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Pengaruh Konsentrasi Yeast Saccharomyces Cerevisiae Dan Waktu Fermentasi Terhadap Pembentukan Bioetanol Dari Mikroalga Chlorella sp. Dewi Kusuma Nurmalasari; Sri Rezeki Muria; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 7 (2020): Edisi 1 Januari s/d Juni 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Bioethanol is an alcohol produced from the fermentation process. Bioethanol is also called ethyl alcohol, which is produced through a microbial process, which is the opposite of ethanol synthesized from petrochemical sources. Microalgae Chlorella sp. can be used as raw material for bioethanol by utilizing microorganisms. Microorganisms used to process microalgae Chlorella sp. to become bioethanol is Saccharomyces cerevisiae. The purpose of this study was to determine the effect of Saccharomyces cerevisiae yeast concentration on bioethanol formation and determine the effect of fermentation time on bioethanol formation. This research phase consisted of hydrolysis, inoculum making, bioethanol fermentation, separation and analysis of bioethanol concentration. The variation of bioethanol fermentation time is 1, 2, 3, 4 and 5 days, while the variation of Saccharomyces cerevisiae concentration is 0.2%; 0.4%; 0.6% and 0.8% (w/v). The optimum fermentation results is bioethanol 2% (v/v) or 15.876 g/L. This optimum bioethanol concentration was obtained on the 5th day of bioethanol fermentation with a yeast Saccharomyces cerevisiae concentration of 0.6% (w/v). Keywords: bioethanol, fermentation, microalgae, yeast saccharomyces cerevisiae
Fermentasi Nira Nipah Skala Pilot Menjadi Bioetanol Menggunakan Saccharomyces Cerevisiae Dengan Penambahan Tween 80 Dan Ergosterol Rafly Rafly; Chairul Chairul; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 3, No 1 (2016): Wisuda Februari Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Ethanol consumption of the world for a variety of uses has increased very significantly in recent years. Therefore it is necessary to alternate sources of raw materials to manufacture bioethanol and bioethanol production can be increased. Nypa sap is one of potential materials to be processed into bioethanol. This research was conducted the pilot scale fermentation of nypa sap, using Tween 80 and ergosterol as suplement to obtain high concentration of bioethanol as well as prevent osmosis in cells. This work is aimed to study of bioethanol pilot scale production from nypa sap, to determine effect of fermentation time to bioethanol and to obtain maximum conditions on the process. And to determine effect of Tween 80 and ergosterol. Fermentation was conducted in 70 L fermentor having variations of fermentation time such as 24; 36; 48; 60; 72; 84 and 96 hours respectively, variations addition of Tween 80 of 250; 375; and 500 ml respectively, and ergosterol of 25; 37,5; and 50 gr. Stirring speed of 200 rpm and temperature of fermentation at room temperature (25 - 300C). Ethanol concentration was analyzed by using Gas Chromatography. Tween 80 and ergosterol effect on the activity of Saccharomyces cerevisiae in converting nypa sap into bioethanol. The process of fermentation maximum conditions indicated in the addition of Tween 80 [375 ml] and ergosterol [37,5 g] at the time of 84 hours having the initial sugar concentration of 258,641 mg/ml. Concentration bioethanol obtained in this condition at 16,602% (v/v) or 131.040 mg/ml.Keywords: Bioethanol, Ergosterol, Nypa sap, saccharomyces cerevisiae, Tween 80
Pengaruh Konsentrasi Ammonium Sulfat Dan Waktu Pada Fermentasi Pulp Kakao Menjadi Bioetanol Menggunakan Saccharomyces Cerevisiae Yuthia Aulia Riani; Chairul Chairul; Wisrayetti Wisrayetti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Bioethanol is one of renewable alternative energy source that can be used as an alternative fuel. Bioethanol can be produced from plant containing starch, sugar, and cellulose through a process of fermentation. One of material can be potential as bioethanol feedstock is cocoa pulp that has glucose content 8-14%. The aim of this research was studying the effect of ammonium sulfate concentration as nitrogen source and fermentation time to process cocoa pulp fermentation to produce bioethanol. The steps of this research comprise preparation of fermentation medium, making inokulum of yeast Saccaromycess cerevisiae, and fermentation step. This study varied of ammonium sulfate concentration 0 g/l, 1 g/l, 2 g/l, and 3 g/l and fermentation time of 24, 36, 48, 60, 72, 84, and 96 hours at pH 5, temperature of 30oC, and inokulum size 10% (v/v). Bioethanol concentration was analyzed by using alcoholmeter and glucose concentration was analyzed by Nelson Somogyi method. The fermentation result of cocoa pulp with glucose content of 124,234 mg/ml was showed that the highest bioethanol concentration was 7% or 55,251 mg/ml for using ammonium sulfate of 2 g/l and fermentation time of 72 hours.Keywords: Ammonium Sulfate, Bioethanol, Cocoa Pulp, Fermentation, Saccaromycess cerevisiae
Pengaruh Waktu Inkubasi Terhadap Biokonversi Reject Nanas Menjadi Bioetanol M Ridwan Afitra; Adrianto Ahmad; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Bioethanol can be produced through a fermentation process materials containing starch, sugar and cellulose fibers by fermentation by microorganisms. Bioethanol can be used as solvents, materials manufacture of perfumes, flavorings, food coloring, and drugs, and even can be used as an alternative fuel. Bioethanol production is done by a process bacth or also called bulk process. The purpose of this study was to determine the effect of incubation time and also get the optimum concentration of ethanol in the manufacture of bioethanol from pineapple reject. Stages of the manufacturing process starts from the substrate pineapple reject juice. Effect of incubation time will be done in this study with variations of 24, 48, 72, 96 and 120 hours. The number of cells during the incubation period will be analyzed by measuring the dry weight of the cell. Fermentation will take place over 4 days (96 hours) with a variation of 12, 24, 36, 48, 60, 72, 84 and 96 hours with the help of Saccharomyces cerevisiae. The results showed that the best time of incubation occurred at 24 and 48 hours with each cell weight of 9.8 g/L and 10.1 g/L at 96 hours of fermentation time and 48 hours which produces bioethanol concentration of 7%.Keywords : bioethanol, fermentation, incubation, pineapple reject, saccharomyces cerevisiae
Pengaruh Variasi pH Dan Waktu Pada Pembuatan Bioetanol Dari Sari Kulit Nanas Dengan Menggunakan Zymomonas Mobilis Cece R; Chairul Chairul; Yelmida Yelmida
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 2 (2014): Wisuda Oktober Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Bioethanol is an alternative energy source that has enough potential to replace fossil fuels. Bioethanol can be produced by fermentation of carbohydrate crops . One of the raw materials that can be used is a pineapple peel. Because today is still a waste of pineapple peel untapped. In pineapple peel contained 13.6 % reducing sugar , so as to have good potential to be processed into bioethanol . One way of making bioethanol is by microbial fermentation using Zymomonas mobilis . The fermentation process is influenced by several factors, including pH and time . This study was conducted to determine the optimum pH and time at fermentation process for bioethanol production from pineapple peel juice. The study was conducted by varying the pH of 4.5, 5.0, 5.5 and 6.0 with fermentation time 14 , 23 , 39 , 47 , 65 , and 71 hours using Zymomonas mobilis microbes . Bioethanol yield analysis using a gas chromatograph (GC). The result is the highest ethanol yield was 3.01% at pH 4.5 and fermentation time of 65 hours. Keyword: Bioethanol, zymomonas mobilis, pineapple peel, yield, GC
Penyerapan Logam Fe Dengan Menggunakan Karbon Aktif Dari AmpasTebu Yang Diaktifasi Dengan KOH Roni Roni; Drastinawati Drastinawati; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Bagasse is one of the agricultural or industrial waste that has potential for an adsorbent. Adsorbents produced from bagasse is processed by the pyrolys process. This study aimed to examine the effect of activated carbon absorption of ferrous metals with concentrations variations of KOH and adsorptiontime, and to study the characteristics of the activated carbon produced, among others, ash content and moisture content. This research was conducted in three phases, namely the preparation of raw materials, the manufacture of activated carbon, and the absorption of Fe in FeSO4 solution. Variations in the concentration of KOH used in this study were 0%, 5%, 10%, and 15%. As for the time variation adsorbs used were 30, 60, and 90 minutes. Percent absorption is the best obtained when the concentration of 15% KOH and adsorbs time of 90 minutes is equal to47%.Keywords: adsorbent, adsorption time, bagasse, KOH concentration.
Pengaruh Konsentrasi Inokulum pada Fermentasi Nira Aren (Arenga Pinnata) Secara Aerob Terhadap Konversi Asam Asetat Karim Abdullah; Chairul Chairul; Syelvia Putri Utami
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Acetic acid is an organic chemical compound that gives a sour taste to vinegar and a sharp odor. Acetic acid is obtained from bioethanol using Arenga plant. Microorganism that used for processing the arenga plant into bioetanol was Saccharomyces cerevisiae and microorganism that used for processing bioethanol into acetic acid was Acetobacter aceti. The purpose of this study is to determine the effect of Acetobacter aceti inoculum concentration and fermentation time on acetic acid conversion rates. The research phase consisted of raw material preparation and making of inoculum, fermentation and analysis. The concentration of inoculum used in this study varied at 10%; 15%; and 20%. The fermentation results are taken after 3, 6, 9 and 12 days. Optimal bioethanol fermentation yield 3% (v / v) or 23.67 g / L, sugar conversion 73.17% at 10% inoculum concentration and produce the highest acetic acid 2.56% or 25,54 g / L at 12 days fermentation at concentration 10% inoculum.Keywords: Acetic acid, bioethanol, arenga plant, fermentation, conversion.
Fermentasi Larutan Glukosa Untuk Produksi Etanol Dengan Teknik Immobilisasi Sel Saccharomyces Cerevisiae Yeni Rizki; Syaiful Bahri; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 3, No 1 (2016): Wisuda Februari Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Ethanol or ethyl alcohol (C2H5OH) is an organic compound that is very important in the chemical industry and has many benefits in human life. One way of making ethanol is fermentation. Arising problem in the fermentation process is the inhibition of the ethanol product that will damage the structur of the plasma membrane and cause protein denaturation will result in inhibited microbial growth and lower productivity. In this research, the fermentation process of glucose solution with cell immobilization technique to obtain ethanol at higher levels. This research is aimed to study the process of making ethanol with cell immobilization technique and determine the effect of the initial sugar concentration and fermentation time of the acquisition of ethanol. Fermentation takes place in batch reactor with a volume of 2 liters of fermentation medium, heavy beads of 20% (w/v), pH 5,0. The fermentation process of glucose solution was done at various variation of fermentation time i.e 24, 36, 48, 60, 72, 84 and 96 hours and also various variations of concentration of glucose as the intial sugar i.e 100 mg/ml, 125 mg/ml and 150 mg/ml. The process was stired at speed of 200 rpm and temperature of fermentation at room temperature (25 – 30oC). Ethanol concentrations were analyzed using Gas Chromatography. From the result was obtained that the maximum fermentation process is show at glucose concentration of 150 mg/ml and fermentation time of 60 hours with ethanol concentration of 5,149% (v/v) or 40,647 mg/ml.Keywords : Ethanol, Fermentation, Immobilized Cells, Saccharomyces cereviceae.
Variasi Ukuran Partikel Sorgum Pada Konversi Pati Sorgum Menjadi Bioetanol Dengan Proses Sakarifikasi Dan Fermentasi Serentak David Hamonangan; Chairul Chairul; Irdoni HS
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 2 (2014): Wisuda Oktober Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Sorghum is a resource of grain yield 55-75% starch  composition similar to corn, which is potentially as raw material for production of bioethanol. Research based sorghum bioethanol manufacture carried out using enzymes stargenTM 002 and starch raw materials. The purpose of this study was to determine the concentration of substrate and the best time of fermentation to produce bioethanol. The research method consists of hydrolysis and saccharification processes are aided by enzymes stargenTM 002 followed by a fermentation process using yeast  Saccharomyces cereviceae, ferment at room temperature, pH 4.5, enzymes stargenTM 002 concentration 2.5% and evaporated at a temperature 1000C. substrate concentration used was 40 g/, variable particle size used was 20-40 mesh, 40-60 mesh and 60-80 mesh and time of fermentation pada 30, 36, 42, 48, 54, 60, 66 and 72  hours. The result indicate that the particle size at 60-80 mesh and the optimum time is 48 hours can produce the highest bioethanol concentration of 1.04% (v/v). Keyword: Bioethanol, Enzymes StargenTM 002, Fermentation, Sorghum, Substract Concentrations.
Pengaruh Konsentrasi Gula Dan Waktu Fermentasi Terhadap Yield Asam Asetat Menggunakan Bahan Baku Nira Nipah Dan Bakteri Acetobacter Aceti Futhanul Wewe; Chairul Chairul; Syelvia Putri Utami
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Nipah plants (Nypa fruticans) grown in province of Riau especially in Lubuk Muda vilage, Bengkalis regency with land area about 50 hectares. Nipah produce sap with high sugar concentration which can be used as an alternative material for acetic acid production by fermentation using microorganisms. The purpose of this study was to determine the effect of the initial sugar concentration of nipah sap and the time of acetic acid fermentation on yield of acetic acid using Saccharomyces cerevicae and Acetobacter aceti microorganisms. Variation of initial sugar concentration was 10%, 20% and 30%, while the fermentation time is 1, 3, 5, 7 and 9 days. Acetic acid fermentation process was carried out at pH 6, stirring speed of 200 rpm and 10% inoculum. The results of analysis using the Nelson-Somogyi method revealed that the highest sugar concentration was evaporation of nipa sap 30% (172.7223 g/L). The highest concentration of acetic acid was obtained 59.399 g/L or 5.94% and yield was 51.599%. The concentration and the highest yield was obtained at 30% evaporation and the 9th day of acetic acid fermentation.Keywords : Acetic acid, Acetobacter aceti, fermentation, inoculum, nipah sap
Co-Authors Achmad Noerkhaerin Putra Ade Ayu Oksari Ade Sri Umaiyah Adriani Lestari Adrianto Ahmad Ahmad Dedi Fadillah Ahmad Mursyid Ajma Nouri Alfarisi, Cory Dian Alya Alissa Amun Amri ANDRIA AGUSTA Annisa Novianti Samin Anthoni Agustien Arfa Dinata Ageng Tirtayasya Cece R David Hamonangan Deasy Rahmayuni Desi Heltina Deswati Deswati Dewi Kusuma Nurmalasari Diah Pramushinta Dike Putra Ganda Dina Citra Naomi Dina Citra Naomi Harianja Dina Remina Drastinawati Drastinawati Efrizal Efrizal Eka Trisnawati Elia Simanjuntak Ella Awaltanova Erizal Mukhtar Evelyn Evelyn Fachry Abda El Rahman Farhiz Lagan Fariez Fariez Ferry Lismanto Syaiful Futhanul Wewe Gilang Fathurrahman Gusna Merina Hafidawati, Hafidawati Hauriah Hauriah I Gusti Bagus Wiksuana Ihsanul Khairi Iislamiyah Iislamiyah Imron Sutiono Indra Junaidi Zakaria Intan Oktaviani Irdoni HS Irdoni Irdoni Irdoni S Irvan Fadli Wanda Karim Abdullah Khaira Khaira M Ridwan Afitra Maria Peratenta Sembiring Maulia Rayana Mery Sukmiwati Mohammad Rezky Muhammad Rivai Multi Handriyani Nadhira Iffah Lisandra Nurainas Nurainas Nurmiati Nurmiati Nuvicha Rizqi Yuniva Zikra Oci Khairani Rafly Rafly Reni Kuswanazia Rian Efrizal Riki Ariyadi Riki Irwandi Rizka Sefmaliza Roni Roni Rusnam Rusnam Said Said Shintia Oktaviani Silvia Reni Yenti Sisi Oktadira Kalpatari Sri Budi Sulianti Sri Meilani Sri Rezeki Muria Sunarno Sunarno Suwirmen, Suwirmen Syafira Junia Ardhani SYAIFUL BAHRI Syamsiar, Syamsiar Syelvia Putri Utami Tafrikhatul Walidah Tantri Wilinda Julia Tiara Tiara Tri Murningsih Wahyu Mey Riswanto Winarto Tarigan Wisrayetti Wisrayetti Yelmida Azis Yeni Rizki Yolanda Amalia YULIASRI JAMAL Yuthia Aulia Riani ZA ZA Zozy Aneloi Noli Zuherti Zainul Zuhri Syam