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Journal : Jurnal NERS

Sap Extract Papaya for Autolitic Debridement Chronic Wound Agus Yuswanto, Tri Johan; Hariyanto, Tanto
Jurnal NERS Vol 10, No 2 (2015): Vol. 10 Nomor 2 Oktober 2015
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.396 KB) | DOI: 10.20473/%oj.Ners102%y296-300

Abstract

Introduction: The high cost of drugs or specific wound dressings for chronic wounds can be healed at least in two weeks depending on the stage of the wound and blood sugar levels. Hence the need for an innovative processing of natural resources (NR) to produce a chronic wound care products that are accessible to all people.  One of the current trends is to use protease enzymes (sap extract papaya) that serves as autolitic debridement in the treatment of chronic wounds. The aims of this research was to indentify characteristics of protease enzyme present in sap of papaya. Method: This study was a descriptive exploratory that doing pure protease enzyme extract of papaya sap. Results: Molecular weight measurement using a kit Pre-stained Protein Markers (Broad Range) for SDS PAGE, Nacalai tesque No. 02525 showed a homogeneous molecular weight that slightly above 28 kD. Measurements of the enzyme levels using NanoDrop Spectrophotometer ND kit-1000 (with a distilled water marker) showed a relatively homogeneous enzyme level with a range between 43% - 61% and 53, 88% in average. The addition of papaya sap extract into each tube containing 7 ml gelatin obtained results: the control group there were clots perfectly on gelatin; groups with the addition of 2 drops (± 0.5 ml) obtained 50% hydrolyzed gelatin; and groups with the addition of 4 drops (± 0.5 ml) obtained 75% hydrolyzed gelatin. Discussion: The results showed that papaya sap extraction synonymous with protease enzyme. Papaya sap extraction activity (protease enzymes) on hydrolyzing gelatin shows a qualitative picture. Therefore, it can be used as autholitic debridement. Further, the following study is essential to identify structure and activity of the enzyme with a more determinant (temperature, time, and dose).Keywords: sap papaya extraction, protease enzymes
Sap Extract Papaya for Autolitic Debridement Chronic Wound Tri Johan Agus Yuswanto; Tanto Hariyanto
Jurnal Ners Vol. 10 No. 2 (2015): Oktober 2015
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.396 KB) | DOI: 10.20473/jn.v10i2.1376

Abstract

Introduction: The high cost of drugs or specific wound dressings for chronic wounds can be healed at least in two weeks depending on the stage of the wound and blood sugar levels. Hence the need for an innovative processing of natural resources (NR) to produce a chronic wound care products that are accessible to all people.  One of the current trends is to use protease enzymes (sap extract papaya) that serves as autolitic debridement in the treatment of chronic wounds. The aims of this research was to indentify characteristics of protease enzyme present in sap of papaya.Method: This study was a descriptive exploratory that doing pure protease enzyme extract of papaya sap. Results: Molecular weight measurement using a kit Pre-stained Protein Markers (Broad Range) for SDS PAGE, Nacalai tesque No. 02525 showed a homogeneous molecular weight that slightly above 28 kD. Measurements of the enzyme levels using NanoDrop Spectrophotometer ND kit-1000 (with a distilled water marker) showed a relatively homogeneous enzyme level with a range between 43% - 61% and 53, 88% in average. The addition of papaya sap extract into each tube containing 7 ml gelatin obtained.Results: the control group there were clots perfectly on gelatin; groups with the addition of 2 drops (± 0.5 ml) obtained 50% hydrolyzed gelatin; and groups with the addition of 4 drops (± 0.5 ml) obtained 75% hydrolyzed gelatin.Discussion: The results showed that papaya sap extraction synonymous with protease enzyme. Papaya sap extraction activity (protease enzymes) on hydrolyzing gelatin shows a qualitative picture. Therefore, it can be used as autholitic debridement. Further, the following study is essential to identify structure and activity of the enzyme with a more determinant (temperature, time, and dose).
Self Affirmation Reduces the Anxiety, LDH and Troponin I in the Clients with Coronary Heart Disease (CHD) Padoli Padoli; Joko Suwito; Tanto Hariyanto
Jurnal Ners Vol. 14 No. 3 (2019): Special Issue
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.763 KB) | DOI: 10.20473/jn.v14i3.17170

Abstract

Introduction: Positive self affirmation is one of the psychological interventions that can be applied to the treatment of coronary heart disease; its effect is currently unknown. The purpose of this study was to prove the effect of self affirmation on anxiety, troponin I and LDH in coronary heart disease patients.Methods: The type and design of the study was quasi-experimental with a non-randomized post-test control group design. Thirty patients with coronary heart disease who were treated in the Camelia room of Dr Soetomo Hospital who had been selected were divided into 2 groups. The first group was given self affirmation twice / day for 20 minutes and the second group had standard care as the control group. After the intervention, anxiety measurements were taken, in addition to the measurement of troponin I and LDH.Results: The results showed that self affirmation reduced anxiety (ρ = 0.03), decreased troponin I (ρ = 0.003) and decreased the lactate dehydrogenase (LDH) levels (ρ = 0.006).Conclusion: Self-affirmation improves the client’s emotions, preventing damage to the heart muscle cells. This is reflected by a decrease in the troponin I and LDH levels which are indicators of heart muscle damage.
Co-Authors A.A. Ketut Agung Cahyawan W Ade Firman Ahda Suhanda Aini Nurul Amalia, Salma Andi Amrullah Andri Budianto Angelika Christin Role Ani Sutriningsih Anjelina Marta Lupa Arahaf Abdi Ardi Panggayuh Ardiwanto Umbu Tanda Ardiyani, Vita Maryah Ari Suryandi Arizqu Irfan Ardiansah Atik Kurniawati, Atik Aurelia Mau Keo Budi Susatia Damaris Arista Lende Dedi Sumadi Diana Diana Doddy Rakhmadi edy suyanto Ekowati Retnaningtyas Elaspriani Elaspriani Elaspriani Kunto Elisabeth Tea Endang Sri Dewi Hastuti Suryandari Erlisa Candrawati Evi Susanti Fasty Aklima Fauzi Riki Hidayat Fedelia Susanti Rebu Felisiana Felisiana Filsa Destiara Fitria Hayati Glorianita A.R De Araujo Hamka Hamka Hari Sukanto Adi Helmi Herawati, Helmi Hirdayanti Finisia Holyness Nurdin Singadimedja Hupitoyo Hupitoyo Hupitoyo, Hupitoyo I Gusti Agung Komang Diafari Djuni Hartawan Iga Kurnia Rohmah Inra Inra Iwan Muliawan Joko Santoso Joko Suwito Lasri Lasri Lisa Wulandari M. Miftahuz Zubaidi Maemunah, Neni Maria Diah Ciptaning Maria Helmiana Muda Marta Ina Kaka Meity Sarah Melkior Silvanus Yopi Balik Miftahur Rahman Mikhayandi John Lede Mira Pratiwi Misda Misda Moh. Fachrizal Rosyid Mudayatiningsih, Sri Mujito Mujito, Mujito Naomi Sakti Daindo Ni Luh Putu Eka Sudiwati Ni Luh Putu Eka Sudiwati Ni Luh Putu Sudiwati Nia Lukita Ariani Nia Lukita Ariani Novita Dewi Nuraisyah Siregar Padoli Padoli Pendrita Melkianus Kabonju Hita Pery Pery Priyo Sasmito Ragil Catur Adi W. Rinda Rinda Rita Mahareni F. Rizhal Hamdani Rudi Febrianto Rudimin Rudimin Selia Wilhelmina Sendra, Eny Setyarini, Arika Indah Sisilia Ndore Sisilia Niman Siti Zubaidah Sri Rahayu Sujati Sujati Sulasmini Sulasmini Sulasmini Sulasmini Sulasmini Sulasmini Swaidatul Masluhiya AF Tatang Irawan Urli Titin Indah Hartini Tri Johan Agus Yuswanto Wahidyanti Rahayu Hastutiningtyas Wahyu Dini Metrikayanto Wahyuningsri . Wahyuningsri ., Wahyuningsri Whaisna Switaningtyas Widiani, Esti Wisundari Wisundari Yanti Rosdiana Yeremias Utun yohana yohana Yohanes Daud Djawa Yohanes Suni Yosefina Dede Moruk Yovita Lai Hane Yudhawati, Sherlyana Kartika Yuliani Yuliani Zakariya Zakariya Zaky Soewandi Ahmad