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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia JURNAL KIMIA SAINS DAN APLIKASI BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Biology, Medicine, & Natural Product Chemistry Jurnal Kimia Riset Al-Kimia Journal of Tropical Crop Science Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Jurnal Kartika Kimia Jurnal Riset Sains dan Kimia Terapan Journal of Applied Materials and Technology Jurnal Abdimas Kartika Wijayakusuma Jurnal Pusat Inovasi Masyarakat Indonesian Journal of Chemical Studies (Indones. J. Chem. Stud.) Gunung Djati Conference Series Biodiversitas Journal of Biological Diversity
Deden Saprudin
Department Of Chemistry, Faculty Of Mathematics And Natural Science, Bogor Agricultural University,Jl. Raya Darmaga, Bogor, Indonesia
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Influence of Potassium Permanganate on Physicochemical Quality Attributes of Oil Palm (Elaeis guineensis Jacq.) Fresh Fruit Bunches Imelya Ramanis; Deden Saprudin; Sudradjat Sudradjat
Jurnal Ilmu Pertanian Indonesia Vol. 31 No. 2 (2026): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.31.2.269

Abstract

The physical and chemical properties of fresh fruit bunches (FFB) determine the quality of crude palm oil (CPO). The damage to the palm oil fruit accelerates the hydrolysis process, increasing the free fatty acid (FFA) content while decreasing the oil concentration. Damage is caused by the fruit's postharvest procedure, such as transportation time to the manufacturer and collection delays. The processing latency must be appropriate so that FFB damage can be reduced with potassium permanganate (KMnO4). The purpose of this study was to determine the effect of KMnO4 on the physical and chemical properties of palm oil FFB, as well as the relationship between the observed variables. The FFB samples were taken from the IPB-Cargill Palm Oil Teaching Farm in Jonggol Bogor, Indonesia. This study employed a randomized complete block design. The experiment included four different concentrations of KMnO4 (0%, 0,025%, 0,050%, and 0,075%). Each treatment was reproduced three times. The results showed that using KMnO4 could preserve the physical quality of palm oil fruit in terms of weight loss, fruit fall, respiration rate, and microbiological content. The chemical quality of fruit is determined by its moisture content and FFA concentration. The optimal KMnO4 concentration was 0.075%. The correlation analysis results show that the observed variables have a positive association. Variables with a strong relationship included weight loss and fruit fall, weight loss and several microbes, weight loss and number of microbes, water content and number of microbes, and FFA and number of bacteria. Keywords: crude palm oil, fresh fruit bunches, potassium permanganate, weight loss
CHARACTERIZATION OF STREPTOMYCES sp. 45I-3 XYLANASE Anja Meryandini; Deden Saprudin; Prima Agung Prihandono; Alina Akhdiya; Trio Hendarwin
BIOTROPIA Vol. 14 No. 2 (2007): BIOTROPIA Vol. 14 No. 2 December 2007
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.57 KB) | DOI: 10.11598/btb.2007.14.2.16

Abstract

Streptomyces sp 45I-3 producing xylanase was isolated from Kalimantan soil sample. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase has an optimum condition at pH 5 and 50 oC.  Crude xylanase have half-life time of 2 hours 9 minute at 50 oC, whereas purified xylanase has half-life time of 12 hours. The molecular mass of purified xylanase was determined to be 43.2 and 39.2 kDa.
GROWTH, PHOTOSYNTHESIS AND PROLINE ACCUMULATION OF METAL-ACCUMULATOR WEEDS Hamim Hamim; Rani Apriani; Deden Saprudin; Yohana C Sulistyaningsih
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.267 KB) | DOI: 10.11598/btb.0.0.0.1200

Abstract

Intensive use of heavy metals, particularly lead and mercury, in industrial activities and mining extraction has caused the widespread distribution of these contaminants, thereby threatening the environment. Consequently, the growth and several physiological responses of five metal-accumulator weed species were examined in response to mercury (Hg) and lead (Pb) treatments. The weed species Brachiaria mutica, Cyperus kyllingia, Ipomoea aquatica, Mikania micrantha, and Paspalum conjugatum were grown in water culture using half-strength Hoagland's solution and subjected to Hg(NO₃)₂ and Pb(NO₃)₂ at concentrations of 0, 0.25, and 0.5 mM for 3 weeks. Plant growth, photosynthesis, lipid peroxidation, and proline content were observed. Both Hg and Pb significantly reduced plant growth, but the decrease was more pronounced under Hg treatment than under Pb. Hg dramatically reduced the photosynthetic rate under different photosynthetic photon flux densities, indicating that Hg up to 0.5 mM damaged the photosynthetic apparatus in almost all species except I. aquatica. Hg and Pb treatments caused a dramatic increase in leaf MDA content, which was associated with a significant decrease in chlorophyll content. Most species were tolerant to Pb up to 0.5 mM except M. micrantha, while only C. kyllingia and I. aquatica were tolerant to Hg treatment up to 0.5 mM. Hg treatment also induced higher proline accumulation in leaves, although without a clear increment pattern among species, suggesting that proline may function as a stress alarm rather than as a tolerance indicator.
Characterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16 ANJA MERYANDINI; TRIO HENDARWIN; FAHRRUROZI FAHRRUROZI; ALINA AKHDIYA; DEDEN SAPRUDIN; YULIN LESTARI
Biodiversitas Journal of Biological Diversity Vol. 10 No. 3 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100302

Abstract

Streptomyces spp 234P-16 producing xylanase was isolated from soil sample from Padang, West Sumatra, Indonesia. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase have an optimum condition at pH 5 and 90oC. Crude xylanase have half life time of 4 hours, whereas purified xylanase have half life time of 2.5 hours at 90oC. The molecular mass of purified xylanase was determined to be 42.4 kDa. The Arabinofuranosidase have a Km and Vmax value of 1.98 mg/mL and 523 μmol/minute/mg, respectivelyKey words: xylanase, xylooligosaccharide, arabinofuranosidase, Streptomyces.
Lead (Pb) toxicity effect on physio-anatomy of bead-tree, jatropha, castor bean and Philippine-tung grown in water culture Hamim Hamim; Hanifatunisa; Hadisunarso; Luluk Setyaningsih; Deden Saprudin
Biodiversitas Journal of Biological Diversity Vol. 20 No. 12 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201231

Abstract

Abstract. Hamim, Hanifatunisa, Hadisunarso, Setyaningsih L, Saprudin D. 2019. Lead (Pb) toxicity effect on physio-anatomy of bead-tree, jatropha, castor bean and Philippine-tung grown in water culture. Biodiversitas 20: 3690-3697. Heavy metal contamination in both land and water has been intensively studied because of their broad impact for the environment. Bead-tree (Melia azedarach), Jatropha (Jatropha curcas), castor bean (Ricinus communis) and Philippine tung (Reutealis trisperma) are kinds of non-edible oil-producing species, that are able to grow well on degraded lands and may have potential use for phytoremediation of heavy metals contaminated areas. This study aimed to analyze the response of bead-tree (Melia azedarach), jatropha (Jatropha curcas), castor bean (Ricinus communis) and Philippine-tung (Reutealis trisperma) to lead (Pb) contaminant in water culture experiment based on morphological, physiological and anatomical parameters. Two months old plants were transferred to the media containing Hoagland solution. After three weeks of planting, Pb (NO3)2 treatments with five concentrations, i.e. control (0 mM), 0.5 mM, 1 mM, 2 mM, and 3 mM were added together with Hoagland replacement and the plants were treated for three weeks. The growth, anatomical and physiological parameters were observed during the treatment until three weeks. The results showed that Pb treatment, especially with higher concentration, dramatically decreased plant growth, such as plant height, number of leaves, as well as shoot and root dry weight of all the species. Lead treatment triggered the emergence of free radicals and oxidative stress as indicated by a significant increase of malondialdehyde (MDA) content, while it decreased chlorophyll content of all the species. The higher concentration of Pb (NO3)2 caused the thickness of upper epidermis, lower epidermis, and spongy mesophyll tissue to decrease significantly which contributed to the decrease of leaves thickness. Among the species, Philippine-tung was the most tolerant of Pb toxicity. This species is potential to be used in phytoremediation program of lead-contaminated land such as gold mine area as well as heavy industrial areas.
Co-Authors Afifi, Muhammad Ridho Ahmad Fadli Akhmad Aghzath, Ananjaya Alina Akhdiya ALINA AKHDIYA Amun Amri Anja Meryandini Annas, Iswatun Arrahman, Naufal Budi Riza Putra Citra Ajeng Palupi Dewi Murniati Dian Siska RF Dyah Iswantini Esty Octiana Sari Eti Rohaeti EVIE LESTARIANA Fahrizal Hazra FAHRRUROZI FAHRRUROZI Fendy Firmansyah, Shaeful Hadisunarso Hadisunarso Hadisunarso Hamim Hamim Hanifatunisa Heru Supriyatno Hikmah, Anisa Nurul Hirmas Fuady Putra Ilfa Nuraisyah Imelya Ramanis Irmanida Batubara Ismawati, Iis Kevin Setiawan Latifah Kosim Darusman Luluk Setyaningsih Luluk Setyaningsih Lutfiani Lutfiani Mohammad Khotib Muhammad Farid Nita Trisnawati Novik Nurhidayat Novik Nurhidayat Obie Farobie Permana, Ifri Surya Prima Agung Prihandono Putri Nur Azizah Hutagalung Ramanis, Imelya Rani Apriani Sarah Yasir Shaeful Firmansyah Sudradjat Sudradjat Sudradjat Sudradjat Sukoco Sukoco Tetty Kemala Trio Hendarwin TRIO HENDARWIN TRIO HENDARWIN Umar, Ali Utami Dyah Syafitri Wiwik Hartatik Yohana C Sulistyaningsih YULIN LESTARI