Garuda - Garba Rujukan Digital

EFIKASI VAKSIN Aeromonas hydrophila TERHADAP IMUNITAS IKAN LELE SANGKURIANG (Clarias gariepinus) DENGAN METODE INFILTRASI HIPEROSMOTIK Zubaidah, Anis; Sari, Yussandra Khartika; Hastuti, Sri Dwi; Handajani, Hany
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.31-44

Kendala yang sering dialami pembudidaya ikan lele salah satunya yaitu serangan motile Aeromonas septicemia (MAS). Vaksinasi melalui perendaman merupakan cara yang efektif untuk meningkatkan sistem imun pada tubuh ikan lele, namun kurang memberikan hasil yang optimal sehingga perlu adanya penambahan metode infiltrasi hiperosmotik untuk memaksimalkan penyerapan vaksin. Penelitian ini bertujuan untuk menentukan kisaran salinitas yang baik dalam memaksimalkan penyerapan vaksin Aeromonas hydrophila pada ikan lele. Penelitian ini menggunakan metode eksperimental rancangan acak lengkap dengan lima taraf perlakuan dan tiga kali ulangan, antara lain kontrol negatif (Kn), kontrol positif (Kp), perendaman salinitas 3 ppt (P1), perendaman salinitas 6 ppt (P2), dan perendaman salinitas 9 ppt (P3) pada ikan lele berukuran 12-15 cm. Parameter yang diamati antara lain titer antibodi, relative percent survival, survival rate (SR), total eritrosit, total leukosit, kualitas air, dan gejala klinis. Hasil penelitian menunjukkan nilai tertinggi yaitu pada P2 (6 ppt) dengan nilai titer antibodi sebesar 8,0 ± 0,0, relative percent survival 100%, survival rate 100%, dan total eritrosit 2,80 x 106 sel mm-3, namun total leukosit pada P2 (6 ppt) menunjukkan nilai terendah karena leukosit melawan serangan patogen sehingga jumlah sel menurun. Disimpulkan bahwa perendaman dalam salinitas 6 ppt merupakan salinitas terbaik pada ikan lele dan berbeda nyata (P<0,05) dengan perlakuan lainnya karena proses penyerapan vaksin terjadi secara maksimal sehingga dapat meningkatkan sistem imun ikan lele.One of the obstacles often experienced by catfish farmers is attacks by the motile Aeromonas septicemia (MAS). Vaccination through immersion is an effective way to improve the immune system in the body of catfish, but it does not provide optimal results so it is necessary to add a hyperosmotic infiltration method to maximize vaccine absorption. This study aimed to determine an optimum salinity range to maximize the absorption of the Aeromonas hydrophila vaccine in catfish. This study used a completely randomized design experimental method with five treatment levels and three replications, including negative control (Kn), positive control (Kp), 3 ppt salinity immersion (P1), 6 ppt salinity immersion (P2), and 9 ppt salinity immersion (P3) in catfish sizing 12-15 cm. The parameters observed included antibody titer, relative percent survival, survival rate (SR), total erythrocytes, total leukocytes, water quality, and clinical symptoms. The results of the study showed that the highest value was at P2 (6 ppt) with an antibody titer value of 8.0 ± 0.0, relative percent survival 100%, survival rate 100%, and total erythrocytes 2.80 x 106 cells mm-3, while total leukocytes in P2 (6 ppt) showed the lowest value because leukocytes fought against pathogen attacks so that the number of cells decreased. It was concluded that immersion in 6 ppt salinity was the best salinity for catfish and was significantly different (P<0.05) from other treatments because the vaccine absorption process occurred optimally so that it could improve the catfish's immune system.

PENDAMPINGAN BUDIDAYA IKAN KOI SISTEM KOLAM AIR DERAS DI WISATA BOONPRING KABUPATEN MALANG, JAWA TIMUR Zubaidah, Anis; Hariyadi
Jurnal Abdi Insani Vol 11 No 3 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i3.1834

The development of swift water cultivation systems provides various advantages, including high dissolved oxygen, sufficient natural feed, high stocking density, and lower production costs. This technology can be developed among fish farming groups, especially those who have access to extensive water sources. The implementation of this community service program aims to distribute koi fish cultivation technology using a fast water system to the community, especially in the Boonpring tourist area, Sanankerto Village, Malang Regency. The method used is to provide assistance to partners in intensive koi fish cultivation technology using a fast water pond system. The results obtained are the implementation of community service activities carried out by the activity implementation team at the Boon Pring Tourism Village, as well as increasing public understanding regarding optimizing the management of koi fish cultivation land using a fast water system. The survey results show that there has been a 30% increase in public understanding regarding fast water system koi fish cultivation. The conclusion of this activity is that the technology for cultivating koi fish using a fast water system has been successfully implemented at the Boonpring tourist attraction, Sanankerto Village, Malang Regency.

Effectiveness of kepok banana stem extract (Musa Acuminata) on the immune response of catfish (Clarias gariepinus) infected with Aeromonas hydrophila Hany Handajani; Anis Zubaidah; Dina Izzah Kamila
Depik Vol 13, No 3 (2024): DECEMBER 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.3.34892

Catfish have high economic value, fast growth, and are easy to cultivate. This is the reason why catfish cultivation is increasing. However, there are still obstacles in the cultivation process, namely the threat of disease, one of which is the Motile Aeromonas Septicaemia (MAS) disease caused by Aeromonas hydrophila bacteria. The countermeasure can be done by using active compounds found in plants, including banana stems. This study aimed to determine the effectiveness of giving banana stem extract to carp culture media and treating A. hydrophila disease. This research used an experiment with a completely randomized design. The treatments tested were the addition of banana stem extract at a dose of 5% (treatment A), 10% (treatment B), 15% (treatment C), and antibiotics (treatment K-), and without extract treatment (treatment K+). Each treatment was repeated three times. The results showed that the administration of banana stem extract significantly affected the fish's erythrocytes, leukocytes, and blood glucose. Leukocyte differential observation showed that kepok banana stem extract had a significant effect on eosinophils, lymphocytes, and neutrophils but no significant effect on monocytes. In analyzing fish blood, the optimal values were 7.640.190a cells/mm3 for leukocytes, 3.600.116d cells/mm3 for erythrocytes, 656.028b mg/dl for glucose, 74.670.577d% for lymphocytes, 5.670.577a% for neutrophils, and 00a% for eosinophils. The results indicate that treatment B with 10% kepok banana stem extract was the most effective in administering the extract to catfish infected with A. hydrophila bacteria.Keywords:Dumbo catfishLeukocytesErythrocytesGlucoseMusa AcuminataAeromonas hydrophila

Spatial distribution of Microplastic Abundance in Pateguran Estuary, Pasuruan, East Java, Indonesia Prastyo Hendro Dwi Cahyono; Hariyadi Hariyadi; Anis Zubaidah
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 7 No. 2 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v7i2.12318

Indonesia ranks 2 countries with a high level of plastic pollution. Plastic waste entering the Indonesian sea is estimated to reach 0.48-1.29 million metric tons/year. This microplastic is the result of the degradation of plastic waste and textile waste that decomposes to small size in a very long time . The river is the headwaters of various rivers and streams which have the potential to carry plastic particles . This makes it likely that the waters of the north sea are also polluted by microplastics, one of which is sourced from rivers . This research was conducted to find out how contaminated the northern sea waters by microplastics, especially estuary areas. The area sampled to be studied is one of the river estuaries in Pasuruan, at the Pasuruan river estuary which is then analyzed at the Laboratory of Fisheries, University of Muhammadiyah Malang . The results of the analysis found three types of microplastics, fiber, film, and fragments. Station 2 is the sampling area of the middle estuary area which results in the most abundance of microplastic abundance of 611111.1 particles/m3 . Microplastic types found were dominated by fiber types up to 55%. The most common microplastics found in the estuary waters of the Pateguran river are microplastics with sizes ranging from 1-333.3 µm . Based on FTIR results, it is suspected that there are microplastics derived from nylon or polyamide (PA) and polypropylene (PP) types.

Analysis of water quality and the occurrence of Infectious Myonecrosis Virus (IMNV) in intensive Vannamei Shrimp (Litopenaeus Vannamei) farming Frans Yudistira; Anis Zubaidah; Hariyadi Hariyadi
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 7 No. 2 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v7i2.13279

Water quality plays an important role in maintaining the health and survival of cultured vannamei shrimp (Litopenaeus vannamei). This study aimed to analyze water quality parameters and determine differences in the occurrence of Infectious Myonecrosis Virus (IMNV) between two intensive vannamei shrimp farming companies. The study was conducted in two intensive shrimp farming companies located in North Lombok Regency, West Nusa Tenggara Province. Water quality parameters were observed during the culture period, and the data were analyzed using an independent-samples t-test. The results indicated that water quality conditions were generally suitable for intensive vannamei shrimp culture. However, nitrite concentrations increased when pond siphoning was not performed on time. The independent-samples t-test showed no significant difference in the occurrence of myo virus disease or IMNV associated with water quality between Company A and Company B (0.898 > 0.05). The absence of a significant difference may be related to the geographical proximity of the two companies, which are located within the same regency and obtain seawater from the same coastline. Consequently, both farms may experience similar water-quality conditions and may be exposed to the same disease or virus simultaneously or alternately.

Effect of Binahong Leaf Powder (Anredera Cordifolia) supplementation in feed on the growth performance of Vannamei Shrimp ( Litopenaeus Vannamei ) Anis Zubaidah; Dony Prasetyo; Novia Budii Lestari
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 7 No. 2 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v7i2.45251

Vaname shrimp (Litopenaeus vannamei) is a fishery commodity that has economic value. Vaname shrimp demand from year to year continues to increase. Increasing shrimp demand can be surmounted by accelerating shrimp growth. The purpose of this study was to know the best dose which most effective of binahong leaves powder in feed on the growth performance of vaname shrimp. Shrimp used are sized 5-6 grams. This study uses a Completely Randomized Design (CRD) consisting of 5 treatments and 3 replications to obtain 15 experimental units. In the control treatment without Binahong leaf powder, treatment 1 (10 grams/kg feed), treatment 2 (20 grams/kg feed), treatment 3 (30 grams/kg feed), and treatment 4 (40 grams/kg feed). The parameters measured are ADG / daily growth, absolute length growth, BWG / body weight gain, Mean Body Weight (MBW), survival rate, amount of feed consumption, feed efficiency, Feed Conversion Rate (FCR), and moulting frequency. The results of the study showed that the addition of Binahong leaf powder in feed significant effect (P<0,05) on the ADG / daily growth, absolute length growth, BWG / body weight gain, and feed efficiency, but not significcant effect on the Mean Body Weight (MBW), survival rate, amount of feed consumption, Feed Conversion Rate (FCR), and moulting frequency. The best results are obtained on the treatment 3 (30 grams/kg feed) with the value of ADG / daily growth harian 0,72 grams, absolute length growth 2,75 cm, BWG / body weight gain 92%, Mean Body Weight (MBW) 8,36 grams, survival rate 97,76%, FCR 1,24 grams, amount of feed consumption 113,43 grams, feed efficiency 42,33 grams and moulting frequency 18 shrimps

Effectiveness of red Chili Pepper Juice addition (Capsicum Annuum) on the growth and red color intensity of Comet Goldfish (Carassius Auratus) Anis Zubaidah; Ganjar Adhywirawan Sutarjo; Hikmah Perwita Sari
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 7 No. 1 (2024): February
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v7i1.45255

The quality of ornamental goldfish is highly influenced by body color brightness, which depends on carotenoid content. Since fish cannot synthesize carotenoids naturally, supplementation through feed is required to enhance pigmentation. Cayenne pepper is a natural carotenoid source that has potential to improve fish coloration. This study aimed to evaluate the effect of cayenne pepper extract supplementation on the red color intensity, growth, and survival rate of Comet goldfish. The research employed an experimental method using a Completely Randomized Design (CRD) with four treatments and three replications. The treatments consisted of different cayenne pepper extract concentrations in feed: 0% (control), 1%, 3%, and 5%. Comet goldfish with an average size of ±6 cm were stocked at a density of 10 fish per aquarium and fed three times daily at 5% of biomass. Water quality management, including siphoning and partial water exchange, was conducted every three days. The results showed that cayenne pepper supplementation significantly improved the red color intensity of Comet goldfish. The highest color intensity was obtained in the 5% treatment (22.77 ± 4.96). The highest length growth was also recorded in the 5% treatment (0.50 ± 0.13 cm), while the highest weight gain was observed in the 3% treatment (1.43 ± 0.07 g). The highest survival rate was achieved in the 5% treatment (96.67%). Water quality remained within suitable ranges throughout the study. These findings indicate that 5% cayenne pepper supplementation effectively enhances coloration, growth, and survival of Comet goldfish.

Effectiveness of wet, semi-wet, and dry transportation systems for Koi Fish (Cyprinus Carpio) fingerlings Muhammad Jurais Akasah; Anis Zubaidah; Ganjar Adhywirawan Sutardjo
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 8 No. 1 (2025): February
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v8i1.13173

Koi fish (Cyprinus carpio) are highly popular ornamental fish among hobbyists in both domestic and international markets. However, the concentration of koi farming activities in East Java creates challenges for distributing fish to other regions, both within and outside Java. This study aimed to determine the most efficient transportation system among wet, semi-wet, and dry systems for transporting koi fish fingerlings. The study was conducted over 36 days at a koi fish facility located at Jl. KH. Asyari No. 96, Bondowoso. The equipment used included a digital scale, air stones, aeration tubing, a thermometer, a dissolved oxygen (DO) meter, universal pH strips, a camera, an oxygen cylinder, and a blower. The materials consisted of 60 healthy koi fingerlings measuring 5–7 cm, packaging bags, and labels. An experimental method using a Completely Randomized Design (CRD) was applied, consisting of three transportation treatments with two replications. For 5-cm fish, the wet system used 7–8 L of water, the semi-wet system used 5 L of water, while the dry system used no water and instead employed damp foam placed at the bottom of the bag. Data were analyzed using analysis of variance (ANOVA) at P > 0.05, with no further post hoc test conducted. The results showed that the dry system using damp foam resulted in the highest mortality compared with the wet and semi-wet systems. Water quality was monitored before transportation and during the five-day rearing period. Water quality conditions contributed to mortality in the dry system, particularly in the first replicate on days 3 and 4, with a total of seven mortalities. The study concluded that closed transportation using the wet system was the most effective, with a survival rate of 90%.

Effectiveness of kepok banana stem extract (Musa Acuminata) on the immune response of catfish (Clarias gariepinus) infected with Aeromonas hydrophila Hany Handajani; Anis Zubaidah; Dina Izzah Kamila
Depik Vol 13, No 3 (2024): DECEMBER 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.3.34892

Catfish have high economic value, fast growth, and are easy to cultivate. This is the reason why catfish cultivation is increasing. However, there are still obstacles in the cultivation process, namely the threat of disease, one of which is the Motile Aeromonas Septicaemia (MAS) disease caused by Aeromonas hydrophila bacteria. The countermeasure can be done by using active compounds found in plants, including banana stems. This study aimed to determine the effectiveness of giving banana stem extract to carp culture media and treating A. hydrophila disease. This research used an experiment with a completely randomized design. The treatments tested were the addition of banana stem extract at a dose of 5% (treatment A), 10% (treatment B), 15% (treatment C), and antibiotics (treatment K-), and without extract treatment (treatment K+). Each treatment was repeated three times. The results showed that the administration of banana stem extract significantly affected the fish's erythrocytes, leukocytes, and blood glucose. Leukocyte differential observation showed that kepok banana stem extract had a significant effect on eosinophils, lymphocytes, and neutrophils but no significant effect on monocytes. In analyzing fish blood, the optimal values were 7.640.190a cells/mm3 for leukocytes, 3.600.116d cells/mm3 for erythrocytes, 656.028b mg/dl for glucose, 74.670.577d% for lymphocytes, 5.670.577a% for neutrophils, and 00a% for eosinophils. The results indicate that treatment B with 10% kepok banana stem extract was the most effective in administering the extract to catfish infected with A. hydrophila bacteria.Keywords:Dumbo catfishLeukocytesErythrocytesGlucoseMusa AcuminataAeromonas hydrophila

UTILIZATION OF DRY NOODLE WASTE AS SUBSTITUTION OF WHEAT FLOUR AGAINST GRWOTH AND FEED EFFICIENCY ON TILAPIA (Oreochromis niloticus) Muchamad Nanafi Nurlaksana; Samsundari Sri; Anis Zubaidah
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 6 No. 1 (2023): February
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v6i1.6052

Fish was one of the result waters consumed by many Indonesians. Tilapia (Oreochromis nilaticu) is one of the freshwater fishery products that people in demand, tilapia has a delicious taste, One of the factors that affect the growth of cultivated fish is feed. The cost of feed can reached 60-70% of production cost component. This research was conducted at Fisheries Laboratory of Faculty of Agriculture - Animal Husbandry University of Muhammadiyah Malang. The method used in this research is an experimental method using Completely Randomized Design. In the study there were 5 treatments with the addition of different dry noodle flour that is 0%, 25%, 50%, 75% and 100% and one treatment of noodle control use flour whear, each treatment was repeated four times. The pellet feed is administered adlibitum for 40 days. The main parameters used include the Specific Growth Rate (SGR), Total Feed Consumption (JKP) and Feed Efficiency (EP) were then analyzed using ANAVA and continued with BNT testThe results showed that the effect of Pellet form feeding was very significant (**) on Specific Growth Rate (SGR) Calculate 11.102> from F.Table 5% (3.11) 1% (5,03), and Feed Efficiency EP) F. Calculate 20.752> from F.Table 5% (3.11) 1% (5,03). The quality of tilapia in this study ranged from 25-27 0C, dissolved oxygen ranged from 5.2 to 6.7 and the pH ranges from 6.7 to 8.1.The conclusion of this study P5 treatment of 100% dried noodle flour additions is the best feed with the highest SGR 1.54% and the lowest 0.81.Key Words: Pellet-Formed Feed, Specific Growth Rate (SGR), Total Feed Consumption (JKP,) and Feed Efficiency (EP) Tilapia.

Title

Found 40 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 40 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 40 Documents
Search