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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Zuriat BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Agro Jurnal Sumberdaya Hayati Indonesian Journal of Science and Technology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Celebes Biodiversitas : Jurnal Sains dan Pendidikan Biologi Jurnal Biologi Tropis Menara Perkebunan Jurnal Jamu Indonesia EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Indonesian Journal of Jamu
Diah Ratnadewi
Biology Department, Fac. Mathematics And Natural Sciences, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Journal : JURNAL BIOLOGI INDONESIA

 1 
Induksi Poliploidi pada Tanaman Talas (Colocasia esculenta (L.) Schott) Kultivar Kaliurang dengan Perlakuan Kolkisin secara In Vitro Ermayanti, Tri Muji; Wijayanta, Ardian Nur; Ratnadewi, Diah
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.917 KB) | DOI: 10.14203/jbi.v14i1.3667

Abstract

ABSTRACTGenetic modification to increase productivity and other better growth characteristics of Kaliurang taro could be done by various methods; one of them is polyploid induction. Kaliurang taro has performed excellent traits, it is resistant to leaf blight disease and pests. Polyploid plants of Kaliurang taro are expected to have excellent char-acteristics and increased productivity. The objective to this study was to obtain an efficient method through in vitro induction of polyploidy using colchicine on Kaliurang taro. Aseptic plantlets of Kaliurang taro were soaked in colchicine solution at 0.05, 0.1 and 0.2% for 1, 2 and 3 days. Control was untreated plantlets. Each treatment consisted of 12 replicates. The colchicine-treated plantlets were then planted on MS medium containing 2 mg/L BAP, 1 mg/L thiamine and 2 mg/L adenine. Survival rate and vegetative growth of plantlets were observed every week for 8 weeks after planting. The results showed that increasing colchicine concentra-tion and the soaking period produced planlets with various survival rate. Flowcytometric analysis indicated that the treatment of soaking the plantlets in 0.05% colchicine for 1 day resulted in 14.3% of tetraploid plantlets. The most efficient of colchicine treatment was 0.2% for 3 days, resulting in 57.1% tetraploids, with the efficient value of 33.3%. There was chromosome multiplication from diploid to tetraploid which was confirmed through both flowcytometric analysis and chromosomes counting.Keywords: Taro (Colocasia esculenta L.), Kaliurang, in vitro, flowcytometer, chromosome multiplication, tetraploids
INDUKSI POLIPLOIDI TANAMAN KANGKUNG (IPOMOEA AQUATICA FORSSK.) KULTIVAR SALINA IN VITRO DENGAN ORYZALIN Rahmi, Putri; Witjaksono, Witjaksono; ratnadewi, diah
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3760

Abstract

ABSTRACTWater spinach is a vegetable plant consumed by people all over the world. Its small morphology requires improvement to increase the size and productivity. An alternative way to do that is by increasing the ploidy of its chromosome. This paper describes in vitro induction of polyploidy of water spinach. Inoculum of in vitro seedling, in vitro shoots and nodal stem segments were immersed in MS solution containing oryzalin at concentrations of 0.00, 1.25, 2.50, 3.75 and 5.00 µM with immersion duration of 4, 8 and 24 hours. Oryzalin treatments reduced the growth variables of the in vitro shoots of all inocula tested, compared to control of no oryzalin treatment.  On immersion of 24 hours and high concentration of 5.00 µM, many inoculum failed to grow to the third passage of subculture. The best growth of in vitro shoot to the third passages occurred in the treatment combination of oryzalin 1.25 µM-8 h immersion and 2.50 µM-4 h immersion for seedling inoculum, treatment combination of 3.75 µM-4 h immersion for shoot tip inoculum and 1.25 µM-4 h immersion for nodal stem segment, compared to other treatments. Analysis of flow cytometry on 41 leaf samples from oryzalin treatment derived shoots showed 14.63% tetraploid, 36.59% mixoploid and 48.78% diploid. The efficiency of tetraploid formation reached  60%, provided only by the treatment of oryzalin 1.25 µM-8 h on seedling segments inoculum. Tetraploid shoots need to be proliferated, acclimatized, grown into planting materials and planted for agronomical analysis to provide evidence whether or not tetraploid water spinach is viable for commercial cultivation. 
EVALUASI PERTUMBUHAN STEVIA REBAUDIANA BERT. TETRAPLOID SECARA IN VITRO DAN DI LAPANG UNTUK PRODUKSI STEVIOSIDA DAN REBAUDIOSIDA-A Adabiyah, Rifatul; Ratnadewi, Diah; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3809

Abstract

ABSTRACTGenetic improvement through tetraploid induction of Stevia rebaudiana is important in order to increase the sweetener content, steviol glycoside. Tetraploid plants of several species after induction with colchicine and oryzalin have higher growth and secondary metabolite contents compared to the diploid plants. This study was aimed to evaluate growth as well as their stevioside and Reb-A content of S. rebaudiana tetraploid and diploid (control) plants cultured in vitro and grown in the field after acclimation process. This study used 3 tetraploid clones, namely B60.3H8, P1T22, P3T5, and 1 diploid clone as control. Shoot tips were cultured on MS medium without addition of plant growth regulators for 6 weeks, then they were acclimated in a greenhouse, followed by planting them in the field. Growth of shoot culture, plantlets in the greenhouse and plants in the field were observed. Analysis of stevioside and Reb-A was done by HPLC. The results showed that plantlets of diploid clone had higher in vitro growth and survival rate in the greenhouse than that of tetraploids. Tetraploid clone P1T22 had similar growth as diploid plants, but higher than the growth of tetraploid B60.3H8 and P3T5. Fresh and dry weights of B60.3H8 was similar with diploid plants, but higher than P1T22 and P3T5 tetraploid clones. The highest level of stevioside and Rebaudiosida-A was found in tetraploid B60.3H8 clone, the lowest was found in the diploid plants. The highest ratio of stevioside : Reb-A was found at B60.3H8 tetraploid clone.  Keywords: Stevia rebaudiana, in vitro, field, growth, Stevioside, Rebaudioside-A, tetraploid  
Co-Authors . Suharyo . SUMARYONO . TRIVADILA Adabiyah, Rifatul Ai Nurhasanah Husnul Izzati Amallia, Nur Anggraini Barlian Aris Tjahjoleksono Atik Choirul Hidajah C Hanny Wijaya Christofora Hanny Widjaja Dian Rahma Pratiwi Dorly Dorly Dyah Iswantini Dyah Retno Wulandari Dyah Retno Wulandari Edhi Sandra Efah FITRAMALA Elsa May Susanti ENDANG HADIPOENTYANTI Erwin Al Hafiizh Eva KHAERUNNISA Gustania, Leolita Hadi SUNARSO Hari Basuki Notobroto Hasibuan, Yustiny Andaliza Hayati MINARSIH Ika Dewi Ana Imron RIYADI Khalisa Aini Sinaga Khalisa Aini Sinaga Leksonowati, Aryani Lilis Solihat Mas’ud, Zainal Alim Maulidiyah Utami Muhamad Ayi Pandu Perdana Nina Ratna Djuita Nur Amallia Nurmuliayanti Muis Nurmuliayanti Muis Nur`Aeni, Fitri Rahmi, Putri Rifatul Adabiyah Rinny Saputri Rizka Musdalifah Amsar Rukmi, Paramita Sadha Dayinta Rupa, Darius RUSMILAH SUSENO Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirjo Soekisman Tjitrosoedirjo Sulistyaningsih, Yohana Caecilia Sumaryono . Titiek Setyawati Titiek Setyawati Titiek Setyawati Titiek Setyawati Tjitrosoedirdjo, Soekisman Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Triati Dewi Kencana Wungu Wijayanta, Ardian Nur WIRDHATUL MUSLIHATIN Witjaksono Witjaksono WOLFGANG FRANK Yohana Caecilia Sulistyaningsih Yustiny Andaliza Hasibuan Zainal Alim Mas’ud Zainal Alim Mas’ud