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ENDOPHYTIC TESTING OF Serratia marcescens strain NPKC3_2_21 AGAINST INPARA 3 RICE VARIETY Gunawan Sutio; Iskandar Iskandar; Lilik Tri Indriyati; Gunawan Djajakirana
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.372

Pest management is a crucial concern, especially when dealing with insect pests that can cause extensive damage to agricultural crops and the economy. One such pest is the rice stem borer (Scirpophaga innotata), which infests rice stems and poses a significant threat. To combat these pests, various microbial agents have been developed for effective pest control. Among these, the endophytic microbe in biological plant protection plays a key role in the program of sustainable pest management. An endophytic bacterium, Serratia marcescens strain NPKC3_2_21, has been identified as a key player in sustainable pest management, particularly in rice crops. In this study, we aimed to investigate the endophytic characteristics of S. marcescens strain NPKC3_2_21 in the swamp rice plant of the Inpara 3 variety of swamp rice plants. To establish its roles. Marcescens as an endophytic bacterium in rice plants, specifically the Inpara 3 variety, we conducted tests by inoculating bacteria on the plant tissue of rice plants that have been sterilized rice plant tissues with the bacteria. We assessed the presence of S. marcescens strain NPKC3_2_21 in plant tissues by applying isolates to the surface of rock wool, which supported the growth of wet rice plants aged ten or over ten days or older after planting. Samples were collected from the underside of the stem, the bottom of the leaves, and the roots on days 2, 7, and 14 after the application of isolates to the rock wool. The samples were then washed in 70% alcohol and 4% chlorox for 30 seconds and subsequently isolated on Luria Bertani (LB) agar media. Furthermore, we conducted tests to determine the ligninolytic, cellulolytic, and proteolytic activities of S. marcescens, which helped elucidate its endophytic ability. Based on the result, the endophytic capabilities. Based on the results, we found that S. marcescens strain NPKC3_2_21 exhibited endophytic characteristics solely in the stem tissue of the Inpara 3 rice variety. However, we did not observe its presence in the root and leaf tissues.

Harvesting Bioelectricity from Microbial Fuel Cells (MFCs) Powered by Electroactive Microbes Yohanna Anisa Indriyani; Iman Rusmana; Syaiful Anwar; Gunawan Djajakirana; Dwi Andreas Santosa
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 12, No 3 (2023): September 2023
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v12i3.583-596

The application of microbial fuel cells is still facing some challenges due to its low power output and high internal resistance. It is desirable to obtain a stable and consistent power output from an MFC to support practical real-world applications. Five electroactive bacteria (isolate LGf1, LGf11, LGf15, LGf20, and LGf22) isolated from the sediment of Waduk Saguling were exploited as the potential anodic biocatalyst for MFC, and the performance of these MFCs were studied in terms of voltage generation (open and close circuit), power density and the losses (polarization technique), and efficiencies (coulombic and energy). MFC biocatalyst by isolate LGf11 performed the best electrochemical performances, including highest OCV (open circuit voltage) value (804 mV) and power output (0.043 W/m2), lowest ohmic resistance (475 Ω), and highest coulombic efficiency (75.79%) and energy efficiency (88.36%) among all anodic biocatalysts. Nevertheless, all the five isolates were potential to be exploited as active biocatalyst for MFC due to their high OCV values and the stability of voltage generations, both in open circuit and close circuit mode. The development of system configuration and the use of more suitable substrate for different electroactive microbes in order to harvest more power output was recommended for further study. Utilization of these potential microbes for other applications in MFC (such as wastewater treatment etc.) was also suggested for further research. Keywords: Bio-electrochemical system, Biofuel, Efficiency, Electro-microbiology, Power output

Isolation and Electrochemical Evaluation of Electrogenic Bacteria from the Sediment of Two Waterlogged-Ecosystems Indriyani, Yohanna Anisa; Rusmana, Iman; Anwar, Syaiful; Djajakirana, Gunawan; Santosa, Dwi Andreas
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.9476

Electrogenic microbes have been exploited in the microbial fuel cell (MFC) system for harvesting bioelectricity. Electrogens are widely distributed in various environments, but the exploration of this microbial group from ecosystems in Indonesia is still limited. This present study aimed to isolate the electrogenic bacteria from sediments of two waterlogged ecosystems (dam and paddy field) using serial dilution into extinction method prior to streak plate method on the solid thioglycollate media supplemented with Fe3+. Electrochemical evaluation was conducted in glucose-fed dual-chamber MFC by using Arduino UNO-based data logger for the accurate monitoring of electricity production in the term of open circuit voltage (OCV). A total of 54 electrogens were successfully isolated from these two ecosystems, ranging from weakness to strongest electrogens (OCV >800 mV) and ranging from microaerophilic, aerotolerant and facultative anaerobes, to obligate anaerobes. This result also suggested that sediment of waterlogged ecosystems rich in electron donor and solid acceptor electron compounds could potentially host electrogenic microbes. The exploration of electrogens from many other waterlogged ecosystems in Indonesia, both natural and anthropic ecosystems, could be conducted to collect genetic resources of novel electrogenic bacteria for the development of MFC technology in Indonesia.

Slow- Release Fertilizer Formulation Using Acrylic and Chitosan Coating Handayani, Lili; Djajakirana, Gunawan; Darmawan, .; Munoz, Canecio Peralta
JOURNAL OF TROPICAL SOILS Vol. 20 No. 1: January 2015
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2015.v20i1.37-45

The low-efficiency problem in fertilizer application can be overcome by controlling fertilizer solubility, i.e. by rendering the fertilizer to be released gradually; such material is also known as slow-release fertilizer (SRF). This research was aimed to formulate SRF by coating technique using acrylic and chitosan as the coating material, and to evaluate fertilizer resistance to too fast disintegration, and rate of nutrient release method. The results demonstrated that fertilizer formulation containing N, P, K, Fe, Cu, and Zn with granulation technique yielded 74% of granules with 2-5 mm in diameter. The SRFs (formulated fertilizer with acrylic or chitosan coating) were more resistant to water pounding than non-SRF. Furthermore, shaking test with distilled water or 2% citric acid, or by percolation test with distilled water showed that the SRFs had lower nutrient solubility than the non-SRFs. The results of shaking test also specifically indicated that coating with acrylic made the fertilizer more resistant to the citric acid,suggesting that this coating material would be more suitable in acidic soils. The SRFs formulated with the addition of chitosan during blending of micronutrients prior to mixing with macronutrients, granulation, and final coating exhibited lower nutrient solubility than the SRFs without the pre-coating chitosan addition. [How to Cite: Lili H, G Djajakirana, Darmawan, and CP Munoz. 2015. Slow- Release Fertilizer Formulation Using Acrylic and Chitosan Coating. J Trop Soils 19: 37-45. Doi: 10.5400/jts.2015.20.1.37][Permalink/DOI: www.dx.doi.org/10.5400/jts.2015.20.1.37]

The Diversity and Abundance of Springtail (Collembola) on Forests and Smallholder in Jambi Widrializa, .; Widyastuti, Rahayu; Santosa, Dwi Andreas; Djajakirana, Gunawan
JOURNAL OF TROPICAL SOILS Vol. 20 No. 3: September 2015
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2015.v20i3.173-180

Harapan forests is the first restoration forest in Indonesia, includes several different ecosystems. Different ecosystems have different characteristic to affect the diversity and abundance of Collembola. In the ecosystem, Collembola have an important role in biocontrol, decomposition, soil nutrient distribution, stimulation of soil microbial activity and as an alternative feed for predators. This study was aimed to investigate the diversity and abundance of Collembola in four ecosystems at Harapan forest, i.e. secondary forest, rubber forest, rubber smallholder and oil palm smallholder. To achieve the objective, soil samples were taken at 48 observation points in the four ecosystem. The soil samples were then extracted by Kempson Extractor. The diversity and abundance of Collembola in four ecosystems were categorized as medium to high. The total of individual and population of Collembola in the secondary and rubber forest ecosystems were likely to be higher than in the rubber and oil palm smallholders. This study had also identified four orders, 14 families and 31 genera of Collembola, where Isotomiella and Proisotoma dominated the genus of Collembola in the four ecosystems. [How to Cite: Widrializa, R Widyastuti, DA Santosa and G Djajakirana. 2015. The Diversity and Abundance of Springtail (Collembola) on Forests and Smallholder in Jambi. J Trop Soils 20: 173-180. Doi: 10.5400/jts.2015.20.3.173]Â

Isolation and Characterization of Propoxur-Degrading Bacteria, Brucella pseudintermedia LED 6 from a Pineapple Plantation in Lampung Hidayat, Taufiq; Widyastuti, Rahayu; Djajakirana, Gunawan; Antonius, Sarjiya; Dewi, Tirta Kumala
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.725

Propoxur is a non-systemic carbamate insecticide that is widely used in Indonesia to control insect pests. However, its persistence and toxicity pose environmental concerns. Bioremediation with bacteria is a viable method for mitigating the detrimental effects of propoxur residues. The goal of this work was to isolate and characterize bacterial strains that can degrade propoxur. Isolation by enrichment culture procedures, utilizing propoxur as the sole carbon source. The isolates' morphological and physiological features were examined, and their degradation potential was determined. Six bacterial samples were isolated from a pineapple plantation in Lampung, Indonesia, and one strain, known as LED 6, showed great potential for propoxur breakdown. Molecular identification with 16S rRNA gene sequencing identified the isolate as Brucella pseudintermedia. Growth characterisation revealed that the isolate performed best at 28 °C and pH 7. After 72 hours of incubation with 500 ppm propoxur, LED 6 had deteriorated around 26% of the starting concentration. Keywords: bioremediation, Brucella pseudintermedia, pineapple, propoxur

Laju Dekomposisi dan Hara Tersedia dari Pangkasan Tumbuhan Bawah Perkebunan Kelapa Oktariani, Putri; Suryawijaya, Putu Kevin; Djajakirana, Gunawan; Iskandar
Jurnal Ilmu Tanah dan Lingkungan Vol 27 No 2 (2025): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.27.2.82-87

The implementation of organic farming in peatland coconut plantations faces challenges such as the low availability of natural nutrients, restrictions on the use of synthetic fertilizers and pesticides, and the demand for soil fertility conservation to ensure sustainable production. One potential solution is the utilization of undergrowth pruning, predominantly composed of Nephrolepis sp., as a source of organic nutrients.This study aimed to determine the decomposition rate and estimate the nutrient release potential from undergrowth pruning in a coconut plantation on peat soil in Riau Province, Indonesia. The litter bag method was employed with an observation period of eight weeks. Results showed that the decomposition rate of the pruned biomass reached 1.92 g per week, with a dry weight reduction of 68%. The most abundant nutrients released during decomposition were potassium (1.79%), phosphorus (0.07%), and copper (5.68 ppm). These findings suggest that undergrowth pruning has the potential to serve as an alternative nutrient source, supporting environmentally friendly coconut cultivation systems on peatlands.

Kelarutan Nikel Pada Campuran Tanah Berbahan Induk Ultrabasa dengan Gambut pada Kondisi Jenuh dan Tidak Jenuh Air Sumawinata, Basuki; Djajakirana, Gunawan; Simbolon, David Ricardo
Jurnal Ilmu Tanah dan Lingkungan Vol 25 No 1 (2023): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.25.1.25-29

Gambut tropik dengan bobot isi yang rendah berdampak pada berkurangnya kemampuan tanah gambut dalam menopang tanaman. Untuk meningkatkan bobot isi gambut maka dilakukan pencampuran tanah mineral dengan tanah gambut. Namun sumber tanah mineral yang dekat ke lahan gambut adalah tanah berbahan induk ultrabasa dengan kandungan logam Nikel (Ni) yang tinggi mencapai 5000 ppm. Karakter lahan gambut yang asam dan berada pada topografi yang rendah menjadi dugaan akan meningkatkan kelarutan Ni saat terjadi banjir. Oleh karena itu pencampuran bahan-bahan tanah dan perlakuan air bertujuan untuk mengukur kelarutan Ni pada kesetimbangan pH setelah pencampuran dan diekstrak menggunakan larutan NH4OAc 1.0 N pH 7.0; 4.8; 4.2, HCl 1.0 N; 0.1 N, dan H2SO4 1.0 N. Kesetimbangan pH setelah pencampuran bahan tanah berada pada pH 5.2 – 6.1 yang berarti kelarutan Ni ditunjukan oleh hasil ekstrak larutan NH4OAc 1.0 N pH 7.0 - 4.8. Pada rentang kesetimbangan pH tersebut nilai kelarutan Ni pada perlakuan penjenuhan (J) dan pengeringan (K) tidak berbeda nyata pada uji taraf 5%. Namun kelarutan Ni pada setiap penambahan bobot gambut berbeda nyata pada kondisi penjenuhan yang dikeringkan sampai kondisi lembab (JK).

Isolation and Electrochemical Evaluation of Electrogenic Bacteria from the Sediment of Two Waterlogged-Ecosystems Indriyani, Yohanna Anisa; Rusmana, Iman; Anwar, Syaiful; Djajakirana, Gunawan; Santosa, Dwi Andreas
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.9476

Electrogenic microbes have been exploited in the microbial fuel cell (MFC) system for harvesting bioelectricity. Electrogens are widely distributed in various environments, but the exploration of this microbial group from ecosystems in Indonesia is still limited. This present study aimed to isolate the electrogenic bacteria from sediments of two waterlogged ecosystems (dam and paddy field) using serial dilution into extinction method prior to streak plate method on the solid thioglycollate media supplemented with Fe3+. Electrochemical evaluation was conducted in glucose-fed dual-chamber MFC by using Arduino UNO-based data logger for the accurate monitoring of electricity production in the term of open circuit voltage (OCV). A total of 54 electrogens were successfully isolated from these two ecosystems, ranging from weakness to strongest electrogens (OCV >800 mV) and ranging from microaerophilic, aerotolerant and facultative anaerobes, to obligate anaerobes. This result also suggested that sediment of waterlogged ecosystems rich in electron donor and solid acceptor electron compounds could potentially host electrogenic microbes. The exploration of electrogens from many other waterlogged ecosystems in Indonesia, both natural and anthropic ecosystems, could be conducted to collect genetic resources of novel electrogenic bacteria for the development of MFC technology in Indonesia.

Fluks Metana dan Karakteristik Tanah pada Beberapa Macam Sistem Budidaya Suprihati, ,; Anas, Iswandi; Sabiham, Supiandi; Djajakirana, Gunawan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 34 No. 3 (2006): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Methane (CH4) is an important greenhouse gas that has a high heat trapping capacity thus potentially contribute to the global-warming. Agriculture is considered among the responsible emission sources of CH4. Relationship among soil characteristics, soil microbes, and CH4 flux is very important in understanding the mechanism involved in the mitigation effects of certain agriculture practices. Results of this research showed that rice field produced the highest CH4 flux (7.4976 ± 0.5299 mg CH4-C m-2 h-1, n=3), while vegetable, sweet potato, yam bean and corn cropping produced lower CH4 flux (-0.7708 ± 0.6434 to 0.4605 ± 0.5255 mg CH4-C m-2 h-1, n=3). Nitrifier population among cropping systems was 3.13x103 to 3.17x104 MPN g-1 soil (dry weight), while denitrifier population was 3.77x103 to 1.17x105 MPN g-1 soil (dry weight). There were no specific dominance proportion of nitrosomonas, nitrobacter, denitrifier and total propagule among cropping systems. The CH4 flux had highly correlation to soil water content (r = 0.951), soil pH (r = 0.852) and soil Eh (r = -0.982). Denitrifier had significantly correlation to soil pH (r = -0.635) and soil ammonium content (r = -0.681). Key words : CH4 flux, cropping system, soil characteristic, soil microbes

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