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All Journal Bioscience Bioeducation Journal Jurnal Biologi Tropis Acta Biochimica Indonesiana Atrium Pendidikan Biologi Pelita Eksakta Jurnal Serambi Biologi
Irma Leilani Eka Putri
Universitas Negeri Padang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Physics Other

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Pseudomonas fluorescens as Plant Growth Promoting Rhizobacteria (PGPR) Atiqahh Muthiah; Linda Advinda; Azwir Anhar; Irma Leilani Eka Putri; Siska Alicia Farma
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The continuous use of chemical fertilizers will cause negative impacts such as pollution of the agricultural environment. This needs to be addressed by using Plant Growth Promoting Rhizobacteria (PGPR) which can trigger plant growth. Pseudomonas fluorescens is a bacterium that lives in the rhizosphere and is included in PGPR bacteria. P. fluorescens has the ability to produce siderophores, IAA, dissolve phosphate and fix nitrogen. Therefore, these bacteria can increase growth, provide plant nutrients and inhibit pathogens that cause plant diseases. This study aims to collect and analyze articles related to Pseudomonas fluorescens as PGPR. This type of research is research that uses the literature review method and literature collection using internet sources from the Google Scholar database. This stage consists of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that P. fluorescens as PGPR can increase plant growth by having the ability to produce IAA, dissolve phosphate, produce siderophores and fix nitrogen.
Cadangan Karbon di atas Permukaan Tanah pada Kawasan Cagar Alam Maninjau Blok Rehabilitasi Silayang-Malabur, Kabupaten Agam Novia Sherina A. Lubis; Irma Leilani Eka Putri
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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The Maninjau Nature Reserve is a conservation forest area located in the Agam district and Padang Pariaman district. In this case the Silayang-Malabur block is included in the rehabilitation block where this block has been damaged both naturally and due to human actions such as illegal logging, conversion of forests to fields and so on which has an impact on reducing carbon stocks. Therefore, it is necessary to do research in calculating carbon stocks to determine carbon stocks. This research was carried out in the Maninjau Nature Reserve area, Silayang-Malabur Rehabilitation Block, Agam Regency, which was conducted from December 2022 - January 2023. This type of research was descriptive research by collecting data on the level of trees, poles, saplings, and seedlings. Data collection used purposive random sampling which was divided into 2 research areas, namely natural forest and open/damaged forest. The sample plots used were a single plot of 3 plots randomly distributed in each study area. Biomass estimation using allometric equations. Based on the results of research on carbon stocks stored above ground level in the Maninjau Nature Reserve area of the Silayang-Malabur Rehabilitation Block, Agam Regency of 148,415 ton C/ha in natural forest areas and 34,397 ton C/ha in open/degraded forest area with a total carbon stock of 182,812 ton C/ha.
The potential of local orange peel-derived eco-enzymes in producing indole acetic acid Siska Alicia Farma; Nurfa Dewiza Luzik; Salma Sakina; Irma Leilani Eka Putri; Linda Advinda; Azwir Anhar
Acta Biochimica Indonesiana Vol. 6 No. 2 (2023): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.135

Abstract

Background: Ecoenzymes, created from the fermentation of organic citrus waste, offer a sustainable method to produce Indole Acetic Acid (IAA), a phytohormone vital for plant growth. This study investigates the potential of these ecoenzymes in promoting sustainable agriculture. Objective: This study aims to evaluate the capacity of ecoenzymes derived from local citrus organic waste to synthesize IAA hormones. Methods: The ecoenzyme was extracted from fruit powders and centrifuged to separate the supernatant. One ml of ecoenzyme supernatant was then mixed with 2 ml of Salkowski reagent and incubated for 12 hours at room temperature in the dark to facilitate reaction. The presence and concentration of IAA were determined using spectrophotometry at a wavelength of 530 nm, while total protein levels were measured using the Warburg-Christian method. Results: ecoenzymes from local citrus sources contain IAA, with the highest concentration observed in sample 7A (30.26 µg/ml). The ecoenzyme exhibited favorable characteristics, including an average degree of acidity of 3.55, and the highest total protein content was found in sample 2A (144.277 mg/mL). Conclusion: Ecoenzymes from local orange peels successfully produce IAA, supported by fermentation-induced microbial activity and acidic conditions. This highlights their potential in sustainable agriculture.
Co-Authors Achyar, Afifatul Al Adawiyah Alifya Putri Diva Atifah, Yusni Atiqahh Muthiah Azwir Anhar Des Des M Des M Dezi Handayani Dwi Hilda Putri Fadilla Sonia Putri Fevria, Resti Linda Advinda Mutia Sari Nia Faradila Novia Sherina A. Lubis Nurfa Dewiza Luzik Rahmawati Darussyamsu Ramadhan Sumarmin Relsas Yogica, Relsas S. Syamsurizal Salma Sakina Sartika Fazlina Silviana Okwisan Siska Alicia Farma Syifa Fajrisani Vauzia, Vauzia Violita Violita Yurico Utami