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STRUKTUR GENETIK DAN FILOGENI YELLOWFIN TUNA (THUNNUS ALBACARES) BERDASARKAN SEKUEN DNA MITKONDRIA CONTROL REGION SITOKROM OKSIDASE I PADA DIVERSITAS ZONE BIOGEOGRAFI I Made Sara Wijana; I Gusti Ngurah Mahardika
Bumi Lestari Journal of Environment Vol 10 No 2 (2010)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

Genetic structure and phylogeni of 37 sequences control region DNA mitochondrial cytocrome oxidase I of yellowfin tuna (Thunnus albacores) have been downloaded from GeneBank and analised using Maximum Likehood (ML), Pairwise Genetic Distance and Bootstrapping Phylogeni Model of Kimura 2 Parameter. The result shows that the corrected value of the data was 5,5% and the mean of genetic distances was 3,7 %, where the shortest distance was 0.0% and the longest was 5%. The genetic distances with the out group (Thunnus obesus) ranged between 7,8 % – 9,8 % and with the Thunnus thynus ranged between 10,4% – 12,5%. The value of bootstrap phylogeny of 37 sequences of yellowfin tuna was less than 50%. All those results shows that there was no significant genetic differences of 34 samples sourced from Philipines and 3 from Spain based on sequence region DNA mitochondrial cytocrome oxidase I.
STUDI KUALITAS PERAIRAN BEBERAPA SUNGAI DI KABUPATEN BADUNG I Made Sara Wijana
Bumi Lestari Journal of Environment Vol 3 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on water quality of some rivers in Badung Regency (Dangkang River inBeringkit, Penet River in Ayunan, Mati River in Kuta, as well as Ayung River in Carang Sariand Bongkasa) was carried out in dry and wet seasons 2002. Aim of the study is to find thewater quality of those rivers benchmarked with Governor of Bali decree (decision) No.515/2000.Results of the study showed that water quality of Mati River is the worst. There areseven parameters in this river which did not pass the benchmark, namely odor, taste,phosphate, DO, fat, Fecal coli (E. coli) and coliform. Meanwhile, Ayung river had threeparameters (phosphate, fat and TDS), and Penet as well as Dangkang Rivers had twoparameters only (phosphate and fat) which didi not pass the benchmark. Water from all riverare found to be polluted by phosphate and fat either during dry or wet seasons.
Status Mutu Air Sungai Unda Pada Lahan Eks Galian C Kabupaten Klungkung I Made Sara Wijana; Abd. Rahman As-syakur; Made MS Mahendra; I GA Kunti Sri Panca Dewi
Bumi Lestari Journal of Environment Vol 20 No 2 (2020)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2020.v20.i02.p02

Abstract

The Unda River (Tukad) is the second largest river in Bali Province after the Ayung River. The Unda River has been used for various activities, such as tourism activities for white water rafting and agricultural irrigation activities. In addition, Unda River water is planned to be used as a raw water source in the planned construction of the Bali Cultural Center (PKB) which is planned to be built in the lower reaches of the Unda River. Therefore, it is necessary to conduct research related to the water quality of the Unda River, especially in the Lower Unda River. Water quality analysis refers to class II water quality standards according to the Bali Governor Regulation Number 16 of 2016. Meanwhile, the analysis of water quality status uses the pollution index (IP) according to the Decree of the Minister of the Environment Number 115 of 2003. The results of the analysis show that water quality parameters that exceed the highest quality standard is downstream (sampling point 3) with 4 (four) parameters, namely Total Dissolved Solid (TDS), chemical oxygen demand (COD), total coliform and fecal coli. While the sampling points 1 (one) and 2 (two), the parameters that exceed the quality standard are total coliform and fecal coli. In addition, the results of this study also show that the pollution index value downstream is increasing, where at sampling point 1 which is a DAM location has a pollutant index of 3.78 (light pollution) and at sampling point 2 of 6.27 (moderate pollution) and at sampling point 3 of 7.67 (medium polluted).
Checklist, Indeks Ekologi, dan Status Konservasi Komunitas Fauna Akuatik di Kawasan Sungai Unda dan Sekitar Pantai Jumpai, Kabupaten Klungkung, Provinsi Bali Gede Surya Indrawan; Putu Angga Wiradana; I Made Saka Wijaya; Abd. Rahman As-syakur; M. Rheza Rizki Syahputra; I Made Sara Wijana
Bumi Lestari Journal of Environment Vol 21 No 1 (2021)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2021.v21.i01.p02

Abstract

Aquatic organisms or nekton have an important role in supporting the stability of the aquatic environment and human life. This study aims to determine the nekton type community, ecological index, and their conservation status in the Unda River area and around Jumpai Beach, Klungkung Regency, Bali. The study was conducted in July - August 2020. The study was conducted in 2 communities, namely Macrozoobenthos and Nekton. Macrozoobenthos samples were collected using the quadratic method in the Jumpai Beach area and the direct observation method along the Unda River. Nekton communities are collected through primary data using "survey methods" and secondary data through interviews with fishermen and local communities. The ecological index data were analyzed using the Shannon-Weiner diversity index (H'), dominance index (C), and uniformity index (E), while the data on Nekton type and conservation status were analyzed descriptively. The nekton species community in the waters of the Unda River and Jumpai Beach areas was obtained as many as 26 species in 21 families which were dominated by Kepala Timah (Aplocheilus panchax) and sepat rawa (Trichogaster trichopterus), while Nautica sp for the Macrozoobenthos community. The ecological index of the aquatic community is the Shannon-Weiner Diversity Index (H') value of 2.98 (moderate), the wealth index (R) of 3.95 (moderate), and the evenness index (E) of 1.21. (High). The highest percentage ratio of nekton communities was obtained by the Macrozoobenthos community, namely 53%, while the nekton species was 46%. Conservation status shows that 13 species are included in the LC (Least Concern) category, 12 species are included in the NE (Not Evaluated) category, and 1 species are included in the VU (Vulnerable) category.
STUDI DAN PEMANTAUAN KUALITAS AIR DI DAERAH ALIRAN SUNGA! (DAS) BADUNG I Made Sara Wijana; I.G.A. Kunti Sri Pancadewi; Abd Rahman As-syakur
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 7 No 1 (2012)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

Badung River is one of the river that across Denpasar city. As one of the rivers that passes through the city with a very dense population, along the river there are activities that has potentially degrade water quality. The aims of this study are to determine the water quality trend and status of water quality standards of Badung River from 2006 to 2009. The result analysis showed the water quality on Badung River declined in the upstream section and relatively stabl e in the middle and downstream section. This situation is result of the waste sources is relatively constant throughout the study period. The existence of DO, BODS, Nitrites, Phosphates, Coliform, and E. Coli is the water quality parameter that always exceeds the threshold of quality standard.
STATUS MUTU AIR SUNGAI AYUNG BERDASARKAN DATA PEMANTAUAN KUALITAS AIR TAHUN 2014-2018 I Made Sara Wijana; Ni Made Ernawati; Abd Rahman As-syakur
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 14 No 2 (2020)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/EJES.2020.v14.i02.p05

Abstract

It is very important to know the status of river water quality, in order to determine the direction of resource management which is decreasing in quality according to its allocation. Analysis of the status of the water quality of the Ayung River in Bali Province using secondary data in the form of data on illness results from 2014 to 2018. The number of parameters applied to the fire is 16 parameters with class II water quality standards referring to attachment XII of Bali Governor Regulation No. 16 of 2016 concerning Environmental Quality Standards and Environmental Damage Standard Criteria. The analysis used the Storetic Method (Decree of the Minister of Environment Number 115 of 2003). The results of the analysis show that the status of Ayung River water is at very good, good and moderate levels. The status of the Ayung River's air quality tends to decline from year to year and from upstream to downstream. Status determining parameters are: BOD5, phosphate, phenol, detergent and sulfide. Key words: water quality status; ayung river; storet.
KEANEKARAGAMAN LAMUN DAN MAKROZOOBENTOS SEBAGAI INDIKATOR KONDISI PERAIRAN PANTAI SINDHU, SANUR, BALI I Made Sara Wijana; Ni Made Ernawati; Made Ayu Pratiwi
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 13 No 2 (2019)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.076 KB) | DOI: 10.24843/EJES.2019.v13.i02.p11

Abstract

Sindhu Beach is part of the Sanur beach area. Sanur area is one of 11 strategic national tourism areas in Bali. Beach tourism activities have the potential to cause degradation of coastal ecosystems. Ecosystem changes can be determined using bioindicators. This study aims to determine the condition of the Sindhu Coast waters ecosystem based on seagrass diversity and macrozoobenthos as indicators. This research was conducted in November 2019 at Sindhu Beach. Seagrass observations and macrozoobenthos sampling were carried out at four stations with three replications at each station. Data and samples were collected using the quadratic transect method. Eight species of seagrass were found in Sindhu Beach with a percentage of cover between 16.33% - 63.37% and density between 1572 ind / m2 - 3314.4 ind / m2. The average value of the seagrass diversity index was 1.24, the average uniformity index was 0.78, and the average dominance index was 0.32. Types of macrozoobenthos found were 23 species with abundances ranging from 2.33 ind / m2 - 28.33 ind / m2. The average value of macrozoobenthos uniformity index is 1.50, the average diversity index is 0.68 and the average dominance index is 0.36. Based on the index value shows that the waters of Sindhu Beach are in good condition or there is no ecologically significant pressure.
BIOLOGICAL ASPECTS, CATCHING ASPECTS AND FISHING GROUND OF EASTERN LITTLE TUNA OR KAWAKAWA(Euthynnusaffinis (Cantor, 1849)) BASED ON THE FISHING GEAR AT WPP 572 Gussasta Levi Arnenda; Bram Setyadji; Ngurah Intan Wiratmini; I Made Sara Wijana
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 16, No 3 (2020): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.16.3.%p

Abstract

Eastern little tuna (Euthynnus affinis) is the main catch in WPP572. The caught E. affinis are still in their infancy. This research aims is to know the biological aspects, the fishing aspects and the distribution of the fishing gear per fishing gear, as a consideration for sustainable fisheries management. The research was carried out during the years 2019-2020. Taking samples for gonad observation was carried out by direct survey to the location in February, May, July and October 2019. The measurement of fish length was carried out in stratified random. Catching data were retrieved by requesting secondary data and interviews with related parties. The distribution of fishing grounds is mapped using the QGIS 3.4, the spatial distribution of catches is mapped. This study showed the size structure of E. affinis on purse seine, troll line and encircling gill netfishing gear from 16-55 cm FL, while on the boat/raft lift net it was 21-50 cmFL. These fish growth's is positive allometric on purse seine and encircling gillnet fishing gear, negative allometric on boat/raft lift net fishing gear, and isomeric on troll line. The sex ratio is in the ratio 1: 1.15 and balanced. Based on the maturity level, the gonads were mature in July and October and immature in February and May. Gonads ripened for the first time at a size of 42.032 cmFL. The distribution of the purse seine catchment areas at 3°-6°N and 93°-96°E, and 3°-6°S and 94°-95°E. Boat/raft lift net at 0°-3°S and 95° -101°E. Troll line 4°-6°N and 94°-95 E. Encircling gillnet 0°-2°S and 99°-101°E.
Kajian Struktur Histologi Hati, Insang dan Lambung Ikan Nila (Oreochromis niloticus) di Danau Batur, Bangli Ida Ayu Putu Sugiantari; A. A. S. A. Sukmaningsih; I Made Sara Wijana
Buletin Anatomi dan Fisiologi Volume 7, Nomor 1, Tahun 2022
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.7.1.2022.51-59

Abstract

Danau Batur saat ini sudah mulai tercemar akibat tingginya beban pencemaran yang masuk ke perairan disebabkan oleh aktivitas masyarakat di sekitar danau. Penelitian ini bertujuan untuk melihat kerusaka struktur histologi hati, insang, dan lambung, ikan nila serta mengetahui jenis logam berat yang terdapat di perairan Danau Batur. Penelitian ini menggunakan 5 titik lokasi pengambilan sampel ikan nila. Sampel dibuat sayatan histologi organ dengan metode embedding dan analisis logam berat dengan Atomic Absorption Spectrophotometer. Hasil skoring pada insang luar, diperoleh kejadian patologi berturut-turut hiperplasia, edema lamela sekunder, dan fusi lamela paling berat 25% pada 4 lokasi, 18,7% pada lokasi 5, dan 25,00% pada lokasi 2. Pada insang dalam menunjukkan berturut-turut hiperplasia, edema lamela sekunder dan fusi lamela paling berat dengan frekuensi masing-masing 25,00% pada 4 lokasi, 25,00% pada lokasi 5, dan 25,00% pada lokasi 4. Lambung ditemukan kerusakan berupasel kariolisis, infiltrasi sel radang, dan desquamasi epitel. Histologi hati menunjukkan adanya kariolisis, karioreksis, dan degenerasi melemak. Konsentrasi logam berat di organ hati terdeteksi Fe dengan konsentrasi paling besar 3,96 ppm pada lokasi 2 dan Cu dengan konsentrasi paling besar 0,7 ppm pada lokasi 5. Ditemukannya kerusakan organ serta adanya logam berat, menandakan bahwa perairan Danau Batur mengalami pencemaran oleh aktivitas manusia di sekitar danau. Lake Batur is currently starting to be polluted due to the high pollution load enters into waters which caused of the people activities around the lake. This study aims to observe the histological structure damage of the liver, gills, and stomach, on the tilapia fishes, as well as to determine heavy metal types found in the Batur Lake water. This study was carried out 5 points of Batur Lake. The fish samples were prepared histological section by using the embedding method. Heavy metals were determined by using the Atomic Absorption Spectrophotometer. The scoring on the external gills showed there were pathological occurrences of hyperplasia, secondary lamellae edema, and lamellae fusion with most severe frequency of 25% at 4 locations, 18.7% at location 5, and 20% at location 2 respectively. Internal gills showed hyperplasia, secondary lamellae edema and most severe lamella fusion with most severe frequency of 25% at 4 sites, 25% at location 5, 25% at location 4 respectively. The stomach damaged were karyolytic cells, inflammatory cell infiltration, and epithelial desquamation. Liver histology showed karyolysis, karyorrhexis, and fatty degeneration. The heavy metals Fe and Cu were detected in the liver with Fe with the highest concentration of 3.96 ppm (Fe) at location 2 and 0.7 ppm (Cu) at location 5. The discovery of organ damages and the presence of heavy metals on the tilapia fish body indicated that Batur Lake water has been polluted by human activity around the lake. 
PROFIL BEBERAPA KOMUNITAS PERAIRAN TELUK BANYUWEDANG I Made Sara Wijana; Ni Made Ernawati
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 16 No 1 (2022)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/EJES.2022.v16.i01.p10

Abstract

Banyuwedang Bay is located in the West Bali National Park (TNBB), which is part of the conservation area. There were a lot of tourism activities developed in this area, which potentially caused disruption of the surrounding water conditions. This study aims to determine the profile of various communities in the waters of Banyuwedang Bay and as initial data to see the development of these communities due to the environmental impacts of various activities in the waters and coastal area of Banyuwedang Bay. Data was collected from five observation stations including: Community structure of Plankton, Macrozoobenthos, and seagrass, and coral reef cover. Result shows that the phytoplankton community consist of 9 genera where Nitzschia sp. (Bacillariophyceae) as the dominant species found. Phytoplankton have low diversity index ranging between 0.348 - 1.411. Zooplankton diversity index also has low value 0.693 with three genera found namely Copepoda sp., Nauplius sp. and Daphnia sp. Macrozoobenthos community is composed of 17 macrozoobenthos genera, with low diversity index ranging ??between 0.562-1.809. There are only two species of seagrass, Halophila ovalis and Cymodocea rotundata, which were found in Banyuwedang Bay. Coral reef cover seems to be ranged from poor to good conditions, with 25% - 73% coverage. Based on the result, we concluded that the Banyuwedang Bay has a moderate condition to the growth of plankton and seagrass, but good for coral reefs. Periodic monitoring is needed to find out changes in conditions that can occur in Banyuwedang Bay. Keywords: Banyuwedang Bay; community structure; macrozoobenthos