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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus

Desak Nyoman Dewi Indira Laksmi

Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB Sudirman, Denpasar, Bali.

Author-ID : 252239

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Paparan Formalin Menghambat Proses Spermatogenesis pada Mencit Luh Gde Sri Surya Heryani; Ni Nyoman Werdi Susari; I Made Kardena; Desak Nyoman Dewi Indira Laksmi
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.505 KB)

Formaldehyde is one of the exogenous free radicals. During formaldehyde exposure, there will be morefree radical or reactive oxygen compound formed through electron transport chain. Excessive reactiveoxygen compound triggers the process of lipid peroxide reaction in the membrane of spermatozoa cell. Theaims of this study were to find out that formaldehyde exposure caused abnormalities in the number of thenumber of spermatogonium type A, spermatogonium type Pachytene, 7th spermatid and 16th spermatid inspermatogenesis process.This study was experimentally and randomly pretested-posttested- with controlgroup design. The samples of this study were adult male mice strain Balb-C (age 2-3 months) with thefollowing criteria: body weight between 22-25 grams and healthy. Randomly, 48 mice were divided intothree groups, were control group, the first treatment group and the second treatment group. Prior to thetreatment, were taken a half from each group for the pre-test, by preparing microscopic preparation testicleand examination was performed to the total spermatogenic cells. The rests of the mice were used as posttestexamination after 35 days treatment. On the 36th day, all the rest mice were necropsied for microscopictesticle preparation. The result of this study showed that the formaldehyde exposure caused significantlydecrease in the number of spermatogenic cells (p<0,05), the average of type A spermatogonium cells atcontrol group, 1st treatment group and 2nd treatment group were 39,90±0,51; 20,42±0,72; 15,65±0,88respectively; spermatogonium type Pachytene were 48,47±1,28; 32,60±3,06; 23,14±3,16 respectively; 7thspermatid were 97,47±5,28; 39,98±4,28; 30,36±2,96 respectively and16th spermatid were 73,08±4,05;21,70±1,70; 16,38±1 respectively.It can be concluded that the formaldehyde exposure decreased the amountof spermatogenic cells. The result of this study is expected to be used as the baseline for further study inorder to measure formaldehyde content as a free radical in testicle .

Pemberian Ekstrak Oncom Hitam dan Merah Memperpanjang Siklus Estrus dan Mempertebal Endometrium Tikus Putih Hanifah Alshofa Nurul Aini; Desak Nyoman Dewi Indira Laksmi; Ni Luh Eka Setiasih; Steven Dwi Purbantoro
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

hytoestrogens are active compounds, derived from plants, which have a similar structure and function as estrogen. Phytoestrogens are commonly found in legumes. Oncom, which is assumed containing phytoestrogens, is one of the most famous legumes food from Indonesia and widely consumed daily in West Java. This study was aimed to determine the effect of oncom extract on estrous cycle, endometrium thickness, and the number of antral follicles in productive age rats (Rattus novergicus). This experimental study was using 21 three-to-four-month-old fertile female rats and divided into three groups. Group I (K) was considered as a control group without any treatment. Group II and III were treatment groups which were given black (H) and red (M) oncom extracts 0.005 g/g body weight, respectively, orally with a feeding tube for 14 days. The length of the estrous cycle was measured by performing vaginal swab with interval 12 hours after first treatment was given and during the treatment. Endometrium thickness and the number of antral follicleswere measured by collecting the organs uterus and ovary for histological purpose with paraffin method after rats were euthanized post-treatment oncom extracts for 14 days. Data were analyzed by ANOVA and continued with LSD test. The total length of estrous cycle of control group, black oncom extract group, andred oncom extract group was 107,43±3,16 hours, 141,43±15,36 hours, and 161,14±17,10 hours, respectively. The mean of endometrium thickness of control group, black oncom extract group, and red oncom extract group was 346,945±65,88 ?m, 485,740±86,69 ?m, and 533,904±78,93 ?m, respectively. The number ofantral follicles of control group, black oncom extract group, and red oncom extract group was 6,00±1,54, 8,43±2,99, and 9,14±2,72, respectively. Results showed that black and red oncom extracts had a significant effect on the length of estrous cycle and endometrium thickness in rats, yet there is no significant difference in the number of antral follicles. In summary, black and red oncom extracts had effects on the length of estrous cycle and endometrium thickness, yet there was no effect on the number of antral follicles.

Viabilitas Spermatozoa Ayam Hutan Hijau dalam Pengencer Posfat Kuning Telur Ditambah Laktosa pada Penyimpanan 5oC (GREEN JUNGGLE FOWL SPERM VIABILITY IN EGG YOLK PHOSFAT DILUENTS CONTAINING LACTOSE STORAGED AT 5OC) Wayan Bebas; Desak Nyoman Dewi Indira Laksmi
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.855 KB)

The objective of this study was to determine the addition of lactose on egg yolk phosphate diluentsstored at 5°C to the plasma membrane intact, acrosome membranes intact of green jungle fowlspermatozoa, fertility and hatchability of chicken eggs. This study used completely randomized designwith three treatment groups. Treatment I (T0): egg yolk phosphate diluents only (control), treatment II(T1): egg yolk phosphate diluents + lactose 0.3%, and treatment III (T2): egg yolk phosphate diluents +lactose 0.6 %. Each group was replicated nine times. Semen was collected from eight green jungle fowl andevaluated both macroscopically and microscopically. Semen was diluted with each treatment with a singleinsemination dose (0.5 mL) with a concentration of 150 million progressively motile cells. Once diluted,semen was stored at 3-5°C for 48 hours and then was observed for the plasma membrane of intact,acrosome membrane of intact, and some semen were inseminated on local female chicken village forfertility, and hatchability eggs observation. The results showed that the addition of lactose 0.3% and 0.6%providing significant increase (P <0.05) on plasma membrane intact, acrosome membrane intact, fertilityand egg hatchability when compared to controls. However the addition of 0.3% and 0.6% lactose did notshowed a significant effect (P> 0.05). This study concluded that the addition of lactose on egg yolk phosphatediluents for storing green jungle fowl spermatozoa at 3-5oC for 48 hours can improve the plasma membraneintact, acrosome membranes intact, fertility and hatchability of chicken eggs.

Hubungan Antara Kadar Leptin saat Partus dengan Kadar Estrogen Estrus Post-partus pada Sapi Bali I Putu Suparman; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi
Buletin Veteriner Udayana Vol. 14 No. 4 August 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Leptin is one of metabolic product that regulates hypothalamus pituitary ovaries. This study aims to determine the relationship of leptin levels during parturition with estrogen levels estrus post-partus in bali cattle. The data source of this research is bali cattle during parturition where the main blood sample is taken less than 24 hours after parturition. Continued sampling of cow's blood when showing symptoms of estrus postpartum. Determination of leptin and estrogen levels was carried out using the Direct Elisa, Double Antibody Sandwich method. Data were analyzed descriptively with the Correlation and Regression statistical tests. The results showed that the average level of leptin hormone at parturition ( ± SD) was 2.60 + 0.47 ng/ml and the average level of post-partus leptin estrus hormone ( ± SD) was 5.42 + 0.47 ng/ml. While the average levels of the hormone estrogen estrus post-partus ( ± SD) is 105.51 + 19.72 pg/ml. This shows an increase in the level of the hormone leptin from parturition to estrus post-partus which has an impact on increasing estrogen levels. By using a linear regression test it was found that leptin hormone levels had a positive effect on estrogen hormone levels. From the results of the study it can be concluded that there is a close relationship between leptin levels during parturition with estrogen levels during estrus post-partus.

Waktu Munculnya Estrus Postpartum pada Berbagai Paritas pada Sapi Bali Muhammad Ihwanul Muslimin; Desak Nyoman Dewi Indira Laksmi; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p06

Parity is a period in the process of animal recycling with an indication of the number of partis of livestock. This research discusses to study the time of postpartum esterus in Balinese cattle with various parities. This research was an observational analytic study with a cross-sectional study design. The analysis used in this research is descriptive analysis. Samples used in this study were 21 cows with various parity added 7 cows with 1 calf, 7 cow with 2 calves and 7 cows with more than 2 calf. The results showed that cows with various parities increased the post parturition estrus delay due to the difficulty in obtaining feed. From these results it can be concluded that there are differences in post partus estrus in various parity I, II and III due to lack of optimal management in maintenance, especially in terms of feed. It should be noted again in the management of maintenance, especially feed to optimize the reproductive function of the animal.

Seks Rasio pada Anak Tikus yang Dilahirkan Akibat Pemberian Bee Pollen Kresensia Cyntia Dosom; Desak Nyoman Dewi Indira Laksmi; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p13

The purpose of this study was to see how effective bee pollen is at determining sex in rats. This was an experimental study with three treatments and control group that used a completely randomized design. There was no bee pollen in control group. Then in P1, only female rats were given bee pollen (18mg), in P2 only male rats (22mg), and in P3 both male and female rats were given the same dose of bee pollen. Male and female rats were mated after receiving bee pollen for 49 days, and the sex ratio of the newborn rats was calculated after 21 days of gestation. ANOVA was used to analyze th data before proceeding with Duncan’s test. The findings of this study revealed that bee pollen could increase the proportion of male rats. This is because giving bee pollen to male rats allows more Y-chromosome spermatozoa to be produced and survive in this condition in the female reproductive tract. Andosperms benefit from this condition because they can move faster and more agilely than gynosperma. More male offspring are produced because the Y sperm (andosperm) or male sperm first reaches the egg and fertilizes it. Giving male and female rats bee pollen at the same time can increase the sex ratio of male rat pups.

Co-Authors Achmad Noerkhaerin Putra AJI WAHYU WICAKSONO Annisya Syarifuddin Budi, Josephine Aurora Dhayanti, Ni Luh Evy EVA AGUSTINA SIAHAAN Hanifah Alshofa Nurul Aini Haq, Nurul Ikhsanul Herdi Wahyu Adi Prananda I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Bagus Trilaksana I Ketut Puja I Made Agus Suryanatha I Made Damriyasa I Made Kardena I Putu Sampurna I Putu Sudana Agustina I Putu Suparman I Wayan Bebas I Wayan Gorda juliantari, Ni Komang Ade Komang Gita Permana Kresensia Cyntia Dosom Kristyari, Ni Putu Gita Luh Gde Sri Surya Heryani MADE KOTA BUDIASA Mahasanti, I Gusti Ayu Puji Muhammad Ihwanul Muslimin Ni Luh Eka Setiasih Ni Nyoman Werdi Susari Pridayanti, Ni Kadek Nila Steven Dwi Purbantoro Tessa Yuli Oktaviami Tjok G. O. Pemayun, Tjok G. O. Tjok Gede Oka Pemayun Yudiani, Putu Mira

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