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All Journal Jurnal Surya Medika PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JURNAL FARMASIMED (JFM) Health Sciences and Pharmacy Journal Jurnal Farmamedika (Pharmamedica Journal) Lombok Journal of Science Jurnal Farmasetis Indonesian Journal of Pharmacy and Natural Product Prosiding SIMKESNAS Acta Holistica Pharmaciana Borneo Journal of Pharmacy Jurnal Sains dan Kesehatan Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan
Jawa La, Elisabeth Oriana
Sekolah Tinggi Farmasi Mahaganesha
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Other

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PENETAPAN KADAR GLUKOSA UBI JALAR UNGU DENGAN METODE SEMIKUANTITATIF DAN KUANTITATIF Ni Ketut Esati; Elisabeth Oriana Jawa La; Ni Ketut Sinarsih
Jurnal Farmamedika (Pharmamedika Journal) Vol 9 No 1 (2024): Jurnal Farmamedika (Pharmamedica Journal)
Publisher : Sekolah Tinggi Teknologi Industri dan Farmasi Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47219/ath.v9i1.311

Abstract

Ubi jalar ungu adalah makanan yang mengandung sumber karbohidrat kompleks yang tepat digunakan sebagai makanan alternatif penderita Diabetes Mellitus, maka sangat penting untuk mengetahui kadar glukosa yang terkandung di dalamnya. Penentuan kadar glukosa dalam makanan dapat ditentukan dengan beberapa metode analisis yang mempunyai tingkat kesulitan yang berbeda pada proses pengerjaannya. Dalam penetapan kadar glukosa sangat diperlukan metode yang sederhana dan praktis sehingga perlu mengetahui kesesuaian hasil dari beberapa metode penelitian. Penelitian ini bertujuan untuk menentukan kadar glukosa pada uji jalar ungu rebus dengan metode uji semikuantitatif menggunakan perbandingan warna yang dihasilkan dari berbagai konsentrasi glukosa dengan sampel serta dilakukan pengujian kuantitatif secara spektrofotometri UV-Vis. Hasil penelitian pada analisis kuantitatif, kadar glukosa pada ubi jalar ungu yang diolah dengan cara direbus memperoleh rata-rata konsentrasi glukosa yaitu 2.896,6 ppm, dan uji semikuantitatif menggunakan pereaksi warna Benedict memperoleh hasil pada rentang 20.000-30.000 ppm. Berdasarkan hasil yang diperoleh, dapat dinyatakan terdapat perbedaan dari hasil metode uji semikuantitatif dan kuantitatif terhadap kadar glukosa ubi jalar ungu rebus.
Pengaruh Formulasi Terhadap Mutu Fisik Body Butter Ekstrak Etanol Kulit Buah Naga (Hylocereus polyrhizus) Sawiji, Repining Tiyas; Jawa La, Elisabeth Oriana; Yuliawati, Agustina Nila
Indonesian Journal of Pharmacy and Natural Product Vol. 3 No. 1 (2020)
Publisher : Universitas Ngudi Waluyo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.898 KB) | DOI: 10.35473/ijpnp.v3i1.501

Abstract

Kulit buah naga memiliki beragam manfaat walaupun sering dianggap sebagai limbah. Kulit buah naga mengandung banyak pigmen warna, salah satunya antosianin yang berfungsi sebagai antioksidan. Kandungan ini mampu mencegah radikal bebas yang menyebabkan penuaan dini maupun penyakit lainnya. Antioksidan yang terdapat didalam kulit buah naga dapat dimanfaatkan sebagai zat aktif sediaan kosmetika tubuh salah satunya body butter. Salah satu faktor yang mempengaruhi stabilitas sediaan body butter adalah emulgator. Penelitian ini bertujuan untuk mengetahui pengaruh formulasi, yaitu adanya variasi konsentrasi kadar emulgator (tween 60 : span 60) terhadap stabilitas fisik body butter. Sediaan dibuat dengan 3 macam konsentrasi emulgator tween 60 : span 60 yaitu 1%, 2%, dan 3%. Ketiga formula diuji stabilitas fisiknya dengan uji organoleptis, homogenitas, pH, daya lekat, daya sebar, dan daya proteksi. Uji stabilitas fisik dilakukan pada minggu I, II, III, dan IV di suhu ruang. Data pengamatan dianalisis statistik dengan taraf kepercayaan 95%. Hasil evaluasi sediaan body butter menunjukkan bahwa variasi emulgator tidak memberikan pengaruh tehadap sifat fisik body butter meliputi organoleptis, homogenitas, pH dan daya sebar. Namun mempengaruhi uji daya lekat dan daya proteksi (p>0.05). Berdasarkan hasil pengujian, didapatkan hasil yaitu formula III lebih stabil dalam penyimpanan selama 4 minggu daripada formula I dan II.Kata kunci : ekstrak kulit buah naga, emulgator, body butter, stabilitas fisikDragon fruit peels has a variety of benefits although it is often considered a waste. Dragon fruit peels contains many color pigments, one of which is anthocyanin which functions as an antioxidant. This content is able to prevent free radicals that cause premature aging and other diseases. Antioxidants found in dragon fruit peels can be used as active ingredients for cosmetics, one of which is body butter. One of the factors that influence the stability of the body butter preparation is an emulgator. This study aims to determine the effect of the formulation, namely the variation in the concentration of emulgator levels (tween 60: span 60) on the physical stability of body butter. The preparations were made with 3 kinds of 60: span 60 emulsifier concentrations of 1%, 2%, and 3%. The three formulas were tested for physical stability by organoleptic, homogeneity, pH, adhesion, dispersion, and protection. Physical stability tests were carried out at weeks I, II, III, and IV at room temperature. Observation data were analyzed statistically with a confidence level of 95%. The results of the evaluation of body butter preparations showed that variations in the emulgator had no effect on the physical properties of the body butter including organoleptic, homogeneity, pH, and dispersion. But it affects the test of adhesion and protection (p> 0.05). Based on the test results, the results obtained are formula III is more stable in storage for 4 weeks than formulas I and II.Keywords: Hylocereus polyrhizus, emulgators, body butter, physical evaluation
Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus polyrhizus) Jawa La, Elisabeth Oriana; Sawiji, Repining Tiyas; Yuliawati, Agustina Nila
Indonesian Journal of Pharmacy and Natural Product Vol. 3 No. 1 (2020)
Publisher : Universitas Ngudi Waluyo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.572 KB) | DOI: 10.35473/ijpnp.v3i1.503

Abstract

Buah naga merah (Hylocereus polyrhizus) merupakan tumbuhan yang sudah banyak dimanfaatkan oleh masyarakat Indonesia secara alami sebagai tanaman kaya antioksidan, sebaliknya pemanfaatan dari kulit buah naga merah belum sepenuhnya dimaksimalkan. Beberapa penelitian yang telah dilakukan menunjukkan bahwa selain daging buah, kulit buah naga merah dapat dimanfaatkan sebagai sumber untuk pengobatan karena kaya akan antioksidan. Hal tersebut dapat digunakan sebagai dasar dan pilihan untuk menghasilkan produk obat tradisional dari kulit buah naga merah. Penelitian ini bertujuan untuk mengidentifikasi kandungan metabolit sekunder yang terdapat pada kulit buah naga merah berupa skrining fitokimia dan kromatografi lapis tipis (KLT) pada senyawa aktif yang disinyalir terkandung didalam kulit buah naga merah yang dapat dimanfaatkan dalam produk kefarmasian. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 96% diperoleh rendemen ekstrak sebanyak 2,96%. Skrining fitokimia dilakukan sebagai uji pendahuluan untuk mengidentifikasi kandungan metabolit sekunder yang terkandung dalam ekstrak kulit buah naga merah, dilanjutkan dengan KLT untuk mempertegas hasil reaksi positif. Hasil skrining dan KLT diperoleh kulit buah naga merah positif mengandung alkaloid, tanin, flavonoid, steroid dan potensial untuk dijadikan obat tradisional.Kata kunci :kulit buah naga merah, skrining fitokimia, KLT, metabolit sekunder Red dragon fruits (Hylocereus polyrhizus) generally has been widely known Indonesian as a plant that rich in antioxidant  however, the Red dragon fruits peel is not fully utilized. Some of studies have been conducted indicating that as well as its fruit flesh, Red dragon fruits peel can be beneficial as a source for treatment and traditional medicinal products since it is also rich of antioxidants.This research aims to identify the content of secondary metabolites on the Red dragon fruits peel  in the form of phytochemical screening and thin-layer chromatography (TLC) in the active compounds which can be utilized in the product of  Pharmacy. Extraction is conducted by maceration using the 96% ethanol solvent in order to produces extracts as much as 2.96 %.  Phytochemical screening is delivered as a preliminary test to identify the content of secondary metabolites contained in the peel of  Red dragon fruit peel and continued with the TLC to confirmed give positive  results. Results of  the screening and TLC obtained that the peel of  Red dragon fruits positive contains alkaloids, tannins, flavonoids, steroids and potential to be used as traditional medicineKeyword : Red dragon fruit peel, phytochemical screening, TLC, secondary metabolites 
KAJIAN SENYAWA AKTIF DAN KEAMANAN TANAMAN OBAT TRADISIONAL DI INDONESIA SEBAGAI ALTERNATIF PENGOBATAN MALARIA Jawa La, Elisabeth Oriana; Kurnianta, Putu Dian Marani
Acta Holistica Pharmaciana Vol 1 No 1 (2019): Acta Holistica Pharmaciana
Publisher : School of Pharmacy Mahaganesha (Sekolah Tinggi Farmasi Mahaganesha)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62857/ahp.v1i1.8

Abstract

Malaria merupakan salah satu penyakit menular dengan tingkat prevalensi yang cukup tinggi di Indonesia. Penyakit malaria menjadi semakin serius karena meningkatnya jumlah parasit malaria (Plasmodium) yang resisten terhadap obat-obat antimalaria. Banyaknya keanekaragaman hayati yang ada di Indonesia memotivasi banyaknya penelitian dan pencarian bahan obat baru untuk berbagai terapi, terutama untuk penyakit malaria. Pemanfaatan tanaman obat sebagai agent antimalaria sudah banyak dilakukan dan dikembangkan. Berdasarkan hasil penelitian, banyak senyawa aktif terkandung dalam tanaman yang diklaim sebagai tanaman antimalaria, baik berupa tanaman utuh, simplisia, maupun ekstrak, dan senyawa isolasi. Artikel review ini menggunakan metode studi literatur dari berbagai referensi tentang pemanfaatan dan penggunaan obat tradisional. Beberapa tanaman obat telah diteliti memiliki efek farmakologi sebagai obat malaria. Tanaman-tanaman yang memiliki efek antimalaria antara lain, Sambiloto (Andrographis paniculata Nees), Mundu (Garcinia dulcis Kurz), Anting-anting (Acalypha indica L.), Johar (C. siamea Lamk), Pasak bumi (Eurycoma longifolia jack), Ketumpang (Tridax procumbens L), Cocor bebek (Kalanchoe blossfeldiana Poelln), dan Talikuning (Anamirta cocculus). Meskipun efek samping dari obat-obatan tradisional relatif kecil, tetapi keamanan obat-obatan tradisional dalam praktik klinis yang baik harus dipertimbangkan. Kata kunci : Tanaman Obat ,Malaria, Plasmodium, Antimalaria, Keamanan Abstract Malaria is one of the infectious diseases with high prevalence. This disease has become extremely serious because of the resistance of its parasites. The abundance of biodiversity in Indonesia spurs a lot of researches for new medicinal ingredients of various treatments, especially malaria. The use of medicinal plants as antimalaria agents has been widely carried out and developed. Based on the results of research about antimalaria agent in the form of whole plants, simplicia, extract and isolated compounds, many active compounds were found in the plants. This article is a literature study to review traditional medicines from various references. Some medicinal plants have been studied to demonstrate pharmacological effects as antimalaria. These plants are for example; Sambiloto (Andrographidis paniculata Nees), Mundu (Garcinia dulcis Kurz), Anting-anting (Acalypha indica L.), Johar (C. siamea), Pasak bumi (Eurycoma longifolia jack), Ketumpang (Tridax procumbens L), Cocor bebek (Kalanchoe blossfeldiana Poelln), and Talikuning (Anamirta cocculus). Although the side effects of the traditional medicines are relatively small, but safety of traditional medicines for their good clinical practice should be considered. Key words: Medicinal plants, Malaria, Plasmodium, Antimalaria, safety
Uji Aktivitas Antioksidan Ekstrak Etanol Daun Rosemary (Rosemarinus officinalis L.) dengan Metode DPPH dan FRAP serta Pengaplikasiannya sebagai Zat Aktif dalam Losion: Antioxidant Activity Test of Rosemary Leaves (Rosemarinus officinalis L.) Ethanolic Extract with DPPH and FRAP Method and Its Application as an Active Substance in Lotion Ni Ketut Esati; Elisabeth Oriana Jawa La; Gusti Ayu Dewi Lestari
Jurnal Sains dan Kesehatan Vol. 4 No. 4 (2022): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v4i4.1129

Abstract

Salah satu tanaman yang mempunyai nilai ekonomis tinggi yaitu tanaman rosemary (Rosemarinus officinalis L.).  Tanaman ini mempunyai aroma dan rasa yang khas dan banyak mengandung senyawa yang dapat dimanfaatkan sebagai antioksidan, antijamur, antivirus, antibakteri, antitumor, antitrombotik, dan antidepresan. Pemanfaatan tanaman rosemary terutama ekstrak daunnya dapat dilakukan dengan terlebih dahulu melakukan pengujian terhadap ekstrak tersebut, sehingga tujuan dari penelitian ini adalah melakukan pengujian kandungan metabolit sekunder, uji aktivitas antioksidan dengan metode DPPH dan FRAP, serta mengaplikasikannya sebagai zat aktif dalam produk lotion. Dari hasil penelitian ini didapatkan rendemen ekstrak etanol daun rosemary sebanyak 16,87%. Hasil pengujian ekstrak tersebut mengandung senyawa metabolit sekunder diantaranya alkaloid, flavonoid, tannin, dan triterpenoid, namun memberikan hasil yang negatif terhadap saponin. Uji aktivitas antioksidan dengan metode DPPH dan FRAP ekstrak menghasilkan nilai IC50 berturut-turut 10,68 ppm dan 51,84 ppm. Dengan demikian ekstrak etanol daun rosemary memiliki aktivitas antioksidan yang sangat kuat dengan pengujian metode DPPH dan aktivitas yang kuat dengan metode FRAP. Produk antioksidan sediaan topikal berupa lotion dengan zat aktif dari ekstrak etanol daun rosemary menghasilkan uji organoleptis yang baik.
Antibacterial activity extract fraction of black garlic against bacteria propionibacterium acnes with disc diffusion method Pandi, Rensiana Kartini; Jawa La, Elisabeth Oriana; Esati, Ni Ketut
Health Sciences and Pharmacy Journal Vol. 6 No. 3 (2022)
Publisher : STIKes Surya Global Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32504/hspj.v6i3.732

Abstract

Black garlic is the result of processing garlic through a heating process at a temperature of 70oC with a relative humidity of 70-80% for 21 days without additional treatment so that the water content decreases. Black garlic has antibacterial activity due to the presence of bioactive compounds from the phenolic and flavonoid groups. This study aims to determine the secondary metabolite content and antibacterial activity of the black garlic extract fraction against Propionibacterium acnes bacteria. Extraction of Black garlic by maceration method used 96% ethanol as solvent and to obtain extract fraction of Black garlic by multilevel fractionation method using n-hexane, ethyl acetate, and ethanol as solvents. To determine the content of secondary metabolites, phytochemical screening was carried out in each fraction. Antibacterial activity test of Propionibacerium acnes used disc diffusion method with various concentrations of n-hexane, ethyl acetate, ethanol fractions 2%, 4%, 6%, 8%, 10%, positive control (clindamycin 1%), negative control and media control. Data analysis was carried out with qualitative, quantitative, and statistical analysis. The results obtained were the extract fraction of black garlic contains secondary metabolites of flavonoids, alkaloids, saponins, and tannins. The results of the antibacterial activity test showed that the garlic extract fraction had antibacterial activity seen from the presence of an inhibition zone. The largest inhibition zone in the n-hexane fraction of 10% concentration of 8.50 ± 0.10 mm was included in the medium category. The positive control was 12.02 ± 1.41 mm and the negative control gave no inhibition zone.
FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN SEDIAAN BODY BUTTER EKSTRAK ETANOL UMBI BIT (Beta vulgaris L.) DENGAN METODE DPPH Repining Tiyas Sawiji; Elisabeth Oriana Jawa La
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 8 No. 1 (2022): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v8i1.533

Abstract

Beetroot (Beta vulgaris L.) is a plant that contains betasianin (purple pigment) and betasantin (yellow pigment) which makes the color of beets dark red to purplish. The fruit contains a small amount of flavonoid compounds (anthocyanins) which have antioxidant activity. This study aims to develop a body butter formulation of ethanol extract of beetroot (Beta vulgaris L.) as an antioxidant and to see the antioxidant activity with the DPPH immersion method. Extraction of beets used the maceration method using 70% ethanol as a solvent. The antioxidant activity test formula was measured using UV-Vis spectrophotometry with a wavelength of 520 nm. The results showed that formula I obtained an IC50 value of 240 µg/mL (AAI = 0.16), formula II obtained an IC50 value of 139.93 µg/mL (AAI = 0.28), and formula III obtained an IC50 value of 133.34 µg/mL (AAI = 0.29). The higher the antioxidant concentration of the fruit ethanol extract body butter preparation, the smaller the IC50 value, and the greater the AAI value obtained. Based on the results obtained, the antioxidants from the beetroot ethanol extract body butter were still able to fight free radicals at a weak level.
FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN BODY LOTION EKSTRAK KOPI ROBUSTA (Coffea canephora) DENGAN METODE DPPH (2,2-difenil-1-pikrilhidrazil): English; Indonesia Repining Tiyas Sawiji; Elisabeth Oriana Jawa La; I Komang Tri Musthika
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 8 No. 2 (2022): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v8i2.629

Abstract

The skin is the outermost part of the body and is very susceptible to exposure to free radicals. This can be prevented by using cosmetics that contain antioxidants. Coffee beans have the chemical compound chlorogenic acid which belongs to the phenolic group which is useful as an antioxidant. The purpose of this study was to formulate and test the antioxidant activity of Robusta coffee extract body lotion preparations.The method used is pure experiment and control class as a comparison. Analysis of physical quality data was carried out by means of descriptive analysis using tables based on the results of the average calculation of the physical quality test, antioxidant test using DPPH method, and statistical processing of hedonic test data using the One Way Anova test and continued with the Duncan test. The results showed that FII and FIII could be formulated into body lotion preparations and met the physical quality test requirements. The antioxidant test before storage obtained the IC50 value at FI of 47,61 ppm (AAI = 0,84); FII of 43,86 ppm (AAI = 0,91); and FIII of 34,07 ppm (AAI = 1,17) and after FI storage of 49,95 ppm (0,80); FII of 45,47 (AAI = 0,88); FIII of 36,11 ppm (AAI = 1,11). The results of the hedonic test showed that in the parameters of colour, aroma, texture, and comfort of use there were significant fifferences (<0,05), while the parameters of humidity and ease of rinsing alowed insignificant differences (>0,05). Based on the results obtained, Robusta coffee extract body lotion is able to ward off free radicals in the medium – strong category.
OPTIMASI KOMPOSISI SETIL ALKOHOL DAN PROPILENGLIKOL PADA SEDIAAN LOTION TABIR SURYA EKSTRAK ANGGUR HITAM (Vitis vinifera L.) DENGAN METODE SIMPLEX LATTICE DESIGN Repining Tiyas Sawiji; Elisabeth Oriana Jawa La; Fitriyani
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 10 No. 2 (2024): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v10i2.790

Abstract

Black grapes have natural antioxidants which contain flavonoid compounds, namely resveratrol which acts as a UV absorbent so that it can be used as a sunscreen. To facilitate its use, cosmetic preparations are made in the form of lotions. The physical properties and stability of lotion preparations are influenced by emulsifiers and humectants which can maintain the water content when used. The aim of this study was to determine the composition of cetyl alcohol and propylene glycol to produce a formula with optimum physical properties and SPF value. This study used a laboratory experimental research design using the active ingredient of black grape extract (Vitis vinifera L.) at a concentration of 3%. The sunscreen lotion was made in 8 runs with a concentration range of 2-5% cetyl alcohol and 7-10% propylene glycol. The composition of cetyl alcohol and propylene glycol was optimized using Design Expert software version 13 with test parameters of spreadability, adhesion, and viscosity, as well as in-vitro SPF values ​​using a Uv-Vis spectrophotometer. The optimal formula is determined using a numerical method, then intelligence and re-tested its physical properties with predicted values ​​that have been set by the software. The results showed that the optimum formula for black grape extract lotion was 2.1% cetyl alcohol and 9.8% propylene glycol. Black grape extract lotion is in the form of a semi-solid dosage form, with a homogeneous light brown color, and a pH according to the skin. The optimum black grape extract lotion produced a spreading power value of 53.7 g.cm/minute, an adhesion of 1.4 seconds, a viscosity of 142 dPa.S, and an SPF value of 4.6. Based on the research, it can be concluded that the emulsifier and humectant components have an effect on the physical quality of the preparation, including viscosity, adhesion, spreadability.
Total Flavonoid Levels in n-hexane and Ethyl Acetate Fractions of Rosmarinus officinalis L. Leaves and Their Antibacterial and Antioxidant Activities Esati, Ni Ketut; La, Elisabeth Oriana Jawa; Sudiasih, Ni Putu; Saniasih, Ni Nyoman Dina
Borneo Journal of Pharmacy Vol. 7 No. 1 (2024): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v7i1.4034

Abstract

The rosemary (Rosmarinus officinalis L.) is a plant of the Lamiaceae tribe that has not been widely studied regarding its pharmacological activity, known from previous studies to contain secondary metabolites of flavonoids. Flavonoids are phenol compounds with many pharmacological activities, including antibacterials and antioxidants. This study aims to determine the total flavonoid levels in R. officinalis leaves and their effect on antibacterial and antioxidant activities. This research began with the preparation of ethanol extract from R. officinalis leaves, then the fractionation of the extract produced n-hexane and ethyl acetate fractions. Total flavonoid levels were determined against both fractions by UV-Vis spectrophotometry. A test of the fraction’s antibacterial activity against Staphylococcus aureus was performed using the disc diffusion method. The antioxidant test is carried out by the DPPH method. The total flavonoid content of the ethyl acetate fraction is 47.437 ± 1.947%, higher than the n-hexane fraction. Test antibacterial and antioxidant activity showed more significant results in the ethyl acetate fraction than in the n-hexane fraction. In conclusion, the total flavonoid levels of ethyl acetate fraction are directly proportional to the antibacterial and antioxidant activities of R. officinalis leaves.
Co-Authors Agustina Nila Yuliawati Anak Agung Putra Riana Prasetya Arimurni, Dewa Ayu Esati, Ni Ketut Firmansyah, Tiar Fitriyani Gusti Ayu Dewi Lestari I Gede Krisna Yudiarta I Komang Tri Musthika Made Dwi Pradipta Wahyudi S Ni Ketut Sinarsih Ni Ketut Sukarmini Ni Komang Eni Astari Ni komang yoni widyawati Ni Made Rai Yuliani Ni Putu Ratna Mulia Adnyani Ni Putu Sudiasih, Ni Putu Pandi, Rensiana Kartini Prasetya, Anak Agung Ngurah Putra Riana Putu Dian Marani Kurnianta Putu Indra Cyntia Dewi Rensiana Kartini Pandi Salma, Cut Asyifa Baezura Mangkurat Saniasih, Ni Nyoman Dina Sastrawan, I Ketut Putra Sawiji, Repining Tiyas