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Phylogenetic analysis of ornamental Pomacentridae from the southern coast of West Java Munatul Khayah Almusyarofah; Romanus Edy Prabowo; Agus Nuryanto
Depik Vol 9, No 1 (2020): April 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Abstract. Pomacentridae has a wide distribution in Indonesia waters. Research on species richness of Pomacentridae has been carried out in some areas in Indonesia. However, there are no data about the relationships of Pomacentridae on the southern coast of West Java. This research aims to know the evolutionary kinship of Pomacentridae ornamental fish in Ujung Genteng and Taman Manalusu Beach, West Java. This research used a survey method with a judgment sampling technique. The parameters measured were the consistency index (CI) and character evolution of the resulting phylogenetic tree. Cladogram reconstructed based on maximum parsimony algorithm with 1000 bootstrap replicates in PAUP software. The result showed that the tree had a CI value of 0.6000 which indicated low homoplasy. Pomacentridae formed a monophyletic group when they compared with Chaetodon collare as outgroup. The Pomacentridae clade divided into two subclades. The first subclade consisted of Abudefduf indicus as a basal species, then Neoglypydodon bonang and Plectroglyphidodon lacrymatus have a closed kinship and diverged after Dascyllus trimaculatus, and then Abudefduf notatus and Abudefduf sexfaciatus have a close kinship and diverged after Abudefduf vaigiensis. The second subclade referred to as a basal group, and within this subclade, Chrysiptera brownriggii has a closely related to Chrysiptera unimaculata and diverged after Chromis opercularis.Keywords: Cladistics, damselfish, meristic, morphometric, Pomacentridae

Abundance and Characteristics of Microplastics Found in The Gastrointestinal Tract of Commercial Marine Fish from Bitung, North Sulawesi, Indonesia Aunurohim Aunurohim; Eka Krisna Risawati; Eka Nur Rahmawati; Nova Maulidina Ashuri; Fione Yukita Yalindua; Putri Saphira Ibrahim; Nita Citrasari; Miftakhul Sefti Raufanda; Romanus Edy Prabowo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 18, No 1 (2023): May 2023
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.719

Microplastic (MP) pollution is an emerging environmental problem that threatens food security, food safety, and human health since it has been reported to be found in commercial fish consumed by humans. Bitung, North Sulawesi, is one of the biggest contributors to capture fishery production in Indonesia. However, there is no data on microplastic pollution in commercial marine fish from Bitung. Therefore, this research aimed to investigate the presence and identify the visual characteristics (color, shape, size) and the polymer type of microplastics found in the gastrointestinal tract of commercial marine fish from Bitung, North Sulawesi. The gastrointestinal tract was extracted using KOH 10%, and the microplastic was observed under a stereo microscope. A total of 753 microplastic particles were found in the gastrointestinal tract of 74 individuals (prevalence 99%), and there was a statistically significant difference in the abundance of microplastics found in the gastrointestinal tract of pelagic and demersal fish. The average number of microplastic particles found in the gastrointestinal tract of pelagic fish (12,24 ± 2,43) is higher than in demersal fish (7,38 ± 3,48). The dominant color and shape of microplastic found in the gastrointestinal tract of the fish were black and fiber, respectively. At the same time, the dominant microplastic size found in the gastrointestinal tract of demersal fish was bigger (1,001-5,000 µm, 39,4%) compared to pelagic fish (150-500 µm, 47%). The Fourier Transform Spectroscopy (FTIR) analysis result shows that microplastics of the same polymer type can be found in the gastrointestinal tract of both pelagic and demersal fish.

Phylogenetic Relationships among Ornamental Achanturid Fish from Ujunggenteng and Taman Manalusu, West Java Afra Nabila; Agus Nuryanto; Romanus Edy Prabowo; Dian Bhagawati; Kusbiyanto Kusbiyanto
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20671

Acanthuridae is a marine fish family with some members are known as ornamental species. Previous studies has been reported the diversity of Acanthuridae. However, there was no study on the phylogenetic relationship of the Acanthuridae from Ujunggenteng and Taman Manalusu southern coast of West Java. The purpose of this study was to provide information about the phylogenetic relationship among ornamental fishes under the Acanthuridae family of Ujunggenteng Beach Sukabumi and Taman Manalusu Beach Garut, West Java. The phylogenetic tree was reconstructed based on morphological characters using maximum parsimony algorithm in software PAUP with 1000 pseudoreplicates and with Halichoeres marginatus selected as an outgroup species. The phylogenetic tree had a consistency index of 0.6429. The value means that the tree was highly reliable due to its low homoplasy. Acanthuridae formed a monophyletic clade compared to the outgroup species by having seven synapomorphic characters. Acanthurid clade was divided into two subclades at generic levels by nine derived characters; three apomorphic characters in Acanthurus and six apomorphic characters in Naso. The tree indicates that Naso brevirostris and Naso lituratus represents basal group, while all species within genus Acanthurus are the most advanced species.Â This study is the first to provide the data about the evolutionary relationship of ornamental acanthurid fish from the southern coast of West Java. Data on closely related species is one of the essential scientific basis on making policies regarding the sustainable use of those closely related species.

Karakteristik morfologi teritip spons Indonesia Sulistiono .; Mujizat Kawaroe; Hawis Madduppa; Romanus Edy Prabowo
Depik Vol 3, No 2 (2014): August 2014
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.3.2.1553

Abstract. Research on the sponge barnacle of Indonesia is very rare, and this study is aimed to describe the morphological characteristics of sponge barnacles and their specific relationship with their sponge host species. This research was a survey and sponge samples were collected by tearing apart any available sponge found in three sampling locations, typically coral reef areas of Weh Island, Seribu Islands, and Karimunjawa Islands. Sponge barnacles contained in sponge samples were observed using stereo microscope and scanning electron microscope, and species identification was determined based on the morphological description of Darwin (1854), Pilsbry (1916), Martin dan Davis (2001), and Kolbasov (1993). Four species of sponge barnacles were found consisting of Acasta cyathus, Acasta fenestrata, Euacasta dofleini, and Membranobalanus longirostrum. Specific relationships of barnacle and its sponge host were found between Euacasta dofleini and Haliclona sp. and between Membranobalanus longirostrum and Suberites sp. respectively. Keywords : sponge; barnacle; acasta; membranobalanus; biodiversityAbstrak. Penelitian teritip spons Indonesia jarang dilakukan sejak kelompok ini pertama kali dideskripsikan hingga saat ini. Tujuan dari penelitian ini adalah untuk mengkaji karakter morfologi teritip spons Indonesia dan untuk mengkaji hubungan spesies spesifik teritip terhadap spons. Pengambilan sampel pada penelitian ini dilaksanakan sejak Oktober sampai Desember 2012 di tiga lokasi yaitu Pulau Weh, Kepulauan Seribu, dan Kepulauan Karimunjawa. Metode penelitian menggunakan metode survei dan titik pengambilan sampel dipilih berdasarkan keberadaan spons. Sampel diamati menggunakan mikroskop stereo dan mikroskop elektron kemudian diidentifikasi berdasarkan deskripsi Darwin (1854), Pilsbry (1916), Martin dan Davis (2001), dan Kolbasov (1993). Hasil penelitian didapatkan empat spesies teritip spons yaitu Acasta cyathus, A. fenestrata, Euacasta dofleini, dan Membranobalanus longirostrum. Terdapat hubungan spesies spesifik antara teritip dan spons inang yaitu antara teritip E. dofleini dan spons Haliclona sp. dan antara teritip M. longirostrum dan spons Suberites sp. Kata kunci : teritip; spons; acasta; membranobalanus; biodiversitas

Phylogenetic analysis of ornamental Pomacentridae from the southern coast of West Java Munatul Khayah Almusyarofah; Romanus Edy Prabowo; Agus Nuryanto
Depik Vol 9, No 1 (2020): April 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.9.1.14722

Abstract. Pomacentridae has a wide distribution in Indonesia waters. Research on species richness of Pomacentridae has been carried out in some areas in Indonesia. However, there are no data about the relationships of Pomacentridae on the southern coast of West Java. This research aims to know the evolutionary kinship of Pomacentridae ornamental fish in Ujung Genteng and Taman Manalusu Beach, West Java. This research used a survey method with a judgment sampling technique. The parameters measured were the consistency index (CI) and character evolution of the resulting phylogenetic tree. Cladogram reconstructed based on maximum parsimony algorithm with 1000 bootstrap replicates in PAUP software. The result showed that the tree had a CI value of 0.6000 which indicated low homoplasy. Pomacentridae formed a monophyletic group when they compared with Chaetodon collare as outgroup. The Pomacentridae clade divided into two subclades. The first subclade consisted of Abudefduf indicus as a basal species, then Neoglypydodon bonang and Plectroglyphidodon lacrymatus have a closed kinship and diverged after Dascyllus trimaculatus, and then Abudefduf notatus and Abudefduf sexfaciatus have a close kinship and diverged after Abudefduf vaigiensis. The second subclade referred to as a basal group, and within this subclade, Chrysiptera brownriggii has a closely related to Chrysiptera unimaculata and diverged after Chromis opercularis.Keywords: Cladistics, damselfish, meristic, morphometric, Pomacentridae

Karakteristik morfologi teritip spons Indonesia Sulistiono .; Mujizat Kawaroe; Hawis Madduppa; Romanus Edy Prabowo
Depik Vol 3, No 2 (2014): August 2014
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.3.2.1553

Abstract. Research on the sponge barnacle of Indonesia is very rare, and this study is aimed to describe the morphological characteristics of sponge barnacles and their specific relationship with their sponge host species. This research was a survey and sponge samples were collected by tearing apart any available sponge found in three sampling locations, typically coral reef areas of Weh Island, Seribu Islands, and Karimunjawa Islands. Sponge barnacles contained in sponge samples were observed using stereo microscope and scanning electron microscope, and species identification was determined based on the morphological description of Darwin (1854), Pilsbry (1916), Martin dan Davis (2001), and Kolbasov (1993). Four species of sponge barnacles were found consisting of Acasta cyathus, Acasta fenestrata, Euacasta dofleini, and Membranobalanus longirostrum. Specific relationships of barnacle and its sponge host were found between Euacasta dofleini and Haliclona sp. and between Membranobalanus longirostrum and Suberites sp. respectively. Keywords : sponge; barnacle; acasta; membranobalanus; biodiversityAbstrak. Penelitian teritip spons Indonesia jarang dilakukan sejak kelompok ini pertama kali dideskripsikan hingga saat ini. Tujuan dari penelitian ini adalah untuk mengkaji karakter morfologi teritip spons Indonesia dan untuk mengkaji hubungan spesies spesifik teritip terhadap spons. Pengambilan sampel pada penelitian ini dilaksanakan sejak Oktober sampai Desember 2012 di tiga lokasi yaitu Pulau Weh, Kepulauan Seribu, dan Kepulauan Karimunjawa. Metode penelitian menggunakan metode survei dan titik pengambilan sampel dipilih berdasarkan keberadaan spons. Sampel diamati menggunakan mikroskop stereo dan mikroskop elektron kemudian diidentifikasi berdasarkan deskripsi Darwin (1854), Pilsbry (1916), Martin dan Davis (2001), dan Kolbasov (1993). Hasil penelitian didapatkan empat spesies teritip spons yaitu Acasta cyathus, A. fenestrata, Euacasta dofleini, dan Membranobalanus longirostrum. Terdapat hubungan spesies spesifik antara teritip dan spons inang yaitu antara teritip E. dofleini dan spons Haliclona sp. dan antara teritip M. longirostrum dan spons Suberites sp. Kata kunci : teritip; spons; acasta; membranobalanus; biodiversitas

Phylogenetic analysis of ornamental Pomacentridae from the southern coast of West Java Munatul Khayah Almusyarofah; Romanus Edy Prabowo; Agus Nuryanto
Depik Vol 9, No 1 (2020): April 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.9.1.14722

Abstract. Pomacentridae has a wide distribution in Indonesia waters. Research on species richness of Pomacentridae has been carried out in some areas in Indonesia. However, there are no data about the relationships of Pomacentridae on the southern coast of West Java. This research aims to know the evolutionary kinship of Pomacentridae ornamental fish in Ujung Genteng and Taman Manalusu Beach, West Java. This research used a survey method with a judgment sampling technique. The parameters measured were the consistency index (CI) and character evolution of the resulting phylogenetic tree. Cladogram reconstructed based on maximum parsimony algorithm with 1000 bootstrap replicates in PAUP software. The result showed that the tree had a CI value of 0.6000 which indicated low homoplasy. Pomacentridae formed a monophyletic group when they compared with Chaetodon collare as outgroup. The Pomacentridae clade divided into two subclades. The first subclade consisted of Abudefduf indicus as a basal species, then Neoglypydodon bonang and Plectroglyphidodon lacrymatus have a closed kinship and diverged after Dascyllus trimaculatus, and then Abudefduf notatus and Abudefduf sexfaciatus have a close kinship and diverged after Abudefduf vaigiensis. The second subclade referred to as a basal group, and within this subclade, Chrysiptera brownriggii has a closely related to Chrysiptera unimaculata and diverged after Chromis opercularis.Keywords: Cladistics, damselfish, meristic, morphometric, Pomacentridae

Effect of Planting Media Modification on Seed Growth and Development of Three Mangroves Species from Jakarta and Kebumen, Indonesia Ardli, Erwin Riyanto; Hanifah, Wahdini; Prabowo, Romanus Edy; Nafis, Bayu Ashila; Widyartini, Dwi Sunu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 2 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.2.285-292

The rehabilitation of mangroves depends on the availability of high-quality, sufficiently prominent seeds. Currently, a lot of mangrove nursery activities continue to employ traditional techniques, which results in low-quality and slowly growing seedlings. The mangroves of Jakarta were selected because they represent a heavily polluted mangrove ecosystem on Java's northern coast, whereas the mangroves in Kebumen, on Java's southern coast, are in the opposite situation. This study proposed to find an approach to supply Indonesia's mangrove restoration projects with adequate seeds. An experimental approach with a complete randomized design and a factorial pattern was used. The variables tested were planting media and three kinds of mangroves: Rhizophora mucronata, Ceriops tagal, and Bruguiera gymnorrhiza. Mangroves from Jakarta are treated with mud, soil media, or a mix of them. A mixture of mud, husk, and husk charcoal is used for six treatments planting media for mangroves from Kebumen. The mangrove seedlings' growth, biomass, and chlorophyll content are the dependent variables. ANOVA was used to evaluate the data with a 95% confidence level, and Duncan's comparison of means test followed. The growth of mangrove seedlings was discovered to be impacted by media type, while the chlorophyll content, dry biomass of roots, stems, and leaves were not affected by this factor. Three species of mangroves differ regarding growth rate, biomass of roots, stems, and leaves, and chlorophyll content. In order to support Indonesia's efforts to rehabilitate mangroves, this research can offer understanding regarding how to select and employ the best media to effectively produce high-quality seeds.

Gamifikasi dalam Pendidikan STEM: Transformasi Pembelajaran dan Pemberdayaan Siswa menuju Industri 5.0 mahendra, christy; Prabowo, Romanus Edy; Paath, David Kritian; Mili, Wilson Nasumi; Annawati, Bergitta Dwi
IPF: Inovasi Pendidikan Fisika Vol. 12 No. 3 (2023): Volume 12 Nomor 3 Tahun 2023
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/ipf.v12n3.p92-100

Industri 4.0 menuntut siswa untuk berpikir kritis dan inovatif melalui pemanfaatan teknologi. Di lingkup sekolah, penerapan metode gamifikasi ke dalam model pembelajaran pada STEM merupakan pendekatan bermakna dan kontekstual yang diterapkan dalam pembelajaran jarak jauh. Pada penelitian ini metode Gamifikasi diterapkan pada mata pelajaran Fisika berbasis Science, Technology, Engineering, and Mathematics (STEM) yang diujikan kepada 74 siswa dengan menggunakan metode System Usability Scale (SUS). Hasilnya, terbukti bahwa metode gamifikasi memberikan dampak positif dalam mengubah cara belajar siswa sebelum menggunakan sistem gamifikasi dibandingkan setelah menggunakan sistem gamifikasi. Penelitian ini mengungkapkan bahwa penerapan metode gamifikasi dalam pembelajaran berbasis aktivitas STEM secara signifikan meningkatkan hasil belajar siswa. Pada tingkat SMP, nilai pembelajaran meningkat sebesar 64%, dan pada tingkat menengah atas meningkat sebesar 76%. Penilaian metode gamifikasi dengan metode SUS menghasilkan skor positif sebesar 70,3 yang menunjukkan efektivitas yang baik. Penelitian ini menunjukkan bahwa gamifikasi merupakan metode yang efektif dan menarik untuk pembelajaran jarak jauh.

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