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POTENSI LIMBAH KULIT PISANG KEPOK (Musa paradisiaca) SEBAGAI BAHAN BAKU PEMBUATAN ASAM ASETAT MENGGUNAKAN BERBAGAI MACAM STARTER Ilham '; Itnawita '; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Kepok banana  (Musa  paradisiaca)  peel  waste contain sugar that can  be  changed  to acetic acid using Effective Microorganism (EM-4), kombucha (KO), cassava yeast (RT) and instant yeast  (RI)  (commercial  starter). The Commercial  starter  contains variety of bacteria and yeasts  with different content of microorganism  that will influence  the amount of ethanol  produced.  The aim of this study  to  determine the  potential of kepok banana peel  waste used as the main material  in synthesis  of acetic acid using commercial  starter.  Based on the  fermentation  results  using  the four starter,  cassava yeast  has  a  good  ability  to  be  fermented.  The acetic acid formation was  obtained  under condition which substrate and starter amount are 80% w/v and 5 grams respectively and fermentation  time  4  day.  Based on these conditions  it was found that the amount of organic acid total  determined  by  titrimetric  method  is  4.712±0.066%  and  acetic  acid formed which was determined by Ion Chromatography method is 0.7507± 0.0538%.
THE BACTERIOCIN ANTIMICROBIAL TEST ACTIVITY OF PROBIOTIC BACTERIA ISOLATED FROM GIANT PRAWNS (Macrobrachium rosenbergii) Popy Wardani; Feliatra '; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiotic bacteria isolated from giant prawns (Macrobrachium rosenbergii). The method used was experiment method with tested the inhibition against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri). The results showed that bacteriocin capable of inhibiting the growth of pathogenic bacteria of P. stutzeri, A. hydrophila and V. alginolyticus. The best isolates obtained from results of this research was UG4 with clear zones ranging from 7.2 to 11.4 mm and the inhibition activity of 1476.53 mm²/mL after fragmentation with (NH4)2SO4 against pathogenic bacteria P. stutzeri was incubated at room temperature (37°C). This inhibition was caused by the presence of antimicrobial compounds that are produced by probiotic bacteria, namely bacteriocin. For isolates UG2 codes, the bacteria was resistant in which characterized by the formation of bluring zone around the paper disc.Key words: Antimicrobial activity, probiotics, bacteriocins, giant prawns
Secondary Metabolite Test for Heterotrophic Bacteria from Siak River Estuary Against Pathogenic Bacteria Adolf Hiskia Nainggolan; Feliatra Feliatra; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

ABSTRACT   Secondary metabolites are a molecule or product of metabolites produced by the metabolic processes of microorganisms where the metabolite products are not a basic necessity of living microorganisms and for growth, but secondary metabolites can also function as emergency nutrients to survive. Marine bacteria including heterotrophic bacteria are capable of producing secondary metabolites. This study aims to determine the ability of secondary metabolite production of heterotrophic bacteria to inhibit the activity of pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp). In this study using the experimental method. 10 Heterotrophic bacterial isolates obtained from a collection of marine microbiology laboratories were cultured on NB media ± 3 days until the media was cloudy and harvest during stationary times, then extracted secondary metabolites from the media and shells using Etyl Acetate. The secondary metabolite extract will be tested against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp). Based on the results of the antagonism test conducted, 10 heterotrophic bacterial isolates were able to inhibit the growth of all three pathogenic bacteria. Based on the antagonism test, the highest inhibitory value was Bacillus cereusspecies (KM489154.1) with an average diameter of about 10.08 mm, while the inhibition power which was classified as the lowest or categorized as moderate was found in the Bacillus safensis species (KY495152.1 ) with an average diameter of around 7.33 mm. The ability to inhibit pathogenic bacteria is suspected because this heterotrophic bacterial species produces antibiotic compounds. Keyword :Secondary metabolites,  Heterotrophic Bacteria, Antagonism test
TEST OF BACTERIOCIN ANTIMICROBIAL ACTIVITY OF PROBIOTIC BACTERIA ISOLATED FROM TIGER SHRIMP (Penaeus monodon Fabricus) Anita R Sidabutar; Feliatra '; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin antimicrobial. Research to analyzed the bacteriocin of 5 probiotic bacterial from isolates tiger shrimp (Penaeus monodon Fabricus). The bacteriocin product contained in 2 forms, that i.e. without precipitation (extract bacteriocin) and with precipitation (fragment bacteriocin) used ammonium sulfate (NH4)2SO4. Bacteriocin will be tested activity against pathogen bacterial that cause fish disease was is Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri. Inhibitory activity test conducted using paper disc diffusion method. Inhibitory activity bacteriocin with precipitation showed inhibition was higher the biggest than without precipitation. Fragment bacteriocin (BC) with precipitation had inhibitory activity or AU (Activity Unit) i.e. 881,71 mm2/ml at UWH9 isolate toward bacteria test A. hydrophila and P. stutzeri. The biggest Inhibitory activity of extract bacteriocin (CR) without precipitation was 365,65mm2/ml at UWH10 isolate toward bacteria test P. stutzeri.Keywords: Bacteriocin, Pathogen bacterial, Precipitation, Inhibitory activity
Produksi Enzim Lakase Oleh jamur Trichoderma Asperellum Lbkurcci Dalam Bioreactor Tray Menggunakan Variasi Ukuran Substrat Jerami Padi Dan Induser CuSO4 Pada Fermentasi Kultur Padat Gustina Gustina; Sri Helianty; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Laccase is one of the ligninolityc enzymes that capable to degrade lignin. This ability can be used for the pretreatment of lignocellulosic materials in the bioethanol production and lignin degradation in pulp. There are diverse sources of laccase producing like fungi, plants and bacteria. In this research, the production of lacase enzyme using Trichoderma asperellum LBKURCC1 with bioreactor tray using solid state fermentation (SSF) method with rice straw substrate. The purpose of this study was to determine the effect of rice straw size and the addition of CuSO4 concentration to the highest production of lacase enzyme by Trichoderma asperellum LBKURCC1. Fermentation is carried out with variation of time 5, 6, 7, 8, 9 and 10 days with fermentation temperature ± 30ºC, substrate size ± 0.5 cm, 1.5 cm, and 3.0 cm with substrate thickness at tray ie 3 cm acetate buffer solution pH 5,5 and addition of CuSO4 0.5 g/l. Variations for the addition of CuSO4 concentration 0 g/l, 0.50 g/l and 1 g/l with substrate thickness on tray were 3 cm, size of rice straw 0.5 cm and acetate buffer solution pH 5.5. Small size can provide the highest value and the added concentration of CuSO4 given can increase the activity of the resulting lacase enzyme. However, too high CuSO4 concentrations result in decreased lacase enzyme activity. The results showed that the highest lacase enzyme activity was obtained on the size of rice straw ie 0.5 cm and 7 days of fermentation time with an average of 19.27 U/L and the highest lacase enzyme activity was obtained on CuSO4 0.50 g/l and fermentation time 7 day with an average of 19.27 U/L.Keywords: Inducer, Laccase, Rice straw, Solid state fermentation, Trichoderma asperellum.
Produksi Enzim lakase Oleh Jamur Tricoderma Asperellum Lbkurcci Secara Fermentasi Padat Pada Bioreactor Tray Muhamad Fajri; Sri Helianty; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Laccase enzyme is classified as oxidoreductase enzyme; i.e. an enzyme that can oxidize phenolic compounds and reduce oxygen molecules into water molecules. An enzyme production process using fungi through solid fermentation has more advantages compared to the one through submerged fermentation since it has the processing condition as if the fungi grow in nature. This research is aimed at investigating the process of laccase enzyme production by Trichoderma asperellum LBKURCC1 in bioreactor tray through solid state fermentation using rice straw substrates. The objective of this research is to figure out the influence of bed depth and fermenting time on laccase enzyme production by Trichoderma asperellum LBKURCC1. The variables of the research are the bed depth of 1.5, to 4.5 cm and fermentation time of 5, to 10 days. The research findings show that the peak of laccase enzyme activity was obtained at 3.0 cm bed depth and 7 day fermentation time. Increasing the depth of the bed can increase the activity of laccase enzyme produced. However, increasing too much depth of the bed can cause the decrease of laccase enzyme activity. Besides, the fermentation time over 7 days can also decrease the laccase enzyme activity for the amount of the water contained in the bed is drying up.Keywords: bioreactor tray, laccase enzyme, rice straw, Trichoderma asperellum LBKURCC1
Penerapan teknologi biopori dalam pencegahan banjir dan kekeringan yang sekaligus pembuatan biokompos di Kelurahan Delima Kecamatan Tampan Pekanbaru Andi Dahliaty; Yuana Nurulita; Titania Tjandrawati Nugroho; Sri Helianty
Unri Conference Series: Community Engagement Vol 1 (2019): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.1.255-261

Abstract

Flooding is a problem that almost every year affects urban and rural areas. Generally, in urban areas, floods are caused more by the lack of smooth flow of water (sewage) due to waste dumped into the water stream and the reduction of water catchment areas. The Delima Subdistrict of Tampan Subdistrict has a simple housing area that has a limited home yard measuring around 7m x13m up to 10m x 15m per house unit. To meet clean water needs, this area uses bore wells which over time will reduce underground water reserves. The housing area is sometimes disemenised, which results in a lack of infiltration of rain water so that it is prone to flooding and drought. To overcome this, the community can apply biopore infiltration technology. This service activity aims to introduce biopore infiltration technology and invites the community to apply it and make biocompost from the infiltration hole. The method of service activities was carried out with material delivery techniques and discussions as well as demonstrations or practices in making biopori infiltration holes as an effort to overcome floods and droughts in settlements around the Delima Village, Tampan District, Pekanbaru. From the results of this activity it is known that the target community seemed enthusiastic about the material presented and there was an increase in the knowledge and insights of the participants about biopori absorption technology by around 80%.
Produksi Biokompos Melalui Teknik Mini Biopori di dalam Wadah Bekas Ramah Lingkungan Untuk Tanaman Hortikultura Dahliaty, Andi; Sophia, Halida; Azlina, Nur; Siregar, Siti Saidah; Susilawati, Susilawati; Nugroho, Titania Tjandrawati
Unri Conference Series: Community Engagement Vol 6 (2024): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.6.247-253

Abstract

Mini biopori is an innovative and efficient technique for producing biocompost. This technique utilizes used containers that are environmentally friendly, allowing the community to independently process household organic waste. In addition to reducing waste, this technique also produces fertilizer that is beneficial for horticultural plants. The purpose of this community service activity is to provide knowledge and insights to the community about the application of mini biopori technology in used containers for horticultural plant production, enhance the community's skills in applying mini biopori technology, utilize the biocompost produced from biopori infiltration holes for planting horticultural crops, and assist the community from an economic aspect (increasing community income) by using simple and inexpensive materials to produce high-value horticultural plants. This activity is conducted in Sungai Berbari Village, Pusako District, Siak Regency. The methods of this community service consist of several stages, including preparation, training through lectures and discussions, practical implementation of the mini biopori technique, and evaluation, which considers the conditions before and after the training activities. The results of this activity indicate that the participants have a better understanding of the biocompost production process and the benefits of the mini biopori technique. All participants (100%) considered the mini biopore technique training to be very beneficial, indicating that this training has successfully provided added value to the participants. A total of 70% of the participants showed an interest in applying the mini biopore technique in their homes. The mini biopore technique is considered effective in managing organic waste, producing beneficial fertilizer, and has the potential to reduce dependence on chemical fertilizers. Additionally, implementing this technique can lower agricultural production costs, increase crop yields, and contribute to long-term economic well-being in the community.
BIODEGRADATION OF USED BABY DIAPERS USING CELLULOLITIC FUNGUS AND BACTERIA WITH SOLID FERMENTATION Novianty, Riryn; Dahliaty, Andi; Nasution, Nurul Iflah; Haryati, Haryati
Jurnal Inovasi Pendidikan dan Sains Vol 1 No 1 (2020): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

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Abstract

Diapers are made by cotton and pulp containing cellulose that can be used as a substrate in the production of cellulose enzymes. The purpose of this research is to determine the ability of cellulolytic fungi Trichoderma asperellum LBKURCC1 and cellulolytic bacteria S-22 to degrade the used diapers containing urine by solid fermentation for 10, 20, and 30 days. The activity of crude extract of cellulose enzyme was observed with CMC 2% as substrate at pH 5,5 (Trichoderma asperellum LBKURCC1) and pH 7 (bacteria S-22 isolate), incubation temperature 40ºC during 30 minutes by using Nelson Somogyi’s method. The result showed that used diapers can be used as substrate for cellulose enzyme production by fungi Trichoderma asperellum LBKURCC1 with the highest activity of cruide extract cellulose enzyme obtained at 10th days solid fermentation of (1,891 ± 1,453) × 10-3 U / mL and by bacteria S-22 isolate at 20th days solid fermentation of (2,854 ± 0,019) × 10-3 U/mL. It can be concluded that bacteria S-22 isolate would degrade the used diapers better than fungi Trichoderma asperellum LBKURCC1. The result of this research compared to quality standards compost of SNI: 19-7030-2004 have not fulfilled, so it can not be used as compost.