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Multiplikasi Mata Tunas Pisang ‘Cavendish’ In Vitro Pada Berbagai Konsentrasi Benziladenin Maulida, Desi; Erfa, Lisa; Sesanti, Rizka Novi
Jurnal Penelitian Pertanian Terapan Vol 18, No 1 (2018)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.958 KB) | DOI: 10.25181/jppt.v18i1.748

Abstract

Banana is one of the most important global agriculture commodities. Asia accounts for the production of bananas amounted to 56.4% of total banana in the world and Indonesia is one of the banana-producing countries in Asia. The demand for bananas increased need is anticipated with good cultivation techniques to meet domestic and international market demand. Plant regeneration in vitro tissue culture techniques or can be used to produce propagules true-to-type in large quantities in a relatively short period and free from disease. This research aims to study the influence of various concentrations of BA (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mg/l) against the growth and reproduction of Cavendish banana propagules in vitro. Time appears buds, propagules length and the number of propagules per explant, and the number of primary roots of plants per explant observed after eight weeks of culture. The experiment results show that after explant grown for eight weeks on the media's treatment shows the different response. The increased concentration of BA can increase the number of shoots. The increased concentration of BA from 0.5 mg/l be 1-3 mg/l increased the number of propagules, but the number of propagules will decrease in the concentration of BA more 3.5 or 4 mg/l. In addition to BA concentration 0.5, 1 and 1.5 mg/l MS media into producing shoot buds appear faster time high, highest propagules and root amount more on banana propagules Cavendish, but the number of shoots is fewer. The best of BA concentrations to induce propagules plant bananas Cavendish is 3.0 mg/l.
Improvement of Soil Chemical Properties and Growth of Maize due to Biochar Application on Ultisol Rianida Taisa; Desi Maulida; Abdul Kadir Salam; Muhammad Kamal; Ainin Niswati
JOURNAL OF TROPICAL SOILS Vol 24, No 3: September 2019
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2019.v24i3.101-107

Abstract

Ultisols have poor soil characteristics, yet biochar is one of the technologies that can be applied as a soil enhancer to improve the soil quality. Biochar has succeeded in improving soil quality, through improving soil physical, chemical, and biological properties. This research aimed to improve the quality of soil chemical properties and growth of maize plant, as well as to find a combination between biochar type and dosage of biochar that is able to improve the chemical properties of Ultisols and/or the growth of maize plant. This research was conducted at the Greenhouse and Soil Science Laboratory, Faculty of Agriculture, University of Lampung. This study used a randomized block design (RBD) arranged in factorial with 3 factors and 3 replications. The first factor was the soil layer (topsoil and subsoil), the second factor was the type of biochar (biochar of cocoa shell and biochar of oil palm shell), and the third factor was the biochar dosage (0, 10%, 20%, and 30% of 10 kg of oven dry weight soil). The results showed that (1) the application of biochar from the  cocoa shell and oil palm shell on Ultisols improved some parts of soil chemical properties, namely CEC, organic C, and Kexc, (2) application of biochar from the cocoa shell and oil palm shell on Ultisols increased the plant height, the number of leaves, and the dry weight of maize, (3) application of biochar from the cocoa shell at a dosage of 20% was significantly improved CEC of Ultisols (4) application of biochar from the cocoa shell in top soil at a dosage of 30% significantly improved the soil organic C of Ultisol, (5) application of  biochar from cacao shell at a dosage of  30%  was significantly improved Kexc, (6) application of biochar from oil palm shell at a dosage of 10% significantly increased maize plant growth.
Pengaruh Pemberian Iba (Indole Butyric Acid) dan Konsentrasi Naa (Naphthalene Acetic Acid) terhadap Keberhasilan Penyetekan Sirih Merah (Piper Crocatum Ruiz and Pav.) Desi Maulida; Rugayah Rugayah; Dewi Andalasari
Jurnal Penelitian Pertanian Terapan Vol 13 No 3 (2013)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.444 KB) | DOI: 10.25181/jppt.v13i3.179

Abstract

The research was conducted at the cutting red betel (Piper crocatum Ruiz and Pav.) to determined the effect of (1) the difference growth of cuttings of red betel which are given IBA and without IBA, (2) the concentration of NAA on growth of cutting red betel, (3) the concentration of NAA on growth of cutting red betel on each given IBA. The treatment was arranged in factorial (2 x 4) in randomized block design with three replication. The first factor were without IBA (a0) and the given of 1.000 ppm IBA (a1). The second factor were the concentration of NAA consists of: 0 ppm (b0), 1.000 ppm (b1), 2.000 ppm (b2), and 4.000 ppm (b3). The results showed that the NAA concentration of 4000 ppm produced the most number of roots on either at the node or at the base of cutting. Planting cutting red betel which given IBA 1.000 ppm was able to accelerated the time leaves open and increased the number of cutting which germinate. The mixtured of giving IBA 1.000 ppm and the concentration of NAA 4.000 ppm, produced the most number of cutting that germinated. Keywords : red betel, given IBA, dan concentration NAA
Keberhasilan Aklimatisasi dan Pembesaran Bibit Kompot Anggrek Bulan (Phalaenopsis) Pada Beberapa Kombinasi Media Tanam Lisa Erfa; Desi Maulida; Rizka Novi Sesanti; Yuriansyah Yuriansyah
Jurnal Penelitian Pertanian Terapan Vol 19 No 2 (2019)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v19i2.1420

Abstract

Aklimatisasi merupakan tahap yang kritis bagi plantlet. Pada lingkungan yang baru plantlet dapat mengalami cekaman lingkungan. Kondisi lingkungan seperti media yang tidak mendukung pada tahap aklimatisasi dapat menyebabkan kematian plantlet.  Penelitian ini bertujuan:  1)  Mendapatkan kombinasi  media yang paling baik bagi keberhasilan aklimatisasi plantlet anggrek Phalaenopsis; 2)Mengetahui apakah terdapat interaksi antara media bagian bawah dengan atas pot; 3) Mendapatkan kombinasi media yang paling baik bagi pertumbuhan bibit anggrek anggrek Phalaenopsis pada tahap pembesaran, dan 4) Melihat apakah media sabut kelapa dapat digunakan untuk aklimatisasi dan pembesaran anggrek Phalaenopsis.  Penelitian dilaksanakan di rumah jaring Politeknik Negeri Lampung dari bulan Maret hingga Agustus 2018.  Plantlet diaklimatisasikan 4 minggu.  Percobaan faktorial dilaksanakan dalam rancangan acak kelompok. Faktor A :  macam media bagian bawah (bata/arang).  Faktor B :  macam media bagian atas (moss sphagnum/pakis/sabut kelapa).  Enam kombinasi perlakuan dicobakan (masing-masing dengan 10 ulangan), yaitu:   P1 (bata+moss); P2 (bata+pakis); P3 (bata+sabut kelapa); P4 (arang+moss); P5 (arang+pakis); dan P6 (arang+sabut kelapa).  Peubah yang diamati: 1)  persen keberhasilan plantlet menjadi bibit, 2) tinggi bibit, 3) panjang daun, 4) lebar daun, dan 5) jumlah daun.   Data yang diperoleh dilakukan analisis ragam dan dilanjutkan dengan uji BNJ 5%.  Hasil penelitian menunjukkan: 1) Media moss atau pakis dikombinasikan dengan bata maupun arang memberikan persen keberhasilan aklimatisasi plantlet anggrek Phalaenopsis yang paling baik (100%); 2) Tidak terdapat interaksi antara media bagian atas dengan bagian bawah; 3) Kombinasi media yang paling baik bagi pertumbuhan bibit anggrek Phalaenopsis pada tahap pembesaran adalah moss baik dikombinasikan dengan bata ataupun arang, kemudian diikuti oleh pakis (dikombinasikan dengan bata ataupun arang); dan 4) Media sabut kelapa dapat digunakan untuk aklimatisasi dan pembesaran bibit anggrek Phalaenopsis bila  dilakukan penyiraman yang lebih intensif.
Kultur Embrio Kelapa Kopyor Menggunakan Beberapa Konsentrasi BA Dan Air Kelapa Desi Maulida; Lisa Erfa; Marveldani
Jurnal Penelitian Pertanian Terapan Vol 20 No 3 (2020)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v20i3.1929

Abstract

Coconut Kopyor is an abnormal fruit, the ordinary coconut flesh is attached to the shell and separated from coconut water while Coconut Kopyor, fruit flesh is not attached to the shell but is mixed with coconut water. Kopyor coconut cannot be used as a seed (seed). The growth of kopyor coconut embryos can only be done in a laboratory with embryo culture technology. Embryo culture is the only way to produce true to type coconut kopyor seeds that can produce 80% kopyor coconut in one kopyor coconut plant, kopyor coconut seedlings produced can support government programs in increasing the productivity of kopyor coconut through the provision of quality seeds. Research on kopyor coconut embryo culture was carried out at the Laboratory of Tissue Culture at Lampung State Polytechnic. The experiment was carried out using a RAL, with 15 treatment combinations of BA media formulations (0, 2, and 4) with coconut water (0 ml / l, 100, 150, 200, 250 ml / l). The observed variables were the percentage of embryos sprouting, when the shoot appeared, shoot height, number of roots, and number of leaves. The results showed that the use of BA 4 mg / l without the addition of coconut water increased the percentage of sprouts, faster time for shoots, and highest shoot height.    
Perkecambahan dan Pertumbuhan Biji Anggrek Phalaenopsis pada beberapa Kombinasi Komposisi Media dan Air Kelapa lisa erfa; Yuriansyah Yuriansyah; Rizka Novi Sesanti; Desi Maulida
Jurnal Ilmiah Inovasi Vol 19 No 1 (2019): April
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jii.v19i1.1148

Abstract

Komposisi media buatan yang digunakan merupakan salah satu faktor yang menentukan kecepatan perkecambahan dan pertumbuhan bibit anggrek dalam botol. Penelitian ini bertujuan untuk: 1) mendapatkan kombinasi komposisi media dan air kelapa yang baik untuk perkecambahan dan pertumbuhan protocorm anggrek Phalaenopsis amabilis; dan 2) untuk mengetahui apakah pupuk daun dapat menggantikan penggunaan media dasar MS dalam perkecambahan biji dan membantu pertumbuhan protocorm anggrek Phalaenopsis. Penelitian dilaksanakan pada bulan September sampai November 2017 di Laboratorium Kultur Jaringan Politeknik Negeri Lampung. Ada 9 kombinasi perlakuan yang diujikan: P1 = MS tanpa penambahan air kelapa, P2 = MS ditambah air kelapa 75 ml. l-1, P3 = MS ditambah air kelapa 150 ml. l-1, P4 = Kristalon Hijau tanpa air kelapa, P5 = Kristalon Hijau ditambah 75 ml air kelapa. l-1, P6 = Kristalon Hijau ditambah 150 ml air kelapa. l-1, P7 = Tanam tanpa air kelapa, P8 = Tanam ditambah 75 ml air kelapa. l-1, dan P9 = Growmore ditambah 150 ml air kelapa. l-1. Pengamatan dilakukan terhadap waktu yang dibutuhkan benih untuk berkecambah dan kecepatan pertumbuhan protokorm hingga siap disubkultur. Hasil penelitian menunjukkan: 1) Komposisi media terbaik untuk perkecambahan dan pertumbuhan protokorm anggrek Phalaenopsis adalah media dasar MS dengan penambahan air kelapa 75 ml. l-1 dilanjutkan dengan media MS dengan penambahan 150 ml air kelapa. l-1; 2) Pupuk Kristalon Hijau dengan penambahan air kelapa 75 ml. l-1 juga 150 ml. l-1 dapat digunakan untuk perkecambahan dan pertumbuhan protocorm anggrek Phalaenopsis menjadi semai, namun dengan waktu yang lebih lama dibandingkan media MS.
Respon Pertumbuhan dan Hasil Pakcoy (Brassica campestris var. chinensis) Hidroponik pada Pemberian Konsentrasi Pupuk NPK dan Pupuk Daun Maudi Sugesti Junior; Rizka Novi Sesanti; Desi Maulida; Sismanto Sismanto; Fahri Ali; Yeni Yeni
Journal of Horticulture Production Technology Vol 1 No 1 (2023)
Publisher : Politeknik Negeri Lampung

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Abstract

Hydroponic crop cultivation generally uses AB mix nutrient. However, the expensive price of AB Mix nutrient caused increasing production costs. In addition to the expensive price, the availability of AB Mix is also limited. As an alternative, there is a need for more affordable fertilizer such as compound fertilizer and foliar fertilizer. The purpose of this study was to (1) determine the best compound fertilizer concentration to growth and yield of Pakcoy; (2) determine the best foliar fertilizer concentration to growth and yield of Pakcoy; (3) determine the interaction between compound and foliar fertilizer to growth and yield of Pakcoy. This study was used Split Plot Randomized Block Design consist 2 replicated. Main plot was compound fertilizer concentration and sub plot was foliar fertilizer concentration.The resulted of this study was (1) 2.5 g/L compound fertilizer concentration provide the best growth and yield of Pakcoy; (2) there was no significant effect of foliar fertilizer concentration; (3) there was no interaction between tho treatnments.
Evaluasi Pertumbuhan dan Hasil Beberapa Kultivar Kubis Bunga (Brassica oleracea var. botrytis L) Dataran Rendah pada Sistem Hidroponik Nutrient Film Technique Umi Listiana; Rizka Novi Sesanti; Desi Maulida; Raida Kartina; Sekar Utami Putri; Betari Safitri
Journal of Horticulture Production Technology Vol 1 No 1 (2023)
Publisher : Politeknik Negeri Lampung

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Abstract

The hydroponic system in a greenhouse for cauliflower cultivation can be a solution to the problem of conventional cultivation, but its needed for the cultivars that are most suitable for hydroponic systems, especially the Nutrient Film Technique (NFT) system. This study aims to (1) evaluate the growth and production yields of several cultivars of cauliflower in the Nutrient Film Technique (NFT) hydroponic system, (2) the best cultivar for cultivation of NFT hydroponic method. This study used a lowland cauliflower cultivars, namely PM 126, Mona, Diamond 40, Orient, and Pertiwi with 5 times. The data obtained from each observation variable were analyzed by means of analysis of variance which was carried out with a follow-up test with Honest Significant Differences (BNJ) at the 5% level. The results showed that cauliflower cultivars had different growth responses to the NFT system. Pertiwi cultivar had the best plant height compared to other cultivars, while the variables of leaf width, leaf length, time of flower emergence, flower weight, flower diameter and root length between cultivars showed no significant difference. The cultivar that shows good production results and is suitable for NFT hydroponic systems is PM126.
Pengaruh Jenis Media Tanam dan Interval Pemberian Pupuk Daun Terhadap Pertumbuhan Plantlet Anggrek Dendrobium Blue Twist x Dendrobium Biggibum pada Tahap Aklimatisasi dan Pembesaran Pretty Aulia Dewi Fortuna; Sismanto Sismanto; Desi Maulida; Lisa Erfa; Ratih Rahhutami; Rahmadyah Hamiranti
Journal of Horticulture Production Technology Vol 1 No 2 (2023)
Publisher : Politeknik Negeri Lampung

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Abstract

Dendrobium orchids have the potential to be developed because they have a variety of types, shapes and sizes. The choice of planting media and fertilization is an important factor in the success of orchid propagation, especially at the acclimatization stage. The aim of this research were determine the type of planting media and the best interval for giving foliar fertilizer and determine the best interactions and combinations of treatment of the type of planting media and the interval for giving foliar fertilizer on the growth of dendrobium orchid plantlets in the acclimatization and enlargement stages. This research used a factorial Randomized Block Design (RAK) with the first factor being the type of planting media and the second factor being the interval of foliar fertilizer application. The first factor includes M1: wood charcoal, M2: cocopeat, M3: wood charcoal + cocopeat 1:1, M4: wood charcoal + cocopeat 1:2, and M5: wood charcoal + cocopeat 1:3. The second factor includes P1: once every 4 days, P2: once every 8 days, P3: once every 12 days. The results of the research showed that the cocopeat planting media type provided the best percentage of acclimatization success for the Dendrobium Blue twist x Dendrobium Biggibum orchids. Providing foliar fertilizer at 8-day intervals provides the best increase in plantlet height and leaf width at the acclimatization and plantlet enlargement stages of Dendrobium Blue twist x Dendrobium bigibbum orchids. There was no interaction between the use of planting media type and the interval of foliar fertilizer application.
Induksi Akar pada Setek Batang Aglaonema sp. Var Siam Aurora dengan Pemberian IBA dan NAA Amalia Hartias Anggraini; Desi Maulida; Lisa Erfa; Rizka Novi Sesanti; Yeni Yeni; Sekar Utami Putri
Journal of Horticulture Production Technology Vol 1 No 2 (2023)
Publisher : Politeknik Negeri Lampung

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Abstract

Stem cutting propagation is commonly used to vegetative propagated of Aglaonema sp, however the growing roots has not been much growth. Nevertheless, the shoots that grow only one to three buds with the length of time shoots and roots appear is about 50-75 days after cuttings. It is all depends on the genotype of the Aglaonema sp. The aims of this study was to (i) determine the effect of IBA and NAA concentration in inducing roots of Aglaonema sp. Var Siam Aurora stem cuttings; (ii) to determine the interaction of IBA and NAA treatments to inducing roots of Aglaonema sp. Var Siam Aurora; (iii) determine the best combination of IBA and NAA to inducing roots of Aglaonema sp. Var Siam Aurora. The experimental design was Randomized Block Design (RBD) Factorial with the first factor is the IBA concentration; 0 mg.l-1(I0) and 1000 mg.l-1(I1). The second factor is NAA concentration; 0 mg.l-1(N0), 500 mg.l-1 (N1), 1000 mg.l-1 (N2), 1.500 mg.l-1 (N3)and 2000 mg.l-1 (N4) and resulting 10 combination treatments of IBA and NAA. The data was conducted with analysis of variance and followed by BNT test at 5% and the variable observed were root length, number of roots, first time of roots growth, first time of buds growth, the percentage of cutting stem. The result showed that the combination of IBA and NAA 1000 mg.l-1 give significant effect to inducing cutting stem of Aglaonema sp. Var Siam Aurora.