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Anis Zubaidah
Universitas Muhammadiyah Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Public Health Other

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Optimizing the productivity of vaname shrimp fry (Litopenaeus vannamei) through giving Potassium Nitrate (KNO3) to the cultivation of Skeletonema sp Handajani, Hany; Zubaidah, Anis; Nailin, Nila Choirun; Purnomo, Hari
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica, Vol. 12: No. 2 (August, 2025)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v12i2.21451

Abstract

Skeletonema sp. culture its growth is strongly influenced by the nutrients in the environment where it lives, therefore fertilizer is needed in the culture media to support the availability of both macro and micro nutrients. The purpose of this study was to determine the effect of KNO3 administration on the growth and density of Skeletonema sp. cells. and dosage to obtain the best KNO3 fertilizer. This study used a completely randomized design (CRD), consisting of 4 treatments and 3 replications. KNO3 fertilizer dosage treatments included A (100 ppm), B (200 ppm), C (300 ppm), and control K (500 ppm). Parameters measured were specific growth rate and cell density of Skeletonema sp. The results of the study on density showed that the best treatment was treatment K of 269.5 ᵡ 104 cells/mL, followed by C 300 ppm (203.5 ᵡ 104 cells/mL), B 200 ppm (126.08 ᵡ 104 cells/mL), and A 100 ppm (94.75 ᵡ 104 cells/mL). The average specific growth rate produced in each treatment corresponds to the cell density pattern, namely K (0.434/day); C(0.379/day); B(0.279/day); and A (0.220/day). The application of KNO3 fertilizer with different doses had a significant effect on the growth and density of Skeletonema sp. cells. The higher the KNO3 dose, the higher the growth rate and density of Skeletonema sp cells. high too. Keywords: Fertilizer Dose; Kalium Nitrate; Phytoplankton
RESPONS KEKEBALAN BAWAAN IKAN NILA (Oreochromis niloticus) YANG DIBERI PAKAN DENGAN SUPLEMENTASI DAUN ALPUKAT (Parsea americana Mill) Hastuti, Sri Dwi; Zubaidah, Anis; Fatimah, Siti
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.15-29

Abstract

Penyakit pada budidaya ikan nila merupakan permasalahan utama yang dapat menurunkan produksi dan menyebabkan kerugian. Selama ini upaya pengobatan dan pencegahan penyakit dilakukan dengan menggunakan antibiotik dan bahan kimia yang tidak ramah lingkungan, sehingga dapat meninggalkan residu pada daging ikan dan mencemari perairan. Oleh karena itu perlu alternatif penanggulangan penyakit dengan pemanfaatan bahan alami seperti daun alpukat yang mengandung senyawa saponin, tanin, flavonoid, alkaloid, dan fenol yang dapat berfungsi sebagai imunostimulan. Penelitian ini bertujuan untuk mengevaluasi respons kekebalan nonspesifik ikan nila yang diberi pakan dengan suplementasi daun alpukat. Metode yang digunakan adalah eksperimen dengan rancangan acak lengkap menggunakan empat perlakuan dan tiga ulangan. Dosis perlakuan yang digunakan adalah: perlakuan P1 (suplementasi daun alpukat 0,25%); perlakuan P2 (suplementasi daun alpukat 0,5%); perlakuan P3 (suplementasi daun alpukat 0,75%); dan perlakuan P0 (kontrol tanpa suplementasi daun alpukat). Hasil penelitian menunjukkan bahwa ikan nila yang diberi pakan mengandung daun alpukat menunjukkan level hematokrit, leukokrit, dan aktivitas fagositosis yang berbeda nyata (P<0,05) dengan ikan yang diberi pakan kontrol. Hematokrit tertinggi diperoleh pada dosis  0,5%;  sedangkan leukokrit tertinggi diperoleh pada dosis 0,75% dan aktivitas fagositosis terbaik diperoleh pada dosis 0,75% dengan nilai sebesar 33,69, 2,44, dan 72% secara berturut-turut. Suplementasi daun alpukat pada pakan tidak berpengaruh nyata terhadap pertumbuhan dan kelangsungan hidup ikan nila. Dari hasil penelitian dapat disimpulkan bahwa daun alpukat memiliki potensi dalam meningkatkan sistem kekebalan bawaan ikan nila, khususnya pada hematokrit dan leukokrit serta aktivitas fagositosisnya.Disease in tilapia farming is a major problem that can reduce production and inflict irrevocable economic losses. Conventionally, efforts to treat and prevent diseases are carried out using antibiotics and chemicals that are not environmentally friendly, leaving residues on the fish flesh and polluting the aquatic environment. Therefore, alternative disease preventions and cures are increasingly researched, focusing on natural ingredients such as avocado leaves, which contain saponins, tannins, flavonoids, alkaloids, and phenols that can function as immunostimulants. This study aimed to evaluate the nonspecific immune response of tilapia fed with feed supplemented with avocado leaves. The study used experimental units consisting of four treatments and three replications arranged in a completely randomized design. The treatment doses were: treatment P1 (0.25% avocado leaves supplementation); treatment P2 (0.5% avocado leaves supplementation); treatment P3 (0.75% avocado leaves supplementation); and treatment P0 (control without avocado leaves supplementation). The results showed that tilapia fed with feed containing avocado leaves showed levels of hematocrit, leukocrit, and phagocytic activity that were significantly different (P<0.05) from the fish fed with the control feed. The highest hematocrit was obtained at the dose of 0.5%; while the highest leukocrit was obtained at the dose of 0.75% and the best phagocytic activity was obtained at the dose of 0.75% with values of 33.69, 2.44, and 72%, respectively. Supplementation of avocado leaves in feed did not have a significant effect on the growth and survival of tilapia. Based on the results, This study concludes that avocado leaves have the potential to improve the innate immune system of tilapia, especially in hematocrit and leukocrit as well as phagocytic activity.
EFEKTIVITAS PEMBERIAN PROBIOTIK YANG BERBEDA TERHADAP RESPON IMUN IKAN LELE SANGKURIANG (Clarias gariepinus) PADA BUDIDAYA SISTEM INTENSIF Seviana, Niken Laili; Zubaidah, Anis; Hastuti, Sri Dwi
Jurnal Riset Akuakultur Vol 17, No 3 (2022): (September) 2022
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.17.3.2022.191-203

Abstract

Lele sangkuriang (Clarias gariepinus) adalah spesies budidaya yang digemari masyarakat Indonesia dan memiliki potensi besar untuk dikembangkan. Budidaya ikan lele secara intensif yang saat ini banyak dikembangkan memiliki faktor resiko munculnya penyakit. Penyakit ini dapat menghambat keberhasilan budidaya lele, salah satu penyakitnya yaitu disebabkan oleh bakteri. Penyakit bakteri pada kegiatan budidaya ikan sudah menjadi masalah yang sering dihadapi pembudidaya. Salah satu jenis bakteri yang menyebabkan penyakit pada ikan lele sangkuriang (Clarias gariepinus) adalah bakteri Aeromonas hydrophilla. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas pemberian probiotik yang berbeda terhadap respon imun ikan lele sangkuriang pada budidaya sistem intensif. Penelitian ini dilaksanakan di Laboratorium Perikanan Fakultas Pertanian dan Peternakan Universitas Muhammadiyah Malang selama 40 hari.  Ikan lele sangkuriang diberikan perlakuan perbedaan probiotik yang dicampur pada pakan untuk memaksimalkan efektivitas terhadap imunitas ikan lele sangkuriang. Metode yang digunakan adalah rancangnan acak lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan P1 (kontrol) tanpa menggunakan probiotik, perlakuan P2 menggunakan probiotik dengan merk EM4, perlakuan P3 dengan merk probiotik Raja Lele dan perlakuan P4 dengan merk probiotik Minaraya. Parameter yang diamati antara lain survival rate, total eritrosit, total leukosit, hematokrit, aktifitas fagositosis dan kualitas air yaitu suhu, pH dan DO. Hasil data dianalisis ANOVA menggunakan software Excel, didapatkan hasil berbeda nyata. Hasil tertinggi pada setiap parameter antara lain, parameter SR perlakuan P3 dengan nilai 85,73±0,36%, total eritrosit perlakuan P3 dengan nilai 273 104 sel/mm3, total leukosit perlakuan P3 110,16  103 sel/mm3, hematokrit perlakuan P3 dengan nilai 29,9±0,91%, AF perlakuan P3 dengan nilai 66±1,63%. Berdasarkan hasil penelitian dapat disimpulkan bahwa probiotik yang memberikan respon imun terbaik adalah pada perlakuan P3 (Raja Lele).
EFIKASI VAKSIN Aeromonas hydrophila TERHADAP IMUNITAS IKAN LELE SANGKURIANG (Clarias gariepinus) DENGAN METODE INFILTRASI HIPEROSMOTIK Zubaidah, Anis; Sari, Yussandra Khartika; Hastuti, Sri Dwi; Handajani, Hany
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.31-44

Abstract

Kendala yang sering dialami pembudidaya ikan lele salah satunya yaitu serangan motile Aeromonas septicemia (MAS). Vaksinasi melalui perendaman merupakan cara yang efektif untuk meningkatkan sistem imun pada tubuh ikan lele, namun kurang memberikan hasil yang optimal sehingga perlu adanya penambahan metode infiltrasi hiperosmotik untuk memaksimalkan penyerapan vaksin. Penelitian ini bertujuan untuk menentukan kisaran salinitas yang baik dalam memaksimalkan penyerapan vaksin Aeromonas hydrophila pada ikan lele. Penelitian ini menggunakan metode eksperimental rancangan acak lengkap dengan lima taraf perlakuan dan tiga kali ulangan, antara lain kontrol negatif (Kn), kontrol positif (Kp), perendaman salinitas 3 ppt (P1), perendaman salinitas 6 ppt (P2), dan perendaman salinitas 9 ppt (P3) pada ikan lele berukuran 12-15 cm. Parameter yang diamati antara lain titer antibodi, relative percent survival, survival rate (SR), total eritrosit, total leukosit, kualitas air, dan gejala klinis. Hasil penelitian menunjukkan nilai tertinggi yaitu pada P2 (6 ppt) dengan nilai titer antibodi sebesar 8,0 ± 0,0, relative percent survival 100%, survival rate 100%, dan total eritrosit 2,80 x 106 sel mm-3, namun total leukosit pada P2 (6 ppt) menunjukkan nilai terendah karena leukosit melawan serangan patogen sehingga jumlah sel menurun. Disimpulkan bahwa perendaman dalam salinitas 6 ppt merupakan salinitas terbaik pada ikan lele dan berbeda nyata (P<0,05) dengan perlakuan lainnya karena proses penyerapan vaksin terjadi secara maksimal sehingga dapat meningkatkan sistem imun ikan lele.One of the obstacles often experienced by catfish farmers is attacks by the motile Aeromonas septicemia (MAS). Vaccination through immersion is an effective way to improve the immune system in the body of catfish, but it does not provide optimal results so it is necessary to add a hyperosmotic infiltration method to maximize vaccine absorption. This study aimed to determine an optimum salinity range to maximize the absorption of the Aeromonas hydrophila vaccine in catfish. This study used a completely randomized design experimental method with five treatment levels and three replications, including negative control (Kn), positive control (Kp), 3 ppt salinity immersion (P1), 6 ppt salinity immersion (P2), and 9 ppt salinity immersion (P3) in catfish sizing 12-15 cm. The parameters observed included antibody titer, relative percent survival, survival rate (SR), total erythrocytes, total leukocytes, water quality, and clinical symptoms. The results of the study showed that the highest value was at P2 (6 ppt) with an antibody titer value of 8.0 ± 0.0, relative percent survival 100%, survival rate 100%, and total erythrocytes 2.80 x 106 cells mm-3, while total leukocytes in P2 (6 ppt) showed the lowest value because leukocytes fought against pathogen attacks so that the number of cells decreased. It was concluded that immersion in 6 ppt salinity was the best salinity for catfish and was significantly different (P<0.05) from other treatments because the vaccine absorption process occurred optimally so that it could improve the catfish's immune system.
PENDAMPINGAN BUDIDAYA IKAN KOI SISTEM KOLAM AIR DERAS DI WISATA BOONPRING KABUPATEN MALANG, JAWA TIMUR Zubaidah, Anis; Hariyadi
Jurnal Abdi Insani Vol 11 No 3 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i3.1834

Abstract

The development of swift water cultivation systems provides various advantages, including high dissolved oxygen, sufficient natural feed, high stocking density, and lower production costs. This technology can be developed among fish farming groups, especially those who have access to extensive water sources. The implementation of this community service program aims to distribute koi fish cultivation technology using a fast water system to the community, especially in the Boonpring tourist area, Sanankerto Village, Malang Regency. The method used is to provide assistance to partners in intensive koi fish cultivation technology using a fast water pond system. The results obtained are the implementation of community service activities carried out by the activity implementation team at the Boon Pring Tourism Village, as well as increasing public understanding regarding optimizing the management of koi fish cultivation land using a fast water system. The survey results show that there has been a 30% increase in public understanding regarding fast water system koi fish cultivation. The conclusion of this activity is that the technology for cultivating koi fish using a fast water system has been successfully implemented at the Boonpring tourist attraction, Sanankerto Village, Malang Regency.
Co-Authors Ahmad Uzair Baihaqi Alfi Maulina Nurhuda Alif Zidane Juni Wananda Arianto, Doddy David Hermawan Desi Indah Ramadani Dessy Amitha Kusumaningdyah Devi Purwinda Diki Mulianto Dimas Calvin Dony Prasetyo Dyah Ayu Puspita Emilio Hudin Faidah, Kuny Riski Fia Sulis Setiani Ganjar Adhywirawan Sutarjo Hany Handajani HARI PURNOMO Hariyadi Hariyadi Hariyadi Hariyadi, Hariyadi Hendra Raharja Hermawan, David Hidayaturrahmi Hidayaturrahmi Ika Maulana Lutfi Insan, Yudi Arifraeka Kamila, Dina Izzah Kuny Riski Faidah Lasmi, Huda Lukman Al Hakim Lutfi, Ika Maulana Masitoh Masitoh Muhammad Abdul Qodir Jaelani Mujiono Nailin, Nila Choirun Noviana, Vera Nur Ocvanny Amir Nurhuda, Alfi Maulina Olivia Agustin Olivia Agustin Raharja, Hendra Ridho Firmandhaka Riza Rahman Hakim Sari, Yussandra Khartika Seviana, Niken Laili Siti Fatimah Soni Andriawan Sri Dwi Hastuti Sri Samsundari Sri Samsundari Sri Samsundari Sri Samsundari Sri Samsundari Sri Samsundari Sri Samsundari, Sri Sulis Puji Rohmah Titan Aji Wiratama Vera Noviana Vivi Fitriani Widyowati Mukti Widyowati Yayang Dita Wulandari Yudi Arifraeka Insan