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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Ilmu dan Kesehatan Hewan Jurnal Veteriner Indonesia Medicus Veterinus Buletin Veteriner Udayana
Desak Nyoman Dewi Indira Laksmi
Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB Sudirman, Denpasar, Bali.
Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Pemunculan Pubertas Sapi Bali Dara Peliharaan Kelompok Ternak di Wilayah Kerja Pusat Kesehatan Hewan Sobangan, Mengwi, Badung, Bali Pridayanti, Ni Kadek Nila; Laksmi, Desak Nyoman Dewi Indira; Sampurna, I Putu
Indonesia Medicus Veterinus Vol 10 (5) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.5.758

Abstract

Penelitian ini dilakukan untuk mengetahui pengaruh manajemen pemeliharaan terhadap munculnya pubertas pada sapi bali dara pada beberapa kelompok ternak di wilayah kerja Puskeswan Sobangan, Kecamatan Mengwi, Kabupaten Badung, Provinsi Bali. Penelitian ini menggunakan 75 ekor sapi bali dara. Penelitian ini merupakan penelitian deskriptif kuantitatif dengan metode survei menggunakan teknik sampling jenuh. Data manajemen pemeliharaan sapi bali dara dan munculnya pubertas diperoleh dengan melakukan wawancara terhadap 51 orang peternak menggunakan kuesioner serta pengamatan secara langsung. Jenis data yang dikumpulkan adalah jenis data primer. Data yang diperoleh dianalisis dengan analisis deskriptif distribusi frekuensi kuantitatif dan dilanjutkan dengan hierarchical cluster dengan umur pubertas sebagai variabel dan manajemen pemeliharaan sebagai objek. Hasil yang diperoleh disajikan dalam bentuk tabel keanggotaan klaster dan grafik dendogram. Hasil penelitian menunjukkan munculnya pubertas pada sapi bali dara pada beberapa kelompok ternak di wilayah Puskeswan Sobangan adalah 21,61 ± 5,24 bulan dengan sebaran terbanyak pada umur 15-20 bulan sebanyak 45,33%. Berdasarkan analisis klaster diperoleh enam keanggotaan klaster dan keanggotaan klaster tidak dipengaruhi oleh manajemen pakan maupun manajemen kandang, hal ini dapat dilihat dari skor manajemen pakan dan manajemen kandang yang tersebar secara merata pada setiap keanggotaan klaster.
Jarak Beranak Sapi Bali pada Kelompok-kelompok Ternak di Wilayah Kerja Pusat Kesehatan Hewan Sobangan, Mengwi, Badung, Bali juliantari, Ni Komang Ade; Laksmi, Desak Nyoman Dewi Indira; Bebas, Wayan
Indonesia Medicus Veterinus Vol 10 (5) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.5.748

Abstract

Penelitian ini bertujuan untuk mengetahui jarak beranak (calving interval) pada sapi bali menurut tingkat paritas dan body condition score. Total sampel yang digunakan adalah sebanyak 62 sampel. Penelitian ini merupakan penelitian deskriptif, mencari rata-rata calving interval dan dibedakan berdasarkan body condition score. Hasil penelitian menunjukkan bahwa terdapat beragam nilai calving interval. Jika dilihat dari body condition score 2, rata-rata 15,33 ± 0,94 bulan, 13 ± 1 bulan, 14 bulan, 15 bulan, hingga 16,5 ± 0,5 bulan, semakin tua sapi yang diternakkan maka semakin lama kemunculan estrus postpartum sehingga makin panjang juga periode calving intervalnya. Pada body condition score 3, rata-rata 12,91 ± 1,65 bulan, 12,87 ± 1,65 bulan, 12,5 ± 0,5 bulan, 13,25 ± 0,43 bulan, dan 14 bulan. Faktor penyebab panjangnya calving interval dapat dilihat dari segi ternak, peternak, maupun inseminator. Segi ternak yang mempengaruhi yaitu kesehatan ternak. Segi peternak yang mempengaruhi adalah deteksi birahi, pemeliharaan ternak, dan penyapihan yang dilakukan. Segi inseminator adalah prosedur pelaksanaan dan kualitas semen. Hal ini sangat penting karena jika sapi yang diternakkan mengalami faktor-faktor yang disebutkan maka akan mempengaruhi daripada nilai calving interval yang berakibat pada penurunan pendapatan peternak karena jumlah anak yang dihasilkan akan berkurang selama masa produktif.
Waktu Munculnya Birahi Pascamelahirkan pada Sapi Bali di Desa Galungan, Sawan, Buleleng, Bali Yudiani, Putu Mira; Trilaksana, I Gusti Ngurah Bagus; Laksmi, Desak Nyoman Dewi Indira
Indonesia Medicus Veterinus Vol 10 (6) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.6.896

Abstract

Penelitian ini bertujuan untuk mengetahui waktu munculnya birahi pascamelahirkan/estrus postpartum pada sapi bali yang dipelihara di Desa Galungan. Penelitian ini dilaksanakan dari bulan Desember 2020 hingga Februari 2021 di Desa Galungan, Kecamatan Sawan, Buleleng, Bali. Penelitian ini menggunakan 91 ekor sapi betina (primipara dan pluripara). Data yang didapat kemudian ditabulasikan dan dianalisis secara deskriptif. Hasil analisis didapat bahwa manajemen kandang oleh peternak sapi bali di Desa Galungan yaitu kandang tunggal sebanyak 93,38%, kandang terbuka 69,42%, dan tidak adanya pejantan yaitu 52,06%. Rata-rata estrus postpartum kelahiran pertama yaitu 3,8 ± 1,55 bulan, kelahiran kedua yaitu 3,52 ± 1,48 bulan, kelahiran ketiga yaitu 3,37 ± 1,46 bulan, kelahiran keempat yaitu 3,36 ± 1,42 bulan, kelahiran kelima yaitu 3,35 ± 1,32 bulan, kelahiran keenam yaitu 3,42 ± 1,07 bulan, kelahiran ketujuh yaitu 3,75 ± 1,32 bulan, dan kelahiran kedelapan yaitu 4,33 ± 1,67 bulan. Kelahiran kedua hingga kelima pada estrus postpartum lebih cepat dibandingkan kelahiran pertama, dan estrus postpartum keenam hingga kedelapan mulai lambat. Komposisi pakan yang diberikan oleh peternak ke sapi-sapi mereka yaitu 50% rumput gajah, 19% rumput sertaria, dan 15% daun gamal. Sebagian besar peternak hanya mengandalkan pakan hijauan dan tidak menggunakan pakan tambahan. Rata-rata estrus postpartum kelahiran pertama hingga kelahiran kedelapan pada sapi bali di Desa Galungan yaitu 3,6 ± 1,50 bulan sehingga tergolong terjadi keterlambatan munculnya estrus postpartum (anestrus postpartum).
Hubungan Frekuensi dan Waktu Kawin dengan Jumlah Anak Sekelahiran pada Anjing Golden Retriever yang Dipelihara Di Kota Denpasar Tessa Yuli Oktaviami; Desak Nyoman Dewi Indira Laksmi; I Ketut Puja
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.165 KB)

Abstract

This retrospective study was conducted to know the relation between time and frequency of mating on litter size of Golden Retriver  in Denpasar Region. Mating incident records of 19 Golden Retrievers from 8 kennels were compiled to be analyzed. These data can be use as a reference in effort to maximize the amount of  Golden Retriever clans. The result showed that the three times of mating on the 10th , 12th and 14th day after onset of proestrus produced the most litter size 10 ± 2.82. However, mating twice on wice on 9th and 11th after onset of proestrus produced a litter size of 8.50 ± 0.70. The statistic analysis depicted that there was no significant relationship between mating frequencies and mating time on litter size.
LAKTOSA-ASTAXANTHIN MENINGKATKAN KUALITAS SEMEN BEKU KAMBING GEMBRONG DALAM UPAYA KONSERVASI Wayan Bebas; Wayan Gorda; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; Tjok Gede Oka Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was conducted to produce a formula of diluent for the manufacture of frozen semen of Gembrong goat. Yolk phosphate was used as a basic diluent with the addition of anti-cold shock, antioxidants and combination of anti-cold shock-antioxidant. The research design used a completely randomized design with three kinds of treatments; T1: the addition of lactose 0.6% (anti-cold shock), T2: the addition of astaxanthin 0.004% (antioxidant), and T3: a combination of 0.6% lactose-Astaxanthin 0.004% (combination of anti-cold shock and antioxidants). The addition of DMSO 6% was used in each treatment as intracellular cryo protectants. The freezing process was done with the gradual freezing with conventional techniques. Examination of the quality of semen by thawing prior included progressive motility, viability, and abnormalities. The results showed that anticoldshock-antioxidant combination (0.6% lactose -Astaxanthin 0.004%) produced the best semen quality with progressive motility, viability, and less abnormalities as follow 44.00 + 3.46%, 59.00 + 1.85%, and 14.00 + 0.76% respectively. It was concluded that the addition of a combination of 0.6% lactose-Astaxanthin 0.004% on the phosphate yolk diluent with 6% DMSO as intracellular produced best quality of frozen goat Gembrong semen that suitable for use in artificial insemination and in vitro fertilization.
Paparan Formalin Menghambat Proses Spermatogenesis pada Mencit Luh Gde Sri Surya Heryani; Ni Nyoman Werdi Susari; I Made Kardena; Desak Nyoman Dewi Indira Laksmi
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.505 KB)

Abstract

Formaldehyde is one of the exogenous free radicals. During formaldehyde exposure, there will be morefree radical or reactive oxygen compound formed through electron transport chain. Excessive reactiveoxygen compound triggers the process of lipid peroxide reaction in the membrane of spermatozoa cell. Theaims of this study were to find out that formaldehyde exposure caused abnormalities in the number of thenumber of spermatogonium type A, spermatogonium type Pachytene, 7th spermatid and 16th spermatid inspermatogenesis process.This study was experimentally and randomly pretested-posttested- with controlgroup design. The samples of this study were adult male mice strain Balb-C (age 2-3 months) with thefollowing criteria: body weight between 22-25 grams and healthy. Randomly, 48 mice were divided intothree groups, were control group, the first treatment group and the second treatment group. Prior to thetreatment, were taken a half from each group for the pre-test, by preparing microscopic preparation testicleand examination was performed to the total spermatogenic cells. The rests of the mice were used as posttestexamination after 35 days treatment. On the 36th day, all the rest mice were necropsied for microscopictesticle preparation. The result of this study showed that the formaldehyde exposure caused significantlydecrease in the number of spermatogenic cells (p<0,05), the average of type A spermatogonium cells atcontrol group, 1st treatment group and 2nd treatment group were 39,90±0,51; 20,42±0,72; 15,65±0,88respectively; spermatogonium type Pachytene were 48,47±1,28; 32,60±3,06; 23,14±3,16 respectively; 7thspermatid were 97,47±5,28; 39,98±4,28; 30,36±2,96 respectively and16th spermatid were 73,08±4,05;21,70±1,70; 16,38±1 respectively.It can be concluded that the formaldehyde exposure decreased the amountof spermatogenic cells. The result of this study is expected to be used as the baseline for further study inorder to measure formaldehyde content as a free radical in testicle .
Pemberian Ekstrak Oncom Hitam dan Merah Memperpanjang Siklus Estrus dan Mempertebal Endometrium Tikus Putih Hanifah Alshofa Nurul Aini; Desak Nyoman Dewi Indira Laksmi; Ni Luh Eka Setiasih; Steven Dwi Purbantoro
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.714 KB) | DOI: 10.19087/jveteriner.2020.21.4.565

Abstract

hytoestrogens are active compounds, derived from plants, which have a similar structure and function as estrogen. Phytoestrogens are commonly found in legumes. Oncom, which is assumed containing phytoestrogens, is one of the most famous legumes food from Indonesia and widely consumed daily in West Java. This study was aimed to determine the effect of oncom extract on estrous cycle, endometrium thickness, and the number of antral follicles in productive age rats (Rattus novergicus). This experimental study was using 21 three-to-four-month-old fertile female rats and divided into three groups. Group I (K) was considered as a control group without any treatment. Group II and III were treatment groups which were given black (H) and red (M) oncom extracts 0.005 g/g body weight, respectively, orally with a feeding tube for 14 days. The length of the estrous cycle was measured by performing vaginal swab with interval 12 hours after first treatment was given and during the treatment. Endometrium thickness and the number of antral follicleswere measured by collecting the organs uterus and ovary for histological purpose with paraffin method after rats were euthanized post-treatment oncom extracts for 14 days. Data were analyzed by ANOVA and continued with LSD test. The total length of estrous cycle of control group, black oncom extract group, andred oncom extract group was 107,43±3,16 hours, 141,43±15,36 hours, and 161,14±17,10 hours, respectively. The mean of endometrium thickness of control group, black oncom extract group, and red oncom extract group was 346,945±65,88 ?m, 485,740±86,69 ?m, and 533,904±78,93 ?m, respectively. The number ofantral follicles of control group, black oncom extract group, and red oncom extract group was 6,00±1,54, 8,43±2,99, and 9,14±2,72, respectively. Results showed that black and red oncom extracts had a significant effect on the length of estrous cycle and endometrium thickness in rats, yet there is no significant difference in the number of antral follicles. In summary, black and red oncom extracts had effects on the length of estrous cycle and endometrium thickness, yet there was no effect on the number of antral follicles.
Viabilitas Spermatozoa Ayam Hutan Hijau dalam Pengencer Posfat Kuning Telur Ditambah Laktosa pada Penyimpanan 5oC (GREEN JUNGGLE FOWL SPERM VIABILITY IN EGG YOLK PHOSFAT DILUENTS CONTAINING LACTOSE STORAGED AT 5OC) Wayan Bebas; Desak Nyoman Dewi Indira Laksmi
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.855 KB)

Abstract

The objective of this study was to determine the addition of lactose on egg yolk phosphate diluentsstored at 5°C to the plasma membrane intact, acrosome membranes intact of green jungle fowlspermatozoa, fertility and hatchability of chicken eggs. This study used completely randomized designwith three treatment groups. Treatment I (T0): egg yolk phosphate diluents only (control), treatment II(T1): egg yolk phosphate diluents + lactose 0.3%, and treatment III (T2): egg yolk phosphate diluents +lactose 0.6 %. Each group was replicated nine times. Semen was collected from eight green jungle fowl andevaluated both macroscopically and microscopically. Semen was diluted with each treatment with a singleinsemination dose (0.5 mL) with a concentration of 150 million progressively motile cells. Once diluted,semen was stored at 3-5°C for 48 hours and then was observed for the plasma membrane of intact,acrosome membrane of intact, and some semen were inseminated on local female chicken village forfertility, and hatchability eggs observation. The results showed that the addition of lactose 0.3% and 0.6%providing significant increase (P <0.05) on plasma membrane intact, acrosome membrane intact, fertilityand egg hatchability when compared to controls. However the addition of 0.3% and 0.6% lactose did notshowed a significant effect (P> 0.05). This study concluded that the addition of lactose on egg yolk phosphatediluents for storing green jungle fowl spermatozoa at 3-5oC for 48 hours can improve the plasma membraneintact, acrosome membranes intact, fertility and hatchability of chicken eggs.
QUALITY OF BERKSHIRE BOAR SEMEN DILUTED WITH A COMBINATION OF BELTSVILLE THAWING SOLUTION AND MELATONIN AT DIFFERENT STORAGE TIMES Pradnyani, Gusti Ayu Putu Indira; Bebas, Wayan; Pemayun, Tjok Gde Oka; Trilaksana, I Gusti Ngurah Bagus; Laksmi, Desak Nyoman Dewi Indira; Susari, Ni Nyoman Werdi
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p29

Abstract

Berkshire pigs are a popular choice for small and large scale pig farming enterprises worldwide, including in Bali. Berkshire pigs are well known for their superior meat quality and relatively fast growth rate. This study aimed to determine the effect of storage duration on the quality of Berkshire boar spermatozoa diluted with Beltsville Thawing Solution (BTS®) supplemented with melatonin. Semen from Berkshire boars was diluted using BTS® supplemented with melatonin at a concentration of 1.0 mM and stored at a temperature of 15 –20°C. Semen quality was evaluated at different storage intervals: (P1) 48 hours, (P2) 60 hours, and (P3) 72 hours. The observed semen quality parameters included sperm motility, sperm abnormalities, sperm viability, and plasma membrane integrity of spermatozoa, which were examined using smear preparations observed under a microscope. In addition, sperm malondialdehyde (MDA) levels as an indicator of oxidative stress were measured using the Porcine MDA ELISA Kit. Duncan’s multiple range test analysis showed that the storage duration of Berkshire boar semen diluted with BTS® supplemented with melatonin was able to maintain sperm motility, abnormality, viability, plasma membrane integrity, and malondialdehyde levels up to 72 hours of storage, with sperm motility of 40.66%, sperm viability of 53.00%, and sperm abnormalities of 6.33%. These values met the minimum standards set by the Indonesian National Standard (SNI 8034:2023) for liquid boar semen. These results indicate that melatonin acts as an antioxidant capable of neutralizing free radicals, thereby inhibiting the occurrence of oxidative stress in spermatozoa cells.
Co-Authors Achmad Noerkhaerin Putra AJI WAHYU WICAKSONO Annisya Syarifuddin Budi, Josephine Aurora Dhayanti, Ni Luh Evy EVA AGUSTINA SIAHAAN Hanifah Alshofa Nurul Aini Haq, Nurul Ikhsanul Herdi Wahyu Adi Prananda I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Bagus Trilaksana I Ketut Puja I Made Agus Suryanatha I Made Damriyasa I Made Kardena I Putu Sampurna I Putu Sudana Agustina I Putu Suparman I Wayan Bebas I Wayan Gorda juliantari, Ni Komang Ade Komang Gita Permana Kresensia Cyntia Dosom Kristyari, Ni Putu Gita Luh Gde Sri Surya Heryani MADE KOTA BUDIASA Mahasanti, I Gusti Ayu Puji Muhammad Ihwanul Muslimin Ni Luh Eka Setiasih Ni Nyoman Werdi Susari Pradnyani, Gusti Ayu Putu Indira Pridayanti, Ni Kadek Nila Steven Dwi Purbantoro Tessa Yuli Oktaviami Tjok G. O. Pemayun, Tjok G. O. Tjok Gde Oka Pemayun Tjok Gede Oka Pemayun Yudiani, Putu Mira