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All Journal HAYATI Journal of Biosciences Jurnal Veteriner JURNAL KIMIA SAINS DAN APLIKASI Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Pharmaceutical Science and Technology Jurnal Farmasi Klinik Indonesia Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Universa Medicina Acta Pharmaciae Indonesia : Acta Pharm Indo Indonesian Journal of Chemistry Jurnal Ilmiah Keperawatan (Scientific Journal of Nursing) Molekul: Jurnal Ilmiah Kimia Jurnal Ilmu Kefarmasian Indonesia Jurnal Farmasi Sains dan Praktis
Heny Ekowati
Department Of Pharmacy, Faculty Of Health Sciences, Universitas Jenderal Soedirman. Purwokerto (Indonesia)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Universa Medicina

 1 
Combination of three species of Zingiberaceae prevents doxorubicin-induced hepatotoxicity Heny Ekowati; Sarmoko Sarmoko; Retno Widiastuti
Universa Medicina Vol. 32 No. 1 (2013)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2013.v32.11 - 19

Abstract

BACKGROUND Doxorubicin as an anticancer drug has hepatotoxic side effects. Curcuma xanthorrhiza, Curcuma longa and Zingiber officinale are commonly used as herbals in Indonesia and around the world. Several compounds in these plants have antioxidant activities and are known to exhibit protection against doxorubicin-induced toxicities. This study aimed to observe the hepatoprotective effect of a combination of C. xanthorriza, C. longa, and Z. officinale extract on doxorubicin-induced hepatotoxicity in rats. METHODS A total of 28 Wistar male rats were divided into four groups: 1) control group (0.9% NaCl); 2) doxorubicin 5 mg/kg intraperitoneally (ip) four times in 14 days (days 1, 5, 9, 13); 3) doxorubicin + combination of C. xanthorriza, C. longa, and Z. officinale (temulawak, kunyit, and jahe merah, designated as Tekuja) 250 mg/kg/day orally for 14 days; and 4) doxorubicin + Tekuja extract 500 mg/kg/day orally for 14 days. Measurements of parameters based on liver histopathology and the parameters of serum alanine amino transferase (ALT) and aspartate amino tranferase (AST). RESULTS Doxorubicin caused significant elevation in serum ALT and AST enzymes after 14 days of treatment. Rats treated with doxorubicin + Tekuja extract 250 mg/kg/ day showed no histological changes, but had decreased levels of ALT and AST. CONCLUSION This study indicates that the combination of C. xanthorriza, C. longa, and Z. officinale has a protective effect in rats against liver damage induced by doxorubicin
Protective effects of Nigella sativa against 7,12-dimethylbenz [á] anthracene (DMBA) induced carcinogenesis in rats Nur Fatmi Alisah; Hanif Nasiatul Baroroh; Heny Ekowati
Universa Medicina Vol. 31 No. 2 (2012)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2012.v31.88-95

Abstract

BackgroundLiver cancer is the third most common cause of death from cancer worldwide. Recently, natural products have been widely used as an alternative therapy for liver cancer. Previous studies have reported that Nigella sativa has chemopreventive activity in vitro and in vivo. The objective of this study was to evaluate the effect of a chloroform extract of Nigella sativa seeds (NSS) on female rat hepatocytes after administration of 7,12-dimethylbenz [á] anthracene (DMBA). MethodsThe experimental design comprised five groups of rats. Group I (DBMA control group) received oral DMBA at a dosage of 20 mg/kgBW twice weekly for five weeks, while group V (solvent control group) was given corn oil only. The other three groups received DMBA + NSS at dosages of 250 mg/kgBW, 500 mg/kgBW, and 750 mg/kgBW, respectively. Each group consisted 12 rats. The NSS extract dissolved in corn oil was administered daily by the oral route for 2 weeks before and subsequenyly during DMBA tumor induction. At the end of the study, rat livers were collected and stained with hematoxylin and eosin (H&E) and silver staining by the the AgNOR method. ResultsThere was a difference in liver tissue histopathological profile between the NSS, DMBA control, and the solvent control group. AgNOR counts in the DMBA control group, the DMBA+NSS 250 mg/kgBW group, DMBA+NSS 500 mg/kgBW group, and DMBA+NSS 750 mg/kgBW group were 1.79, 1.51, 1.41, and 1.35, respectively. ConclusionNigella sativa seed extract was able to reduce the liver damage and proliferation in rats induced by DMBA administration.
Hepatoprotective effects of celery in diethylene glycol induced toxicity in rats Ekowati, Heny; Waluyo, Syaikah Syahidah; Endriastuti, Nialiana Endah
Universa Medicina Vol. 44 No. 2 (2025)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.172-180

Abstract

Background Diethylene glycol (DEG) is a contaminant in pharmaceutical solvents and potentially induces hepatotoxicity. Celery is hepatoprotective due to its antioxidant properties. The objective of this study was to evaluate the hepatoprotective effects of celery on DEG-induced liver injury in rats. Methods An experimental laboratory study was conducted involving 25 male Wistar rats weighing 200 grams. They were randomized into five groups (5 rats in each group). Group A served as the control group, while Group B received oral administration of DEG at a dose of 3 g/kg BW twice daily for three days, following six days of food and water administration. The other three groups received DEG at 3 g/kg BW following six days of celery extract once daily administration of 100 mg/kg BW (C), 200 mg/kg BW (D), and 400 mg/kg BW (E). After 14 days, all rats were sacrificed. Observations were conducted macroscopically and microscopically using H&E staining. Liver damage was scored, Kruskal-Wallis followed by the Mann-Whitney was used to analyze the data. Results Macroscopic and microscopic examinations of the liver tissue in the celery groups revealed normal histological architecture with a significant reduction in liver damage. The ethanol extract of celery demonstrated hepatoprotective effects, as evidenced by a statistically significant reduction (p<0.05) in the activation of Kupffer cells, sinusoidal dilation, hepatocyte degeneration, and necrosis. Conclusion Celery has a protective effect against DEG-induced liver damage in rats based on the reduction in Kupffer cell activation, sinusoidal dilation, hepatocyte degeneration, and necrosis.
Nephroprotective effect and untargeted metabolomic analysis of celery extract against diethylene glycol induced nephrotoxicity in rats Wijaya, Triyadi Hendra; Ekowati, Heny; Wasito, Hendri; Maryellen, Geeta; Silitonga, Tasya Melinda; Oktavian, Firdaus
Universa Medicina Vol. 44 No. 2 (2025)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.190-200

Abstract

Background Diethylene glycol (DEG) is known for its nephrotoxic effects as a contaminant. Recent investigations suggest that celery extract potentially protects against DEG-induced renal toxicity, warranting an exploration of the non-targeted metabolomic profile of celery in renal tissue. This study aimed to determine celery nephroprotective effects and identify potential biomarkers using an untargeted metabolomic  celery profile in DEG-induced kidney injury in male rats. Methods An experimental study was conducted involving 24 male Wistar rats, randomized into control and three treatment groups receiving DEG at doses of 1, 3, and 5 g/BW for three days. After 14 days, all rats were sacrificed. For the celery nephroprotective and metabolomic study, 27 male rats were randomized into three groups:  control, DEG group at 3g/BW, and DEG-celery group (receiving celery extract 200mg/kg BW single dose for 6 days). After 14 days, blood was tested for creatinine level and untargeted metabolomic analysis using liquid chromatography-high resolution mass spectrometry. One-way ANOVA was used to analyze creatinine data. Results Diethylene glycol at doses of 1 and 3 g/kg BW caused mild renal damage, while 5 g/kg BW resulted in moderate damage. Creatinine levels in the normal, DEG-induced, and DEG-celery groups were 0.55 ± 0.14, 0.71±0.1, and 0.86± 0.17 mg/dL, respectively, but not statistically significantly different between groups (p>0.05). DEG lead tryptophan and phenylalanine levels increased 45-fold and 123-fold, respectively. Celery reduced levels of these metabolites 21-fold and 65-fold, respectively. Conclusion Diethylene glycol led to mild to moderate renal damage. Celery effectively diminished metabolite levels associated with renal damage, specifically tryptophan and phenylalanine.
Co-Authors . Harwoko . Septiyaningsih Ahmad Fawwaz Dafaulhaq Ahmad Fiki Firdaus Ahmad Fiki Firdaus Ahmad Ghifari Ramadhani ANISYAH ACHMAD Anwar, Amalia Meisya Audina, Fuzilestari Nur Budi Rahardjo Devitasari, Arinda DIAH AYU ANDINI Diah Eko Martini Eka Prasasti Nur Rachmani Endriastuti, Nialiana Endah Esti Dyah Utami Eva Vaulina Yulistia Delsy Faradina Qorina Nadzif Febrian Pradana Febrian Pradana Firster Nugroho Fitri Fauziah hayati Fitri Fauziah Hayati Fitriani, Novita Inawanda Hana, Rifa Khairunnisa Hanif Nasiatul Baroroh Hanif Nasiatul Baroroh Hanif Nasiatul Baroroh Harwoko Harwoko Hasyim, Bellia HENDRI WASITO Hendri Wasito HENDRI WASITO Ika Mustikaningtias Indwiani Astuti Irvan Maulana Firdaus Iskandar Sobri Iskandar Sobri Iskandar Sobri KADEK SRI Laksmi Maharani Maryellen, Geeta Masita Wulandari Suryoputri Masita Wulandari Suryoputri Maylani Permata Saputri Mustofa Mustofa Nadzif, Faradina Qorina Nialiana Endah Nur Fatmi Alisah Oktavian, Firdaus Ponco Iswanto Ponco Iswanto Rahman, Brilian Habib Retno Widiastuti Rizky Farihah Sarmoko Sarmoko Septiyaningsih Septiyaningsih Setiawan, Ely Silitonga, Tasya Melinda Suryoputri, Masita Wulandari Trisnowati . Triyadi Hendra Wijaya Tunggul Adi Purwonugroho TUTI EKASARI Undri Rastuti Waluyo, Syaikah Syahidah Warsinah Warsinah Yudhawan, Indra ZULIA HIDAYATI