Article Per Year (5 Year)
p-Index From 2020 - 2025
1.235
P-Index
This Author published in this journals
All Journal Jurnal Fitopatologi Indonesia Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agro Jurnal Pengabdian Pada Masyarakat Biosaintifika: Journal of Biology & Biology Education JURNAL PENGABDIAN KEPADA MASYARAKAT JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Jurnal Agro Wiralodra Proceedings Series on Physical & Formal Sciences Jurnal Ilmiah Media Agrosains
Loekas Soesanto
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Library & Information Science Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

Published : 12 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 12 Documents
Search

 1   2 
Raw Secondary Metabolites of Trichoderma harzianum T10 in Tapioca Flour Towards Cucumber Damping-off Loekas Soesanto; Hidayatul Ilahiyyah; Endang Mugiastuti; Abdul Manan; Rostaman Rostaman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24567

Abstract

Trichoderma harzianum is effective for controlling soil-borne pathogenic fungi and producing secondary metabolites. When applied in the field, the raw secondary metabolites are quickly decreased directly by sunlight. One strategy to avoid degradation is the use of tapioca fluor liquid formula for biological control agents. This research aimed to obtain the most effective concentration of tapioca flour in development of raw secondary metabolites of Trichoderma harzianum T10, its effect on damping-off and growth of cucumber. This research was carried out at the screen house and the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University from September 2017 up to January 2018. The study was conducted in two stages, i.e., in vitro and in planta. The in vitro stage used completely randomized design with five repetitions and five treatments consisted of T. harzianum T10 in Potato Dextrose Broth, and in 0.5; 1; 1.5; and 2% of tapioca flour media. In in planta, randomized block design was used with five repetitions and six treatments consisted of control, T. harzianum T10 in PDB, and in 0.5; 1; 1.5, and 2% of tapioca flour media. Variables observed were density of conidia, disease incubation period, disease incidence, AUDPC, maximum growth potential, germination ability, plant height, canopy fresh weight, root length, and fresh root weight. Result of the research showed that the highest conidial density (1.23 x 107 conidia mL-1) of T. harzianum T10 was found in 2% tapioca flour with an increase of 63.28% compared to the PDB. The tapioca flour of 1 and 2%, and PDB could suppress the disease incidence by 81.82%. The lowest AUDPC was at 2% tapioca flour. The raw secondary metabolites could not delay the incubation period significantly and increase cucumber plant growth. The novelty is the use of antagonistic fungi in terms of raw secondary metabolites and the discovery of tapioca flour with the right concentration to produce high conidia density and high raw secondary metabolites. The benefits are to find other cheaper ingredients in promoting antagonistic fungal growth and the use of antagonistic fungal bioactive compounds to control plant pathogen
EKSPLORASI DAN UJI VIRULENSI BAKTERI Bacillus sp. ENDOFIT JAGUNG TERHADAP PENYAKIT BUSUK PELEPAH JAGUNG Arum Saputri; Loekas Soesanto; Abu Umayah; Agus Sarjito
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 22 No 2 (2020)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.22.2.70-78

Abstract

[EXPLORATION AND VIRULENT TEST OF MAIZE ENDOPHYTE Bacillus sp. AGAINST MAIZE SHEATH BLIGHT]. Capability of endophytic bacterial, effectivity, and its effect on R. solani. and on maize seedlings growth were investigated from April 2018 to January 2019. Exploration of endophytes bacteria in maize was taken from Banyumas Regency (Sumbang, Kembaran, Baturraden) and Purbalingga Regency (Padamara, Bojongsari, Pratin). Taking plant samples using Purposive Random Sampling and Diagonal Sampling methods. Completely randomized design was used in in vitro test with 16 treatments repeated twice. Completely randomized block design was used in in planta experiment with 5 treatments repeated 5 times. The treatment consisted of control, fungiside (mankozeb), and 2 isolates of endophytes bacteria performing the best in vitro result. Variables observed included characteristics of endophytic bacteria and pathogenic fungi, inhibition diameter, incubation period, disease intensity, incidence of disease, AUDPC, plant height, leaf number, root length, plant fresh weight, canopy fresh weight, and root fresh weight. Resultshowed that the exploration obtained 15 endophytic Bacillus sp. isolates. The PD A.4 and BK A.1 isolates werw able to inhibit the growth of pathogenic fungi in-vitro by 56.93 and 51.5%, respectively. The soaking treatment using BK A1 was able to reduce disease intensity by 59.377%, and AUDPC value 34.19%. Endophytic bacteria influence plant height, plant fresh weight, canopy fresh weight, and fresh weight of roots respectively as 89.17 cm, 126.06 g, 106.67 g and 19.4 g.
Co-Authors abdul manan Abu Umayah Agus Sarjito Agus Suroto Arum Saputri Dede Yusup Ziaulhak Dwimartina, Fina Endang Mugiastuti Hening Kurniasih Henky Setyawan Norandika Wahyu Hidayatul Ilahiyyah Kustantinah Kustantinah Muhamad Bahrudin Ni Wayan Anik L Nur Prihatiningsih Rostaman Ruth Feti Rahayuniati Tamad wahyu febriyono