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All Journal Jurnal Penelitian Pertanian Terapan Jurnal Agrotek Tropika International Journal of Applied Sciences and Smart Technologies Jurnal Pengabdian Nasional Proceeding Applied Business and Engineering Conference Journal of Horticulture Production Technology
Lisa Erfa
Jurusan Budidaya Tanaman Pangan Politeknik Negeri Lampung
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RESPON ASAL BIBIT SETEK DAN JENIS MEDIA TANAM TERHADAP PERTUMBUHAN TANAMAN KRISAN (Chrysanthemum sp.) VAR. ARMITA PADA TAHAP AKLIMATISASI Salsabilla, Nanda Aulia; Tiara, Dede; Maulida, Desi; Erfa, Lisa
Journal of Horticulture Production Technology Vol 2 No 2 (2024)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jhpt.v2i2.3729

Abstract

In vitro culture method can be used to obtain superior chrysanthemum seedlings. The objective of this research is to determine the origin of the cuttings and the best type of planting media for the growth of chrysanthemum seedlings at acclimatization stage. Data collection for this research was carried out in March-May 2024 at Lampung State Polytechnic Ornamental Plant Net House. This research used a Randomized Block Design (RAK) with two factors, namely origin of cuttings seedlings and type of planting medium with 6 treatment combinations which were repeated 4 times. The first factor are origin of the cutting seedlings (P1) top of the plant, and (P2) middle part of the plant. Meanwhile, the second factor are type of planting medium (M1) husk charcoal, (M2) goat pen compost, and (M3) cocopeat. The results of this research show that the use of shoot cuttings with cocopeat and charcoal husk planting media showed better results in all parameters.
Response of Shoots from Porang Leaf Bulbs to Cytokinins and IAA in Shoot Multiplication In Vitro Erfa, Lisa; Maulida, Desi; Hamiranti, Rahmadyah; Ferziana, Ferziana; Yuriansyah, Yuriansyah; Safitri, Betari; Yeni, Yeni
International Journal of Applied Sciences and Smart Technologies Volume 06, Issue 1, June 2024
Publisher : Universitas Sanata Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/ijasst.v6i1.7533

Abstract

The growth regulators in media have the potential to significantly influence the process of in vitro plant regeneration. The response of explants in forming shoots also depends on the type and the combination of growth regulators. This research aimed to identify the most optimal type and concentration of cytokinin for the multiplication of in vitro porang shoots. Additionally, it determined whether the addition of cytokinins should be accompanied by auxin/IAA for porang shoot multiplication. To achieve these objectives, a completely randomized design with six replications was employed, followed by a 5% BNJ. The tested treatments consisted of various combinations of growth regulators. Several observations were recorded, including the speed of explants forming prospective shoots/shoots (days), the age of the explants forming prospective shoots/shoots, the number of prospective shoots and shoots produced per explant, and shoot height (cm). The results showed that 1) Tdz 2 and IAA 0.5 mg.l-1 formed the highest shoots candidates and shoots in porang multiplication in vitro, and 2) the addition of BAP and Tdz 2 mg.l-1 should be combined with IAA 0.5 mg.l-1, while BAP and Tdz 3 mg.l-1 did not require IAA.
In Vitro Regeneration of Dendrobium Through Somatic Embryogenesis from Leaf Explants Hamiranti, Rahmadyah; Prajaka, Nanang Wahyu; Yeni, Yeni; Maulida, Desi; Erfa, Lisa
International Journal of Applied Sciences and Smart Technologies Volume 07, Issue 2, December 2025
Publisher : Universitas Sanata Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/ijasst.v7i2.12705

Abstract

The purpose of this study was to determine the best basic medium type and TDZ concentration for the development of somatic embryos from Dendrobium orchid leaf explants. Three replications were arranged factorial (2x3) in a completely randomized design for this study. First, there were two types of basic media, ½ MS and MS. The second factor was the concentration of cytokinin thidiazuron 1, 2 and 3 mg/L. Each experimental unit consisted of 5 culture bottles, each contained 5 explants. The research results showed that 1) The use of both types of basic media was able to induce callus formation on Dendrobium 'Gradita 31' orchid from leaf explants. 2) The use of 3 mg/L thidiazuron which combined with ½ MS or MS media was able to form primary callus faster than other treatments. 3) The higher percentage for embryo somatic and shoots formation were also found in 3 mg/L thidiazuron.
IN VITRO PLANT REGENERATION FROM CHRYSANTHEMUM CALLUS INDUCED BY GAMMA IRRADIATION AND ISSR GENETIC ANALYSIS Maulida, Dr., Desi; Taisa, Rianida; Erfa, Lisa; Wahyu Prajaka, Nanang; Febria, Dilla; Hamiranti, Rahmadyah; Mubarok, Syariful
Jurnal Agrotek Tropika Vol. 13 No. 4 (2025): JURNAL AGROTEK TROPIKA VOL 13, NOVEMBER 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i4.11828

Abstract

Conventional breeding methods of chrysanthemum plants face several challenges to overcome, and physical mutagens such as gamma rays have proven effective in creating chrysanthemum mutants. As an important tool in flower germplasm innovation and variety improvement, gamma rays have greater penetrating power compared to other breeding methods. This research aims to determine the effect of various doses of gamma irradiation on chrysanthemum callus growth, plant regeneration, and morphology due to induction and to determine the existence of genetic variations using ISSR markers. The experiment consisted of four stages, namely, chrysanthemum callus explants were irradiated at doses of 0, 10, 20, and 30 Gray using gamma rays to induce mutations and observe the effect on callus growth. The irradiation results were planted in BA media 0.5, 1.5, and 3.0 mg L-1 to observe shoot growth. Next, the shoots were planted in rooting media containing NAA with and without activated charcoal (AC) for plant regeneration and amplification of chrysanthemum genomic DNA. The results showed that a lower radiation dose (10 Gray) had a positive influence on all growth parameters, while 30 Gray caused a significant reduction in plant height and number of leaves. NAA 1 mg L-1 AC 2 mg L-1 media gave the best results in supporting the growth of chrysanthemum plants at various levels of radiation exposure. Analysis using ISSR markers confirmed the presence of genetic variation in irradiated plants.
In Vitro Proliferation Of Porang Shoots (Amorphophallus muelleri Blume) In Several Combinations Of Bap And Tdz Hamiranti, Rahmadyah; Erfa, Lisa; Prajaka, Nanang Wahyu; Maulida, Desi; Yuriansyah, Yuriansyah
Jurnal Penelitian Pertanian Terapan Vol 25 No 3 (2025)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v25i3.4633

Abstract

Tissue culture can be used as an alternative technique that can be used to multiply porang seeds to meet the need for seeds in porang development. This study aims to determine the proliferation response of porang in various types and concentrations of BAP or TDZ. The experiment was carried out using a randomized block design (RAK). The treatments tried in the shoot proliferation experiment were P1 (MS 0), P2 (BAP 0.5 mg.l-1), P3 (BAP 1 mg.l-1), P4 (TDZ 0.5 mg.l-1) and P5 (TDZ 1 mg.l-1). Each treatment was tried with 5 replications, and each treatment per replication was planted with 5 bottles. Data were analyzed by Analysis of Variance, and differences between treatments were seen by the LSD test at the 5% level. The use of MS 0 and BAP 1 mg.l-1 gave better results compared to other treatments. The use of MS 0 and BAP 1 mg.l-1 resulted in 100% shoot growth on porang propagules. MS 0 and BAP 1 mg.l-1 produced the highest number of shoots (17.8 and 17.4) and the highest shoot height (3.52 and 2.50) compared to the other three treatments.
PERBANYAKAN TUNAS ADVENTIF PORANG DENGAN MENGGUNAKAN BAP DAN TDZ Erfa, Lisa; Hamiranti, Rahmadyah; Prajaka, Nanang Wahyu; Maulida, Desi
Jurnal Penelitian Pertanian Terapan Vol 25 No 4 (2025)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v25i4.4780

Abstract

The limited availability of conventional porang seedlings through seeds and tubers is a constraint in the development of this plant because it is time-consuming, seasonal-dependent, and produces non-uniform seedlings. Therefore, an alternative method in the form of tissue culture, known as micropropagation, is needed to produce quality porang seedlings. Micropropagation is a more efficient and profitable plant propagation method than conventional methods because it can produce seedlings in a relatively short time, is not seasonal-dependent, uniform, and free from pathogens. This study aims to determine the response of porang shoot multiplication to various types and concentrations of cytokinin growth regulators. The experiment was conducted using randomized block design. The treatments tested were P1 : BAP 0 mg.l-1 + TDZ 1 mg.l-1 ; P2 : BAP 0 mg.l-1 + TDZ 2 mg.l-1 ; P3 : BAP 0 mg.l-1 + TDZ 3 mg.l-1 ; P4 : BAP 1 mg.l-1 + TDZ 0 mg.l-1 ; P5 : BAP 1 mg.l-1 + TDZ 0,5 mg.l-1 ; P6 : BAP 1 mg.l-1 + TDZ 1 mg.l-1 ; P7 : BAP 2 mg.l-1 + TDZ 0 mg.l-1 ; P8 : BAP 2 mg.l-1 + TDZ 0,5 mg.l-1 ; P9 : BAP 2 mg.l-1 + TDZ 1 mg.l-1 ; P10 : BAP 3 mg.l-1 + TDZ 0 mg.l-1 ; P11 : BAP 3 mg.l-1 + TDZ 0,5 mg.l-1 ; dan P12 : BAP 3 mg.l-1 + TDZ 1 mg.l-1. Each treatment was replicated five times, with five bottles planted in each replication. Data were analyzed using Analysis of Variance, and differences between treatments were assessed using the LSD test at the 5% level. The observed variables were the rate at which explants formed buds, the number of buds, and the multiplication rate. Addition of cytokinin affected the multiplication response of porang buds in vitro. The P6 treatment produced the fastest bud formation rate, the highest number of buds and the highest multiplication rate.
Co-Authors Ali, Fahri Desi Maulida dilla febria, dilla Ferziana Ferziana, Ferziana Hamiranti, Rahmadyah Mustika Adzania Lestari Prajaka, Nanang Wahyu Prayuda, Galih Dwi Purnama Sari, Hevia Raida Kartina Rizka Novi Sesanti Safitri, Betari Salsabilla, Nanda Aulia Syariful Mubarok Taisa, Rianida Tiara, Dede Vallepy, Andini Wahyu Prajaka, Nanang Yeni Yeni Yuriansyah Yuriansyah Yuriansyah Yuriansyah, Yuriansyah