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PENGARUH PEMBERIAN AIR KUNYIT (CURCUMA DOMESTICA) TERHADAP PROFIL DARAH MERAH (JUMLAH ERITROSIT, HEMOGLOBLIN DAN HEMATOKRIT) PADA AYAM BROILER (Effect Giving Water Of Turmeric (Curcuma Domestica) On Cell Red Blood Profiles (Total Of Erythrocytes, Hemoglobin And Hematocrit) Of Broiler Chickens) Hanifa, Khoirina; Murwani, Retno; Isroli, Isroli
Jurnal Pengembangan Penyuluhan Pertanian Vol 14, No 2 (2017): Jurnal Pengembangan Penyuluhan Peternakan
Publisher : UPPM Politekik Pembangunan Pertanian Yogyakarta Magelang (Polbangtan Yoma)

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian air kunyit terhadap profil darah merah (jumlah eritrosit, kadar hemoglobin, dan hematoktrit) pada ayam broiler. Materi yang digunakan yaitu 200 ekor ayam broiler (DOC/day old chicks) strain Lohman 202 (PT Japfa Comfeed Indonesia Tbk) dengan bobot badan awal rata-rata 41.48±0.99 gram. Pemberian air minum pada minggu pertama dan pada hari ke-11 hingga selesai menggunakan air kunyit. Rebusan air kunyit disiapkan dengan merebus air mineral 600 ml hingga mendidih, kemudian 10 gram kunyit yang telah dicuci bersih dan dimemarkan,dimasukan dalam rebusan air mendidih selama ± 10 menit. Pemberian air minum dilakukan secara ad libitum. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan dan setiap unit percobaan terdiri dari 8 ekor ayam Adapun perlakuan yang diberikan yaitu 100% air minum, 75% air minum : 25% air kunyit, 50% air kunyit : 50% air minum, 75% air kunyit : 25% air minum, dan 100% air kunyit. Hasil penelitian menujukkan bahwa tidak ada pengaruh pemberian air kunyit terhadap profil darah merah ayam broiler. Simpulan dari penelitian yaitu pemberian air kunyit dalam air minum tidak mengubah profil darah dan tetap berada dalam kisarannormal. Kata Kunci: kunyit, broiler, eritosit, hemoglobin, hematokrit. 
STATUS DARAH DAN TITER NEWCASTLE DISEASE PADA BURUNG PUYUH PETELUR YANG DIBERI RANSUM MENGGUNAKAN TEPUNG DAUN OROK-OROK (Crotalaria usaramoensis) SEBAGAI SUMBER PROTEIN Ariyani, Siti Anisah; Wahyono, Fajar; Murwani, Retno
Animal Agriculture Journal Vol 1, No 1 (2012): Volume 1, Nomor 1, Tahun 2012
Publisher : Animal Agriculture Journal

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Abstract

The experiment was carried out to study of adding orok-orok (Crotalariausaramoensis) leaf meal in quail diets with different level on hemoglobin, PVC,and ND titer of layer quail. The data were analyzed by a Completely RandomizedDesign and continued with Duncan’s Multiple Range Test for differences. Theexperiment used 100 quail 7 weeks old which were randomly devided into 4groups and each group had repeated 5 times and each consisted of 5 quails. Thegroups were T0 (basal diet as a control), T1 (basal diet with 3% orok-orok leafmeal), T2 (basal diet with 6% orok-orok leaf meal), dan T3 (basal diet with 9%orok-orok leaf meal). Diets and water were offered ad libitum. Data werecollected during 35 days to obtain the data of total amount of protein intake,hemoglobin, and ND titer. PVC showed that there was no significant different(P>0,05). Conclusion of this research, orok-orok leaf meal can used quail layersdiet until 3%.Key Words: Orok-Orok Leaf Meal, Hemoglobin, Hematokrit, ND Titer.
Kandungan beta karoten, polifenol total dan aktifitas ”merantas” radikal bebas kefir susu kacang hijau (Vigna radiata) oleh pengaruh jumlah starter (Lactobacillus bulgaricus dan Candida kefir) dan konsentrasi glukosa Supriyono, Teguh; Murwani, Retno; Nurrahman, Nurrahman
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 2, No 2 (2014)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (427.901 KB) | DOI: 10.14710/jgi.2.2.66-72

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Background: Kefir is a well known fermented dairy milk product which is functional for gut health. However kefir can also be made from vegetable milk such as soy or mungbean milk. Objective: To study the chemical  and functional property of Mungbean milk (MBM) Kefir Methods:Mungbean milk was fermented into MBM Kefir using Lactobacillus bulgaricus and Candida kefir. Three levels of cultures and glucose concentration were tested and a complete randomized design experiment was employed. The resulting MBM Kefir were tested for β-carotene level, total polyphenol content, and free radical scavenging activity. Beta carotene level, total polyphenols and free radicals scavenging activity were determined by spectrophotometry, Folin-Ciocalteu method, and DPPH radical scavenging assay respectively. The data were analyzed by ANOVA and when the result of ANOVA was significant, Tukey’s multiple comparison was conducted. Results: Culture concentration increased  β-carotene level (p = 0.049), total polyphenol (p = 0.015) and free radical scavenging activity (p = 0,000) of MBM kefir. Glucose concentration increased total polyphenol (p = 0.002), but had no effect on free radical scavenging activity and β-carotene level. However, there were no interaction among treatments at 0.05 significance level. Total acid level ranged from 1.57 to 2.54%, with the highest and level at 15% cultures concentration and 15% glucose concentration. Alcohol level ranged from 0.26 to 0.90% which was in accordance with the maximum standard for kefir (1%). Conclusion: An optimal product of mungbean milk kefir with highest free radical scavenging activity can be obtained using 15% cultures (Lactobacillus bulgaricus and Candida kefir) and 10% glucose concentration.
Pengaruh Pemberian Serbuk Buah Semu Jambu Monyet (Annacardiumaccidentale L) Sebagai Aditif Pakan Terhadap Profil Serum Lemak Dan Glukosa Broiler Retno Murwani dan Siti Susanti, Adella. C. Maharani,
AGROMEDIA: Berkala Ilmiah Ilmu-ilmu Pertanian Volume 37 No. 1 Maret 2019
Publisher : Sekolah Tinggi Ilmu Pertanian Farming Semarang

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Abstract

ABSTRAKPenelitian ini bertujuan mengetahui pengaruh pemberian serbuk Buah Semu Jambu Monyet (BSJM) dalam aditif pakan terhadap profil serum lemak dan glukosa broiler yang memiliki arti penting pada kualitas karkas broiler antara lain kadar kolesterol darah, HDL, LDL, trigliserida dan glukosa. Penelitian dilaksanakan pada tanggal 14 Januari – 17 Febuari 2015 di kandang ayam, peternakan rakyat Ds.Kaliancar, Podorejo, Ngaliyan, Semarang dan Balai Laboratorium Kesehatan, Semarang. Materi yang digunakan adalah Day Old Chick (DOC) ayam broiler strain CP-707 sebanyak 140 ekor (unsexed) dengan bobot badan 40,00 g± 0,84.  Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL).  Perlakuan yang diuji adalah dosis serbuk BSJM terdiri atas T0 (tanpa penambahan), T1 (penambahan 0,25% serbuk BSJM), T2 (penambahan 0,5% serbukBSJM) dan T3 (penambahan 1,00% serbuk BSJM). Hasil penelitian menunjukan ada pengaruh nyata (P<0,05) terhadap kadar kolesterol darah, HDL, LDL dan trigliserida, tetapi tidak berpengaruh nyata (P>0,05)  terhadap kadar glukosa pada pemberian serbuk buah semu jambu monyet (Annacardium accidentale L) pada broiler. Kata kunci: serbuk BSJM, profil serum lemak dan glukosa, broiler
PENGARUH PEMBERIAN LARUTAN GULA KELAPA DAN JUS UMBI BIT TERHADAP BOBOT ORGAN USUS HALUS, PROVENTRIKULUS DAN VENTRIKULUS PADA ANAK AYAM BROILER Zitni Ilma; Retno Murwani; Rina Muryani
JURNAL LITBANG PROVINSI JAWA TENGAH Vol 14 No 2 (2016): Jurnal Litbang Provinsi Jawa Tengah
Publisher : Badan Perencanaan Pembangunan, Penelitian dan Pengembangan Daerah Provinsi Jawa Tengah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36762/jurnaljateng.v14i2.388

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The aim of this study was to determine the effect of palm sugar and beet juice in drinking water on relative weight of small intestine, proventriculus, and ventriculus of broiler chickens. One hundred and ninety five unsexed day old chicks (DOC) strain cobb were completely randomized into 5 treatments: T0 (control), T1 (1% palm sugar solution), T2 (1% beet root juice), T3 (2% palm sugar solution), and T4 (2% beet root juice). Palm sugar solution and beet root juice were given as drinking water. Chick from each treatment was taken on 2th, 3th, and 4th day. The chicks were sacrificed then dissected, small intestine, proventriculus, and ventriculus were taken and weighed. The results showed that 1 % and 2% palm sugar and beet juice in dringking water increased relative weight of small intestine at 4th day.
PENGARUH PENGGUNAAN TAWAS PADA PAKAN TERHADAP TOKSISITAS DAN KADAR ALUMINIUM ORGAN TIKUS IN VIVO Nurrahman -; Retno Murwani; Nur Yazid
Jurnal Pangan dan Gizi Vol 3, No 2 (2012): Kajian Pangan dan Gizi
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jpg.3.2.2012.%p

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The use of tawas as an food additive has widely used in processing, such as the manufacture of smoked fish, salted eggs and meatballs. This study aims to determine the effect of using tawas as addition in food processing to health. There are two groups of mice each containing 11 mice, one group was givena diet that has been given 0.8 percent tawas and the other one was given diet without the addition of tawas (control group). The issue of diet and water is by ad libitum conducted in 30 days. During the experiment it was recorded the feed intake, performance (behavioral), the appearance of the skin, eyes,feces and urine, and body weight. After the maintenance mice were killed, then conducted an analysis of organ weight and levels of aluminum organ. There was a tendency of tawas addition on feed causing less consumption and weight loss on mice. Granting tawas to treated rats showed no differences inphysical appearance of behavior, skin, eyes and feces, and also no differences in organ weight. While there are differences appear in the urine, which treated rats urine looks a bit turbid than the other one.There is deposits of aluminum in organs like heart, liver, lung, pancreas, kidney and bone. Deposit aluminum at pancreas is the most than the other organs. There are not found any clinical symptoms in both control and treated rats. Key words= Alum, toxicity, aluminium
INSECT CELLS AS HOSTS FOR RECOMBINAT PROTEINS Retno Murwani
JOURNAL OF COASTAL DEVELOPMENT Vol 1, No 1 (1997): Volume 1, Number 1, Year 1997
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Since the development of recombinant baculovirus expression system, insect cell culture has rapidly gain popularity as the method of choice for production of a variety of biologically active proteins. Up to date tens of recombinant protein have been produced by this method commercially or non-commercially and have been widely used for research. This review describes the basic concept of baculovirus expression vector and the use of insect cells as host for recombinant proteins. Examples of the recombinant proteins produced by this system are given.
Skrining Bahan Anti Kanker pada Berbagai Jenis Sponge dan Gorgonian Terhadap L1210 Cell Line Agus Trianto; Ambariyanto Ambariyanto; Retno Murwani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 3 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.778 KB) | DOI: 10.14710/ik.ijms.9.3.120-124

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Sejarah evolusi yang panjang pada biota laut menyebabkan biota laut mempunyai keanekaragaman molekul yang sangat tinggi. Potensi biota laut tersebut sebagai sumber obat anti kanker menjadi objek penelitianpenting dalam tahun-tahun terakhir. Bahan yang dipergunakan dalam penelitian ini adalah sponge dan gorgonian yang dikoleksi dari perairan Jepara pada kedalaman 1-3 m dan di perairan Labuhan Bajo, Florespada kedalaman 3-38 m. Sampling dilakukan dengan Skin diving dan SCUBA diving. Sampel kemudian diekstrak dengan metanol. Selanjutnya ekstrak diujikan terhadap sel kanker leukemia (L-1210 cell line) dengankonsentrasi 0, 1, 5 dan 10 ppm. Uji dilakukan pada media RPMI lengkap dan penghitungan daya hambat dilakukan dengan metoda direct counting. Ekstrak yang diperoleh dari sponge dan gorgonian berkisar antara 0,55 – 24,7% dari berat kering atau 0,36-7,34% dari berat basahnya. Seluruh ekstrak dari sampel-sampel mampu menghambat pertumbuhan L1210 cell line dan layak untuk pemurnian lanjut. Ekstrak metanol tiga jenis sponge (Xestospongia sp2 dan Phyllospongia sp1 dan UP8) dan fraksi etil asetat dari ekstrak gorgonian I. hippuris mempunyai IC-50 < 3 mg/mL, ekstrak metanol dari delapan jenis sponge (Agelas nakamurai, Ircina ramosa, A06, Phyllospongia lamellosa , Phyllospongia sp, UP9, Calispongia sp dan Fascaplynopsis sp) mempunyai IC-50 < 5 mg/mL, dan ekstrak metanol dari lima jenis sponge (Hyrtios erecta, Xestospongia sp, Cladocroce sp, Oceanapia cf. Amboiensis dan Haliclona sp.) dan fraksi air dari ekstrak gorgonian I. Hippuris mempunyai IC-50 < 10 mg mL Satu sponge Xestospongia sp 1 mengandung ekstrak yang mempunyai IC - 50 > 10 mg/mL.Kata kunci : Sponge, Gorgonian, L-1210, sel kanker, IC-50The long history of the evolution of marine organisms has made these organisms as a source of high molecular diversity. In search of a new anti cancer drugs, these organisms has become an intense object of research.Sponges and the gorgonian were colllected from Jepara and Labuan Bajo-Flores water on a depth of 1-3 m and 3-38 m repectively by Skin and SCUBA diving. The samples were extracted with methanol. Theextract were assayed against cancer cell (L-1210 cell line) with tested concentration range of 0, 1, 5 and 10 ppm. The cell line was cultured in complete RPMI media and growth inhibition was measured by directcounting. Methanol extract of sponges and gorgonian yielded approximately 0.55 – 24.7 % dry weight or 0,36 – 7.34 % wet basis. All methanol extract of the samples could inhibit the growth of L1210 cell line and is potential for further purification. Methanol extract from 3 species of sponges (Xestospongia sp2 dan Phyllospongia sp1 and UP8) and one ethyl acetate fraction of I. hippuris had IC-50 value < 3 mg/mL. Methanol extract of 8 species of sponges (Agelas nakamurai, Ircina ramosa, A06, Phyllospongia lamellosa, Phyllospongia sp, UP9, Calispongia sp dan Fascaplynopsis sp) had IC-50 < 5 mg/mL, methanol extract of 5 species of sponges (Hyrtios erecta, Xestospongia sp, Cladocroce sp, Oceanapia cf. Amboiensis and Haliclona sp.) and one water extract of I. hippuris had IC-50 < 10 mg mL. Methanol extract of one species of sponge (Xestospongia sp 1) had IC-50 > 10 mg/mL.Key words : Sponge, Gorgonian, L-1210, Cancer cell, IC-50
Uji Toksisitas Ekstrak Gorgonian Isis hippuris Terhadap Nauplius Artemia salina Agus Trianto; Yan Yan HAS; Ambariyanto Ambariyanto; Retno Murwani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 2 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.962 KB) | DOI: 10.14710/ik.ijms.9.2.61-66

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Beberapa jenis organisme laut merupakan sumber alam yang potensial untuk bahan obat. Salah satu sumber daya laut yang cukup potensial untuk dapat dimanfaatkan adalah gorgonian Isis hippuris. Hewan ini hidup di ekosistem terumbu karang tersebar di perairan dangkal dan jernih terutama di pertengahan dasar karang. Tujuan penelitian ini adalah untuk mengetahui aktivitas toksisitas ekstrak gorgonian Isis hippuris terhadap nauplius Artemia salina, mengetahui LC50 -24 jam fraksi toksik dan mengidentifikasi senyawa yang berperan dalam toksisitas. Penelitian dilaksanakan di laboratorium Ekplorasi dan Bioteknologi Ilmu Kelautan UNDIP, Teluk Awur Jepara. Hasil penelitian menunjukan perlakuan ekstrak gorgonian I. hippuris fraksi etil asetat terhadap nauplius A. salina dari 12 fraksi KKT diperoleh 5 fraksi KKT yang toksik. Berdasarkan analisa probit diketahui fraksi KKT 9 memiliki aktivitas toksik terbaik dengan nilai LC 50-24 jam sebesar 16,98 ppm. Hasil identifikasi dengan GC/MS diperoleh golongan senyawa-senyawa Hidrokarbon dan Asam lemak yaituNaphthalene, Xylane, Phenylacetonitrile, 1,2 Benzenedicarboxylic dan senyawa turunan phenol.Kata kunci: Isis hippuris, Artemia salina, toksisitas, BSLT.Several marine organisms are known to have bioactive substances which are very potential for drugs materials. One of this organism is gorgonian, Isis hippuris which live in coral reef ecosystems. The objective of this study is to investigate I. hippuris extract toxicity to nauplius of brine shrimp Artemia salina. This was done by calculating LC50-24 h and followed by identifying its compound. This study was done at Exploration and Biotechnology Laboratory, Marine Science department. UNDIP, Awur Bay, Jepara. The results showed that from 12 ethyl acetate fraction open column chromatography, 5 of them were toxic. Based on Probit analysisit was found that fraction 9 was the best fraction which gave LC50-24 h 16,98 ppm. Based on GC/MS results there are several compound found i.e. hydrocarbon, lipid acid (napthalene, xylane, phenylacetonitrile, 1,2benzenedicarxulic) and phenol derived compound.Key words : Isis hippuris , Artemia salina, toxicity, BSLT
Acceptability and Nutrient Content of Wet Noodles Fortified with Inorganic or Organic Iron and Provitamin A from Pumpkin (Cucurbita moschata) Ninik Rustanti; Retno Murwani; Syaiful Anwar
MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 3 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACTBackground: Iron fortification of wet noodles is a choice among many others, to reduce iron deficiency anemia. Organic andinorganic iron interact with provitamin A. An experiment was conducted to fortify wet noodles with organic and inorganic iron and provitamin A from pumpkin, and to study its effect on acceptance and its iron and provitamin A content.Methods: Six groups were used in this experiment to test optimal level of iron fortification (100 ppm, 150 ppm, 200 ppm of FeSO4 or organic iron). As for pumpkin, 10%, 15% and 20% were used. To test iron and provitamin A content, 5 groups were employed: wet noodle alone, wet noodle + (FeSO4), + (organic Fe), + (FeSO4 & pumpkin), and + (organic Fe & pumpkin) respectively. Data were evaluated by one-way ANOVA and continued by Duncan’s test with 95% CI.Result: Noodle fortified with 200 ppm iron was acceptable in term color, taste and texture. Adding 15% pumpkin improved texture optimally compared to 10% and 20%. In term color and taste, 10%, 15% and 20% pumpkin addition gave similar acceptance. Fortification of iron and provitamin A from pumpkin didn’t affect the moisture, protein, fat, fiber, and carbohydrate content but increased iron, ash and betacarotene content. Highest content of iron and provitamin A were found in noodles + (FeSO4 + pumpkin) and noodles + (organic Fe + pumpkin).Conclusion: Iron and provitamin A fortified wet noodles can serve as a choice for eliminating iron deficiency anemia in Indonesia. Further studies to see the bioavailability and effectiveness of fortified wet noodles to reduce anemia are needed.Keywords: Fortification, organic iron, inorganic iron, provitamin A pumpkin, wet noodlesABSTRAKDaya terima dan kandungan gizi mie basah yang difortifikasi zat besi anorganik dan organik serta provitamin A labu kuning (Cucurbita moschata)Latar belakang: Fortifikasi mie basah merupakan salah satu pilihan untuk menanggulangi permasalahan anemia gizi besi. Besi organik dan anorganik dapat berinteraksi dengan provitamin A. Penelitian ini bertujuan untuk memfortifikasi mie basah dengan besi organik dan anorganik serta provitamin A labu kuning, dan untuk mengetahui pengaruhnya terhadap daya terima, kadar besi dan provitamin A pada mie basah.Metode: Enam perlakuan digunakan dalam penelitian untuk optimasi kadar besi fortifikan (100 ppm, 150 ppm, dan 200 ppm FeSO4 atau Fe organik). Labu kuning yang digunakan 10%, 15%, dan 20%. Untuk mengetahui kadar besi dan provitamin A digunakan 5 perlakuan: mie basah, mie basah + (FeSO4), + (Fe organik), + (FeSO4 & labu kuning), dan + (Fe organik & labu kuning). Data dianalisis dengan one-way ANOVA dan dilanjutkan dengan uji Duncan dengan 95% CI.Hasil: Mie yang difortifikasi dengan 200 ppm zat besi dapat diterima dari sisi warna, rasa dan tekstur. Penambahan labu kuning 15% memberikan tekstur yang lebih baik dibandingkan 10% dan 20%. Dari sisi warna dan rasa, penambahan labu kuning 10%, 15% dan 20% mempunyai daya terima yang sama. Fortifikasi besi dan provitamin A labu kuning tidak mempengaruhi kadar air, protein, lemak, serat dan karbohidrat tetapi meningkatkan kadar besi, abu dan betakaroten. Kadar besi dan provitamin A tertinggi terdapat pada mie + (FeSO4 +labu) dan mie + (Fe organik + labu).Simpulan: Mie basah yang difortifikasi besi dan provitamin Aberupa labu kuning dapat menjadi alternatif untuk mengatasianemia gizi besi di Indonesia. Diperlukan penelitian lanjutanuntuk mengetahui bioavailabilitas dan keefektifan mie yangdifortifkasi untuk mengurangi anemia.