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All Journal Jurnal Fitopatologi Indonesia Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agro Jurnal Pengabdian Pada Masyarakat Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education JURNAL PENGABDIAN KEPADA MASYARAKAT JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Jurnal Agro Wiralodra Proceedings Series on Physical & Formal Sciences Jurnal Ilmiah Media Agrosains
Loekas Soesanto
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Raw Secondary Metabolites of Trichoderma harzianum T10 in Tapioca Flour Towards Cucumber Damping-off Loekas Soesanto; Hidayatul Ilahiyyah; Endang Mugiastuti; Abdul Manan; Rostaman Rostaman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24567

Abstract

Trichoderma harzianum is effective for controlling soil-borne pathogenic fungi and producing secondary metabolites. When applied in the field, the raw secondary metabolites are quickly decreased directly by sunlight. One strategy to avoid degradation is the use of tapioca fluor liquid formula for biological control agents. This research aimed to obtain the most effective concentration of tapioca flour in development of raw secondary metabolites of Trichoderma harzianum T10, its effect on damping-off and growth of cucumber. This research was carried out at the screen house and the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University from September 2017 up to January 2018. The study was conducted in two stages, i.e., in vitro and in planta. The in vitro stage used completely randomized design with five repetitions and five treatments consisted of T. harzianum T10 in Potato Dextrose Broth, and in 0.5; 1; 1.5; and 2% of tapioca flour media. In in planta, randomized block design was used with five repetitions and six treatments consisted of control, T. harzianum T10 in PDB, and in 0.5; 1; 1.5, and 2% of tapioca flour media. Variables observed were density of conidia, disease incubation period, disease incidence, AUDPC, maximum growth potential, germination ability, plant height, canopy fresh weight, root length, and fresh root weight. Result of the research showed that the highest conidial density (1.23 x 107 conidia mL-1) of T. harzianum T10 was found in 2% tapioca flour with an increase of 63.28% compared to the PDB. The tapioca flour of 1 and 2%, and PDB could suppress the disease incidence by 81.82%. The lowest AUDPC was at 2% tapioca flour. The raw secondary metabolites could not delay the incubation period significantly and increase cucumber plant growth. The novelty is the use of antagonistic fungi in terms of raw secondary metabolites and the discovery of tapioca flour with the right concentration to produce high conidia density and high raw secondary metabolites. The benefits are to find other cheaper ingredients in promoting antagonistic fungal growth and the use of antagonistic fungal bioactive compounds to control plant pathogen
EKSPLORASI DAN UJI VIRULENSI BAKTERI Bacillus sp. ENDOFIT JAGUNG TERHADAP PENYAKIT BUSUK PELEPAH JAGUNG Arum Saputri; Loekas Soesanto; Abu Umayah; Agus Sarjito
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 22 No 2 (2020)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.22.2.70-78

Abstract

[EXPLORATION AND VIRULENT TEST OF MAIZE ENDOPHYTE Bacillus sp. AGAINST MAIZE SHEATH BLIGHT]. Capability of endophytic bacterial, effectivity, and its effect on R. solani. and on maize seedlings growth were investigated from April 2018 to January 2019. Exploration of endophytes bacteria in maize was taken from Banyumas Regency (Sumbang, Kembaran, Baturraden) and Purbalingga Regency (Padamara, Bojongsari, Pratin). Taking plant samples using Purposive Random Sampling and Diagonal Sampling methods. Completely randomized design was used in in vitro test with 16 treatments repeated twice. Completely randomized block design was used in in planta experiment with 5 treatments repeated 5 times. The treatment consisted of control, fungiside (mankozeb), and 2 isolates of endophytes bacteria performing the best in vitro result. Variables observed included characteristics of endophytic bacteria and pathogenic fungi, inhibition diameter, incubation period, disease intensity, incidence of disease, AUDPC, plant height, leaf number, root length, plant fresh weight, canopy fresh weight, and root fresh weight. Resultshowed that the exploration obtained 15 endophytic Bacillus sp. isolates. The PD A.4 and BK A.1 isolates werw able to inhibit the growth of pathogenic fungi in-vitro by 56.93 and 51.5%, respectively. The soaking treatment using BK A1 was able to reduce disease intensity by 59.377%, and AUDPC value 34.19%. Endophytic bacteria influence plant height, plant fresh weight, canopy fresh weight, and fresh weight of roots respectively as 89.17 cm, 126.06 g, 106.67 g and 19.4 g.
Potensi Bacillus subtilis sebagai Agen Pengendali Hayati terhadap Pyricularia oryzae pada Tanaman Padi silfina fara amelia; Meylita Filia Pramesti; Sekar Putri Arista; Azika Saputri Haryanto; Loekas Soesanto
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 10 No. 2 (2026): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 10 (2) Juni 2026
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v10i2.17642

Abstract

Penyakit blas yang disebabkan oleh jamur Pyricularia oryzae merupakan salah satu ancaman paling destruktif terhadap produktivitas padi secara global, termasuk di Indonesia. Infeksi patogen ini pada berbagai fase pertumbuhan tanaman dapat menyebabkan kerugian ekonomi yang signifikan hingga kegagalan panen total. Penelitian ini bertujuan untuk mengevaluasi efektivitas bakteri Bacillus subtilis sebagai agen pengendali hayati yang ramah lingkungan dalam menekan pertumbuhan Pyricularia oryzae. Metode penelitian meliputi isolasi bakteri dari rhizosfer, uji antagonis secara in vitro menggunakan teknik cawan ganda (dual culture), serta aplikasi suspensi bakteri pada tanaman padi di rumah kaca untuk mengamati intensitas penyakit. Hasil penelitian menunjukkan bahwa B. subtilis mampu menghambat pertumbuhan radial jamur patogen secara signifikan dengan persentase penghambatan mencapai 72% pada hari ketujuh. Aplikasi pada tanaman padi juga terbukti efektif menekan gejala blas serta meningkatkan parameter pertumbuhan tanaman seperti tinggi dan jumlah anakan. Dapat disimpulkan bahwa B. subtilis memiliki potensi besar sebagai alternatif biopestisida yang efektif untuk pengendalian penyakit blas padi secara berkelanjutan. Kata kunci: Bacillus subtilis, Pyricularia oryzae, penyakit blas, uji antegonisme, biopestisida
Potential of Beauveria bassiana Controlling Nilaparvata lugens for Food Security: A Review Article Fijar Muhamad Fathurohman; Raisa Shakila Parsa; Indah Lestari; Farica Falihah; Loekas Soesanto
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11885

Abstract

The Brown Planthopper (Nilaparvata lugens Stål) is a strategic pest threatening the stability of national rice production. Farmers' dependence on synthetic insecticides has triggered pest resistance and environmental degradation, making the utilization of biological agents such as the entomopathogenic fungus Beauveria bassiana an urgent alternative solution. This study aims to comprehensively analyze the potential, mechanisms, and effectiveness of B. bassiana in controlling N. lugens. The method used is a Systematic Literature Review (SLR) by examining experimental articles from accredited national journals and reputable international journals published between 2018 and 2025. Results of data synthesis indicate that B. bassiana has a very high level of pathogenicity (nymph mortality >78%), especially when using local isolates cultured on corn media. However, its effectiveness is highly influenced by abiotic factors, where conidial viability is optimal at 25°C and drops sharply at extreme temperatures (34°C). Recent findings prove that B. bassiana can colonize as an endophyte within rice tissues, suppressing planthopper fecundity while simultaneously inducing systemic plant resistance. Additionally, this fungus is proven compatible and synergistic when combined with sublethal insecticide doses. It is concluded that B. bassiana is effective as both a contact biopesticide and an internal protection agent within Integrated Pest Management.  
Co-Authors abdul manan Abu Umayah Agus Sarjito Agus Suroto Arum Saputri Azika Saputri Haryanto Dede Yusup Ziaulhak Dwimartina, Fina Endang Mugiastuti Farica Falihah Fijar Muhamad Fathurohman Hening Kurniasih Henky Setyawan Norandika Wahyu Hidayatul Ilahiyyah Indah Lestari Kustantinah Kustantinah Meylita Filia Pramesti Muhamad Bahrudin Ni Wayan Anik L Nur Prihatiningsih Raisa Shakila Parsa Rostaman Ruth Feti Rahayuniati Sekar Putri Arista silfina fara amelia Tamad wahyu febriyono