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All Journal Jurnal Dedikasi Jurnal Akuakultur Rawa Indonesia Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Acta Aquatica: Aquatic Sciences Journal Jurnal Riset Akuakultur Indonesian Journal of Tropical Aquatic Jurnal Abdi Insani Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Jurnal Perikanan

Anis Zubaidah

Universitas Muhammadiyah Malang

Author-ID : 2566239

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Public Health Other

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SALURAN PENCERNAAN UDANG JERBUNG, Penaeus merguiensis Diki Mulianto; Widyowati Mukti Widyowati; Hendra Raharja; Anis Zubaidah
Jurnal Riset Akuakultur Vol 16, No 4 (2021): (Desember, 2021)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Upaya untuk meningkatkan pemanfaatan protein dalam pakan dapat dilakukan dengan penambahan bakteri proteolitik. Penelitian ini bertujuan untuk skrining bakteri proteolitik pada saluran pencernaan udang Jerbung (Penaeus merguiensis) sebagai kandidat probotik. Skrining bakteri dilakukan dengan mengisolasi bakteri dari saluran pencernaan udang yang diperoleh dari tangkapan nelayan di pesisir pantai Pasuruan, Jawa Timur. Dari hasil isolasi diperoleh lebih dari 30 koloni yang tumbuh pada media, selanjutnya dipilih lima isolat yang dengan karakteristik morfologi yang berbeda. Lima isolat terpilih selanjutnya diuji aktivitas proteolitiknya pada media skim milk agar (SMA). Hasil penelitian menunjukkan aktivitas proteolitik terbesar pada isolat UD-1 dengan nilai aktivitas proteolitik sebesar 2,49 ± 0,9 cm; UD-2 sebesar 2,33± 0,24 cm; UD-3 sebesar 1,85 ± 0,07 cm; UD-4 sebesar 1,11 ± 0,43 cm; dan UD-5 sebesar 1,36 ± 0,07 cm. Tiga isolat dengan nilai aktivitas proteolitik tertinggi kemudian diuji pewarnaan gram, uji ketahanan terhadap kondisi asam (pH 3), uji penempelan bakteri, uji antagonistik terhadap bakteri Vibrio harveyi, uji patogenitas dan pengamatan pertumbuhan bakteri. Hasil uji pewarnaan gram dengan hasil gram positif pada ketiga isolat. Ketiga isolat mampu bertahan hidup pada kondisi asam (pH 3) selama delapan jam dengan nilai kepadatan (OD ë 620 nm) pada isolat tertinggi UD-1 (0,875). Uji antagonistic menunjukkan isolat UD-1, UD-2, dan UD-3 mampu menghambat pertumbuhan bakteri Vibrio harveyi dengan membentuk zona hambat di sekeliling isolat. Nilai antagonistik tertinggi pada isolat UD-1 sebesar 12,3 mm. Uji patogenitas yang dilakukan menunjukkan bahwa isolat tidak bersifat patogen pada udang budidaya. Berdasarkan hasil penelitian dapat disimpulkan bahwa isolat (UD-1, UD-2, dan UD3) yang diisolasi dari saluran pencernaan udang termasuk bakteri proteolitik dan memenuhi syarat sebagai bakteri kandidat probiotik.Efforts to increase the utilization of protein in feed can be made by adding proteolytic bacteria. This study aimed to screen proteolytic bacteria in the digestive tract of Banana shrimp (Penaeus merguiensis) as probiotic candidates. The bacterial screening was carried out by isolating bacteria from the digestive tract of shrimp obtained from fishermen’s catch on the coast of Pasuruan, East Java. From the isolation results, more than 30 colonies grew on the media, then five isolates were selected with different morphological characteristics. The five selected isolates were then tested for their proteolytic activity on Skim Milk Agar (SMA) media. The results showed the most excellent proteolytic activity in isolate UD-1 with a proteolytic activity value of 2.49 ± 0.9 cm; UD-2 is 2.33 ± 0.24 cm; UD-3 is 1.85 ± 0.07 cm; UD-4 is 1.11 ± 0.43 cm; and UD-5 is 1.36 ± 0.07 cm. The three isolates with the highest proteolytic activity values were tested for gram-positive staining, acid resistance test (pH 3), bacterial attachment test, antagonistic test against Vibrio harveyi bacteria, pathogenicity test, and bacterial growth observation. Gram stain test results with gram-positive results on the three isolates. The three isolates were able to survive in acidic conditions (pH 3) for eight hours with the highest density (OD ë 620 nm) value of UD-1 (0.875). The antagonistic test showed that isolates UD-1, UD-2, and UD-3 could inhibit the growth of Vibrio harveyi bacteria by forming an inhibitory zone around the isolates. The highest antagonistic value in the UD-1 isolate was 12.3 mm. The pathogenicity test carried out showed that the isolates were not pathogenic in cultured shrimp. Based on the study results, it can be concluded that the isolates (UD-1, UD-2, and UD-3) isolated from the shrimp’s digestive tract are proteolytic bacteria and qualify as probiotic candidate bacteria.

SCREENING BAKTERI SELULOLITIK DAN AMILOLITIK PADA RUMEN SAPI SEBAGAI KANDIDAT PROBIOTIK PADA BUDIDAYA IKAN SECARA IN VITRO Anis Zubaidah; Dony Prasetyo; Hany Handajani; Sulis Puji Rohmah; Dyah Ayu Puspita
Jurnal Riset Akuakultur Vol 14, No 4 (2019): (Desember, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Bakteri selulolitik dan amilolitik mampu mengubah selulosa dan amilum menjadi glukosa serta mampu menghasilkan enzim selulase dan amilase. Bakteri selulolitik dan amilolitik diisolasi dari rumen sapi. Tujuan dari penelitian ini adalah untuk mendapatkan bakteri selulolitik dan amilolitik dari rumen sapi yang memiliki nilai indeks selulolitik dan amilolitik yang tinggi, serta mampu memenuhi syarat untuk dijadikan sebagai probiotik. Screening bakteri pada rumen sapi menghasilkan enam isolat yaitu AR, BR, CR, DR, ER, dan FR. Uji aktivitas selulolitik dilakukan pada substrat carboxy methyl cellulose (CMC) dan amilolitik pada amilum, uji ketahanan terhadap kondisi asam (pH 3), pengamatan pertumbuhan bakteri selama 30 jam, uji antagonistik terhadap bakteri patogen Aeromonas hydrophila, uji penempelan bakteri dan uji patogenisitas bakteri pada ikan. Hasil penelitian menunjukkan bahwa aktivitas selulolitik terbesar pada isolat AR (2,67 ± 0,35 cm) dan aktivitas amilolitik terbesar pada isolat AR (4 ± 0,60 cm). Hanya empat isolat (AR, BR, ER, dan FR) yang dilakukan uji lanjut. Keempat isolat mampu bertahan dalam kondisi asam pH 3 selama delapan jam dengan nilai OD terbesar pada isolat ER (1.137). Uji antagonistik menunjukkan bahwa isolat AR, ER, dan FR mampu menghambat pertumbuhan bakteri Aeromonas hydrophila. Isolat FR memiliki nilai antagonistik terbesar yang ditandai dengan terbentuknya zona hambat 17 mm. Uji penempelan bakteri untuk membuktikan bahwa isolat mampu menempel pada usus ikan. Uji patogenesitas pada isolat yang didapatkan guna membuktikan bahwa isolat tidak bersifat patogen terhadap inang. Berdasarkan hasil penelitian dapat disimpulkan bahwa isolat yang didapatkan dari rumen sapi termasuk bakteri selulolitik dan amilolitik yang telah memenuhi syarat sebagai kandidat probiotik pada ikan.Cellulolytic and amylolytic bacteria can transform cellulose and starch into glucose and produce the cellulase and amylase enzymes. These types of bacteria can be found in and isolated from cow’s rumen. Thus, the purpose of this study was to obtain potential cellulolytic and amylolytic bacteria from cow’s rumen with a high cellulolytic and amylolytic index value and can be qualified as probiotics. The screening of bacteria in the cow’s rumen produces six isolates i.e. AR, BR, CR, DR, ER, and FR. The parameters observed were: the cellulolytic activity in carboxymethyl cellulose (CMC) substrate, amylolytic activity on starch substrates, resistance test to acidic conditions (pH 3), bacterial growth for 30 hours, antagonistic to pathogenicity test to bacteria Aeromonas hydrophila, bacterial attachment test, and bacterial pathogenicity test in fish. The results showed that the largest cellulolytic and amylolytic activities were observed in AR isolates with a value of 2.67 ± 0.35 cm) and 4 ± 0.60 cm, respectively. Only four isolates (AR, BR, ER, and FR) were used in further tests. The four isolates were able to survive in the acidic conditions of pH 3 for 8 hours with the largest (OD) value was achieved by ER isolates (1,137). The growth of each isolate was different. The antagonistic test showed that the three isolates could inhibit the growth of A. hydrophila. FR isolates had the greatest antagonistic values characterized by the formation of an inhibition zone of 17 mm. Bacteria attachment test proved that the isolates were able to stick in the fish gut. The pathogenicity tests also proved that the isolates were not pathogenic to the host.

Effectiveness of strychnine bush extract (Strychnos ligustrina blume) against to prevalence and survival rate of Dumbo catfish (Clarias gariepinus) infected with Aeromonas hydrophila Anis Zubaidah; Sri Samsundari; Hidayaturrahmi Hidayaturrahmi
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 2 No. 1 (2019): February
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v2i1.5601

One of the problemsofintensive catfish culture is the attack of Motile Aeromonas Septicemia disease caused by Aeromonas hydrophila bacteria. To prevent it is necessary to prevent the use of natural antibiotics that is strychnine bush extract. The purpose of this research is to know the ability of strychnine bush extract (Strychnos ligustrina Blume) using methanol solvent in inhibiting the growth of Aeromonas hydrophila bacteria and its effect on prevalence and survival rate in Dumbo catfish (Clarias gariepinus). The method used in this research is the exsperimen method by using complete randomized design (RAL). In this study there were five treatments with different doses of treatment (P1: 6%), (P2: 12%), (P3: 18%), (P4: 24%) and (P5: 30%), each treatment was repeated three replications. Furthermore, the data were analyzed using ANAVA and continued with LSD test. The results showed that the giving of strychnine bush extract with different concentrations had a very significant effect on the width of the Aeromonas hydrophila bacterial resistance zone. The lowest inhibition of the treatment (P1: 6%) with a resistance of 1.79 mm. While the highest inhibition of treatment (P5: 30%) with 8.29 mm inhibition power. In prevalenceofdumbo catfish with givingofsongga extract very real effect, treatment with lowest prevalence is treatment (P4: 24%) with prevalence value 23,3% andhighestpervalence that is treatment (P5: 30%) with prevalence value equal to 40 %. In the synthesisofdumbo catfish with the giving of strychnine bush extract have a very real effect, the treatment with lowest stability is treatment (P4: 30%) with 77% syntax value and the highest synthesis of treatment (P5: 24%) with 90% syntax value. This study resulted in the conclusion that the best treatment for prevalence and survival rate is treatment (P4: 24%). As for the higher inhibition power dose strychnine bushextractis used then the higher the power inhibition.

EVALUATION FEASIBILITY AND BUSINESS DEVELOPMENT STRATEGY ENLARGEMENT WHITE SHRIMP (Litopenaeus Vannamei) AT DUKUHTUNGGAL VILLAGE GLAGAH SUB DISTRICT LAMONGAN Ika Maulana Lutfi; David Hermawan; Anis Zubaidah
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 1 No. 1 (2018): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v1i1.6077

This research aims to analyze the financial feasibility and formulating business development strategies enlargement of White shrimp at Dukuhtunggal Village Sub district Glagah Lamongan. The research using survey method. The sampling method used snowball sampling by the number of respondents as many as 18 people. The result showed that the business of enlarging white shrimp enlargement enterprise development deserves to be implemented and developed, because of the results of the assessment in accordance with the investment criteria Net Present Value (NPV) reached Rp 283.927.334, Net Benefit Cost Ratio (Net/B/C) by 28, Internal Rate of Return (IRR) reached 645.5%, the value of payback period of 0.37 or three months of seven days and the Profitability index (PI) of 1.53. Business development strategy is known through proceeds from the matrix IFE and EFE with a total score of 3.341 and 3.272. Matrix IE shows the position of the company located on the cell to one which means grow and build so that the appropriate strategy to be applied is the intensive strategy i.e., market penetration, market development, product development and integration strategies. The formulation of the strategy produces eight alternative development strategies i.e., an increase in production capacity, strengthening the institutional role, leveraging capital access, empowerment of farmers, increase the use of quality seeds, weaves cooperation with suppliers, maintaining the condition of the infrastructure, and improve the implementation and monitoring of standard operational procedures. Keywords: Litopenaeus vannamei, feasibility, strategy enlargement

Effectiveness of Strychnos ligustrina Bl. Extract as Feed Supplementation to Increase Immune System of Nile Tilapia (Oreochromis niloticus) wich againts Streptococcus agalactiae Anis Zubaidah; Kuny Riski Faidah; Sri Samsundari
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 1 No. 1 (2018): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v1i1.6082

One of the problems of intensive cultivation of tilapia is the attack of Streptococcosis disease caused by Streptococcus agalactiae bacteria. To prevent it is necessary to prevent the use of natural antibiotics that is Strychnine bush extract. The method used in this research is the experimental method using Completely Randomized Design (RAL), then the data is analyzed by using ANAVA. In this research, there were five treatments with various dosages, namely treatment (K: 0%), (A: 5%), (B: 10%), (C: 15%) and (D: 20%), each treatment was repeated four times. The main parameters used are hematocrit level, leukocrit level, leukocyte count and phagocytosis activity. As a supporting parameter is water quality and MIC (Minimum Inhibitation Concentration) test. The results showed that dosage of Strychnine bush extract did not affect the level of hematocrit and leukocrit level, but significant effect on leukocyte count with the highest value at treatment B with 10% dose is 11,9 × 105 cell / mm3. Giving extract also significant effect on phagocytosis activity with the highest phagocytosis value at treatment B with dose of 10% that is 32%.Keywords: Imune system, Streptococcosis, antibiotics, Minimum Inhibitation Concentration

Growth performance of Wader Cakul juvenile (Puntius binotatus) with different stocking density Dony Prasetyo; Anis Zubaidah; Vera Noviana
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 2 No. 1 (2019): February
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v2i1.10965

One of the freshwater fishes thatbegan to bedeveloped is Wader fish, but there is still a lot of information that has notbeen supportedon fish farming techniques. One of them is the optimal stocking density for cultivation. The purpose of this study was to study the effects of different stocking densities on the growth and survival rates of the WaderCakulfish (Puntiusbinotatus).This research was conductedin May - July 2018. The method used in this study was to use aCompletely Randomized Design with 4 training, namely P1 (2 fish/liter), P2 (3 fish/liter), P3 (4 fish/liter) and P4 (5 fish/liter).Each treatment was repeated3 times. The main parameters were absolute growth rate (length and weight gain)(GR), specific growth rate (SGR), survival rate (SR), and feed conversion ratio (FCR).Data were analyzedusing ANOVA (analysis of variance) and continued with the Least Significant Difference Test. The results showed that different stocking densities gave significant values (P <0.05) for GR, SGR, SR and FCR comparisons. The best treatment in this study was P1 with a growth value of 0.31 ± 0.01 gram, SGR 3.50 ± 0.03%, Long Growth 1.97 ± 0.05 cm, SR 85.07 ± 2.17% and FCR 1.47 ± 0.02. P1 (2 fish/liter) is the best maintenance to increase the growth, survival rate, and feed efficiency of thecakulwader fish.

Effectiveness of extract Songga wood (Strychnos lucida) towards survival and antibody titers on Tilapia (Oreocromis niloticus) that infected Streptococcus agalactiae Anis Zubaidah; Sri Samsundari; Vivi Fitriani
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 2 No. 2 (2019): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v2i2.11136

Nile Tilapia or Tilapia Fish (Oreochromis niloticus) is one of commodity with a high number of consumers. But, Streptococcus agalactiae attack caused a decline in production. One way to reduce these diseases by using natural materials of songga wood (Strychnos lucida R.Br) where the songga wood contains many compounds like alkaloid, phenols, flavonoids, triterpenoids, which can inhibit bacterial growth by damaged cell membrane. The results showed that the inhibitory songga wood extract against Streptococcus agalactiae was highly significant, and shows with the highest survival rate of 90% and proved by theincreasingantibodytitersafteraddingsonggawood extract.

Trend analysis of catching fish Decapterus sp. in the Muncar Coastal Fisheries Port, Banyuwangi, Indonesia Devi Purwinda; Hany Handajani; Anis Zubaidah
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 3 No. 2 (2020): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v3i2.12939

Small pelagic fish resources in the Java Sea, especially Banyuwangi, are dominated by catches of Decapterus sp, which are mostly landed at the UPT of the Coastal Fisheries Port Muncar. This study aimed to provide information on CPUE trends (Catch Per Unit Effort) and the seasonality of flying fish in the Coastal Fisheries Port Muncar as an effort to encourage effective and sustainable in using of fishery resources. The method used in this study was a survey. The research data was obtained through secondary data including time series of the production of catches of flying fish, and trip data of purse seine, payang and bagan from 2015 to 2019 collected by Coastal Fisheries Port Muncar. The analysis used the CPUE analysis by comparing catches to capture attempts. The results showed, from 2015 to 2019, the highest CPUE of flying fish occurred in 2019, amounting to 13 365.5 kg/trip, while the lowest CPUE occurred in 2016 amounted to 1 050.6 kg/trip. The trend of CPUE flying fish from 2015 to 2019 experienced a fluctuating increase with an average of 7 327.5 kg/trip. A good season for flying fishing occurs from May to December, and shares a peak in December, while famine occurs in January.

Distribution of Macrozoobentos In The Estuary of Seletreng River, Situbondo District Lukman Al Hakim; Hariyadi Hariyadi; Anis Zubaidah
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 3 No. 1 (2020): February
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v3i1.13253

The estuary of the Seletreng river is located in the Banyuglugur area of the Situbondo Regency and is directly adjacent to the shrimp pond area. The estuary of this river is widely used by local residents to look for fish, crabs. The existence of shrimp ponds near the mouth of this river has made many changes to the surrounding ecosystem. The purpose of this study is to determine the diversity index of macrozoobenthos and to determine the uniformity of macrozoobenthos. This research was conducted in February-March 2019, located in the waters of the mouth of the Seletreng Banyuglugur river, Situbondo district. Uses 5 stations with 3 repetitions. The diversity index value at the five stations, namely at station 1 is at a value of 0.66 which is categorized as low, at station 2 it is 0.36, at station 3 is 0.53, at station 4 is 0.65, at station 5 is 0, 25 wherefrom all stations the diversity index value is less than 1 and categorized as low diversity. The uniformity index value at the five stations, namely at station 1 is at a value of 0.37, at station 2 is 0.18, at station 3 is 0.24, at station 4 is 0.40, at station 5 is 0.12 wherefrom all stations diversity index value less than 1 and categorized on uniformity depressed.

The effectiveness of cellulolytic bacteria on feeds towards the immune response of Tilapia Dony Prasetyo; Anis Zubaidah; Hany Handajani; Emilio Hudin
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 4 No. 2 (2021): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v4i2.20360

Tilapia is one of fishery commodities that is very good to be developed in thecultivation sector. However, the tilapia often incurs with the diseases so thatthe cultivators have difficulty in maintaining the health of the tilapia. One ofthe ways to improve the health is by distributing the cellulolytic bacteriaaddition and blood is one of parameters that used to identify the tilapia’shealth. The research method used was an experimental quantitative methodby preserving the fish and giving cellulolytic bacteria on the feeds for 30 days;then, the bloods ware taken to test the immune system. The parameters usedwere 12 parameters, of which parameters involved of 8 main parameters andsupporting parameters. The conclusion of this research is that the besttreatments are in the treatments of P1 because 4 main parameters out of 8main parameters experience an increasement and 6 main parametersexperience highest increasement from all of the treatments. For the P3, thehighest increasement of main immune system is in the leukocytes andphagocytosis activity. Thus, the fish can prevent the outside attacks.

Co-Authors Ahmad Uzair Baihaqi Alfi Maulina Nurhuda Alif Zidane Juni Wananda Arianto, Doddy David Hermawan Desi Indah Ramadani Dessy Amitha Kusumaningdyah Devi Purwinda Diki Mulianto Dimas Calvin Dony Prasetyo Dyah Ayu Puspita Emilio Hudin Faidah, Kuny Riski Fia Sulis Setiani Ganjar Adhywirawan Sutarjo Hany Handajani HARI PURNOMO Hariyadi Hariyadi Hariyadi Hariyadi, Hariyadi Hendra Raharja Hermawan, David Hidayaturrahmi Hidayaturrahmi Ika Maulana Lutfi Insan, Yudi Arifraeka Kamila, Dina Izzah Kuny Riski Faidah Lasmi, Huda Lukman Al Hakim Lutfi, Ika Maulana Masitoh Masitoh Muhammad Abdul Qodir Jaelani Mujiono Nailin, Nila Choirun Noviana, Vera Nur Ocvanny Amir Nurhuda, Alfi Maulina Olivia Agustin Olivia Agustin Raharja, Hendra Ridho Firmandhaka Riza Rahman Hakim Sari, Yussandra Khartika Seviana, Niken Laili Siti Fatimah Soni Andriawan Sri Dwi Hastuti Sri Samsundari Sri Samsundari Sri Samsundari Sri Samsundari Sri Samsundari Sri Samsundari Sri Samsundari, Sri Sulis Puji Rohmah Titan Aji Wiratama Vera Noviana Vivi Fitriani Widyowati Mukti Widyowati Yayang Dita Wulandari Yudi Arifraeka Insan

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