HUBUNGANNYA DENGAN SIFAT PRODUKSI SUSU PADA SAPI HISSAR , Maskur; Cece Sumantri; , Muladno
Zuriat Vol 16, No 2 (2005)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v16i2.6773

Karakteristik gen-laktoglobulin dan hubungannya dengan sifat produksi susu pada 42 sapi Hissar dianalisis menggunakan teknik PCR-RFLP. Fragmen DNA-produk CPR dan hasil pemotongan menggunakan enzim restriksi Hae III dipisahkan menggunakan elektroforesis gel poliarkrilamid (PAGE) kemudian dideteksi dengan pewarnaan perak. Jumlah allel gen-laktoglobulin yang terdeteksi ada dua macam yaitu alel-A dan B dengan frekuensi masing-masing 0.19 dan 0.81. Uji signifikansi Chi-Square menunjukkan bahwa distribusi genotip gen-laktoglobulin dalam populasi sapi Hissar di Moyo Hilir Sumbawa-NTB berada dalam ketidakseimbangan dimana frekuensi genotip BB 69.05%; AB 23.81% dan AA 7.14%. Tingkat produksi susu memiliki korelasi yang kuat dengan genotip gen -laktoglobulin. Rata-rata produksi susu sapi Hissar dengan genotip AA, AB dan BB berturut-turut: 4.30 lt/hr; 3.11 lt/hr, dan 2.17 lt/hr.

Identification of Bmpr-1b and Bmp15 gene mutations in fat tail sheep Maskur .; Chairussyuhur Arman
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Fat tail sheep (FTS) is regarded as highly prolific local sheep and have been well adapted under tropical climate of Lombok island. BMPR-1B and BMP15 genes that controll reproductive traits such as ovulation rate and litter size in different type of sheep will be studied as candidate genes for prolific traits in FTS. These genes have been reported by various investigators have different prolificacy mechanism between several breeds of sheep, and it is very likely will occur in FTS. This study was designed to understand different prolificacy mechanism that occurred among breed of sheep. One hundred and forty FTS potential for twin and triplet scattered in Lombok Island, West Nusa Tenggara Province were screened to identify mutation of BMPR-1B and BMP15 genes using Forced PCR-RFLP method. Furthermore, the frequency of allel and genotype caused by mutation was measured in each gene. Results of the study indicated that mutation of FecXG at BMP15 gene resulted in two alleles, namely “wild-type”(+) Allel was 111 bp and 30 bp, and mutan allel (G) was 141 bp with frequency of 0.675 and 0.325. The combination of allel at BMP15 gen resulted in two genotipes, namely: ++ (111 bp/111 bp) and G+ (141 bp/111 bp) with frequency of 0.35 dan 0.65 in FTS. Mutation of FecB at BMPR-1B gen resulted in two allel, namely “wild-type”(+) Allel was 140 bp, and mutan allel (B) was 110 and 30 bp with frequency of 0.718 and 0.282. The combination of allel at BMPR-1B gen resulted in three genotipes, namely: BB (110 bp/110 bp), B+ (110 bp/140 bp), and ++ (140 bp/140 bp), its frequency was 0.11, 0.35 and 0.54 in FTS, respectively. Key words: Mutation, BMPR-1B, BMP15, Fat Tailed, Sheep

Substitusi Kuning Telur dengan Lesitin Kedelai sebagai Pengencer Semen dalam Mempertahankan Kualitas Spermatozoa Kerbau Penyimpanan 5°C Nifsu Sabaan; Chairussyuhur Arman; Enny Yuliani; Maskur Maskur
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 3 (2019): JITRO, September
Publisher : Universitas Halu Oleo

ABSTRAKPengencer konvensional berbasis kuning telur yang bersumber dari hewan membawa risiko kontaminasi mikroba dan menghalangi jarak pandang pemeriksaan spermatozoa di bawah mikroskop. Penelitian ini dilaksanakan` untuk menilai apakah substitusi kuning telur dengan lesitin kedelai di dalam pengencer berbasis-tris dapat mempertahankan kualitas spermatozoa kerbau lumpur pada penyimpanan 5°C selama 120 jam. Ejakulat dari 3 ekor kerbau umur 3-4 tahun dengan bobot badan 450-500 kg ditampung dengan menggunakan vagina buatan.Semen diencerkan dengan pengencer konvensional yang mengandung 20% kuning telur (kontrol) dan pengencer lesitin dengan konsentrasi1%, 2%, 3% dan 4%(perlakuan) dan disimpan pada 5°C selama 120 jam. Parameter spermatozoa dinilai pada interval 0, 72, dan 120 jam setelah semen yang diencerkan disimpan 5°C.Persentase motilitas dan spermatozoa normal tidak berbeda nyata (P<0,05) antara pengencer 1% lesitin dan kontrol. Namun pengencer 1% lesitin mampu mempertahankan kedua parameter tersebut setelah penyimpanan 5°C selama 120 jam. Persentase viabilitas, keutuhan membran dan tudung akrosom spermatozoa pada pengencer 1% lesitin dibandingkan pengencer kontrol lebih tinggi (P<0,05) dari pengencer perlakuan 2, 3, dan 4% lesitin. Disimpulkan bahwa pengencer 1% lesitin dapat menyubstitusi pengencer konvensional berbasis kuning telur untuk penyimpanan semen kerbau pada 5ºC selama 120 jam.Kata kunci: kuning telur, lesitin, pengencer, semen cair, semen kerbauABSTRACTConventional egg yolk-based diluent animal origin carries the risk of microbial contamination and interference with microscopic examination. This study was conducted to assess whether substitution of egg yolk with soy lecithin in tris-based diluents can maintain the quality of buffalo spermatozoa stored at 5°C for 120 h. Ejaculate from 3 buffaloes aged 3-4 years and body weight of 450-500 kg was collected using an artificial vagina. Semen was diluted with diluent containing 20% egg yolk (control) and diluent with concentrations of lecithin 1%, 2%, 3% and 4% (treatment). Spermatozoa parameters were assessed at intervals of 0, 72 and 120 h after the diluted semen was stored at 5°C for 120 h. The percentages of motility and normal spermatozoa did not differ significantly (P<0.05) between 1% lecithin and control diluents. However, 1% of lecithin was able to maintain both parameters after stored at 5°C for 120 h. The percentages of viability, membrane integrity and functional acrosome of spermatozoa in 1% lecithin were higher (P<0.05) than control and the other three treatment diluents. In conclusion, 1% of lecithin diluent could substitute conventional egg yolk-based diluent for the storage of buffalo semen at 5ºC for 120 h.Keywords: buffalo semen, egg yolk, lecithin, diluents, liquid semen

Pengaruh fasciolosis pada sapi bali berdasarkan pemeriksaan darah, serum glutamic pyruvic transaminase (SGPT), dan alkaline phosphatase (ALP) Amirullah Amirullah; Dradjat AS; Sriasih M; Maskur Maskur; Depamede SN; Kisworo D; Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Investation of Fasciola sp. On several species of cattle in Indonesia has occurred since a long time ago. Fasciolosis caused a high economical loss such as decreasing of carcass and organs, sometimes caused death of calves. The objectives of the research were to evaluate the effect of fasciolosis on Serum Glutamic Pyruvic Transaminase (SGPT), and the obstruction of bile duct by Alkaline Phosphatase (ALP) in Bali cattle suffered from fasciolosis. Faeces of Bali cattle raised in endemic areas of fasciolosis were examinated by sedimentation test to identify eggs per gram of faeces (EPG) of Fasciola sp. The blood samples of cattle which positive to fasciolosis were use to study the hematology by autoanalyser, SGPT and ALP. The results of research showed that from 89 cattle, 28 were infected by Fasciola sp. whithin of 7-115 EPG (min-max). From 20 fasciolosis cattle were indicated that MCHC of 90%, RBC of 30%, HB of 20%, and eosinophils of 2 % of cattle were lower than that of normal standard, while lymphocytes of 60%, MCV of 40%, ALP of 10% and SGPT of 5% of cattle were higher than that of normal standard. hematological appearances of cattle suffered from fasciolosis had macrocytichypochromic anemia, eosinopenia, and lymphocytosis. The increase of SGPT may be caused byhepatic cells destruction by young liver flukes, and the increase of ALP was caused by the obstruction of bile duct by mature liver flukes.

Correlation and regression analysis of the body measurements of the Doro Ncanga Buffalo (Bubalus bubalis) reared extensively in the Savanna of Mount Tambora Dompu Regency, Indonesia Husni Husni; Maskur Maskur; Chairussyuhur Arman
Aceh Journal of Animal Science Vol 3, No 1: July 2018
Publisher : Syiah Kuala University

The objectives of the present study were examined the correlation and regression of body measurements of the Doro Ncanga buffaloes in the Savanna of Mount Tambora Dompu Regency, Indonesia. A total of 339 animals were used in this study. In female, body weight (BW) was found to be significantly (p0.05) correlated with body length (BL), the degree of correlation increased from 0-6 (r=0.319) to 13-24 months (r=0.394), thereafter decreased at 36 months (r=0.160). The BW was not significantly correlated with height at wither (HW) for all 5 age groups. However, BW and heart girth (HG) were highly correlated (p0.01) between age groups 0-6 (r=0.967) and 36 months (r=0.978). The BL was significantly (p0.01) correlated with HW from 0-6 to 13-24 months of age, except for 25-36 months of age. The correlation between BL and HG was highly significant (p0.01) at 0-6 (r=0.427) and 13-24 (0.371) months of age, then decreased with the increase of age. The HW had no strong correlation with HG at all groups. Regression model of BW changes with HG was predictable with R2 values ranged from 0.896 to 0.957. In male, BW had a strong correlations with HG for age groups 0-6 (r=0.979) and 7-12 months (r=0.972). The BL and HW were not highly correlated with BW at all groups. However, BW was significantly (p0.01) correlated with HG for age groups 0-6 (r=0.979) and 7-12 months (r=0.972). Their R2 values were 0.958 and 0.945, respectively. A highly significant (p0.01) correlations were also observed between BL and HW for age groups 0-6 months (r=0.677)and 7-12 months (0.462). The R2 values were 0.458 and 0.214 for the two different age groups, respectively. The estimates of coefficient of determinations and predictive equations show that HG alone could be used to predict BW of female and male Doro Ncanga buffalo at different age groups.

Co-Authors Adji Santoso Dradjat Aminuyati Amirullah Amirullah Andilah Andilah Asnawi Asnawi Bulkaini (Bulkaini) Cece Sumantri Chairussyuhur Arman Chairussyuhur Arman Darus Ade Saputra Djoko Kisworo Dradjat AS Enny Yuliani F. Saputra, F. Febriantus Aguantara Husni Husni Jakaria Jakaria K. A. Paramitasari, K. A. Kisworo D Kurniasih Kurniasih Lalu Muhammad Kasip Lestari Lestari M. Muhsinin Made Sriasih N. Pratiwi, N. Nifsu Sabaan Nining Syarifulaya Nurul Huda P. P. Agung, P. P. R. Priyanto Rahma Jan Rahmat Agus Hidayat Rani Wulandani Sriasih M Sugeng Prasetyo Sulaiman Ngongu Depamede Sulaiman Ngongu Depamede Syahrudin Said Tapaul Rozi

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