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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia BULETIN OSEANOGRAFI MARINA Journal of Marine Research BIOTROPIA - The Southeast Asian Journal of Tropical Biology Prosiding Seminar Biologi Jurnal Kelautan Tropis Jurnal Mikologi Indonesia Best Journal (Biology Education, Sains and Technology) Jurnal Pasopati : Pengabdian Masyarakat dan Inovasi Pengembangan Teknologi

Mada Triandala Sibero

Departemen Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro

Author-ID : 2514792

Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Education Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Public Health Veterinary

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Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum β-Lactamase (ESBL) E. coli Mada Triandala Sibero; Aninditia Sabdaningsih; Ocky Karna Radjasa; Agus Sabdono; Agus Trianto; Subagiyo Subagiyo
Jurnal Kelautan Tropis Vol 22, No 1 (2019): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

The Trichoderma asperellum MT02 has been reported to has antibacterial activity against the Extended Spectrum β-Lactamase (ESBL) E. coli based on the screening results through an agar plug method. This study aimed to evaluate the antibacterial activity of T. asperellum MT02 and characterize the composition of the bioactive compounds group possessed in its crude extract. The isolate was cultured in the Malt Extract Broth (MEB) media (static, 27 oC, 15 days). The intracellular metabolites from mycelium were extracted using methanol while extracellular metabolites from broth media were extracted using ethyl acetate. The antibacterial activity of crude extracts was tested using the paper disc diffusion method while bioactive compounds were characterized using the phytochemical method. The results showed that the antibacterial activity of the broth media extract performed a greater activity than the crude extract from the mycelium. The crude extract from mycelia only contained flavonoid and phenol hydroquinone compounds while the crude extract from broth media contains alkaloids, flavonoids, phenols hydroquinone and saponins. Kapang Trichoderma asperellum MT02 telah dilaporkan memiliki potensi sebagai penghasil senyawa antibakteri melawan Extended Spectrum β-Lactamase (ESBL) E. coli berdasarkan hasil penapisan melalui metode agar plug. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak kasar kapang T. asperellum MT02 serta komposisi golongan senyawa bioaktif yang dimiliki. Kapang dikultur pada media Malt Extract Broth (MEB) (statis, 27 oC, 15 hari) di mana metabolit intraseluler dari miselium diekstrak menggunakan metanol sedangkan metabolit ekstraseluler dari media kaldu diekstrak menggunakan etil asetat. Aktivitas antibakteri ekstrak kasar diuji menggunakan metode difusi kertas cakram sedangkan senyawa bioaktif dikarakterisasi menggunakan metode fitokimia. Hasil penelitian menunjukkan bahwa aktivitas antibakteri asal ekstrak media kaldu lebih baik dibandingkan ekstrak kasar asal miselium kapang. Ekstrak kasar kapang asal miselia hanya mengandung senyawa golongan flavonoid dan fenol hidrokuinon sedangkan ekstrak kasar asal media kaldu mengandung alkaloid, flavonoid, fenol hidroquinon dan saponin.

Ascidians Outbreak: A Threat For Coral Reefs in Panjang Island, Jepara Dwi Haryanti; Diah Permata Wijayanti; Bambang Yulianto; Mada Triandala Sibero; Lutfillah Arief Ghinaa Shabrina
Jurnal Kelautan Tropis Vol 24, No 3 (2021): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v24i3.13140

Eutrophication and sedimentation have become a major threat to coral reefs in nearby areas with anthropogenic activities. These threats are often accompanied by shifting ecosystems from coral-rich to fast-growing algae-dominated water, and high prevalence of coral disease. In Panjang Island, Jepara, we observed the outbreak of photosynthetic ascidians along with a high sedimentation at the eastern part of the island. The ascidians were seen overgrowing most substrates including corals, macroalgae, dead-coral-algae, and rubbles in April to May 2019. In July and August 2019, observation and data collection using quadrant transect were conducted to monitor the outbreak. The result showed that ascidians were still present in the area, despite becoming pale and smaller. This report shows that the outbreak of these photosynthetic ascidians was not persistent, however, the effect on coral reef health should not be overlooked.

Identifikasi dan Analisis Filogenetik Portunus trituberculatus Dari Perairan Cirebon Menggunakan Barkode Gen COI Mitokondrial Subagiyo Subagiyo; Retna Handayani; Rahayu Rahayu; Triandala Mada Sibero
Jurnal Kelautan Tropis Vol 21, No 2 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Phylogenetic Identification and Analysis Portunus trituberculatus from Cirebon Coast Using the COI Barcode Mitochondrial Portunus trituberculatus spesimen from Cirebon coast were successfully identified using mitochondrial DNA cytochrome c oxydase subunit I (COI) genes. Analysis of haplotype distribution of P. trituberculatus along with the same species from China, Korea, India and the Philippines obtained from NCBI gene banks resulted 17 haplotypes from 25 specimens. Haploid diversity was 0.943 + 0.031 and nucleotide diversity was 0.04821 + 0.0139. The Cirebon specimen is in separated haplotipe from the others. The results of phylogenetic analysis showed that the 25 specimens were clustered into 3 clusters in 2 different lineages with percentages genetic distance were 12.76%, 14.24% and 14.33% respectively. The genetic distance within each cluster ranges from 0 - 2.92%. The Cirebon crab specimen is in the same cluster as the Philippine specimen with 1% genetic distance. Spesimen rajungan Portunus trituberculatus dari perairan Cirebon berhasil diidentifikasi menggunakan gen mitochondrial DNA cytochrome c oxidase subunit I (COI). Analisis distribusi haplotipe dengan data P trituberculatus yang berasal dari China, Korea, India dan Filipina yang diperoleh dari data genebank NCBI didapatkan 17 haplotipe dari 25 spesimen, dengan keragaman haploid 0,943 +0.031 dan keragaman nukleotida 0,04821+0.0139. Spesimen Cirebon merupakan haplotipe yang terpisah dari yang lainnya. Hasil kajian filogenetik menunjukkan 25 spesimen mengelompok ke dalam 3 kluster dari 2 garis keturunan yang berbeda dengan jarak genetik berturut turut 12,76 %, 14,24 % dan 14,33 %. Jarak genetik di dalam masing-masing kluster berkisar antara 0 – 2,92 %. Spesimen rajungan Cirebon berada pada garis keturunan dan kluster yang sama dengan spesimen Filipina dengan jarak genetik 1%.

Kandungan Senyawa Bioaktif dan Aktivitas Biologis Ekstrak Daun Rhizophora apiculata Asal Perairan Teluk Awur, Jepara Mirsa Septiana Mutik; Mada Triandala Sibero; Widianingsih Widianingsih; Subagiyo Subagiyo; Rudhi Pribadi; Dwi Haryanti; Ambariyanto Ambariyanto; Retno Murwani
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.14287

Rhizophora apiculata is a type of mangrove that has the ability to adapt to extreme environmental conditions such as temperature, low oxygen levels, and high salinity. This adaptability affects the production of secondary metabolites. Information about the antibacterial activity of this mangrove against MDR (Multi-Drug Resistant) bacteria is still very limited. The content of secondary metabolites produced by mangrove R. apiculata is also expected to affect antioxidant activity against free radicals. The purposes of this study were to examine the presence of bioactive compounds by phytochemical tests and to evaluate the antibacterial activity against MDR bacteria such as Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, and Bacillus subtilis; and antioxidants property of R. Apiculata leaves that were collected from Teluk Awur, Jepara. The leaves were extracted using the multilevel maceration method with solvent sequence n-hexane, ethyl acetate and methanol. Metabolite finger printing using TLC method was carried out to detect the presence of alkaloids, flavonoids, saponins, phenolics, quinones, steroids, and triterpenoids. The antibacterial test was carried out using agar well diffusion method while the antioxidant test was carried out using the DPPH method. The results of the phytochemical test showed that there were groups of alkaloids and steroids in the n-hexane solvent; alkaloids, phenolics, and steroids in ethyl acetate solvent; as well as alkaloids, flavonoids, phenolics, and saponins in methanol solvents. The results of this study indicate that R. apiculata from Teluk Awur Coastal Waters, Jepara had no potential as an antibacterial against MDR (Multi-Drug Resistant) bacteria, however the methanol extract has the potential to be used as an antioxidant with an IC50 value of 85.999 ppm. The bioautography showed that compounds from the phenol group, flavonoids, triterpenoids and b-carotene pigments acted as antioxidant agents in the leaf extract of R. apiculata. Rhizophora apiculata merupakan salah satu jenis mangrove yang memiliki kemampuan beradaptasi terhadap kondisi lingkungan ekstrem seperti suhu, kadar oksigen rendah dan salinitas tinggi. Kemampuan beradaptasi tersebut mempengaruhi produksi matabolit sekunder. Informasi mengenai kemampuan aktivitas antibakteri mangrove jenis ini melawan bakteri MDR (Multi Drug Resistent) masih sangat terbatas. Kandungan metabolit sekunder yang dihasilkan mangrove R. apiculata ini juga diharapkan dapat mempengaruhi aktivitas antioksidan melawan radikal bebas. Tujuan dari penelitian ini adalah untuk mengkaji kandungan senyawa bioaktif serta aktivitas biologis berupa antibakteri melawan bakteri MDR Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus dan Bacillus subtilis; dan antioksidan dari ekstrak daun mangrove R. apiculata asal perairan Teluk Awur, Jepara. Sampel diekstraksi menggunakan 3 pelarut berbeda (n-heksana, etil asetat dan metanol) dengan metode maserasi bertingkat. Analisis metabolit sidik jari dilakukan menggunakan plat KLT untuk mengetahui kandungan senyawa alkaloid, flavonoid, saponin, fenolik, kuinon, steroid dan triterpenoid. Uji antibakteri dilakukan menggunakan metode sumuran sedangkan uji antioksidan dilakukan menggunakan metode DPPH. Hasil uji fitokimia menunjukkan terdapat golongan senyawa alkaloid dan steroid pada pelarut n-heksana; alkaloid, fenolik dan steroid pada pelarut etil asetat; serta alkaloid, flavonoid, fenolik dan saponin pada pelarut metanol. Hasil penelitian ini mengindikasikan bahwa R. apiculata asal perairan Teluk Awur, Jepara tidak potensial sebagai antibakteri melawan bakteri MDR (Multi Drug Resistent), akan tetapi ekstrak metanol potensial digunakan sebagai antioksidan dengan nilai IC50 85,999 ppm. Tahapan bioautografi menunjukkan bahwa senyawa dari golongan fenol, flavonoid, triterpenoid dan pigmen b-karoten berperan sebagai agen antioksidan pada ekstrak daun R. apiculata.

Co-Authors Agus Sabdono Agus Sabdono Agus Trianto Agus Trianto Ambariyanto Ambariyanto Aninditia Sabdaningsih Antonius Budi Susanto Bambang Yulianto Diah Permata Wijayanti Dwi Haryanti Ivan Permana Putra Kustiariyah Tarman Lutfi Hanafi Lutfillah Arief Ghinaa Shabrina Mirsa Septiana Mutik Nirwani Soenardjo Nunung Febriany Sitepu Nur Syafiqoh Nur Taufiq-Spj Ocky Karna Radjasa Ocky Karna Radjasa Pola Risda Aswita Silitonga Prasetyana Ajeng Refamurty Rahayu Rahayu Retna Handayani Retno Murwani Retno Murwani Rita Sahara Rudhi Pribadi Sari Poncowati Siti Nur Kholishah Stefanie Jessica Henny Larasati Subagiyo Subagiyo Tao Zhou Tiara Ulfa Bachtiarini Wahyu Aji Mahardhika Widianingsih Widianingsih Wilis Ari Setyati Yasuhiro Igarashi Yolanda Christia Simanjuntak

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