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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus

Desak Nyoman Dewi Indira Laksmi

Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB Sudirman, Denpasar, Bali.

Author-ID : 252239

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Pemunculan Pubertas Sapi Bali Dara Peliharaan Kelompok Ternak di Wilayah Kerja Pusat Kesehatan Hewan Sobangan, Mengwi, Badung, Bali Pridayanti, Ni Kadek Nila; Laksmi, Desak Nyoman Dewi Indira; Sampurna, I Putu
Indonesia Medicus Veterinus Vol 10 (5) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.5.758

Penelitian ini dilakukan untuk mengetahui pengaruh manajemen pemeliharaan terhadap munculnya pubertas pada sapi bali dara pada beberapa kelompok ternak di wilayah kerja Puskeswan Sobangan, Kecamatan Mengwi, Kabupaten Badung, Provinsi Bali. Penelitian ini menggunakan 75 ekor sapi bali dara. Penelitian ini merupakan penelitian deskriptif kuantitatif dengan metode survei menggunakan teknik sampling jenuh. Data manajemen pemeliharaan sapi bali dara dan munculnya pubertas diperoleh dengan melakukan wawancara terhadap 51 orang peternak menggunakan kuesioner serta pengamatan secara langsung. Jenis data yang dikumpulkan adalah jenis data primer. Data yang diperoleh dianalisis dengan analisis deskriptif distribusi frekuensi kuantitatif dan dilanjutkan dengan hierarchical cluster dengan umur pubertas sebagai variabel dan manajemen pemeliharaan sebagai objek. Hasil yang diperoleh disajikan dalam bentuk tabel keanggotaan klaster dan grafik dendogram. Hasil penelitian menunjukkan munculnya pubertas pada sapi bali dara pada beberapa kelompok ternak di wilayah Puskeswan Sobangan adalah 21,61 ± 5,24 bulan dengan sebaran terbanyak pada umur 15-20 bulan sebanyak 45,33%. Berdasarkan analisis klaster diperoleh enam keanggotaan klaster dan keanggotaan klaster tidak dipengaruhi oleh manajemen pakan maupun manajemen kandang, hal ini dapat dilihat dari skor manajemen pakan dan manajemen kandang yang tersebar secara merata pada setiap keanggotaan klaster.

Jarak Beranak Sapi Bali pada Kelompok-kelompok Ternak di Wilayah Kerja Pusat Kesehatan Hewan Sobangan, Mengwi, Badung, Bali juliantari, Ni Komang Ade; Laksmi, Desak Nyoman Dewi Indira; Bebas, Wayan
Indonesia Medicus Veterinus Vol 10 (5) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.5.748

Penelitian ini bertujuan untuk mengetahui jarak beranak (calving interval) pada sapi bali menurut tingkat paritas dan body condition score. Total sampel yang digunakan adalah sebanyak 62 sampel. Penelitian ini merupakan penelitian deskriptif, mencari rata-rata calving interval dan dibedakan berdasarkan body condition score. Hasil penelitian menunjukkan bahwa terdapat beragam nilai calving interval. Jika dilihat dari body condition score 2, rata-rata 15,33 ± 0,94 bulan, 13 ± 1 bulan, 14 bulan, 15 bulan, hingga 16,5 ± 0,5 bulan, semakin tua sapi yang diternakkan maka semakin lama kemunculan estrus postpartum sehingga makin panjang juga periode calving intervalnya. Pada body condition score 3, rata-rata 12,91 ± 1,65 bulan, 12,87 ± 1,65 bulan, 12,5 ± 0,5 bulan, 13,25 ± 0,43 bulan, dan 14 bulan. Faktor penyebab panjangnya calving interval dapat dilihat dari segi ternak, peternak, maupun inseminator. Segi ternak yang mempengaruhi yaitu kesehatan ternak. Segi peternak yang mempengaruhi adalah deteksi birahi, pemeliharaan ternak, dan penyapihan yang dilakukan. Segi inseminator adalah prosedur pelaksanaan dan kualitas semen. Hal ini sangat penting karena jika sapi yang diternakkan mengalami faktor-faktor yang disebutkan maka akan mempengaruhi daripada nilai calving interval yang berakibat pada penurunan pendapatan peternak karena jumlah anak yang dihasilkan akan berkurang selama masa produktif.

Waktu Munculnya Birahi Pascamelahirkan pada Sapi Bali di Desa Galungan, Sawan, Buleleng, Bali Yudiani, Putu Mira; Trilaksana, I Gusti Ngurah Bagus; Laksmi, Desak Nyoman Dewi Indira
Indonesia Medicus Veterinus Vol 10 (6) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.6.896

Penelitian ini bertujuan untuk mengetahui waktu munculnya birahi pascamelahirkan/estrus postpartum pada sapi bali yang dipelihara di Desa Galungan. Penelitian ini dilaksanakan dari bulan Desember 2020 hingga Februari 2021 di Desa Galungan, Kecamatan Sawan, Buleleng, Bali. Penelitian ini menggunakan 91 ekor sapi betina (primipara dan pluripara). Data yang didapat kemudian ditabulasikan dan dianalisis secara deskriptif. Hasil analisis didapat bahwa manajemen kandang oleh peternak sapi bali di Desa Galungan yaitu kandang tunggal sebanyak 93,38%, kandang terbuka 69,42%, dan tidak adanya pejantan yaitu 52,06%. Rata-rata estrus postpartum kelahiran pertama yaitu 3,8 ± 1,55 bulan, kelahiran kedua yaitu 3,52 ± 1,48 bulan, kelahiran ketiga yaitu 3,37 ± 1,46 bulan, kelahiran keempat yaitu 3,36 ± 1,42 bulan, kelahiran kelima yaitu 3,35 ± 1,32 bulan, kelahiran keenam yaitu 3,42 ± 1,07 bulan, kelahiran ketujuh yaitu 3,75 ± 1,32 bulan, dan kelahiran kedelapan yaitu 4,33 ± 1,67 bulan. Kelahiran kedua hingga kelima pada estrus postpartum lebih cepat dibandingkan kelahiran pertama, dan estrus postpartum keenam hingga kedelapan mulai lambat. Komposisi pakan yang diberikan oleh peternak ke sapi-sapi mereka yaitu 50% rumput gajah, 19% rumput sertaria, dan 15% daun gamal. Sebagian besar peternak hanya mengandalkan pakan hijauan dan tidak menggunakan pakan tambahan. Rata-rata estrus postpartum kelahiran pertama hingga kelahiran kedelapan pada sapi bali di Desa Galungan yaitu 3,6 ± 1,50 bulan sehingga tergolong terjadi keterlambatan munculnya estrus postpartum (anestrus postpartum).

Hubungan Frekuensi dan Waktu Kawin dengan Jumlah Anak Sekelahiran pada Anjing Golden Retriever yang Dipelihara Di Kota Denpasar Tessa Yuli Oktaviami; Desak Nyoman Dewi Indira Laksmi; I Ketut Puja
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.165 KB)

This retrospective study was conducted to know the relation between time and frequency of mating on litter size of Golden Retriver in Denpasar Region. Mating incident records of 19 Golden Retrievers from 8 kennels were compiled to be analyzed. These data can be use as a reference in effort to maximize the amount of Golden Retriever clans. The result showed that the three times of mating on the 10th , 12th and 14th day after onset of proestrus produced the most litter size 10 ± 2.82. However, mating twice on wice on 9th and 11th after onset of proestrus produced a litter size of 8.50 ± 0.70. The statistic analysis depicted that there was no significant relationship between mating frequencies and mating time on litter size.

Pengaruh Frekuensi Penampungan Semen Terhadap Konsentrasi Dan Abnormalitas Spermatozoa Burung Puyuh Wayan Bebas; Komang Gita Permana; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi
Buletin Veteriner Udayana Vol. 10 No. 2 Agustus 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

The aims of this study are to determine the influence of collecting frequency against concentration and spermatozoa abnormalities of quail. This study used a completely randomized design (CRD) with 3 treatment groups, is collecting sement at 2, 3 and 4 days intervals. Each treatment is repeated 9 times. Observations were made on the concentration of spermatozoa (107 cells/ml) and abnormalities of spermatozoa (%). The data were analyzed using analysis of variance (ANOVA) if there are significantly different (P<0.05) followed by Duncan test. The results showed the concentration of spermatozoa in each treatment respectively 39.00±5.148 x107 cells/ml; 59.49±1.878 x107 cells/ml; 59.11±2.028 x107 cells/ml. Abnormality in each treatment was 14.22±1.394%; 7.22±1.202%; 7.49±1.130%. The frequency of ejaculation was statistically significant (P<0.05) against the concentration and spermatozoa abnormalities of quail.

Kadar Hormon Estrogen pada Sapi Bali saat Pubertas Herdi Wahyu Adi Prananda; Desak Nyoman Dewi Indira Laksmi; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Bali cattle is one of the most important native cattle in Indonesia. There is one measure of reproductive efficiency. The achievement of early age of puberty in accordance with its genetic potential is important to achieve optimum reproductive performance and provide increase in productivity. One of the things that affects the appearance of puberty is the amount of estrogen in the blood serum. The purpose of this study was to determine the levels of estrogen in cattle blood serum. The study was conducted by recording the age first and then taking blood in cattle that had signs of puberty and then carried out checking hormone levels using the ELISA method. The results showed that the average age of puberty was 21.44 months and the average estrogen level at puberty was 84.75 ng / ml. Testing with correlation and regression tests showed a close relationship between age and estrogen levels at puberty with a correlation coefficient of r = 0.798.

Intensitas Estrus Sapi Bali yang Mengalami Silent Heat I Putu Sudana Agustina; Desak Nyoman Dewi Indira Laksmi; I Gusti Ngurah Bagus Trilaksana; Made Kota Budiasa
Buletin Veteriner Udayana Vol. 13 No. 2 Agustus 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

This research was conducted to determine the follicle diameter and estrous intensity of bali cattle that ever had a silent heat in the livestock group of Integrated Farming Systems (SIPADU) in Mengwi Subdistrict Badung Regency, Bali Province, which was implemented in September – Oktober 2019. Bali cattle are grouped into 2 (two) groups; first, the Bali cattle, whichever had silent heat. Second, the Bali cattle whichever had estrous. The results showed the estrous intensity of bali cattle with silent heat showed significant differences (p < 0.05), it is vulva temperature (37,68 + 0,32ºC), vulva diameter (27.64 + 4.28 mm), vulva color (pink, invisible peripheral vessels ) and vulva mucus (no mucus until there was little mucus ). Compared to the group of bali cattle which had estrus, it is vulva temperature (37.96 + 0.51°C), vulva diameter (31,19 + 5,66 mm), vulva color (bright red to dark red and peripheral vessels branching clearly) and vulva mucus (viscous, transparent hang out). The results of the study concluded that there was a difference between the estrus intensity in bali cattle that had silent heat and bali cattle that showed true estrus.

Penambahan Bovine Serum Albumin pada Pengencer Beltsville Thawing Solution terhadap Motilitas dan Daya Hidup Spermatozoa Babi Landrace I Made Agus Suryanatha; Wayan Bebas; Desak Nyoman Dewi Indira Laksmi
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aims to determine the effect of the addition of various bovine serum albumin (BSA) concentrations on betsville thawing solution (BTS)® extender to maintaining vitality and motility of spermatozoa on Landrace pigs. This study used Completely Randomized Design (CRD) with four treatments namely T0 (control): were given BTS® without BSA, T1: were given BTS® added BSA 5 mg/ml, T2: were given BTS® added BSA 10 mg/ml and T3: were given BTS® added BSA 20 mg/ml. Each treatment combination was repeated five times, with the total sample used was 20 samples. The measured variables in this study were the vitality and motility of Landrace pig semen. The results showed that the addition of BSA to BTS® diluents could increase the vitality and motility of spermatozoa on Landrace pigs. Where the optimal concentration was 10 mg/ml in keeping stability and maintaining the osmosis pressure stable

Konsentrasi Spermatozoa Dan Motilitas Spermatozoa Ayam Hutan Hijau (Gallus varius) Wayan Bebas; Desak Nyoman Dewi Indira Laksmi
Buletin Veteriner Udayana Vol. 5 No.1 Pebruari 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.008 KB)

Penelitian ini bertujuan untuk mengetahui “Pengaruh Frekuensi Ejakulasi Terhadap Volume Semen, Konsentrasi Spermatozoa, dan Motilitas Spermatozoa Ayam Hutan Hijau (Gallus varius).Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 kelompok perlakuan. Perlakuan I (T1) : Frekuensi ejakulasi setiap hari atau semen ditampung setiap hari. Perlakuan II (T2) : Frekuensi ejakulasi setiap 2 hari. Perlakuan III (T3) : Frekuensi ejakulasi setiap 3 hari. Masing-masing perlakuan menggunakan 3 ekor ayam hutan dan setiap ekor ayam hutan dilakukan 5 kali ejakulasi. Pengamatan dilakukan tehadap volume semen (ml), konsentrasi spermatozoa (107/ml) dan motilitas spermatozoa (%). Data yang diperoleh dianalisis dengan sidik ragam dan apabila berbeda nyata (P<0,05) dilanjutkan dengan uji Beda Nyata Terkecil.Hasil penelitian menunjukkan bahwa volume semen pada perlakuan T1, T2, dan T3 masing masing : 0,029 + 0,010 ml; 0,049 + 0,010 ml, dan 0,069 + 0,015 ml, konsentrasi spermatozoa masing masing : 61,93 + 4,13 x 107 sel/ml; 84.33 + 3,85 x 107 sel/ml dan 89,80 + 4,24 x 107 sel/ml, dan motilitas spermatozoa masing masing : 77,13 + 2,82; 87+ 2,82% %, dan 97,07 + 1,94%. Secara statistik frekuensi ejakulasi berpengaruh sangat nyata (P<0,01) terhadap volume semen, konsentrasi spermatozoa dan motilitas spermatozoa ayam hutan hijau.

LAKTOSA-ASTAXANTHIN MENINGKATKAN KUALITAS SEMEN BEKU KAMBING GEMBRONG DALAM UPAYA KONSERVASI Wayan Bebas; Wayan Gorda; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; Tjok Gede Oka Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.052 KB)

This research was conducted to produce a formula of diluent for the manufacture of frozen semen of Gembrong goat. Yolk phosphate was used as a basic diluent with the addition of anti-cold shock, antioxidants and combination of anti-cold shock-antioxidant. The research design used a completely randomized design with three kinds of treatments; T1: the addition of lactose 0.6% (anti-cold shock), T2: the addition of astaxanthin 0.004% (antioxidant), and T3: a combination of 0.6% lactose-Astaxanthin 0.004% (combination of anti-cold shock and antioxidants). The addition of DMSO 6% was used in each treatment as intracellular cryo protectants. The freezing process was done with the gradual freezing with conventional techniques. Examination of the quality of semen by thawing prior included progressive motility, viability, and abnormalities. The results showed that anticoldshock-antioxidant combination (0.6% lactose -Astaxanthin 0.004%) produced the best semen quality with progressive motility, viability, and less abnormalities as follow 44.00 + 3.46%, 59.00 + 1.85%, and 14.00 + 0.76% respectively. It was concluded that the addition of a combination of 0.6% lactose-Astaxanthin 0.004% on the phosphate yolk diluent with 6% DMSO as intracellular produced best quality of frozen goat Gembrong semen that suitable for use in artificial insemination and in vitro fertilization.

Co-Authors Achmad Noerkhaerin Putra AJI WAHYU WICAKSONO Annisya Syarifuddin Budi, Josephine Aurora Dhayanti, Ni Luh Evy EVA AGUSTINA SIAHAAN Hanifah Alshofa Nurul Aini Haq, Nurul Ikhsanul Herdi Wahyu Adi Prananda I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Bagus Trilaksana I Ketut Puja I Made Agus Suryanatha I Made Damriyasa I Made Kardena I Putu Sampurna I Putu Sudana Agustina I Putu Suparman I Wayan Bebas I Wayan Gorda juliantari, Ni Komang Ade Komang Gita Permana Kresensia Cyntia Dosom Kristyari, Ni Putu Gita Luh Gde Sri Surya Heryani MADE KOTA BUDIASA Mahasanti, I Gusti Ayu Puji Muhammad Ihwanul Muslimin Ni Luh Eka Setiasih Ni Nyoman Werdi Susari Pridayanti, Ni Kadek Nila Steven Dwi Purbantoro Tessa Yuli Oktaviami Tjok G. O. Pemayun, Tjok G. O. Tjok Gede Oka Pemayun Yudiani, Putu Mira

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